Scientists sequence a genome seven times bigger than yours

Using haploid DNA and advanced computer technology, researchers have finally managed to sequence the genome of the loblolly pine tree.

After fitting 16 billion separate fragments together, scientists have finally managed to sequence the genome of the loblolly pine tree, the largest ever genome sequenced so far.

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The scientists, who published their papers in GENETICS and the journal Genome Biology, used DNA extracted from a single haploid seed of a Loblolly pine tree.

To obtain the DNA, the scientists first had to remove the embryo from the seed, says Indiana University's KeithanneMockaitis, an author on the paper. What remains is then a haploid, whose cells have just one set of chromosomes.

Using next-generation sequencing technology, researchers obtained billions of shorter sequence of bases. The challenge now was to sift through the data, identify the overlapping sequences, and assemble them together a computational puzzle called "genome assembly."

In the case of loblolly pine, the huge size of the genome made this process difficult.

The "challenge isn't just collecting all the sequence data. The problem is assembling that sequence into order," said David Neale, a professor of plant sciences at the University of California, Davis, who led the loblolly pine genome project.

"You have this big pile of tiny pieces and now you have to reassemble the book," said Steven Salzberg, professor of medicine and biostatistics at Johns Hopkins University, one of the directors of the loblolly genome assembly team, who was also an author on the papers.

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Scientists sequence a genome seven times bigger than yours