DNA Sequencing – Video

Video Link: https://www.youtube.com/watch?v=Ua5x_aHt94E

DNA Sequencing
Rashmi Okay so we are teaching DNA sequencing (DS) , so obviously the first question that comes into mind is what is DS? Does any one know? **Wait for audience to reply** Simran (Yes/No) DS is any process used to map out the sequence of Nucleotides that make up the DNA Strand, and we use it to completely analyze a particular gene #39;s structure and how it is related to its expression and specific polypeptide production Enghuot The Sanger dideoxy method was developed in 1977 by Frederick Sanger and his colleagues using the principles of DNA replication -- a process that requires a single stranded DNA template, a primer, DNA polymerase, and free nucleoside triphosphates. Rashmi Sanger and his colleagues managed to determined the sequence of the an entire genome of a bacteriophage containing 5386 base pairs. Simran Okay, now we are going to explain the Sanger Dideoxy Method. If you turn to page 301 you #39;ll see what we are talking about. Enghuot The first thing that happens is that the DNA template is heated until the two strands are separated. Rashmi We take one of the strands and add a short, single-stranded radioactively-labeled primer to the end of it. In Sanger #39;s method, 4 reaction mixtures are set up. Each one including a primed single stranded DNA to be sequenced, DNA Polymerase, a supply of nucleotides ACGT, and a small amount of labelled chain terminating variant of one of the nucleotides. Simran The last is known as a dideoxy analogue, this is a dideoxynucleotide ...From:Rashmi PrakashViews:0 0ratingsTime:06:41More inEducation

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DNA Sequencing - Video