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Category Archives: Transhuman News

2 former cyclists deny Puerto court DNA access

Posted: February 13, 2013 at 11:45 am

MADRID (AP) -- Two former cyclists on Tuesday turned down requests to have their DNA analyzed to see if any of the blood bags seized by Spanish police as evidence in the Operation Puerto doping trial could be linked to them.

Isidro Nozal, a 35-year-old former rider with the ONCE, Astana and Karpin-Galicia teams, refused to give the court permission to analyze his DNA.

''No, I don't want to,'' he said. ''I don't see why I should have to.''

Joseba Beloki, a 39-year-old former racer with ONCE and Liberty, also declined, saying he would have to think about it.

The riders were among four former Spanish professionals giving evidence under cross-examination on Tuesday.

Only Nozal acknowledged ever having received treatment from Eufemiano Fuentes, the doctor at the center of the Puerto case.

Fuentes is the key figure and one of five defendants in the case, which stems from a police raid in 2006 that uncovered blood bags and other doping equipment and implicated many of the world's top cyclists.

No riders are on trial because doping was not illegal in Spain at the time and the court is limited legally to trying Fuentes and his co-defendants, which include his sister Yolanda, of endangering public health.

Beloki said he was willingly collaborating with the case, although he had been subpoenaed to appear as a witness. He has denied any links with Fuentes.

''But if you ask me if I give my permission to have the blood bags analyzed, I would have to think about it,'' he told the court via a video link.

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2 former cyclists deny Puerto court DNA access

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DNA shows N.Z. girl not Madeleine McCann: police

Posted: at 11:44 am

DNA from a New Zealand girl bearing a striking resemblance to Madeleine McCann confirms she is not the child who disappeared in 2007, police said.

British authorities asked for the sample after the latest reported sighting of McCann in New Zealand, nearly six years after she went missing while holidaying with her family in Portugal at the age of three.

New Zealand police said they had been contacted twice by people convinced the child was McCann, first in March last year and again on New Year's Eve, when she was spotted in the southern resort town of Queenstown.

They investigated the first sighting and were "absolutely satisfied" it was not the missing girl but obtained a DNA sample after a request from Operation Grange, the British police task force investigating the disappearance.

Police said the New Zealand child's family provided the sample voluntarily and the DNA "does not provide a match for that of the missing girl".

"Given that there is conclusive evidence that their daughter is not Madeleine McCann and has no connection to Madeleine McCann, it is only right that she and her family are entitled to a level of privacy that most of us enjoy," they said.

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DNA shows N.Z. girl not Madeleine McCann: police

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3 'Bigfoot' genomes sequenced in 5-year DNA study

Posted: at 11:44 am

Public release date: 13-Feb-2013 [ | E-mail | Share ]

Contact: Robin Lynne media@sasquatchgenomeproject.org 231-622-5362 DNA Diagnostics

Dallas, Feb. 13--The multidisciplinary team of scientists, who on November 24, 2012 announced the results of their five-year long study of DNA samples from a novel hominin species, commonly known as "Bigfoot" or "Sasquatch," publishes their peer-reviewed findings today in the DeNovo Journal of Science (http://www.denovojournal.com). The study, which sequenced three whole Sasquatch nuclear genomes, shows that the legendary Sasquatch is extant in North America and is a human relative that arose approximately 13,000 years ago and is hypothesized to be a hybrid cross of modern Homo sapiens with a novel primate species. A species name, Homo sapiens cognatus, has been applied for through ZooBank. "Cognatus," from the Latin "con" (with) and "natus" (born), means "blood relative."

The study, "Novel North American Hominins, Next Generation Sequencing of Three Whole Genomes and Associated Studies," was conducted by a team of experts in genetics, forensics, imaging and pathology. The team, led by Dr. Melba S. Ketchum of DNA Diagnostics in Nacogdoches, TX, included Dr. Pat Wojtkiecicz, Director of the North Louisiana Criminalistics Laboratory; Ms. Aliece Watts of Integrated Forensic Laboratories in Euless, TX; Mr. David Spence, Trace Evidence Supervisor at Southwestern Institute of Forensic Sciences; Dr. Andreas K. Holzenburg, Director of the Microscopy & Imaging Center at Texas A&M University; Dr. Douglas G. Toler of Huguley Pathology Consultants in Fort Worth, TX; Dr. Thomas M. Prychitko of Helix Biological Laboratory in Michigan; Dr. Fan Zhang of the University of North Texas Health Science Center; and Sarah Bollinger, Ray Shoulders, and Ryan Smith of DNA Diagnostics.

In total, 111 specimens of purported Sasquatch hair, blood, skin, and other tissue types were analyzed for the study. Samples were submitted by individuals and groups at 34 different hominin research sites in 14 U.S. states and two Canadian provinces. Ketchum's team sequenced 20 whole and 10 partial mitochondrial genomes, as well as 3 whole nuclear genomes, from the samples.

Mitochondrial DNA (mtDNA) comes from mitochondria, energy-producing organelles in the cellular cytoplasm, and is passed down on the maternal lineage across generations. Nuclear DNA (nuDNA) is the genetic information contained in the cell nucleus and is the equal combination of DNA from the parents of an individual.

Initially a skeptic, Ketchum implemented strict protocols to ensure the scientific integrity of the study. DNA was extracted from samples using forensic procedures followed by screening of the samples to rule out contamination. Subsequently, the Q30 quality scores for all three genomes sequenced using the Illumina HiSeq 2000 platform were well above the average Q30 score of 85, indicating that the DNA used in the next generation sequencing was highly purified and originated from a single source for each of the three genomes sequenced. Prior to DNA testing, forensics experts examined the morphology of submitted hair samples against known human and animal samples.

"We soon discovered that certain hair samples--which we would later identify as purported Sasquatch samples--had unique morphology distinguishing them from typical human and animal samples," says Ketchum. "Those hair samples that could not be identified as known animal or human were subsequently screened using DNA testing, beginning with sequencing of mitochondrial DNA followed by sequencing nuclear DNA to determine where these individuals fit in the 'tree of life.'"

After extensive forensic controls to prevent contamination, mtDNA testing of the Sasquatch samples yielded fully modern human profiles. Sixteen haplotypes indicating 100% homology with modern human mtDNA sequences were observed from 20 completed whole and 10 partial mitochondrial genomes. The human mtDNA results are consistent with previous, unrelated mtDNA tests of purported Sasquatch samples from other laboratories.

Next-generation whole genome sequencing with the HiSeq 2000 platform by Illumina was performed at the University of Texas, Southwestern on one tissue sample, a saliva sample and one blood sample to produce 3 whole genomes. In contrast to the mtDNA which was unambiguously modern human, the Sasquatch nuDNA results were a mosaic of novel primate and human sequence.

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3 'Bigfoot' genomes sequenced in 5-year DNA study

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Applied DNA Sciences to Host Conference Call Friday, February 15, 2013

Posted: at 11:44 am

STONY BROOK, NY--(Marketwire - Feb 12, 2013) - Applied DNA Sciences, Inc. ( OTCBB : APDN ), aprovider of DNA-basedsecurity and anti-counterfeiting technology and product authentication solutions, will host a conference call on Friday, February 15, 2013 to discuss its earnings.

Applied DNA Sciences, Inc. management will host a thirty-minute conference call beginning at 9:00 a.m. eastern standard time on Friday, February 15, 2013 to discuss the company's performance and outline key ongoing and future business initiatives.A presentation by CEO and President James Hayward will be followed by a question-and-answer session.

All participants must pre-register using the link below. It is suggested that you log into the conference call approximately 10 minutes prior to the scheduled start time to ensure that all participants are on-line at the start of the call.While callers cannot be heard during the call, questions may be emailed beforehand to investor@adnas.com.

For those of you unable to participate, a transcript of the call will be posted by end of business on February 15, and available under the "Investors" tab of the company's web site.

To Participate: Reserve your spot now at: https://www3.gotomeeting.com/register/176683054

To Send Questions Prior to the Call: Please email questions prior to the call to investor@adnas.com.

Due to the large number of expected participants, not all questions may be answered on the call.

Company Background Materials: You may be interested in the company's Investor Information section on the company's web site, including publications, recent press releases, blogs, and other materials.

About APDNAPDN is a provider of botanical-DNA based security and authentication solutions that can help protect products, brands and intellectual property of companies, governments and consumers from theft, counterfeiting, fraud and diversion. SigNature DNA and BioMaterial Genotyping, our principal anti-counterfeiting and product authentication solutions that essentially cannot be copied, provide a forensic chain of evidence and can be used to prosecute perpetrators.

The statements made by APDN may be forward-looking in nature. Forward-looking statements describe APDN's future plans, projections, strategies and expectations, and are based on assumptions and involve a number of risks and uncertainties, many of which are beyond the control of APDN. Actual results could differ materially from those projected due to our short operating history, limited financial resources, limited market acceptance, market competition and various other factors detailed from time to time in APDN's SEC reports and filings, including our Annual Report on Form 10-K, filed on December 20, 2012 and our subsequent quarterly reports on Form 10-Q. APDN undertakes no obligation to update publicly any forward-looking statements to reflect new information, events or circumstances after the date hereof to reflect the occurrence of unanticipated events.

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Applied DNA Sciences to Host Conference Call Friday, February 15, 2013

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Girl not Madeleine McCann: DNA test crushes hopes lost girl had been found

Posted: at 11:44 am

DNA samples sent to Scotland Yard prove a young New Zealand girl is not the missing Madeleine McCann, who disappeared from a vacation apartment in Portugal in 2007.

Left: Madeleine before her disappearance. Right: A digitally-altered photo showing what Madeleine might look like today. (Metropolitan police)

Published: Feb. 13, 2013 at 9:57 AM

DNA results prove a young New Zealand girl is not Madeleine McCann, the British girl missing since May 2007.

The girl's family voluntarily submitted a DNA sample to police after she was spotted several times in Queenstown, New Zealand, in March and then again New Years Eve by a retailer who became suspicious by the resemblance she bore to McCann.

Police sent the sample to Scotland Yard, which confirmed that the DNA did not match that of the missing girl, New Zealand media reported.

"We have received confirmation from Scotland Yard that a DNA sample provided voluntarily from a girl mistaken as missing British girl Madeleine McCann on New Year's Eve in Queenstown last year does not provide a match for that of the missing girl," said Detective Inspector Steve McGregor of the New Zealand southern police.

"At the time of the sighting police made thorough inquiries and were completely satisfied that the girl was not Madeleine McCann."

The family of the Queenstown girl has requested to remain anonymous.

McCann was just shy of her fourth birthday when she disappeared while on vacation with her parents at the Ocean Club in Praia da Luz, Portugal on May 3, 2007.

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Girl not Madeleine McCann: DNA test crushes hopes lost girl had been found

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Christian Music Genome – Video

Posted: at 11:44 am


Christian Music Genome

By: Mary3859234

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Christian Music Genome - Video

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Restriction enzyme display in the UCSC Genome Browser – Video

Posted: at 11:44 am


Restriction enzyme display in the UCSC Genome Browser
This tip features a quick look at how to display restriction enzyme cut sites on the UCSC Genome Browser. For more details and references, visit: blog.openhelix.eu .

By: OpenHelixLLC

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Restriction enzyme display in the UCSC Genome Browser - Video

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Researchers Sequence Sasquatch Genome, Novel Hominins Extant in North America

Posted: at 11:44 am

Next-Generation Whole Genome Sequencing Proves Extant Human Relative, Findings Published in Denovo Journal of Science

(PRWEB) February 13, 2013

The team, led by Dr. Melba S. Ketchum, DVM, of DNA Diagnostics in Nacogdoches, TX, sequenced the three whole nuclear genomes using the next-generation Illumina HiSeq 2000 platform at University of Texas, Southwestern from a tissue sample, a saliva sample, and a blood sample. The three genomes all attained Q30 quality scores above 88 on the Illumina platform, significantly higher than the platform average of 85, indicating highly-purified, single-source DNA with no contamination for each sample. The three Sasquatch genomes align well with one-another and show substantial homology to primate sequences.

In addition to the three nuclear genomes, Ketchums team also sequenced 20 whole and 10 partial mitochondrial genomes from the Sasquatch samples. In contrast to the nuDNA results, the Sasquatch mtDNA was fully modern Homo sapiens, indicating that the species is a hybrid cross between modern Homo sapiens in the maternal lineage and an unknown hominin male progenitor.

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Novel North American Hominins, Next Generation Sequencing of Three Whole Genomes and Associated Studies.

Authors: Ketchum MS, Wojtkiewicz PW, Watts AB, Spence DW, Holzenburg AK, Toler DG, Prychitko TM, Zhang F, Bollinger S, Shoulders R, Smith R.

DeNovo. 13 February 2013.

Specimens yielding DNA were obtained, purportedly from elusive hominins in North America called Sasquatch. Sequencing and genotyping were performed in addition to histopathologic and electron microscopic examination of a large tissue sample.

Mitochondrial whole genomes were consistent with modern humans. In contrast, novel data were obtained when nuclear DNA was sequenced. Next generation whole genome sequencing was performed on three samples. Phylogeny trees generated showed homology to human chromosome 11 and to primate sequences. The data indicates that the Sasquatch has human mitochondrial DNA but possesses nuclear DNA that is a structural mosaic consisting of human and novel non-human DNA.

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Researchers Sequence Sasquatch Genome, Novel Hominins Extant in North America

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Whole genome sequencing better at tracing TB outbreaks than standard test

Posted: at 11:44 am

Public release date: 12-Feb-2013 [ | E-mail | Share ]

Contact: Sumrina Yousufzai syousufzai@plos.org 415-568-3164 Public Library of Science

A new form of genetic testing of the bacteria that causes tuberculosis can provide better information on TB transmission and also trace TB outbreaks more accurately than the current standard test, according to a study from Germany published in this week's PLOS Medicine.

A team of researchers led by Stefan Niemann from Forschungszentrum Borstel, Molecular Mycobacteriology, compared the results of the two types of tests on 86 M. tuberculosis isolates from a TB outbreak in the German states of Hamburg and Schleswig-Holstein between 1997 and 2010, in which 2301 people were diseased in the study period.

They found that the new test (whole genome sequencing) provided more accurate information on clustering and longitudinal spread of the pathogen than the standard test (classical genotyping). Importantly, whole genome sequencing revealed that first outbreak isolates were falsely clustered by classical genotyping and do not belong to one recent transmission chain.

By using whole genome sequencing, the authors estimated that the genetic material of M. tuberculosis evolved at a rate at 0.4 mutations per genome per year, suggesting that the bacterium grows in its natural host (infected people) with a doubling time of 22 hours, or 400 generations per year. This finding about the evolution of M. tuberculosis indicates how information from whole genome sequencing can be used to help trace future outbreaks.

Importantly, as the costs of whole genome sequencing are declining, this test could soon become the standard method for identifying transmission patterns and rates of infectious disease outbreaks.

The authors say: "Our study demonstrates that whole genome sequencing-based typing provides epidemiologically relevant resolution of large, longitudinal [Mycobacterium tuberculosis] outbreaks much more efficiently than classical genotyping."

They continue: "We envision that [whole genome sequencing] progressive effective implementation will be accelerated by the continuously decreasing sequencing costs, broader distribution of so-called bench top genome sequencers, and upcoming bioinformatics developments to facilitate quick and relevant interpretation of the resulting data in public health and medical contexts."

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Whole genome sequencing better at tracing TB outbreaks than standard test

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New York Genome Institute Selects Exemplar LIMS to Manage Next Gen Sequencing Labs

Posted: at 11:44 am

BALTIMORE, Feb. 12, 2013 /PRNewswire/ --Exemplar LIMS has been selected as the primary lab management and electronic lab notebook (ELN) solution for NY Genome Center's laboratory operations. Sapio's LIMS system will initially track next generation sequencing workflows from the Illumina family of sequencers from request through analysis. Support for additional next gen sequencers will be added as needed.

Sapio's LIMS software will be deployed on Apple iPads using Exemplar LIMS for Tablets solution. This enables lab technicians to take the LIMS with them as they move around the laboratory, improving LIMS usability and lab technician efficiency. NY Genome Center will also be utilizing Exemplar's Materials Management capabilities for detailed tracking of reagent usage. NYGC will ultimately be processing samples for both research and clinical applications via a CLIA certified laboratory. Sapio's experience with implementing best practices for both research and clinical labs will accelerate the LIMS implementation in support of these NYGC objectives.

"The team at New York Genome Center has been impressed with the flexibility and scalability of Sapio's offerings," commented NYGC President & Scientific Director Robert B. Darnell, MD, PhD. "With Sapio we found an entrepreneurial partner that understands how important customer service and collaboration are to NYGC. We look forward to working with Sapio to design a solution for the genome center of the future."

"The leadership at NYGC has recognized the importance of next generation sequencing technology for both research and clinical applications and has assembled a top-notch team from top to bottom. We are both honored and enthused to be supporting the NYGC objectives with the ultimate goal of curing diseases," said Kevin Cramer, VP at Sapio Sciences. "We look forward to working with the NYGC team towards creating a world-class lab operation as they scale to be one of the largest NGS facilities in the North America."

For more information or to schedule a product demonstration, visit http://www.SapioSciences.com.

About Sapio Sciences Llc

Sapio Sciences is a leading provider of LIMS and Data Management software, enabling scientists to manage samples, protocols, assay data and clinical trials.With extensive expertise in genomics/NGS labs, Exemplar LIMS breaks new ground in its breadth of functionality and ease of configuration. With built-in Sample Management, Electronic Lab Notebook (ELN), scientific data management (SDMS) and integrated analytics, Exemplar LIMS addresses the full life cycle of samples from receipt to processing and analysis. Sapio will continue to lead in ease of use and breadth of functionality across its product line, enablingorganizations toreduce costs while increasing productivity and efficiencyof both laboratories and researchers alike.

About New York Genome Center

Founded in August 2010, the New York Genome Center (NYGC) is an independent, non-profit organization leveraging the collaborative resources of leading academic medical centers, research universities, and commercial organizations. Our vision is to transform medical research and clinical care in New York through the creation of one of the largest genomics research facilities in North America, integrating sequencing, bioinformatics, and data management. For more information, visit http://www.nygenome.org

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