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NASA Quest: Possibility of colonizing Mars
Posted: December 23, 2013 at 5:45 am
QUESTION: Is it possible to colonize mars to the extent that human beings could live there? If so, then how would you go about it? Would we ever need to colonize Mars? ANSWER from R. Bourke on January 9, 1997: It may be possible for people to live on Mars in the future, but they must be protected from the environment. Conditions on Mars are much more harsh than any on Earth. It is very cold--far below freezing--there is no water, and the air is very thin and mostly carbon dioxide. Thus people will not be able to breath the air without space suits. In fact, machinery must be used to decompose the carbon dioxide to separate the oxygen to produce breathable air. ANSWER from Jack Farmer on January 30, 1997: The colonization of Mars by humans is being thought of in terms of "terraforming", which simply means modifying the Martian atmosphere and climate to make the "red planet" habitable for life. The Surface Environment of Mars: Cold, Dry and Full of Radiation The present surface of Mars is an inhospitable place for any terrestrial life form, whether microbe or person. The atmospheric pressure averages about 7.5 millibars (the avg. for Earth is 1000 mb). This means that water is unstable and can exist only as ice or vapor at the surface. In addition, the thin atmosphere of Mars is composed almost entirely of carbon dioxide (95%) and it lacks the oxygen needed to support human colonists without creating airtight shelters or spacesuits filled with our kind of atmosphere. The lack of oxygen also poses another risk. There is no protective ozone shield and the surface gets a very heavy dose of ultraviolet radiation (3-4X that at the Earth's surface). We know that UV is harmful to most life forms (that's why we have to use sunscreen!). As far as we know, Mars lacks a magnetic field, and that means that lots of other harmful radiation reaches the surface of Mars from the sun that, on Earth, is deflected away from the surface by the magnetic field. The temperature of the Martian surface is below freezing most of the time over most of the surface. Lastly, Mars is very cold (mostly below freezing most everywhere), but humans are able to live in cold climates on Earth, so that is not an insurmountable problem. However, combined with everything else, it makes colonization a real challenge. Building a Martian Atmosphere: An Oxygen-rich Greenhouse Terraforming would involve increasing the atmospheric density of Mars by liberating the water and carbon dioxide that lies frozen in the soils and on the polar caps of Mars to create a "greenhouse" effect (carbon dioxide and water both absorb heat energy from the sun and retain it causing the atmosphere to warm up) that would raise the surface temperature to the point where liquid water would be stable. There are a number of ideas for how to do that, but most involve installing large atmosphere-producing plants on Mars that would pump out carbon dioxide and perhaps other greenhouse gases like methane continuously until the atmiosphere was built up to the right level. Obviously, at some point we would also want to add oxygen to make the atmosphere breathable. On Earth, oxygen in our atmosphere is almost entirely formed by photosynthesis of green plants. So during terraforming, sometime after water ahd been rendered stable by the increased atmosphere, we would introduce photosynthesizing plants to contribute oxygen. What other benefit do you think oxygen would add, based on the preceeding discussion? There are a number of people and approaches that have been suggested for terraforming. Additional Reading: Some of these ideas were reviewed in a recent book by Robert Zubrin titled "The Case for Mars: The Plan to Settle the Red Planet and Why We Must" (1996, Free Press Publishing). Jack D. Farmer, PhD. NASA Ames Research Center ANSWER from Mark Adler on June 20, 1997: Yes. But it won't be easy. Mars is deathly cold (around minus 100 F), has a very thin carbon dioxide atmosphere with almost no oxygen, and has no ready supply of water. You'd have to *really* want to live there. A few people could be sustained on Mars with a steady line of supplies coming from Earth. In the long term, decades, we could find ways to provide some of those supplies on Mars, allowing the small colony to become more and more self-sufficient over time, and to possibly even grow. It would be a very long time, perhaps a century or more, before a colony on Mars could be completely independent. On even longer time scales, a few centuries, some people believe that we could change Mars globally to make it more hospitable for people by somehow making the atmosphere thicker and warmer. ANSWER from Mark Adler on July 29, 1997: There is certainly the possibility of humans exploring Mars. It's simply a matter of the rather high cost. Mars' atmosphere is extremely thin (less than 1/100th as thick as our atmosphere), is composed mostly of carbon dioxide, and is very cold. In short, Mars' atmosphere cannot support humans or other Earth life. However, that does not mean that we cannot inhabit the planet someday. We would need special enclosures to contain a warm, Earth-like atmosphere to live in. Mars' atmosphere of carbon dioxide and some nitrogen can be used, with some machinery and electrical power, to create and replenish an oxygen/nitrogen atmosphere in the enclosures. Water is a bit more of a problem, but there may be solutions there as well. Some people talk about actually changing Mars' atmosphere on a global scale to make it thicker and warmer in order to make habitation easier This is inspired in part by human's inadvertent but noticeable effect on Earth's atmosphere. However, it will likely be a long time before we have the technology and sheer presence to make global changes on Mars. Mark Adler ANSWER from Jim Murphy on June 22, 1998: I do not think that humans will NEED to colonize Mars, but I believe that we will have a very strong desire to do so since it is a challenge to be met (like going to the moon was..). I don't believe that current and future environmental problems we must deal with here on Earth are a good argument for developing a Mars colony. Rather, I'd prefer that we deal with our problems here. If we can develop the technology to build and sustain a Mars colony, then we can certainly develop strategies to deal with our problems here on earth without running (or rocketing) away to another planet. I certainly think that a Mars colony is possible. I would not expect one to be developed/built prior to 50-100 years from now, unless some form of life is discovered there, which would greatly accelerate the desire to get a base established. The cost will be enormous, and currently neither the money nor political will exists to fund the effort, but there are people thinking about how to do it. The advantages to having a Mars colony are to be able to conduct long-term science studies there, and to be able to travel over large distances on the surface repeatedly. Since the day length on Mars is 24 hours and 36 minutes, I would think it would be no problem to adjust to that day length. A colony would need to be "self contained", that is a structure not directly opened to the Martian atmosphere, since the Martian atmosphere is not capable of sustaining people (the lack of oxygen, and low atmospheric pressures would be deadly to anyone directly exposed to the atmosphere). Weather would play a role in determining how people travel about on the surface. Being out during a dust storm might make determining which direction to go difficult, and the large winds which might sometime blow could be a problem for some of the structure. ANSWER from Donna Shirley on July 9, 1998: I don't think humans "need" to colonize Mars in the sense that colonizing Mars will save us from the affects of overpopulating or despoiling the earth. If we get into such a situation I don't think that we will be able to afford to colonize a planet so far away. I think we "need" to explore Mars from the standpoint that we are a curious, exploring species. Donna Shirley
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NASA Quest: Possibility of colonizing Mars
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Moon in fiction – Wikipedia, the free encyclopedia
Posted: at 5:44 am
This article is about the Moon as the subject of and inspiration for creative works. For the Moon in mythology and religion, see Moon (mythology).
The Moon has been the subject of many works of art and literature and the inspiration for countless others. It is a motif in the visual arts, the performing arts, poetry, prose and music.
Lucian's Icaromenippus and True History, written in the 2nd century AD, deal with imaginary voyages to the moon such as on a fountain after going past the Pillars of Hercules. The theme did not become popular until the 17th century, however, when the invention of the telescope hastened the popular acceptance of the concept of "a world in the Moon", that is, that the Moon was an inhabitable planet, which might be reached via some sort of arial carriage. The concept of another world, close to our own and capable of looking down at it from a distance, provided ample scope for satirical comments on the manners of the Earthly world. Among the early stories dealing with this concept are:
The first flight to the Moon was a popular topic of science fiction before the actual landing in 1969.
Robert A. Heinlein wrote extensively, prolifically, and inter-connectedly about first voyages and colonization of the Moon, which he most often called Luna.[3] He also was involved with the films Destination Moon and Project Moonbase.
The Moon is sometimes imagined as having, now or in the distant past, indigenous life and civilization.
Human settlements on the Moon are found in many science fiction novels, short stories and films. Not all have the Moon colony itself as central to the plot.
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Moonbase Lunar Colony Simulator – Colonization
Posted: at 5:44 am
Title Moonbase, Lunar Colony SimulatorGame Type Management SimCompany Wesson InternationalPlayers 1
MAJOR MS-DOS PROBLEM - The PC Version of the program (below) won't run under later versions of MS-DOS (Microsoft Disk Operation System, with the 'C:>' Command Prompt) i.e. Windows XP, Vista Basic, Windows 7... Probably works on Windows 95/98. Will it work on Windows 8 (in production)??? It is run using the MS-DOS Shell, not Windows. Other Software that won't run includes Star Trek: The Birth of the Federation. This comes with a good manual off Ebay. But Microprose's Master of Orion II is superior in every way! And WORKS!!! Take a look right down the whole page as there are some good great [science fiction] pictures, and it gives a good jist of how the game works.
[JUN06] "Coiled tubing units are so compact and have such great potential, the Mars Drilling Project is evaluating a coiled tubing unit to drill for water on Mars." It can rotate up to 90 degrees, and drill 400 feet/hour driven by Nitrogen. Reinaldo Latham, Coil Tubing Technology, Inc. (CTBG)
I thought I would include a review of this game, as it is a similar concept to colonization, and so might be of interest to some readers.
This is a very unusual game, quite apart from the run of the mill. It is one of my favourites. The game is not by a software company as such, but by a Construction Company, predicting a near future Moonbase, and showing how it can be technically, and more importantly economically feasible, the Programmers consulted NASA experts. The concept gels well, and seems plausible, but one decimal point in the wrong place for commodity prices, and it could all fall to bits. The game rightly predicted that there was a high change of finding water deposits on the Moon, in the form and crashed meteorites. Since the real life discovery plans for a Moonbase have been revealed, showing just how critical it is. In the game you don't allows find water, it is winnable without it - just! But a walk over with it, as it is needed for drinking, growing food (it is highly costly to ship in), and for making oxygen and rocket fuel (H2O -> Liquid Oxygen and Hydrogen).
Also mine Helium-3 for [as yet un-discovered] radiation free Fusion Reactors. The Russians plan a Moon mission in 2015 for this very purpose! Make high quality Semiconductors (better quality due to low gravity), Solar Panels, and Equipment for missions through out the Solar System. Low Gravity means material can be fired into orbit using a cheap Mag-Lev system, as opposed to the extoriantly high cost of lifting out of Earth Orbit. Also build Hotels for Ultra-Rich tourist, and get NASA grants, that dry up over the years or if war occurs on Earth. Stock Market Prices for all commodities.
You also get a big manual, which is a story, with technical and game information scattered through it, most novel (pardon the pun). The story involves, not surprisingly, the foundation of a lunar colony. With another Chinese colony on the other side of the moon (again very plausible in today's world). Among other thing they have to prevent a melt down of a fission reactor, see to a crashed lander, break a strike, and trade solar power with the Chinese, as one side of the moon is in darkness, when the other is light, with a lunar might lasting 14 earth days, this makes a lot of sense.
For once the blurb on the back cover, actually reflects the game, so I will include it:
You are Commander of Project Moonbase, NASA's long-range plan to Earth's Moon. From your multi-million dollar annual budgets, you must establish a base, then manage its growth into a full-fledged, independent colony. With savvy (and luck), you may create a self-sufficient city on the Moon, but not without adeptly handling the myriad leadership problems in the highly-charged political and harsh physical environment.
Explore and exploit the Moon's surface Explore the lunar surface for new mining sites. Process the raw materials you find into oxygen, water and helium-3, then use then internally or sell them to Mars missions. Build hotels for fat cats from Earth. Profits from ventures like these can free you from Earth's purse strings - you might even want to declare independence!
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Moonbase Lunar Colony Simulator - Colonization
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Genetic Engineering | The Earth Times | Encyclopaedia
Posted: at 5:44 am
Genetic engineering is a scientific development that involves the artificial manipulation of an organism's genes by using techniques such as molecular cloning and transformation in order to alter their nature and structure. Many of these transformations are achieved by manipulation of an organism's DNA, which effectively is the code inscribed in every cell to determine how it will function.
As with most scientific developments there are a number of arguments both for and against.
There has been a considerable amount of research into the genetic engineering of crops such as potatoes, tomatoes, soybean and rice, with the aim of obtaining new strains that have better nutritional qualities and better yields.
In a world where there is a continual need to produce more food; genetically engineered crops are being developed to grow on land that is currently not suitable for cultivation. By manipulating the genes in crops the aim is to improve their nutritional value, their rate of growth and their flavour.
Seeds can be engineered so that they are resistant to pests and can survive cultivation in relatively harsh climatic conditions. Biotechnology can also be used to slow down the process of food spoilage so that fruit and vegetables can have a longer shelf life.
Although on the face of it genetic engineering might appear to bring a number of very positive benefits, there is by no means a universal approval of this practice.
Greenpeace International is very firm in its opposition, pointing out that there is no adequate scientific understanding of the impact that genetically modified organisms might have on the world's environment and on human health.
Undesirable genetic mutations can lead to allergies in crops and critics believe that while genetic engineering might enhance taste and appearance of foodstuffs, it could also hamper the nutritional value. At the very least, in order to inform consumers, all foodstuffs or products that have been made from genetically modified food should be clearly labelled as such at point of sale.
Whole new substances such as proteins and other food nutrients can be produced as a result of genetic engineering. The genetic modification of foods can be used to increase their medicinal value, thus making available a range of homegrown medical vaccines.
Greenpeace maintains that commercial interests are the prime movers to introduce genetically modified organisms into the food chain and stresses that once these organisms have been released into the environment they cannot be recalled.
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Genetic Engineering – Biology Questions and Answers
Posted: at 5:44 am
Learn the Fundamentals of Biotechnology
1. What is biotechnology?
Biotechnology is the application of biological knowledge to obtain new techniques, materials and compounds of pharmaceutical, medical, agrarian, industrial and scientific use, i.e., of practical use.
The pioneer fields of biotechnology were agriculture and the food industry but nowadays many other practical fields use its techniques.
2. What is genetic engineering?
Genetic engineering is the use of genetic knowledge to artificially manipulate genes: It is one of the fields of biotechnology.
3. At the present level of the biotechnology what are the main techniques of genetic engineering?
The main techniques of genetic engineering today are: the recombinant DNA technology (also called genetic engineering itself) in which pieces of genes from an organism are inserted into the genetic material of another organism producing recombinant beings; the nucleus transplantation technology, popularly known as cloning, in which a nucleus of a cell is grafted into a enucleated egg cell of the same species to create a genetic copy of the donor (of the nucleus) individual; the technology of DNA amplification, or PCR (polymerase chain reaction), that allows millions replications of chosen fragments of a DNA molecule.
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Genetic Engineering - Biology Questions and Answers
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Genetic Engineering | Buzzle.com
Posted: at 5:44 am
Genetic Engineering is a very complex field where there is a direct manipulation of an organism's genes. It is also called recombinant DNA technology, which involves creating a DNA by bringing together DNA sequences which otherwise, normally would not be combined. Techniques like transformation and molecular cloning are used in genetic engineering to modify the structure and the characteristics of genes.
Interesting Examples of Genetic Engineering
Genetic engineering is the technique that gives the power to desirably manipulate the genome of an organism. This ability has been explored and experimented in several organisms, some of which have been commercialized whereas the...
Common Misconceptions in Genetics
In the mid-19th century, Gregor Mendel propagated his theories related to heredity. A lot of progress has been made in the field of genetics since then. However, even today, there exist a lot of misconceptions owing to incorrect...
Benefits of Genetic Engineering
Genetic engineering process manipulates the DNA sequence to create a new one. The write-up focuses on the various benefits of genetic engineering.
Genetic Engineering in Humans
With the advancements in the field of genetic engineering, science in the future may give us the power to genetically modify and create 'near perfect' life. Read this write-up to know more about genetic engineering in humans.
Pros and Cons of Genetic Engineering
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Human genetic variation – Wikipedia, the free encyclopedia
Posted: at 5:44 am
Human genetic variation is the genetic differences both within and among populations. There may be multiple variants of any given gene in the human population (genes), leading to polymorphism. Many genes are not polymorphic, meaning that only a single allele is present in the population: the gene is then said to be fixed.[1] On average, biochemically all humans are 99.9% similar to any other humans.[2]
No two humans are genetically identical. Even monozygotic twins, who develop from one zygote, have infrequent genetic differences due to mutations occurring during development and gene copy number variation.[3] Differences between individuals, even closely related individuals, are the key to techniques such as genetic fingerprinting. Alleles occur at different frequencies in different human populations, with populations that are more geographically and ancestrally remote tending to differ more.
Causes of differences between individuals include the exchange of genes during meiosis and various mutational events. There are at least two reasons why genetic variation exists between populations. Natural selection may confer an adaptive advantage to individuals in a specific environment if an allele provides a competitive advantage. Alleles under selection are likely to occur only in those geographic regions where they confer an advantage. The second main cause of genetic variation is due to the high degree of neutrality of most mutations. Most mutations do not appear to have any selective effect one way or the other on the organism. The main cause is genetic drift, this is the effect of random changes in the gene pool. In humans, founder effect and past small population size (increasing the likelihood of genetic drift) may have had an important influence in neutral differences between populations. The theory that humans recently migrated out of Africa supports this.
The study of human genetic variation has both evolutionary significance and medical applications. It can help scientists understand ancient human population migrations as well as how different human groups are biologically related to one another. For medicine, study of human genetic variation may be important because some disease-causing alleles occur more often in people from specific geographic regions. New findings show that each human has on average 60 new mutations compared to their parents.[4][5] Apart from mutations, many genes that may have aided humans in ancient times plague humans today. For example, it is suspected that genes that allow humans to more efficiently process food are those that make people susceptible to obesity and diabetes today.[6]
Genetic variation among humans occurs on many scales, from gross alterations in the human karyotype to single nucleotide changes.[7]
Nucleotide diversity is the average proportion of nucleotides that differ between two individuals. The human nucleotide diversity is estimated to be 0.1%[8] to 0.4% of base pairs.[9] A difference of 1 in 1,000 amounts to approximately 3 million nucleotide differences, because the human genome has about 3 billion nucleotides.
A single nucleotide polymorphism (SNP) is difference in a single nucleotide between members of one species that occurs in at least 1% of the population. It is estimated that there are 10 to 30 million SNPs in humans.
SNPs are the most common type of sequence variation, estimated to comprise 90% of all sequence variations.[10] Other sequence variations are single base exchanges, deletions and insertions.[10] SNPs occur on average about every 100 to 300 bases [10] and so are the major source of heterogeneity.
A functional, or non-synonymous, SNP is one that affects some factor such as gene splicing or messenger RNA, and so causes a phenotypic difference between members of the species. About 3% to 5% of human SNPs are functional (see International HapMap Project). Neutral, or synonymous SNPs are still useful as genetic markers in genome-wide association studies, because of their sheer number and the stable inheritance over generations.[10]
A coding SNP is one that occurs inside a gene. There are 105 Human Reference SNPs that result in premature stop codons in 103 genes. This corresponds to 0.5% of coding SNPs. They occur due to segmental duplication in the genome. These SNPs result in loss of protein, yet all these SNP alleles are common and are not purified in negative selection.[11]
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Human genetic variation - Wikipedia, the free encyclopedia
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Make a strawberry DNA cocktail – Video
Posted: at 5:44 am
Make a strawberry DNA cocktail
It #39;s mixology meets biology. Watch along as TED Fellow Oliver Medvedik walks you through the steps of how to create a yummy adult beverage that isolates stra...
By: TED Blog Video
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Make a strawberry DNA cocktail - Video
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DNA Evidence Proves That The First People In China Were Black
Posted: at 5:44 am
Details Published on Monday, 23 December 2013 14:40
The lower part of the face of the Emperor Pu-yi of Manchukuo, direct descendant of the Manchu rulers of China, is most distinctly Negroid/Kulture KriticCHINA is apparently finding out now what Black historians have been reporting for many years, the first inhabitants of China were in fact black.
H. Imbert, a French anthropologist said in his book, Les Negritos de la Chine,The Negroid races peopled at some time all the South of India, Indo-China and China. The South of Indo-China actually has now pure Negritos as the Semangs and mixed as the Malays and the Sakais.
Another author and professor, Chang Hsing-Lang, revealed similar information in writing The Importation of Negro Slaves to China under the Tang Dynasty, Even the sacred Manchu dynasty shows this Negro strain. The lower part of the face of the Emperor Pu-yi of Manchukuo, direct descendant of the Manchu rulers of China, is most distinctly Negroid.
These professors through their research and studies have reason to believe that a Negro Empire actually existed at the dawn of the countrys history citing evidence of substantial populations of Blacks in early China, including finding reports of a major kingdom ruled by Blacks being frequently mentioned in historical Chinese history documents. And,Chinese chroniclers report that a Negro Empire existed in the South of China at the dawn of that countrys history
The notion that blacks were the original inhabitants of China has been thwarted by white scientists and even some blacks as the result of a sweeping message of white superiority and inferiority of black Africans and their descendants spreading worldwide.
Negroid bone structure/Google ImagesIn 2005, DNA testing proved that the first inhabitants of China were black Africans. The study was conducted by a Chinese DNA specialist named Jin Li and a team of Chinese and other scientists. Li admits that he wasnt trying to prove this fact, instead he initially wanted to prove that the Chinese evolved from hmo erectus independently of all humans. After collecting more than 12000 DNA samples from 165 different ethnic groups, Li and his team found that early humans belonged to different species but modern humans had descended from the East African species.
One scientist on the team, Li Hui, said that 100,000 years ago humans began migrating through South and Southeast Asia into China from Africa. Their testing showed that 65 branches of Chinese all carry similar DNA mutations as the people of Southeast Asia.
Another scientist on the team, Jin Li had this to say about their findings, we did not see even one single individual that could be considered as a descendant of the hmo erectus in China, rather, everybody was a descendant of our ancestors from Africa.
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DNA Evidence Proves That The First People In China Were Black
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DNA profiling – Wikipedia, the free encyclopedia
Posted: at 5:44 am
DNA profiling (also called DNA testing, DNA typing, or genetic fingerprinting) is a technique employed by forensic scientists to assist in the identification of individuals by their respective DNA profiles. DNA profiles are encrypted sets of numbers that reflect a person's DNA makeup, which can also be used as the person's identifier. DNA profiling should not be confused with full genome sequencing.[1] It is used in, for example, parental testing and criminal investigation.
Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different to distinguish one individual from another, unless they are monozygotic twins.[2] DNA profiling uses repetitive ("repeat") sequences that are highly variable,[2] called variable number tandem repeats (VNTRs), particularly short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but so variable that unrelated individuals are extremely unlikely to have the same VNTRs.
The DNA profiling technique was first reported in 1984[3] by Sir Alec Jeffreys at the University of Leicester in England,[4] and is now the basis of several national DNA databases. Dr. Jeffreys's genetic fingerprinting was made commercially available in 1987, when a chemical company, Imperial Chemical Industries (ICI), started a blood-testing centre in England.[5]
The process begins with a sample of an individual's DNA (typically called a "reference sample"). The most desirable method of collecting a reference sample is the use of a buccal swab, as this reduces the possibility of contamination. When this is not available (e.g. because a court order may be needed and not obtainable) other methods may need to be used to collect a sample of blood, saliva, semen, or other appropriate fluid or tissue from personal items (e.g. toothbrush, razor, etc.) or from stored samples (e.g. banked sperm or biopsy tissue). Samples obtained from blood relatives (biological relative) can provide an indication of an individual's profile, as could human remains which had been previously profiled.
A reference sample is then analyzed to create the individual's DNA profile using one of a number of techniques, discussed below. The DNA profile is then compared against another sample to determine whether there is a genetic match.
The first methods for finding out genetics used for DNA profiling involved restriction enzyme digestion, followed by Southern blot analysis. Although polymorphisms can exist in the restriction enzyme cleavage sites, more commonly the enzymes and DNA probes were used to analyze VNTR loci. However, the Southern blot technique is laborious, and requires large amounts of undegraded sample DNA. Also, Karl Brown's original technique looked at many minisatellite loci at the same time, increasing the observed variability, but making it hard to discern individual alleles (and thereby precluding parental testing). These early techniques have been supplanted by PCR-based assays.
In 1985 (see Mullis and Faloona 1987) a process was reported by which specific portions of the sample DNA can be amplified almost indefinitely (Saiki et al. 1985, 1988). This has revolutionized the whole field of DNA study. The process, the polymerase chain reaction (PCR), mimics the biological process of DNA replication, but confines it to specific DNA sequences of interest.
In this process, the DNA sample is denatured into the separate individual strands. Two DNA primers are used to hybridize to two corresponding nearby sites on opposite DNA strands in such a fashion that the normal enzymatic extension of the active terminal of each primer (that is, the 3 end) leads toward the other primer. In this fashion, two new copies of the sequence of interest are generated.
Repeated denaturation, hybridization, and extension in this fashion produce an exponentially growing number of copies of the DNA of interest. The denaturation is generally performed by heating, and in this case using, replication enzymes that are tolerant of high temperatures (Taq DNA polymerase). Instruments that perform thermal cycling are now readily available from commercial sources. This process can produce a million-fold or greater amplification of the desired region in 2 hours or less.
With the invention of the polymerase chain reaction (PCR) technique, DNA profiling took huge strides forward in both discriminating power and the ability to recover information from very small (or degraded) starting samples. PCR greatly amplifies the amounts of a specific region of DNA, using oligonucleotide primers and a thermostable DNA polymerase. Early assays such as the HLA-DQ alpha reverse dot blot strips grew to be very popular due to their ease of use, and the speed with which a result could be obtained. However they were not as discriminating as RFLP. It was also difficult to determine a DNA profile for mixed samples, such as a vaginal swab from a sexual assault victim.
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DNA profiling - Wikipedia, the free encyclopedia
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