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UPPSALA, Sweden, May 09, 2022 (GLOBE NEWSWIRE) -- Olink Holding AB (publ) ( OLK) today announced that Oxford Genomics at the University of Oxford adopts the Olink technology and becomes the first Olink certified laboratory in the United Kingdom. The partnership will enable novel techniques to unravel mechanisms of disease using the Olink Explore platform.

Oxford Genomics is centered within the Wellcome Centre of Human Genetics which was formed in the founding years of the Human Genome Project; they have been producing cutting edge research for more than two decades. As we move into an age of multi-omic analysis to truly understand the linkage between disease and phenotype, proteomics is an essential tool to complement their other cutting-edge technologies.

With the recent establishment of the Oxford-GSK Institute, Olink Explore will be utilized to build a multiomics approach to mapping molecular mechanisms of complex diseases such as Parkinsons and Alzheimers. Expertise in machine learning and bioinformaticians at Oxfords Big Data Institute will be able to leverage these datasets to pinpoint novel targets and identify signatures to stratify patients.

By utilizing the Olink platform we are interested in discovering biomarkers and early disease signatures in common diseases, because they would provide clues to druggable targets and readouts we can use to test potential therapeutic candidates, said Prof John Todd Director of Wellcome Centre for Human Genetics and Co-Director of Oxford GSK Institute. We are trying to make the drug development process more precise by understanding the heterogeneity in the patients instead of one drug fits all.

The new Olink Explore 3072 platform enables access to an expanded library of carefully curated and validated assays to provide detailed proteomics data to improve understanding of human health. The Olink market-leading proteomics solution measures up to 3,000 proteins per sample using Proximity Extension Assay (PEA) technology combined with next generation (NGS) sequencing readout, providing a highly accurate and reproducible multiplexed method with exceptional specificity.

We are immensely proud to lay the foundation of a long lasting and prosperous partnership with such a prestigious institution as the University of Oxford, utilizing our technology as the first Olink certified laboratory in the United Kingdom. This partnership demonstrates the importance of academic partners in pioneering the establishment of new technologies. It will further democratize the use of the Olink platform in line with our mission to accelerate proteomics together with the scientific community, said Jon Heimer, CEO, Olink Proteomics. The objective is to create a better understanding of the origin of diseases, provide earlier and more accurate diagnoses with individualized treatment and enable more efficient and safer drug development.

Investor Contact Jan Medina, CFAVP Investor Relations & Capital MarketsMobile: +1 617 802 4157[emailprotected]

Media Contact Andrea PranderCorporate Communications Manager Mobile: +46 768 775 275[emailprotected]

About OlinkOlink Holding AB ( OLK) is a company dedicated to accelerating proteomics together with the scientific community, across multiple disease areas to enable new discoveries and improve the lives of patients. Olink provides a platform of products and services which are deployed across major pharmaceutical companies and leading clinical and academic institutions to deepen the understanding of real-time human biology and drive 21st century healthcare through actionable and impactful science. The company was founded in 2016 and is well established across Europe, North America and Asia. Olink is headquartered in Uppsala, Sweden.

About Wellcome Centre for Human GeneticsThe Wellcome Centre for Human Genetics (WCHG) is a research institute of the Nuffield Department of Medicine at the University of Oxford, funded by the University, Wellcome, and numerous other sponsors. It is based in purpose-built laboratories on the University of Oxfords Biomedical Research Campus in Headington, one of the largest concentrations of biomedical expertise in the world. Here our researchers are able to work closely with colleagues across University departments including, but not limited to, the Department of Psychiatry, the Division of Cardiovascular Medicine and theBig Data Institute.

With more than 400 active researchers and around 70 employed in administrative and support roles, the Centre is an international leader in genetics, genomics and structural biology. WCHG collaborates with research teams across the world on a number of large-scale studies in these areas. WCHGs researchers expend close to 20m annually in competitively-won grants and publish around 300 primary papers per year.https://www.well.ox.ac.uk/

About the University of OxfordOxford University has been placed number 1 in the Times Higher Education World University Rankings for the sixth year running, and number 2 in the QS World Rankings 2022. At the heart of this success are the twin-pillars of our ground-breaking research and innovation and our distinctive educational offer.

Oxford is world-famous for research and teaching excellence and home to some of the most talented people from across the globe. Our work helps the lives of millions, solving real-world problems through a huge network of partnerships and collaborations. The breadth and interdisciplinary nature of our research alongside our personalised approach to teaching sparks imaginative and inventive insights and solutions.

Through its research commercialisation arm, Oxford University Innovation, Oxford is the highest university patent filer in the UK and is ranked first in the UK for university spinouts, having created more than 200 new companies since 1988. Over a third of these companies have been created in the past three years. The university is a catalyst for prosperity in Oxfordshire and the United Kingdom, contributing 15.7 billion to the UK economy in 2018/19, and supports more than 28,000 full time jobs.

Forward-Looking StatementsThis release may contain forward-looking statements within the meaning of applicable securities laws, including the U.S. Private Securities Litigation Reform Act of 1995, as amended, including, without limitation, statements regarding Olinks strategy, business plans and focus. The words may, will, could, would, should, expect, plan, anticipate, intend, believe, estimate, predict, project, potential, continue, target and similar expressions are intended to identify forward-looking statements, although not all forward-looking statements contain these identifying words. Any forward-looking statements in this press release are based on managements current expectations and beliefs as of the date hereof and are subject to a number of risks, uncertainties and important factors that may cause actual events or results to differ materially from those expressed or implied by any forward-looking statements contained in this press release, including, without limitation, those related to Olinks business, operations, supply chain, strategy, goals and anticipated timelines, including for the delivery of Olink Explore 3072 and the expansion of the Explore platform, competition, and other risks identified in the section entitled Risk Factors in Olinks Registration Statement on Form F-1, as amended (File No. 333-253818) filed with the U.S. Securities and Exchange Commission (SEC) and in the other filings, reports, and documents Olink files with the SEC from time to time. Olink expressly disclaims any obligation to update any forward-looking statements in this release to reflect any change in its expectations with regard thereto or any change in events, conditions or circumstances on which any such statement is based, unless required by law or regulation.

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University of Oxford adopts Olink technology to advance protein biomarker discovery and unravel mechanisms of disease - GuruFocus.com

The American Society for Biochemistry and Molecular Biology announced today the winners of its annual awards. Colleagues and other leaders in the field nominated the winners for making significant contributions to biochemistry and molecular biology and to the training of emerging scientists.

The recipients will give talks about their work at the societys2023 annual meeting, Discover BMB, slated for March 2528 in Seattle.

In addition to cash prizes ranging from $500 to $35,000, each ASBMB award consists of a plaque and transportation expenses to the ASBMB annual meeting.

Learn more about the ASBMB awards.

Regina Stevens-Truss

Recognizes an individual who encourages effective teaching and learning of biochemistry and molecular biology.

Regina StevensTruss is a professor at Kalamazoo College in Michigan who has served in numerous leadership positions at the ASBMB. She has been a member of the societys Education and Professional Development Committee and Minority Affairs Committee (now Maximizing Access Committee). She is a past member of the steering committee that created the concept-driven teaching strategies that laid the foundation for the ASBMBs certification exam. She was the principal investigator in 2012 on a National Science Foundation grant that supported a STEM K-12 outreach initiative by the society called Hands-on Outreach to Promote Engagement in Science (HOPES for short).

Squire Booker

Recognizes outstanding contributions to research in biochemistry and molecular biology.

Squire J. Bookeris an Evan Pugh professor of chemistry and of biochemistry and molecular biology and the Eberly Family distinguished chair in science at The Pennsylvania State University. He is also an investigator of the Howard Hughes Medical Institute. His lab studies catalytic mechanisms of redox enzymes involved in natural product biosynthesis and human health. He is deputy editor of ACS Bio & Med Chem Au, an open-access journal of the American Chemical Society, and an executiveassociate editor of the ACS journal Biochemistry. He becamean inaugural fellowof the ASBMB in 2021. He also won this years Ruth Kirschstein Diversity in Science Award. (See below.)

Russell DeboseBoyd

Recognizes outstanding research contributions in the area of lipids.

Russell DeBoseBoydis the Beatrice and Miguel Elias distinguished chair in biomedical science and professor of molecular genetics at the University of Texas Southwestern Medical Center at Dallas. DeBoseBoyds lab studies regulatory mechanisms governing feedback regulation of HMG-CoA reductase, the rate-limiting enzyme in cholesterol synthesis. He is an associate editor for the Journal of Lipid Research and an editorial board member for the Journal of Biological Chemistry, both ASBMB journals. Readour Q&Awith DeBoseBoyd.

Erica Saphire

Awarded to an established scientist for outstanding accomplishments in basic biomedical research.

Erica Ollmann Saphire is a professor and the president and chief executive officer of the La Jolla Institute for Immunology. Saphires lab has solved structures of key proteins of the Ebola, Marburg, rabies and Lassa viruses and explained how they remodel these structures as they drive themselves into cells, how their proteins suppress immune function and where human antibodies can defeat these viruses. She used this information to galvanize two international consortia of former competitors to advance antibody therapeutics together. Saphire is a two-time ASBMB award winner. In 2015, she won the ASBMB Young Investigator Award.

Eytan Ruppin

Given to a scientist forthemost accessible and innovative development or application of computer technology to enhance researchin the life sciencesat the molecular level.

Eytan Ruppin is a computational biologist and chief of the Cancer and Data Science Laboratory in the Center for Cancer Research at the National Cancer Institute. His lab develops computational approaches for the integration of multiomics data to understand better the pathogenesis and treatment of cancer. His research focuses on basic and translational studies aimed at broadening the scope of precision oncology to the realm of tumor transcriptomics.

Scott Dixon

Awarded to a scientist with 10 years or less of post-postdoctoral experience.

Scott Dixonis an associate professor in the biology department at Stanford University.His labstudies cell death and lipid metabolism using small-molecule screening, biochemical analysis of protein function, and model organism genetics. Dixon is a member of theprogram planning committeefor Discover BMB, the societys annual meeting.

Anne Kenworthy

Recognizes and honors scientists at all stages of their careers who have made substantial advances in understanding biological chemistry using innovative physical approaches.

Anne Kenworthy is a professor of molecular physiology and biological physics at the University of Virginia and the assistant director of its Center for Membrane and Cell Physiology. Her lab studies membrane nanodomains, such as lipid rafts and caveolae, to learn how they assemble and function in health and disease. (Read about her recent high-content analysis of membrane vesicles.)Together with collaborators at the University of Michigan and Vanderbilt University, her group also recently provided the first glimpse into molecular architecture of an essential building block of caveolae oligomeric complexes formed by the membrane protein caveolin-1.

Squire Booker

Honors an outstanding scientist who has shown a strong commitment to the encouragement of scientists from historically marginalized groups.

This is the second award this year forSquire J. Booker, a professor and distinguished chair at The Pennsylvania State University. (See the ASBMBMerck Award above.) Booker is a past chair of the ASBMBs Minority Affairs Committee and established the ASBMBgrant-writing workshop, which now is known as the Interactive Mentoring Activities for Grantsmanship Enhancement workshop. He also co-organized the 2016 ASBMB annual meeting. He now serves on the Finance and Nominating committees.

Itay Budin

Recognizes outstanding research contributions in the area of lipids by a young investigator.

Itay Budin is an assistant professor of chemistry and biochemistry at the University of California San Diego. His laboratory uses approachesranging from membrane biophysics to synthetic biology to investigate lipid function. Current areas of focus in his lab include the inner mitochondrial membrane and lipid adaptation for life in extreme conditions. In 2017, Budin received a Journal of Biological Chemistry/Herbert Tabor Young Investigator Award.

Catherine Drennan

Recognizes outstanding contributions to biochemical and molecular biological research and a demonstrated commitment to the training of younger scientists.

Catherine Drennanis a professor at the Massachusetts Institute of Technology and a Howard Hughes Medical Institute investigator.Drennans labstudies the structural biology of metalloenzymes. Her teams targets have included multiple enzymes that depend on metal cofactors, such as ribonucleotide reductase, an early enzyme in DNA biosynthesis. She is a former member of the ASBMB Education and Professional Development Committee. As a postdoctoral fellow, she started the undergraduate poster competition at the ASBMBs annual meeting. Her pedagogical work includes research into best practices for active lectures and the development of resources that help undergraduates appreciate the value of chemical principles in biology and medicine. She was a member of the ASBMBsinaugural classof fellows in 2021.

Gira Bhabha

Recognizes individuals with a strong commitment to advancing the careers of women in biochemistry and molecular biology along with demonstrated excellence in research and/or service.

Gira Bhabhais an assistant professor at the NYU Grossman School of Medicine, where she began her independent career in 2017. The Bhabha lab works closely with the lab of Damian Ekiert; since their inception, the two labs have functioned synergistically as a single group. TheBhabha and Ekiert labsstudy structural mechanisms and cell biology of microbes and their interactions with hosts, using integrative approaches including X-ray crystallography, cryo-electron microscopy, cryo-electron tomography, optical microscopy, biochemistry, microbiology and cell biology techniques.

Kerry-Ann Rye

Recognizes individuals with a strong commitment to advancing the careers of women in biochemistry and molecular biology along with demonstrated excellence in research and/or service.

Kerry-Anne Rye is a professor at the University of New South Wales in Sydney and co-editor-in-chief of the ASBMBs Journal of Lipid Research. Before taking the helm at the JLR in 2020, she had been an associate editor since 2008. She has been a research professor since 2013 at UNSW, where she serves as the deputy head of the School of Medical Sciences and studies atherosclerosis and diabetes. Rye was a member of the inaugural class of ASBMB fellows in 2021. She wrote an essay earlier this year about being a member of the society.

Dyann Wirth

Keith Matthews

Recognizes established investigators who are making seminal contributions to the field of molecular parasitology.

Dyann Wirth is a professor at Harvard Universitys T.H. Chan School of Public Health and the Broad Institute. Her lab studies the Plasmodium genus, members of which commonly infect humans with malaria. Her team is working on methods for molecular genetic manipulation of protozoan parasites to analyze genes important for their virulence and resistance to drugs.

Keith Matthewsis a professor at the University of Edinburgh.His laboratorystudies African trypanosomes, parasites spread by the tsetse fly, and the changes they undergo in the fly, using targeted reverse genetic approaches, global RNA and protein analysis, and other strategies.

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ASBMB names 2023 award winners - ASBMB Today

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Strain-specific predation of Bdellovibrio bacteriovorus on Pseudomonas aeruginosa with a higher range for cystic fibrosis than for bacteremia isolates...

Advances in genetic technology have rapidly changed healthcare delivery in low- and middle-income countries. NGS utilization has decreased the time to diagnosis, increased the diagnostic rate, and provided valuable insight into the genotypephenotype correlation of IEI in a timely and cost-effective way28,29. IEI is not uncommon in India; however, their diagnosis is either missed or delayed due to a lack of awareness and a paucity of diagnostic facilities. There is an urgent need to increase testing capacity for early recognition, diagnosis, and management of IEI in our country30,31,32.

We have been diagnosing patients with IEI at our centre for the past 25years. However, services for molecular diagnosis for IEI both in government and commercial sectors have not been available in India until 2016. For molecular diagnosis of IEI, we established academic collaboration with Service Hmatologie, Immunologie et de Cytogntique, Hpital de Bictre, Le Kremlin Bictre, at France in the year 2007. Later, we established collaboration with institutes at Japan (National Defense Medical College, Saitama) and Hong Kong (Department of Paediatric and Adolescent Medicine, University of Hong Kong) in the years 2008 and 2010, respectively. This has facilitated molecular diagnosis for many of our patients with IEI. Our centre was designated as Centre for Advanced Research in diagnosis and treatment for primary immunodeficiency diseases by the Indian Council of Medical Research, Government of India, in 2015. Until 2016, tests available for diagnosis of IEI at our centre include immunoglobulin estimation, NBT, and flow cytometry for several surface and intracellular proteins10. With the increase in patients diagnosed with IEI in the last few years, we felt the need to establish molecular analysis at our centre4. We initiated Sanger sequencing for BTK, CYBB, and WAS genes in our centre in 2016 (Fig.1).

Commercial laboratories in India came up with facilities (targeted exome) for molecular diagnosis of IEI in 2016. Costs incurred for sequencing in commercial laboratories were exorbitant (USD 400500) in 2016 that later reduced in the subsequent years (USD 200 currently). The introduction of targeted NGS for IEI in 2018 at our centre has enabled us to offer this diagnostic modality to many of our patients who could not afford the costs of commercial testing. We have also been able to diagnose more IEIs each year and at a much faster pace than in previous years. The cost of targeted genetic sequencing at our setup is USD 83 per sample. This is much less than the costs incurred at commercial laboratories in India33. In addition, infants less than one year are covered under the JSSK (Janani Sishu Suraksha Karyakram) scheme of the Government of India. They are entitled to avail of NGS free of cost. Our Institute also provides free diagnostic services to patients from low-income groups who cannot afford the NGS charges, and charges are minimal for those who can afford this facility.

We have worked upon and improvised the standard protocol of NGS to suit our setup. We made some ingenious modifications to the recommended protocol to reduce the cost per sample and accommodate more patient samples in each run. Towards this end, we have successfully used half the recommended volume of reagents (however, concentration remained the same) at each successive step by starting with an initial DNA volume of 2.5L instead of 5L. So, a larger number of patient samples could be accommodated in each run. We have effectively run 42 patient samples with a 24-reaction reagent kit for 24 samples.

NGS sample preparation is a tedious and labour-intensive process requiring focus and concentration at each successive step34,35. After chip-loading and sequencing, we did not get results for two runs. On both these occasions, instead of repeating from the start, we started after the library quantification step as we were sure about the quality of the library preparation. So, restarting with the template preparation step instead of beginning from the start in the case of a failed run could be a helpful strategy if we are sure about the quality of library preparation.

We describe preliminary results of targeted NGS in 121 patients with different forms of IEIs diagnosed and managed at our centre. Our variant pick-up rate of 63.6% is much higher than previous studies- 25% by Yska et al. in 2019 and 29% by Vorsteveld et al. in 202128,36. The pick-up rate of variants in other studies were 16%7 (Gallo et al., Italy, 2016), 14% (Kojima et al., Japan, 2016)37, 2.1% (Sun et al., China in a cohort of infants)38, 28.6% (Cifaldi et al., Italy, 2020)18 and 42.4% (Arunachalam et al., India, 2020)33.

There are several reasons for a higher diagnostic yield in our study. Careful patient selection with a high pre-test probability based on clinical manifestations and preliminary immunological investigations was done. Patients with a high likelihood of having a pathogenic variant in one of 44 genes included in the gene panel are sorted out in consultation with clinicians trained in immunology and have broad experience in caring and managing patients with IEI. Currently more than 400 genes are implicated in various IEI. However, we selected 44 genes based on the most common diseases we encounter at our centre and also since we aimed to provide genetic diagnosis to maximum number of patients at an affordable cost. A large panel although more desirable would be costlier to design and in addition fewer samples would be accommodated in each run. Samples of patients who are very likely to have genetic variants in the genes included in the panel were included based on clinical history and initial immunological investigations. Patients with IEI not clearly delineated upon initial immunological investigations are referred for a clinical exome or whole-exome analysis. This analysis is outsourced to commercial laboratories providing these services at an affordable cost.

NGS has facilitated the early diagnosis of patients with IEI in situations where flow cytometry was either not conclusive or did not match the clinical presentation. For instance, patient 56 was clinically suspected of having an autosomal recessive hyper-IgM was found to have biallelic variants in the ATM gene. Hence, relying solely on typical manifestations of the IEI may not be ideal, and a rapid genetic diagnosis is indispensable39.

There have also been instances when the initial analysis on the Ion Reporter did not reveal a pathogenic variant. In patient 8 with clinically suspected XLA, no pathogenic variant was detected at initial analysis. There was a strong clinical suspicion of XLA in this case; we manually visualized the data on Integrative Genomics Viewer (IGV). We found a large deletion of exon-10, 11 and 12 in the BTK gene (Fig.2)40. Similarly, in another patient with suspected CGD (Pt.27), a large deletion was found in the CYBA gene, which was missed by the ion reporter software but was detected on manual reanalysis and visualization on the IGV. Patient 42 had an indel in IL2RG gene. In patient 42, analysis by the Ion reporter software revealed two IL2RG variants in close proximity, which was confusing. However, upon visualization of the BAM file on IGV, we realized that it was an indel (insertion of 3 nucleotides and deletion of 8 nucleotides) which was misinterpreted as two variants by the ion Reporter software.

Large deletion of Exon- 10 to 12 in BTK gene on Integrative Genome Viewer.

Hence, manual data visualization on IGV and manual analysis of annotated vcf files instead of relying on variants detected by initial analysis by software is crucial. We have been able to detect these variants in these cases using this strategy.

Detection of genetic variants in genes with known pseudogene is another problem that we encountered in our patient cohort. We faced this difficulty in patients with autosomal recessive CGD due to NCF1 gene defect. The targeted NGS panel systematically missed the most common pathogenic variant in NCF1, i.e., deletion of two nucleotides at the start of Exon-2. NCF1 gene has two flanking pseudogenes (NCF1)41. We assume that the amplicon designed for exon-2 of the NCF1 gene was unable to bind to its target, and thus, there was no amplification of this region, resulting in no reads for exon-2 in these patients. We performed a gene scan in 3 patients who had no reads in Exon-2 of the NCF1 gene to check for this variant and confirmed NCF1 GT deletion in all 3 of these patients (Fig.3A,B).

(A) IGV snapshot showing no reads from Exon-2 of NCF1 gene in 2 patients with AR-CGD (B) Gene Scan for Exon 2 NCF1 gene from control and an AR-CGD patient with no reads from exon 2 of NCF1 gene.

We have also been able to offer prenatal services to many patients. Patient 40 was clinically suspected of having SCID but had expired before a genetic defect could be established. His mother was pregnant at this time, and the period of gestation was 13weeks. We were able to identify a splice-site variant in the IL2RG gene in this family with X-linked SCID, and the mother was offered prenatal diagnosis by chorionic villous sampling. Molecular confirmation of diagnosis helped the family to get timely antenatal testing and appropriate genetic counselling. For some patients, especially SCID, rapid diagnosis through targeted NGS has saved lives, or genetic counselling has prevented an affected child in the subsequent pregnancy.

Pt 76 was the mother of a deceased child suspected to have X-linked Hyper-IgM, but a genetic diagnosis could not be established during the childs life. Targeted NGS revealed a synonymous variant in exon 1 of the CD40LG gene proximal to donor splice-site. In-silico prediction for this variant was found to be damaging by Mutation Taster2. Synonymous variants involving canonical splice-sites can also be pathogenic and should not be filtered out.

Genetic findings were beneficial in providing genetic counselling to affected families, carrier screening, and prenatal diagnosis. Moreover, genetic information is required for devising appropriate transplantation related strategies. Genetic findings were also crucial in deciding the treatment modalities in a few cases. Cases harbouring defects leading to antibody deficiencies were placed on regular replacement intravenous immunoglobulin therapy.

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Utility of targeted next generation sequencing for inborn errors of immunity at a tertiary care centre in North India | Scientific Reports -...