Category Archives: Transhuman News

Scientists study structure of DNA from mammoths preserved in Arctic
A huge step towards recreating the woolly mammoth has been taken by scientists who inserted more than a dozen of its genes into the live DNA of an elephant. Researchers studied the structure...

By: Nature Travel

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Scientists study structure of DNA from mammoths preserved in Arctic - Video

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AW: "DOUBLE DNA BOMB wARX" - Video

Stardoll Makeup Tutorial - Jade Thirlwall DNA Album Inspired Look.
A Stardoll makeup look inspired by Jade Thirlwall #39;s look on Little Mix #39;s debut album DNA.

By: iBHADDCHICA SD

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Stardoll Makeup Tutorial - Jade Thirlwall DNA Album Inspired Look. - Video

DNA: The disturbing case of shrinking farmer population in India
Recent reports and figures reveal a shrinking farmer population in India, which is primarily an agriculture-based country.

By: Zee News

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DNA: The disturbing case of shrinking farmer population in India - Video

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By: Fil Barao

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RANDOM DNA BOMB contra bots - Video

Cod Advanced Warfare DNA bomb FAIL+RAGE Ep.1
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By: RobBobs125

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Cod Advanced Warfare DNA bomb FAIL+RAGE Ep.1 - Video

DNA Ispoetka
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By: Zanil Tataj

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DNA Ispoetka - Video

A technique for editing genes while they reside in intact chromosomes has been a real breakthrough. Literally. In 2013, Science magazine named it the runner-up for breakthrough-of-the-year, andits developers won the 2015 Breakthrough Prize.

The system being honored is called CRISPR/Cas9, and it evolved as a way for bacteria to destroy viruses using RNA that matched the virus' DNA sequence. But it's turned out to be remarkably flexible, and the technique can be retargeted to any gene simply by modifying the RNA. Researchers are still figuring out new uses for the system, which means there are papers coming out nearly every week, many of them difficult to distinguish.

Thatmay bepreciselywhy the significance of a paper published last week wasn't immediately obvious. In it, the authors described a way of ensuring that if one copy of a gene was modified by CRISPR/Cas9, the second copy would beuseful, but not revolutionary. What may have been missed was that this process doesn't stop once those two copies are modified. Instead, it happens in the next generation as well, and then the generation after that. In fact, the modified genes could spread throughout an entire species in a chain reaction, a fact that has raised ethical and safety concerns about the work.

The CRISPR/Cas9 system is remarkably simple. It relies on RNA molecules that have a specific format and are able to base pair with a site in the genome. Cas9 then cuts the DNA at the site where this base pairing occurs, creating a break in the chromosome. Cells have systems that attempt to repair these breaks, and these systems attempt to identify similar-looking sequences to use as a template for repair. So if you provide the cells with some similar DNA, it will end up being placed at the site that the RNA first targeted.

This makes it easy to modify the genome. By providing slightly different DNA to be used in the repair process, you can substitute altered bases, short deletions, or even entire additional genes, any of which can take their place within the chromosome. In short, CRISPR/Cas9 lets you put any DNA you want anywhere in a genome.

It's possible to use this to eliminate genes you're interested in, so you can study animals that lack that gene. You simply target the gene with an RNA, and then provide DNA with a deletion of a key part of the gene. The repair system will use the deletion as part of its template and copy it into place on the chromosome. It's also possible to mutate a gene by replacing key parts of it with something else. For example, you could swap in a copy of the Green Fluorescent protein and ensure that all of the resulting mutants glow green.

But you still have to breed these mutations the old-fashioned way: you need to get two organisms that have a copy of the mutant gene, then breed them together. Mendel then tells us that one-quarter of the offspring will have mutant copies in both of their chromosomes.

The authors of the new paper found that frustratingly slow. Working in flies, they designed a system where CRISPR/Cas9 would do all the work for them. Their DNA repair template was a bit more complicated than a simple deletion. Instead, it contained the genes needed to get the CRISPR/Cas9 system to work, along with a guide RNA that targeted a specific fly gene (in this case, yellow). They surrounded all these genes with DNA from the yellow gene itself.

Once injected in the fly, the normal yellow gene was disrupted by the genes for the CRISPR/Cas9 system. Once that happened on one chromosome, the system could easily perform the same modification on the other chromosome, making the animal a homozygous yellow mutant.

But the key thing is what happens in the next generation. In these animals, a normal copy of the yellow gene comes in from the next parent. But the CRISPR/Cas9 cassette immediately converts that, too, resulting in offspring that are all yellow. Well, not all; but the authors found that the construct was 97 percent effective at converting the next generation. In fact, there's nothing to stop this system from invading an entire population, continuing to convert generation after generation until everything carries the modification.

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New DNA construct can set off a mutagenic chain reaction

DEKALB, TX (KSLA) -

DNA testing has been arranged to confirm the quadruplet calves born to a DeKalb, TX cow last week.

Owner Jimmy Barling says all 4 calves, now one week old, are getting around and doing well.

In fact, they say Baby Moo is "running and jumping and curious," and that "he and his mother appear to be getting along every well together, which is a good sign."

Moo is one of the 3 males born to the cow, identified as #15.

Siblings Eeny, Meeny and the heifer, Miney are with surrogate families and friends of the Barlings.

The odds of a cow having quadruplets with all 4 surviving are said to be more than one in 11 million.

"We weren't expecting for it to happen," Barling says of the quadruple calving, "and then just, 'Bang!' It happened right in front of our eyes."

In spite of witnessing the births, Barling says he and his wife Dora have decided to go ahead with DNA testing.

"So that way, we've got scientific validation that these are, even if something happens to one of them, we've got proof that they're all siblings, and the mother really is the mother," Barling explains.

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DNA testing ordered on rare set of DeKalb, TX quadruplet calves