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How to Choke a DNA Bomb THE HARD WAY. (Advanced Warfare)
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How to Choke a DNA Bomb THE HARD WAY. (Advanced Warfare) - Video

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#4 DOUBLE DNA BOMB on Advance Warfare
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By: jheson suarez

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#4 DOUBLE DNA BOMB on Advance Warfare - Video

A clearer understanding of the origin recognition complex (ORC) -- a protein complex that directs DNA replication -- through its crystal structure offers new insight into fundamental mechanisms of DNA replication initiation. This will also provide insight into how ORC may be compromised in a subset of patients with Meier-Gorlin syndrome, a form of dwarfism in humans.

ORC is a six-subunit protein complex that directly binds DNA to recruit other protein factors involved in DNA replication. Researchers collected data at the Advanced Photon Source (APS), a U.S. Department of Energy User Facility based at Argonne National Laboratory, to obtain the first atomic-level resolution picture of this complex.

The structure shows that ORC's main body has five subunits that contain a common fold that is found in proteins binding ATP, a small molecule that cells use as fuel. One of the largest subunits, ORC3, has a structural element that protrudes from the ORC core to contact ORC6, according to the paper, "Crystal Structure of the Eukaryotic Origin Recognition Complex," published last week in Nature.

"The crystal structure explains why a mutation in ORC6 that is linked to Meier-Gorlin syndrome in a subset of patients results in defective binding of this subunit to ORC3," said Franziska Bleichert, the paper's lead author. "The structure also makes specific predictions on how the different ORC protein subunits might interact with DNA in the central channel of ORC and with other replication initiation factors."

The research team was surprised to learn that a region of subunit ORC1, which is biochemically known to interact with a similar region of ORC4, did not do so in its structure. In fact, this region in ORC1 blocks entry into the central channel and prevents DNA binding. This phenomenon suggests that ORC in multi-cellular organisms can adopt a previously unanticipated inactivated state that is likely important to regulating its activity and preventing inappropriate DNA replication.

Researchers' access to the APS was important. "The powerful X-ray beam at the APS and the flexible data collection technologies at the (General Medical Sciences and National Cancer Institute (GM/CA) beamlines) -- in particular microfocus capabilities and crystal rastering -- were critical in obtaining high-quality datasets that allowed us to determine the structure," Bleichert said.

The research illustrates the importance of understanding the role proteins have in DNA replication, an essential cellular process that needs to be tightly regulated. When deviations in DNA replication occur, cells can end up with fragmented DNA that can lead to cell death and diseases such as cancer.

The paper was published online in Nature.

The paper authors are Bleichert and James Berger, Johns Hopkins University School of Medicine, and Michael Botchan, the University of California (UC), Berkeley.

APS beamline 23-ID-B was used for research.

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Improved understanding of protein complex offers insight into DNA replication initiation mechanism basics

OLYMPIA, Wash. (AP) - The Senate on Wednesday passed a bill that would require DNA collected in any felony case charged as a violent or sex offense to be preserved through the length of the offenders sentence, including post-prison community custody.

House Bill 1069 passed the chamber on a 47-1 vote, but it heads back to the House - where it first passed in February - because of changes made in a Senate committee.

The reasons we need this bill is that DNA evidence has the power to vindicate the innocent and convict the guilty, said Republican Sen. Mike Padden of Spokane, who is chairman of the Senate Law and Justice Committee that heard the bill.

Most states have varying laws on how long to preserve biological evidence. Washington is one of several that dont automatically preserve DNA for serious felonies. Under current state law, once convicted, defendants must file motions to have evidence preserved for use during possible appeals.

Under the original bill, in cold cases, where no one has been charged or convicted, the DNA would have to be maintained throughout the statute of limitations for the crime. Law enforcement groups expressed concerns that because some crimes, like murder, have no statute of limitations, they could be on the hook to hold the evidence forever. The changes made in the Senate changes that timeframe for holding the DNA at either 99 years or the statute of limitations, whichever is shorter.

The lone no vote was from Democratic Sen. Bob Hasegawa of Seattle, who said he had concerns about the language in the bill that keeps the DNA material after someone is found not guilty, or where charges are dismissed with prejudice, unless that person applies to the Washington State Patrol to have the material expunged.

The bill clarifies that nothing keeps a trial could from ordering destruction of the samples from a defendant who was charged and acquitted, or whose conviction was overturned.

Rep. Tina Orwall, a Democrat from Des Moines who sponsored the bill, said she was happy with the changes made in the Senate and will recommend that the House concur with the changes.

I believe this is a reasonable approach for this ground-breaking legislation, she wrote in an email.

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Senate OKs post-conviction preservation of DNA