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Category Archives: Transhuman News

JET 14(1) – April 2005 – Bostrom – Transhumanist Thought

Posted: June 19, 2016 at 3:28 am

Nick Bostrom Faculty of Philosophy, Oxford University

Journal of Evolution and Technology - Vol. 14 Issue 1 - April 2005 http://jetpress.org/volume14/bostrom.html

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This paper traces the cultural and philosophical roots of transhumanist thought and describes some of the influences and contributions that led to the development of contemporary transhumanism.

The human desire to acquire new capacities is as ancient as our species itself. We have always sought to expand the boundaries of our existence, be it socially, geographically, or mentally. There is a tendency in at least some individuals always to search for a way around every obstacle and limitation to human life and happiness.

Ceremonial burial and preserved fragments of religious writings show that prehistoric man and woman were deeply disturbed by the death of loved ones. Although the belief in an afterlife was common, this did not preclude efforts to extend the present life. In the Sumerian Epic of Gilgamesh (approx. 1700 B.C.), a king sets out on a quest for immortality. Gilgamesh learns that there exists a natural means an herb that grows at the bottom of the sea.[1] He successfully retrieves the plant, but a snake steals it from him before he can eat it. In later times, explorers sought the Fountain of Youth, alchemists labored to concoct the Elixir of Life, and various schools of esoteric Taoism in China strove for physical immortality by way of control over or harmony with the forces of nature. The boundary between mythos and science, between magic and technology, was blurry, and almost all conceivable means to the preservation of life were attempted by somebody or other. Yet while explorers made many interesting discoveries and alchemists invented some useful things, such as new dyes and improvements in metallurgy, the goal of life-extension proved elusive.

The quest to transcend our natural confines, however, has long been viewed with ambivalence. On the one hand there is fascination. On the other there is the concept of hubris: that some ambitions are off-limits and will backfire if pursued. The ancient Greeks exhibited this ambivalence in their mythology. Prometheus stole the fire from Zeus and gave it to the humans, thereby permanently improving the human condition. Yet for this act he was severely punished by Zeus. In the myth of Daedalus, the gods are repeatedly challenged, quite successfully, by the clever engineer and artist who uses non-magical means to extend human capabilities. In the end, however, disaster ensues when his son Icarus ignores paternal warnings and flies too close to the sun, causing the wax in his wings to melt.

Medieval Christianity had similarly conflicted views about the pursuits of the alchemists, who tried to transmute substances, create homunculi in test tubes, and invent a panacea. Some scholastics, following the anti-experimentalist teachings of Aquinas, believed that alchemy was an ungodly activity. There were allegations that it involved the invocation of daemonic powers. But other theologians, such as Albertus Magnus, defended the practice.[2]

The otherworldliness and stale scholastic philosophy that dominated Europe during the Middle Ages gave way to a renewed intellectual vigor in the Renaissance. The human being and the natural world again became legitimate objects of study. Renaissance humanism encouraged people to rely on their own observations and their own judgment rather than to defer in every matter to religious authorities. Renaissance humanism also created the ideal of the well-rounded person, one who is highly developed scientifically, morally, culturally, and spiritually. A landmark of the period is Giovanni Pico della Mirandolas Oration on the Dignity of Man (1486), which proclaims that man does not have a readymade form and is responsible for shaping himself:

We have made you a creature neither of heaven nor of earth, neither mortal nor immortal, in order that you may, as the free and proud shaper of your own being, fashion yourself in the form you may prefer. It will be in your power to descend to the lower, brutish forms of life; you will be able, through your own decision, to rise again to the superior orders whose life is divine.[3]

The Age of Enlightenment is often said to have started with the publication of Francis Bacons Novum Organum, the new tool " (1620), which proposes a scientific methodology based on empirical investigation rather than a priori reasoning.[4]Bacon advocated the project of "effecting all things possible, " by which he meant using science to achieve mastery over nature in order to improve the living condition of human beings. The heritage from the Renaissance combines with the influence of Isaac Newton, Thomas Hobbes, John Locke, Immanuel Kant, the Marquis de Condorcet, and others to form the basis for rational humanism, which emphasizes empirical science and critical reason rather than revelation and religious authority as ways of learning about the natural world and our place within it, and of providing a grounding for morality. Transhumanism has roots in rational humanism.

In the 18th and 19th centuries we begin to see glimpses of the idea that even humans themselves can be developed through the appliance of science. Condorcet speculated about extending human life span through medical science:

Would it be absurd now to suppose that the improvement of the human race should be regarded as capable of unlimited progress? That a time will come when death would result only from extraordinary accidents or the more and more gradual wearing out of vitality, and that, finally, the duration of the average interval between birth and wearing out has itself no specific limit whatsoever? No doubt man will not become immortal, but cannot the span constantly increase between the moment he begins to live and the time when naturally, without illness or accident, he finds life a burden?"[5]

Benjamin Franklin longed wistfully for suspended animation, foreshadowing the cryonics movement:

I wish it were possible... to invent a method of embalming drowned persons, in such a manner that they might be recalled to life at any period, however distant; for having a very ardent desire to see and observe the state of America a hundred years hence, I should prefer to an ordinary death, being immersed with a few friends in a cask of Madeira, until that time, then to be recalled to life by the solar warmth of my dear country! But... in all probability, we live in a century too little advanced, and too near the infancy of science, to see such an art brought in our time to its perfection.[6]

After the publication of Darwins Origin of Species (1859), it became increasingly plausible to view the current version of humanity not as the endpoint of evolution but rather as a possibly quite early phase."[7]The rise of scientific physicalism might also have contributed to the foundations of the idea that technology could be used to improve the human organism. For example, a simple kind of materialist view was boldly proposed in 1750 by the French physician and materialist philosopher, Julien Offray de La Mettrie in LHomme Machine, where he argued that "man is but an animal, or a collection of springs which wind each other up. "[8]If human beings are constituted by matter that obeys the same laws of physics that operate outside us, then it should in principle be possible to learn to manipulate human nature in the same way that we manipulate external objects.

It has been said that the Enlightenment expired as the victim of its own excesses. It gave way to Romanticism, and to latter day reactions against the rule of instrumental reason and the attempt to rationally control nature, such as can be found in some postmodernist writings, the New Age movement, deep environmentalism, and in some parts of the anti-globalization movement. However, the Enlightenments legacy, including a belief in the power of human rationality and science, is still an important shaper of modern culture. In his famous 1784 essay "What Is Enlightenment? ", Kant summed it up as follows:

Enlightenment is mans leaving his self-caused immaturity. Immaturity is the incapacity to use ones own understanding without the guidance of another. Such immaturity is self-caused if its cause is not lack of intelligence, but by lack of determination and courage to use ones intelligence without being guided by another. The motto of enlightenment is therefore: Sapere aude! Have courage to use your own intelligence!"[9]

It might be thought that the German philosopher Friedrich Nietzsche (1844-1900) would have been a major inspiration for transhumanism. Nietzsche is famous for his doctrine of der bermensch (the overman "):

I teach you the overman. Man is something that shall be overcome. What have you done to overcome him? All beings so far have created something beyond themselves; and do you want to be the ebb of this great flood and even go back to the beasts rather than overcome man?"[10]

What Nietzsche had in mind, however, was not technological transformation but rather a kind of soaring personal growth and cultural refinement in exceptional individuals (who he thought would have to overcome the life-sapping "slave-morality " of Christianity). Despite some surface-level similarities with the Nietzschean vision, transhumanism with its Enlightenment roots, its emphasis on individual liberties, and its humanistic concern for the welfare of all humans (and other sentient beings) probably has as much or more in common with Nietzsches contemporary J.S. Mill, the English liberal thinker and utilitarian.

In 1923, the noted British biochemist J. B. S. Haldane published the essay Daedalus: Science and the Future, in which he argued that great benefits would come from controlling our own genetics and from science in general. He projected a future society that would be richer, have abundant clean energy, where genetics would be employed to make people taller, healthier, and smarter, and where the use of ectogenesis (gestating fetuses in artificial wombs) would be commonplace. He also commented on what has in more recent years become known as the "yuck factor ":

The chemical or physical inventor is always a Prometheus. There is no great invention, from fire to flying, which has not been hailed as an insult to some god. But if every physical and chemical invention is a blasphemy, every biological invention is a perversion. There is hardly one which, on first being brought to the notice of an observer from any nation which has not previously heard of their existence, would not appear to him as indecent and unnatural.[11]

Haldanes essay became a bestseller and set off a chain reaction of future-oriented discussions, including The World, the Flesh and the Devil, by J. D. Bernal (1929)[12], which speculated about space colonization and bionic implants as well as mental improvements through advanced social science and psychology; the works of Olaf Stapledon, a philosopher and science fiction author; and the essay "Icarus: the Future of Science " (1924) by Bertrand Russell.[13]Russell took a more pessimistic view, arguing that without more kindliness in the world, technological power would mainly serve to increase mens ability to inflict harm on one another. Science fiction authors such as H. G. Wells and Stapledon got many people thinking about the future evolution of the human race.

Aldous Huxleys Brave New World, published in 1932, has had an enduring impact on debates about human technological transformation[14]matched by few other works of fiction (a possible exception would be Mary Shelleys Frankenstein, 1818[15]). Huxley describes a dystopia where psychological conditioning, promiscuous sexuality, biotechnology, and the opiate drug "soma " are used to keep the population placid and contented in a static, totally conformist caste society that is governed by ten world controllers. Children are manufactured in fertility clinics and artificially gestated. The lower castes are chemically stunted or deprived of oxygen during their maturation process to limit their physical and intellectual development. From birth, members of every caste are indoctrinated during their sleep, by recorded voices repeating the slogans of the official "Fordist " religion, and are conditioned to believe that their own caste is the best one to belong to. The society depicted in Brave New World is often compared and contrasted with that of another influential 20th century dystopia, George Orwells 1984.[16] 1984 features a more overt form of oppression, including ubiquitous surveillance by "Big Brother " and brutal police coercion. Huxleys world controllers, by contrast, rely on more "humane means ", including bio-engineered predestination, soma, and psychological conditioning to prevent people from wanting to think for themselves. Herd-mentality and promiscuity are promoted, while high art, individuality, knowledge of history, and romantic love are discouraged. It should be noted that in neither 1984 nor Brave New World has technology been used to increase human capacities. Rather, society is set up to repress the full development of humanity. Both dystopias curtail scientific and technological exploration for fear of upsetting the social equilibrium. Nevertheless, Brave New World in particular has become an emblem of the dehumanizing potential of the use of technology to promote social conformism and shallow contentment.

In the postwar era, many optimistic futurists who had become suspicious of collectively orchestrated social change found a new home for their hopes in scientific and technological progress. Space travel, medicine, and computers seemed to offer a path to a better world. The shift of attention also reflected the breathtaking pace of development taking place in these fields. Science had begun to catch up with speculation. Yesterdays science fiction was turning into todays science fact or at least into a somewhat realistic mid-term prospect.

Transhumanist themes during this period were discussed and analyzed chiefly in the science fiction literature. Authors such as Arthur C. Clarke, Isaac Asimov, Robert Heinlein, and Stanislaw Lem explored how technological development could come to profoundly alter the human condition.

The word "transhumanism " appears to have been first used by Aldous Huxleys brother, Julian Huxley, a distinguished biologist (who was also the first director-general of UNESCO and founder of the World Wildlife Fund). In Religion Without Revelation (1927), he wrote:

The human species can, if it wishes, transcend itself not just sporadically, an individual here in one way, an individual there in another way but in its entirety, as humanity. We need a name for this new belief. Perhaps transhumanism will serve: man remaining man, but transcending himself, by realizing new possibilities of and for his human nature.[17]

Human-like automata have always fascinated the human imagination. Mechanical engineers since the early Greeks have constructed clever self-moving devices.

In Judaic mysticism, a "golem " refers to an animated being crafted from inanimate material. In the early golem stories, a golem could be created by a holy person who was able to share some of Gods wisdom and power (although the golem, not being able to speak, was never more than a shadow of Gods creations). Having a golem servant was the ultimate symbol of wisdom and holiness. In the later stories, which had been influenced by the more Islamic concern about humanity getting too close to God, the golem became a creation of overreaching mystics, who would inevitably be punished for their blasphemy. The story of the Sorcerers Apprentice is a variation of this theme: the apprentice animates a broomstick to fetch water but is unable to make the broom stop like Frankenstein, a story of technology out of control. The word "robot " was coined by the Czech Karel apeks in his dark play R.U.R. (1921), in which a robot labor force destroys its human creators.[18]With the invention of the electronic computer, the idea of human-like automata graduated from the kindergarten of mythology to the school of science fiction (e.g. Isaac Asimov, Stanislav Lem, Arthur C. Clark) and eventually to the college of technological prediction.

Could continued progress in artificial intelligence lead to the creation of machines that can think in the same general way as human beings? Alan Turing gave an operational definition to this question in his classic "Computing Machinery and Intelligence " (1950), and predicted that computers would eventually pass what came to be known as the Turing Test. (In the Turing Test, a human experimenter interviews a computer and another human via a text interface, and the computer succeeds if the interviewer cannot reliably distinguish the computer from the human.)[19]Much ink has been spilt in debates on whether this test furnishes a necessary and sufficient condition for a computer being able to think, but what matters more from a practical perspective is whether and, and if so when, computers will be able to match human performance on tasks involving general reasoning ability. With the benefit of hindsight, we can say that many of the early AI researchers turned out to be overoptimistic about the timescale for this hypothetical development. Of course, the fact that we have not yet reached human-level artificial intelligence does not mean that we never will, and a number of people, e.g. Marvin Minsky, Hans Moravec, Ray Kurzweil, and Nick Bostrom have put forward reasons for thinking that this could happen within the first half of this century.[20]

In 1958, Stanislaw Ulam, referring to a meeting with John von Neumann, wrote:

One conversation centered on the ever accelerating progress of technology and changes in the mode of human life, which gives the appearance of approaching some essential singularity in the history of the race beyond which human affairs, as we know them, could not continue.[21]

The rapidity of technological change in recent times leads naturally to the idea that continued technological innovation will have a large impact on humanity in the decades ahead. This prediction is strengthened if one believes that some of those variables that currently exhibit exponential growth will continue to do so and that they will be among the main drivers of change. Gordon E. Moore, co-founder of Intel, noticed in 1965 that the number of transistors on a chip exhibited exponential growth. This led to the formulation of "Moores law ", which states (roughly) that computing power doubles every 18 months to two years.[22]More recently, Kurzweil has documented similar exponential growth rates in a number of other technologies. (The world economy, which is a kind of general index of humanitys productive capacity, has doubled about every 15 years in modern times.)

The singularity hypothesis, which von Neumann seems to have alluded to in the quoted passage above, is that these changes will lead to some kind of discontinuity. But nowadays, it often refers to a more specific prediction, namely that the creation of self-improving artificial intelligence will at some point result in radical changes within a very short time span. This hypothesis was first clearly stated in 1965 by the statistician I. J. Good:

Let an ultraintelligent machine be defined as a machine that can far surpass all the intellectual activities of any man however clever. Since the design of machines is one of these intellectual activities, an ultraintelligent machine could design even better machines; there would then unquestionably be an intelligence explosion, and the intelligence of man would be left far behind. Thus the first ultraintelligent machine is the last invention that man need ever make.[23]

Vernor Vinge discussed this idea in a little more detail in his influential 1993-paper "Technological Singularity ", in which he predicted:

Within thirty years, we will have the technological means to create superhuman intelligence. Shortly after, the human era will be ended.[24]

Transhumanists today hold diverging views about the singularity: some see it as a likely scenario, others believe that it is more probable that there will never be any very sudden and dramatic changes as the result of progress in artificial intelligence.

The singularity idea also comes in a somewhat different eschatological version, which traces its lineage to the writings of Pierre Teilhard de Chardin, a paleontologist and Jesuit theologian who saw an evolutionary telos in the development of an encompassing noosphere (a global consciousness) via physicist Frank Tipler, who argued that advanced civilizations might come to have a defining influence on the future evolution of the cosmos, and, in the final moments of the Big Crunch, might manage to extract an infinite number of computations by harnessing the sheer energy of the collapsing matter.[25],[26] However, while these ideas might appeal to those who fancy a marriage between mysticism and science, they have not caught on either among transhumanists or the larger scientific community. Current cosmological theories indicate that the universe will continue to expand forever (falsifying Tiplers prediction). But the more general point that the transhumanist might make in this context is that we need to learn to think about "big-picture questions " without resorting to wishful thinking or mysticism. Big-picture questions, including ones about our place in the world and the long-term fate of intelligent life are part of transhumanism; however, these questions should be addressed in a sober, disinterested way, using critical reason and our best available scientific evidence. One reason why such questions are of transhumanist interest is that their answers might affect what outcomes we should expect from our own technological development, and therefore indirectly what policies it makes sense for humanity to pursue.

In 1986, Eric Drexler published Engines of Creation, the first book-length exposition of molecular manufacturing.[27](The possibility of nanotechnology had been anticipated by Nobel laureate physicist Richard Feynman in his famous after-dinner address in 1959 entitled There is Plenty of Room at the Bottom ".[28]) In this seminal work, Drexler not only argued for the feasibility of assembler-based nanotechnology but also explored its consequences and began charting the strategic challenges posed by its development. Drexlers later book Nanosystems (1992) supplied a more technical analysis that seemed to confirm his original conclusions.[29] To prepare the world for nanotechnology and work towards its safe implementation, he founded the Foresight Institute together with his then wife, Christine Peterson, in 1986.

In the last several years, nanotechnology has become big business, with worldwide research funding amounting to billions of dollars. Yet little of this work fits Drexlers ambitious vision of nanotechnology as an assembler-based, near-universal, construction technology. The mainstream nanotechnology community has sought to distance itself from Drexlers claims. The chemist Richard Smalley (another Noble laureate) has debated Drexler, asserting that non-biological molecular assemblers are impossible.[30]To date, however, no technical critique of Drexlers work in the published literature has found any significant flaws in his reasoning. If molecular nanotechnology is indeed physically possible, as Drexler maintains, the question becomes just how difficult it will be to develop it, and how long it will take. These issues are very difficult to settle in advance.

If molecular nanotechnology could be developed as Drexler envisions it, it would have momentous ramifications:

Coal and diamonds, sand and computer chips, cancer and healthy tissue: throughout history, variations in the arrangement of atoms have distinguished the cheap from the cherished, the diseased from the healthy. Arranged one way, atoms make up soil, air, and water arranged another, they make up ripe strawberries. Arranged one way, they make up homes and fresh air; arranged another, they make up ash and smoke.[31]

Molecular nanotechnology would enable us to transform coal into diamonds, sand into supercomputers, and to remove pollution from the air and tumors from healthy tissue. In its mature form, it could help us abolish most disease and aging, make possible the reanimation of cryonics patients, enable affordable space colonization, and more ominously lead to the rapid creation of vast arsenals of lethal or non-lethal weapons.

Another hypothetical technology that would have a revolutionary impact is uploading, the transfer of a human mind to a computer. This would involve the following steps: First, create a sufficiently detailed scan of a particular human brain, perhaps by deconstructing it with nanobots or by feeding thin slices of brain tissues into powerful microscopes for automatic image analysis. Second, from this scan, reconstruct the neuronal network that the brain implemented, and combine this with computational models of the different types of neurons. Third, emulate the whole computational structure on a powerful supercomputer. If successful, the procedure would result in the original mind, with memory and personality intact, being transferred to the computer where it would then exist as software; and it could either inhabit a robot body or live in a virtual reality.[32]While it is often thought that, under suitable circumstances, the upload would be conscious and that the original person would have survived the transfer to the new medium, individual transhumanists take different views on these philosophical matters.

If either superintelligence, or molecular nanotechnology, or uploading, or some other technology of a similarly revolutionary kind is developed, the human condition could clearly be radically transformed. Even if one believed that the probability of this happening any time soon is quite small, these prospects would nevertheless merit serious attention in view of their extreme impact. However, transhumanism does not depend on the feasibility of such radical technologies. Virtual reality; preimplantation genetic diagnosis; genetic engineering; pharmaceuticals that improve memory, concentration, wakefulness, and mood; performance-enhancing drugs; cosmetic surgery; sex change operations; prosthetics; anti-aging medicine; closer human-computer interfaces: these technologies are already here or can be expected within the next few decades. The combination of these technological capabilities, as they mature, could profoundly transform the human condition. The transhumanist agenda, which is to make such enhancement options safely available to all persons, will become increasingly relevant and practical in the coming years as these and other anticipated technologies come online.

Benjamin Franklin wished to be preserved in a cask of Madeira and later recalled to life, and regretted that he was living too near the infancy of science for this to be possible. Since then, science has grown up a bit. In 1962, Robert Ettinger published the book, The Prospect of Immortality, which launched the idea of cryonic suspension.[33]Ettinger argued that as medical technology seems to be constantly progressing, and since science has discovered that chemical activity comes to a complete halt at low-enough temperatures, it should be possible to freeze a person today (in liquid nitrogen) and preserve the body until a time when technology is advanced enough to repair the freezing damage and reverse the original cause of deanimation. Cryonics, Ettinger believed, offered a ticket to the future.

Alas, the masses did not line up for the ride. Cryonics has remained a fringe alternative to more traditional methods of treating the terminally diseased, such as cremation and burial. The practice of cryonics was not integrated into the mainstream clinical setting and was instead conducted on the cheap by a small number of enthusiasts. Two early cryonics organizations went bankrupt, allowing their patients to thaw out. At that point, the problem of massive cellular damage that occurs when ice crystals form in the body also became more widely known. As a result, cryonics acquired a reputation as a macabre scam. The media controversy over the suspension of baseball star Ted Williams in 2002 showed that public perception of cryonics has not changed much over the past decades.

Despite its image problem and its early failures of implementation, the cryonics community continues to be active and it counts among its members several eminent scientists and intellectuals. Suspension protocols have been improved, and the infusion of cryoprotectants prior to freezing to suppress the formation of ice crystals has become standard practice. The prospect of nanotechnology has given a more concrete shape to the hypothesized future technology that could enable reanimation. There are currently two organizations that offer full-service suspension, the Alcor Life Extension Foundation (founded in 1972) and the Cryonics Institute (founded in 1976). Alcor has recently introduced a new suspension method, which relies on a process known as "vitrification ", which further reduces micro-structural damage during suspension.

In a later work, Man into Superman (1972), Ettinger discussed a number of conceivable technological improvements of the human organism, continuing the tradition started by Haldane and Bernal.[34]

Another early transhumanist was F. M. Esfandiary, who later changed his name to FM-2030. One of the first professors of future studies, FM taught at the New School for Social Research in New York in the 1960s and formed a group of optimistic futurists known as the UpWingers.

Who are the new revolutionaries of our time? They are the geneticists, biologists, physicists, cryonologists, biotechnologists, nuclear scientists, cosmologists, radio astronomers, cosmonauts, social scientists, youth corps volunteers, internationalists, humanists, science-fiction writers, normative thinkers, inventors They and others are revolutionizing the human condition in a fundamental way. Their achievements and goals go far beyond the most radical ideologies of the Old Order.[35]

In his book Are you a transhuman? (1989), FM described what he regarded as the signs of the emergence of the "transhuman ".[36]In FMs terminology, a transhuman is a "transitional human, " someone who by virtue of their technology usage, cultural values, and lifestyle constitutes an evolutionary link to the coming era of posthumanity. The signs that FM saw as indicative of transhuman status included prostheses, plastic surgery, intensive use of telecommunications, a cosmopolitan outlook and a globetrotting lifestyle, androgyny, mediated reproduction (such as in vitro fertilization), absence of religious belief, and a rejection of traditional family values. However, it was never satisfactorily explained why somebody who, say, rejects family values, has a nose job, and spends a lot of time on jet planes is in closer proximity to posthumanity than the rest of us.

In the 1970s and 1980s, many organizations sprang up that focused on a particular topic such as life extension, cryonics, space colonization, science fiction, and futurism. These groups were often isolated from one another, and whatever shared views and values they had did not yet amount to any unified worldview. Ed Regiss Great Mambo Chicken and the Transhuman Condition (1990) took a humorous look at these proto-transhumanist fringes, which included eccentric and otherwise intelligent individuals trying to build space rockets in their backyards or experimenting with biofeedback machines and psychedelic drugs, as well as scientists pursuing more serious lines of work but who had imbibed too deeply of the Californian spirit.[37]

In 1988, the first issue of the Extropy Magazine was published by Max More and Tom Morrow, and in 1992 they founded the Extropy Institute (the term "extropy " being coined as a metaphorical opposite of entropy). The Institute served as a catalyst that brought together disparate groups of people with futuristic ideas and facilitated the formation of novel memetic compounds. The Institute ran a series of conferences, but perhaps most important was the extropians mailing list, an online discussion forum where new ideas were shared and debated. In the mid-nineties, many got first exposure to transhumanist views from the Extropy Institutes listserve.

More had immigrated to California from Britain after changing his name from Max OConnor. Of his new name, he said:

It seemed to really encapsulate the essence of what my goal is: always to improve, never to be static. I was going to get better at everything, become smarter, fitter, and healthier. It would be a constant reminder to keep moving forward.[38]

Max More wrote the first definition of transhumanism in its modern sense, and created his own distinctive brand of transhumanism, "extropianism, " which emphasized the principles of "boundless expansion, " "self-transformation, " "dynamic optimism, " "intelligent technology, " and "spontaneous order ". Originally, extropianism had a clear libertarian flavor, but in later years More has distanced himself from this ingredient, replacing "spontaneous order " with open society, " a principle that opposes authoritarian social control and promotes decentralization of power and responsibility.[39]

Natasha Vita-More (married to Max) is the Extropy Institutes current president. She is an artist and designer, and has over the years issued a number of manifestos on transhumanist and extropic art.[40]

The Extropy Institutes conferences and mailing list also served as a hangout place for some people who liked to discuss futuristic ideas but who were not necessarily joiners. Those who were around in the mid-nineties will remember individuals such as Anders Sandberg, Alexander "Sasha " Chislenko, Hal Finney, and Robin Hanson from among the more thoughtful regulars in the transhumanist milieu at the time. An enormous amount of discussion about transhumanism has taken place on various email lists in the past decade. The quality of postings has been varied (putting it mildly). Yet at their best, these online conversations explored ideas about the implications of future technologies that were, in some respects, far advanced over what could be found in printed books or journals. The Internet played an important role in incubating modern transhumanism by facilitating these meetings of minds and perhaps more indirectly, too, via the "irrational exuberance " that pervaded the dot-com era?

The World Transhumanist Association was founded in early 1998 by Nick Bostrom and David Pearce, to provide a general organizational basis for all transhumanist groups and interests, across the political spectrum. The aim was also to develop a more mature and academically respectable form of transhumanism, freed from the "cultishness " which, at least in the eyes of some critics, had afflicted some of its earlier convocations. The two founding documents of the WTA were the Transhumanist Declaration (see appendix), and the Transhumanist FAQ (v. 1.0).[41]The Declaration was intended as a concise consensus statement of the basic principle of transhumanism. The FAQ was also a consensus or near-consensus document, but it was more ambitious in its philosophical scope in that it developed a number of themes that had previously been, at most, implicit in the movement. More than fifty people contributed comments on drafts of the FAQ. The document was produced by Bostrom but major parts and ideas were also contributed by several others, including the British utilitarian thinker David Pearce, Max More, the American feminist and disability rights activist Kathryn Aegis, and the walking encyclopedia Anders Sandberg, who was at the time a neuroscience student in Sweden.

A number of related organizations have also cropped up in recent years, focusing more narrowly on particular transhumanist issues, such as life-extension, artificial intelligence, or the legal implications of "converging technologies " (nano-bio-info-neuro technologies). The Institute for Ethics and Emerging Technologies, a non-profit think tank, was established in 2004, to "promote the ethical use of technology to expand human capacities ".

Over the past couple of decades, academia has picked up the ball and started to analyze various "transhumanist matters, " both normative and positive. The contributions are far too many to comprehensively describe here, so we will pick out just a few threads, beginning with ethics.

For most of its history, moral philosophy did not shy away from addressing practical problems. In the early and mid-parts of the twentieth century, during heydays of logical positivism, applied ethics became a backwater as moral philosophers concentrated on linguistic or meta-ethical problems. Since then, however, practical ethics has reemerged as a field of academic inquiry. The comeback started in medical ethics. Revelations of the horrific experiments that the Nazis had conducted on human subjects in the name of science led to the adoption of the Nuremberg code (1947) and the Declaration of Helsinki (1964), which laid down strict safeguards for medical experimentation, emphasizing the need for patient consent.[42],[43] But the rise of the modern health care system spawned new ethical dilemmas turning off life-support, organ donation, resource allocation, abortion, advance directives, doctor-patient relationships, protocols for obtaining informed consent and for dealing with incompetent patients. In the 1970s, a broader kind of enquiry began to emerge, stimulated particularly by developments in assisted reproduction and genetics. This field became known as bioethics. Many of the ethical issues most directly linked to transhumanism would now fall under this rubric, although other normative discourses are also involved, e.g. population ethics, meta-ethics, political philosophy, and bioethics younger sisters computer ethics, engineering ethics, environmental ethics.

Bioethics was from the beginning an interdisciplinary endeavor, dominated by theologians, legal scholars, physicians, and, increasingly, philosophers, with occasional participation by representatives of patients rights groups, disability advocates, and other interested parties. [44] Lacking a clear methodology, and operating on a plain often swept by the winds of political or religious controversy, the standard of scholarship has frequently been underwhelming. Despite these difficulties, bioethics burgeoned. A cynic might ascribe this accomplishment to the ample fertilization that the field received from a number of practical imperatives: absolving doctors of moral dilemmas, training medical students to behave, enabling hospital boards to trumpet their commitment to the highest ethical standards of care, providing sound bites for the mass media, and allowing politicians to cover their behinds by delegating controversial issues to ethics committees. But a kinder gloss is possible: decent people recognized that difficult moral problems arose in modern biomedicine, that these problems needed to be addressed, and that having some professional scholars trying to clarify these problems in some sort of systematic way might be helpful. While higher-caliber scholarship and a more robust methodology would be nice, in the meantime we make the most of what we have.

Moral philosophers have in the last couple of decades made many contributions that bear on the ethics of human transformation, and we must limit ourselves to a few mentions. Derek Parfits classic Reasons and Persons (1984) discussed many relevant normative issues.[45]In addition to personal identity and foundational ethical theory, this book treats population ethics, person-affecting moral principles, and duties to future generations. Although Parfits analysis takes place on an idealized level, his arguments elucidate many moral considerations that emerge within the transhumanist program.

Jonathan Glovers What Sort of People Should there Be? (1984) addressed technology-enabled human-transformation at a somewhat more concrete level, focusing especially on genetics and various technologies that could increase social transparency. Glover gave a clear and balanced analytic treatment of these issues that was well ahead of its time. His general conclusion is that

not just any aspect of present human nature is worth preserving. Rather it is especially those features which contribute to self-development and self-expression, to certain kinds of relationships, and to the development of our consciousness and understanding. And some of these features may be extended rather than threatened by technology.[46]

James Hughes has argued that biopolitics is emerging as a fundamental new dimension of political opinion. In Hughes model, biopolitics joins with the more familiar dimensions of cultural and economic politics, to form a three-dimensional opinion-space. We have already seen that in the early 90s, the extropians combined liberal cultural politics and laissez-fair economic politics with transhumanist biopolitics. In Citizen Cyborg (2004), Hughes sets forward what he terms "democratic transhumanism, " which mates transhumanist biopolitics with social democratic economic politics and liberal cultural politics.[68]He argues that we will achieve the best posthuman future when we ensure that technologies are safe, make them available to everyone, and respect the right of individuals to control their own bodies. The key difference between extropian transhumanism and democratic transhumanism is that the latter accords a much bigger role for government in regulating new technologies for safety and ensuring that the benefits will be available to all, not just a wealthy or tech-savvy elite.

In principle, transhumanism can be combined with a wide range of political and cultural views, and many such combinations are indeed represented, e.g. within the membership of the World Transhumanist Association. One combination that is not often found is the coupling of transhumanism to a culture-conservative outlook. Whether this is because of an irresolvable tension between the transformative agenda of transhumanism and the cultural conservatives preference for traditional arrangements is not clear. It could instead be because nobody has yet seriously attempted to develop such a position. It is possible to imagine how new technologies could be used to reinforce some culture-conservative values. For instance, a pharmaceutical that facilitated long-term pair bonding could help protect the traditional family. Developing ways of using our growing technological powers to help people realize widely held cultural or spiritual values in their lives would seem a worthwhile undertaking.

This is not, however, the route for which cultural conservatives have so far opted. Instead, they have gravitated towards transhumanisms opposite, bioconservatism, which opposes the use of technology to expand human capacities or to modify aspects of our biological nature. People drawn to bioconservatism come from groups that traditionally have had little in common. Right-wing religious conservatives and left-wing environmentalists and anti-globalists have found common causes, for example in their opposition to the genetic modification of humans.

The different strands of contemporary bioconservatism can be traced to a multifarious set of origins: ancient notions of taboo; the Greek concept of hubris; the Romanticist view of nature; certain religious (anti-humanistic) interpretations of the concept of human dignity and of a God-given natural order; the Luddite workers revolt against industrialization; Karl Marxs analysis of technology under capitalism; various Continental philosophers critiques of technology, technocracy, and the rationalistic mindset that accompanies modern technoscience; foes of the military-industrial complex and multinational corporations; and objectors to the consumerist rat-race. The proposed remedies have ranged from machine-smashing (the original Luddites), to communist revolution (Marx), to buying "organic ", to yoga (Jos Ortega y Gasset), but nowadays it commonly emanates in calls for national or international bans on various human enhancement technologies (Fukuyama, Annas, etc.).

Feminist writers have come down on both sides of the debate. Ecofeminists have suspected biotechnology, especially its use to reshape bodies or control reproduction, of being an extension of traditional patriarchal exploitation of women, or, alternatively, have seen it as a symptom of a control-obsessed, unemphatic, gadget-fixated, body-loathing mindset. Some have offered a kind of psychoanalysis of transhumanism, concluding that it represents an embarrassing rationalization of self-centered immaturity and social failure. But others have welcomed the libratory potential of biotechnology. Shulamith Firestone argued in the feminist classic The Dialectic of Sex (1971) that women will be fully liberated only when technology has freed them from having to incubate children.[69]Cyberfeminist Donna Haraway says that she would "rather be a cyborg than a goddess " and argues against the dualistic view that associates men with culture and technology and women with nature.[70]

Perhaps the most prominent bioconservative voice today is that of Leon Kass, chairman of President Bushs Council on Bioethics. Kass acknowledges an intellectual debt to three other distinguished bioconservatives: Protestant theologian Paul Ramsey, Christian apologist C. S. Lewis, and German-born philosopher-theologian Hans Jonas (who studied under Martin Heidegger).[71]Kasss concerns center on human dignity and the subtle ways in which our attempts to assert technological mastery over human nature could end up dehumanizing us by undermining various traditional "meanings " such as the meaning of the life cycle, the meaning of sex, the meaning of eating, and the meaning of work. Kass is well-known for his advocacy of "the wisdom of repugnance " (which echoes Hans Jonass "heuristics of fear "). While Kass stresses that a gut feeling of revulsion is not a moral argument, he nevertheless insists that the yuck factor merits our respectful attention:

In crucial cases repugnance is the emotional expression of deep wisdom, beyond reasons power to fully articulate we intuit and feel, immediately and without argument, the violation of things we rightfully hold dear To pollution and perversion, the fitting response can only be horror and revulsion; and conversely, generalized horror and revulsion are prima facie evidence of foulness and violation.[72]

Francis Fukuyama, another prominent bioconservative and member of the Presidents Council, has recently identified transhumanism as "the worlds most dangerous idea ".[73]For Fukuyama, however, the chief concern is not about the subtle undermining of meanings " but the prospect of violence and oppression. He argues that liberal democracy depends on the fact that all humans share an undefined "Factor X ", which grounds their equal dignity and rights. The use of enhancing technologies, he fears, could destroy Factor X.[74]

Bioethicists George Annas, Lori Andrews, and Rosario Isasi have proposed legislation to make inheritable genetic modification in humans a "crime against humanity ", like torture and genocide. Their rationale is similar to Fukuyamas:

The new species, or "posthuman, " will likely view the old "normal " humans as inferior, even savages, and fit for slavery or slaughter. The normals, on the other hand, may see the posthumans as a threat and if they can, may engage in a preemptive strike by killing the posthumans before they themselves are killed or enslaved by them. It is ultimately this predictable potential for genocide that makes species-altering experiments potential weapons of mass destruction, and makes the unaccountable genetic engineer a potential bioterrorist.[75]

There is some common ground between Annas et al. and the transhumanists: they agree that murder and enslavement, whether of humans by posthumans or the other way around, would be a moral atrocity and a crime. Transhumanists deny, however, that this is a likely consequence of germ-line therapy to enhance health, memory, longevity, or other similar traits in humans. If and when we develop the capability to create some singular entity that could potentially destroy the human race, such as a superintelligent machine, then we could indeed regard it as a crime against humanity to proceed without a thorough risk analysis and the installation of adequate safety features. As we saw in the previous section, the effort to understand and find ways to reduce existential risks has been a central preoccupation for some transhumanists, such as Eric Drexler, Nick Bostrom, and Eliezer Yudkowsky.

There are other commonalities between bioconservatives and transhumanists. Both agree that we face a realistic prospect that technology could be used to substantially transform the human condition in this century. Both agree that this imposes an obligation on the current generation to think hard about the practical and ethical implications. Both are concerned with medical risks of side-effects, of course, although bioconservatives are more worried that the technology might succeed than that it might fail. Both camps agree that technology in general and medicine in particular have a legitimate role to play, although bioconservatives tend to oppose many uses of medicine that go beyond therapy to enhancement. Both sides condemn the racist and coercive state-sponsored eugenics programs of the twentieth century. Bioconservatives draw attention to the possibility that subtle human values could get eroded by technological advances, and transhumanists should perhaps learn to be more sensitive to these concerns. On the other hand, transhumanists emphasize the enormous potential for genuine improvements in human well-being and human flourishing that are attainable only via technological transformation, and bioconservatives could try to be more appreciative of the possibility that we could realize great values by venturing beyond our current biological limitations.

The Transhumanist Declaration

(1) Humanity will be radically changed by technology in the future. We foresee the feasibility of redesigning the human condition, including such parameters as the inevitability of aging, limitations on human and artificial intellects, unchosen psychology, suffering, and our confinement to the planet earth.

(2) Systematic research should be put into understanding these coming developments and their long-term consequences.

(3) Transhumanists think that by being generally open and embracing of new technology we have a better chance of turning it to our advantage than if we try to ban or prohibit it.

(4) Transhumanists advocate the moral right for those who so wish to use technology to extend their mental and physical (including reproductive) capacities and to improve their control over their own lives. We seek personal growth beyond our current biological limitations.

(5) In planning for the future, it is mandatory to take into account the prospect of dramatic progress in technological capabilities. It would be tragic if the potential benefits failed to materialize because of technophobia and unnecessary prohibitions. On the other hand, it would also be tragic if intelligent life went extinct because of some disaster or war involving advanced technologies.

(6) We need to create forums where people can rationally debate what needs to be done, and a social order where responsible decisions can be implemented.

(7) Transhumanism advocates the well- being of all sentience (whether in artificial intellects, humans, posthumans, or non- human animals) and encompasses many principles of modern humanism. Transhumanism does not support any particular party, politician or political platform.

Annas, G., L. Andrews, and R. Isasi (2002), "Protecting the Endangered Human: Toward an International Treaty Prohibiting Cloning and Inheritable Alterations ", American Journal of Law and Medicine 28 (2&3):151-178.

Bacon, F. (1620), Novum Organum. Translated by R. L. Ellis and J. Spedding. Robertson, J. ed, The Philosophical Woeks of Francis Bacon, 1905. London: Routledge.

Bernal, J. D. (1969), The world, the flesh & the devil; an enquiry into the future of the three enemies of the rational soul. Bloomington: Indiana University Press.

Bostrom, N. (1998), "How Long Before Superintelligence? " International Journal of Futures Studies 2.

(2002), "Existential Risks: Analyzing Human Extinction Scenarios and Related Hazards ", Journal of Evolution and Technology 9.

(2002), "When Machines Outsmart Humans ", Futures 35 (7):759-764.

(2003), "Are You Living in a Computer Simulation? " Philosophical Quarterly 53 (211):243-255.

(2003), "Human Genetic Enhancements: A Transhumanist Perspective ", Journal of Value Inquiry 37 (4):493-506.

The Transhumanist FAQ: v 2.1. World Transhumanist Association 2003. http://transhumanism.org/index.php/WTA/faq/.

(2004), "Transhumanism - The World's Most Dangerous Idea? " Betterhumans 10/19/2004.

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Genetic Engineering – BiologyMad

Posted: June 17, 2016 at 4:55 am

Genetic Engineering

Genetic engineering, also known as recombinant DNA technology, means altering the genes in a living organism to produce a Genetically Modified Organism (GMO) with a new genotype. Various kinds of genetic modification are possible: inserting a foreign gene from one species into another, forming a transgenic organism; altering an existing gene so that its product is changed; or changing gene expression so that it is translated more often or not at all.

Genetic engineering is a very young discipline, and is only possible due to the development of techniques from the 1960s onwards. Watson and Crick have made these techniques possible from our greater understanding of DNA and how it functions following the discovery of its structure in 1953. Although the final goal of genetic engineering is usually the expression of a gene in a host, in fact most of the techniques and time in genetic engineering are spent isolating a gene and then cloning it. This table lists the techniques that we shall look at in detail.

1

cDNA

To make a DNA copy of mRNA

2

To cut DNA at specific points, making small fragments

3

DNA Ligase

To join DNA fragments together

4

Vectors

To carry DNA into cells and ensure replication

5

Plasmids

Common kind of vector

6

Gene Transfer

To deliver a gene to a living cells

7

Genetic Markers

To identify cells that have been transformed

8

To make exact copies of bacterial colonies on an agar plate

9

PCR

To amplify very small samples of DNA

10

DNA probes

To identify and label a piece of DNA containing a certain sequence

11

Shotgun *

To find a particular gene in a whole genome

12

Antisense genes *

To stop the expression of a gene in a cell

13

Gene Synthesis

To make a gene from scratch

14

Electrophoresis

To separate fragments of DNA

* Additional information that is not directly included in AS Biology. However it can help to consolidate other techniques.

Complementary DNA (cDNA) is DNA made from mRNA. This makes use of the enzyme reverse transcriptase, which does the reverse of transcription: it synthesises DNA from an RNA template. It is produced naturally by a group of viruses called the retroviruses (which include HIV), and it helps them to invade cells. In genetic engineering reverse transcriptase is used to make an artificial gene of cDNA as shown in this diagram.

Complementary DNA has helped to solve different problems in genetic engineering:

It makes genes much easier to find. There are some 70 000 genes in the human genome, and finding one gene out of this many is a very difficult (though not impossible) task. However a given cell only expresses a few genes, so only makes a few different kinds of mRNA molecule. For example the b cells of the pancreas make insulin, so make lots of mRNA molecules coding for insulin. This mRNA can be isolated from these cells and used to make cDNA of the insulin gene.

These are enzymes that cut DNA at specific sites. They are properly called restriction endonucleases because they cut the bonds in the middle of the polynucleotide chain. Some restriction enzymes cut straight across both chains, forming blunt ends, but most enzymes make a staggered cut in the two strands, forming sticky ends.

The cut ends are sticky because they have short stretches of single-stranded DNA with complementary sequences. These sticky ends will stick (or anneal) to another piece of DNA by complementary base pairing, but only if they have both been cut with the same restriction enzyme. Restriction enzymes are highly specific, and will only cut DNA at specific base sequences, 4-8 base pairs long, called recognition sequences.

Restriction enzymes are produced naturally by bacteria as a defence against viruses (they restrict viral growth), but they are enormously useful in genetic engineering for cutting DNA at precise places ("molecular scissors"). Short lengths of DNA cut out by restriction enzymes are called restriction fragments. There are thousands of different restriction enzymes known, with over a hundred different recognition sequences. Restriction enzymes are named after the bacteria species they came from, so EcoR1 is from E. coli strain R, and HindIII is from Haemophilis influenzae.

This enzyme repairs broken DNA by joining two nucleotides in a DNA strand. It is commonly used in genetic engineering to do the reverse of a restriction enzyme, i.e. to join together complementary restriction fragments.

The sticky ends allow two complementary restriction fragments to anneal, but only by weak hydrogen bonds, which can quite easily be broken, say by gentle heating. The backbone is still incomplete.

DNA ligase completes the DNA backbone by forming covalent bonds. Restriction enzymes and DNA ligase can therefore be used together to join lengths of DNA from different sources.

In biology a vector is something that carries things between species. For example the mosquito is a disease vector because it carries the malaria parasite into humans. In genetic engineering a vector is a length of DNA that carries the gene we want into a host cell. A vector is needed because a length of DNA containing a gene on its own wont actually do anything inside a host cell. Since it is not part of the cells normal genome it wont be replicated when the cell divides, it wont be expressed, and in fact it will probably be broken down pretty quickly. A vector gets round these problems by having these properties:

It is big enough to hold the gene we want (plus a few others), but not too big.

It is circular (or more accurately a closed loop), so that it is less likely to be broken down (particularly in prokaryotic cells where DNA is always circular).

It contains control sequences, such as a replication origin and a transcription promoter, so that the gene will be replicated, expressed, or incorporated into the cells normal genome.

It contain marker genes, so that cells containing the vector can be identified.

Many different vectors have been made for different purposes in genetic engineering by modifying naturally-occurring DNA molecules, and these are now available off the shelf. For example a cloning vector contains sequences that cause the gene to be copied (perhaps many times) inside a cell, but not expressed. An expression vector contains sequences causing the gene to be expressed inside a cell, preferably in response to an e
xternal stimulus, such as a particular chemical in the medium. Different kinds of vector are also available for different lengths of DNA insert:

Type of vector

Max length of DNA insert

10 kbp

Virus or phage

30 kbp

Bacterial Artificial Chromosome (BAC)

500 kbp

Plasmids are by far the most common kind of vector, so we shall look at how they are used in some detail. Plasmids are short circular bits of DNA found naturally in bacterial cells. A typical plasmid contains 3-5 genes and there are usually around 10 copies of a plasmid in a bacterial cell. Plasmids are copied separately from the main bacterial DNA when the cell divides, so the plasmid genes are passed on to all daughter cells. They are also used naturally for exchange of genes between bacterial cells (the nearest they get to sex), so bacterial cells will readily take up a plasmid. Because they are so small, they are easy to handle in a test tube, and foreign genes can quite easily be incorporated into them using restriction enzymes and DNA ligase.

One of the most common plasmids used is the R-plasmid (or pBR322). This plasmid contains a replication origin, several recognition sequences for different restriction enzymes (with names like PstI and EcoRI), and two marker genes, which confer resistance to different antibiotics (ampicillin and tetracycline).

The diagram below shows how DNA fragments can be incorporated into a plasmid using restriction and ligase enzymes. The restriction enzyme used here (PstI) cuts the plasmid in the middle of one of the markergenes (well see why this is useful later). The foreign DNA anneals with the plasmid and is joined covalently by DNA ligase to form a hybrid vector (in other words a mixture or hybrid of bacterial and foreign DNA). Several other products are also formed: some plasmids will simply re-anneal with themselves to re-form the original plasmid, and some DNA fragments will join together to form chains or circles. Theses different products cannot easily be separated, but it doesnt matter, as the marker genes can be used later to identify the correct hybrid vector.

Vectors containing the genes we want must be incorporated into living cells so that they can be replicated or expressed. The cells receiving the vector are called host cells, and once they have successfully incorporated the vector they are said to be transformed. Vectors are large molecules which do not readily cross cell membranes, so the membranes must be made permeable in some way. There are different ways of doing this depending on the type of host cell.

Heat Shock. Cells are incubated with the vector in a solution containing calcium ions at 0C. The temperature is then suddenly raised to about 40C. This heat shock causes some of the cells to take up the vector, though no one knows why. This works well for bacterial and animal cells.

Electroporation. Cells are subjected to a high-voltage pulse, which temporarily disrupts the membrane and allows the vector to enter the cell. This is the most efficient method of delivering genes to bacterial cells.

Viruses. The vector is first incorporated into a virus, which is then used to infect cells, carrying the foreign gene along with its own genetic material. Since viruses rely on getting their DNA into host cells for their survival they have evolved many successful methods, and so are an obvious choice for gene delivery. The virus must first be genetically engineered to make it safe, so that it cant reproduce itself or make toxins. Three viruses are commonly used:

1. Bacteriophages (or phages) are viruses that infect bacteria. They are a very effective way of delivering large genes into bacteria cells in culture.

2. Adenoviruses are human viruses that causes respiratory diseases including the common cold. Their genetic material is double-stranded DNA, and they are ideal for delivering genes to living patients in gene therapy. Their DNA is not incorporated into the hosts chromosomes, so it is not replicated, but their genes are expressed.

The adenovirus is genetically altered so that its coat proteins are not synthesised, so new virus particles cannot be assembled and the host cell is not killed.

3. Retroviruses are a group of human viruses that include HIV. They are enclosed in a lipid membrane and their genetic material is double-stranded RNA. On infection this RNA is copied to DNA and the DNA is incorporated into the hosts chromosome. This means that the foreign genes are replicated into every daughter cell.

After a certain time, the dormant DNA is switched on, and the genes are expressed in all the host cells.

Plant Tumours. This method has been used successfully to transform plant cells, which are perhaps the hardest to do. The gene is first inserted into the Ti plasmid of the soil bacterium Agrobacterium tumefaciens, and then plants are infected with the bacterium. The bacterium inserts the Ti plasmid into the plant cells' chromosomal DNA and causes a "crown gall" tumour. These tumour cells can be cultured in the laboratory and whole new plants grown from them by micropropagation. Every cell of these plants contains the foreign gene.

Gene Gun. This extraordinary technique fires microscopic gold particles coated with the foreign DNA at the cells using a compressed air gun. It is designed to overcome the problem of the strong cell wall in plant tissue, since the particles can penetrate the cell wall and the cell and nuclear membranes, and deliver the DNA to the nucleus, where it is sometimes expressed.

Micro-Injection. A cell is held on a pipette under a microscope and the foreign DNA is injected directly into the nucleus using an incredibly fine micro-pipette. This method is used where there are only a very few cells available, such as fertilised animal egg cells. In the rare successful cases the fertilised egg is implanted into the uterus of a surrogate mother and it will develop into a normal animal, with the DNA incorporated into the chromosomes of every cell.

Liposomes. Vectors can be encased in liposomes, which are small membrane vesicles (see module 1). The liposomes fuse with the cell membrane (and sometimes the nuclear membrane too), delivering the DNA into the cell. This works for many types of cell, but is particularly useful for delivering genes to cell in vivo (such as in gene therapy).

These are needed to identify cells that have successfully taken up a vector and so become transformed. With most of the techniques above less than 1% of the cells actually take up the vector, so a marker is needed to distinguish these cells from all the others. Well look at how to do this with bacterial host cells, as thats the most common technique.

A common marker, used in the R-plasmid, is a gene for resistance to an antibiotic such as tetracycline. Bacterial cells taking up this plasmid can make this gene product and so are resistant to this antibiotic. So if the cells are grown on a medium containing tetracycline all the normal untransformed cells, together with cells that ha
ve taken up DNA thats not in a plasmid (99%) will die. Only the 1% transformed cells will survive, and these can then be grown and cloned on another plate.

Replica plating is a simple technique for making an exact copy of an agar plate. A pad of sterile cloth the same size as the plate is pressed on the surface of an agar plate with bacteria growing on it. Some cells from each colony will stick to the cloth. If the cloth is then pressed onto a new agar plate, some cells will be deposited and colonies will grow in exactly the same positions on the new plate. This technique has a number of uses, but the most common use in genetic engineering is to help solve another problem in identifying transformed cells.

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Genetic Engineering - BiologyMad

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Genetic Engineering – The New York Times

Posted: at 4:55 am

Latest Articles

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By ANDREW POLLACK

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By AMY HARMON

Residents there heard a proposal Monday from an M.I.T. scientist to use genetically engineered mice to stop the spread of the tick-borne disease.

By AMY HARMON

A common bacterium contains molecules that target RNA, not DNA. If it can be harnessed for use in humans, the process may lead to new forms of bioengineering.

By CARL ZIMMER

The formal announcement of the plans, which leaked last month, seeks to raise $100 million this year. The total price tag could exceed $1 billion.

By ANDREW POLLACK

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By GINA KOLATA

Ritual, a start-up, is introducing a multivitamin that is vegan, mostly free of genetically engineered ingredients and tailored to todays diets.

By STEPHANIE STROM

The transaction, if consummated, would create an industry giant whose products include pain medications, genetically modified crops and pesticides.

By MICHAEL J. de la MERCED and CHAD BRAY

Without disclosing details, Monsanto said its board was reviewing a proposal that would create a giant with a combined annual revenue of $67 billion.

By MICHAEL J. de la MERCED

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By ANDREW POLLACK

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results are proof of false marketing claims.

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By HILLARY ROSNER

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By ANDREW POLLACK

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By NINA FEDOROFF and JOHN BLOCK

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By JENNIFER STEINHAUER and STEPHANIE STROM

A trial in the Florida Keys has been tentatively approved, but public comment must be assessed first by the agency.

By ANDREW POLLACK

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Genetic Engineering - The New York Times

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Genetic Engineering | Greenpeace International

Posted: at 4:55 am

While scientific progress on molecular biology has a great potential to increase our understanding of nature and provide new medical tools, it should not be used as justification to turn the environment into a giant genetic experiment by commercial interests. The biodiversity and environmental integrity of the world's food supply is too important to our survival to be put at risk. What's wrong with genetic engineering (GE)?

Genetic engineering enables scientists to create plants, animals and micro-organisms by manipulating genes in a way that does not occur naturally.

These genetically modified organisms (GMOs) can spread through nature and interbreed with natural organisms, thereby contaminating non 'GE' environments and future generations in an unforeseeable and uncontrollable way.

Their release is 'genetic pollution' and is a major threat because GMOs cannot be recalled once released into the environment.

Because of commercial interests, the public is being denied the right to know about GE ingredients in the food chain, and therefore losing the right to avoid them despite the presence of labelling laws in certain countries.

Biological diversity must be protected and respected as the global heritage of humankind, and one of our world's fundamental keys to survival. Governments are attempting to address the threat of GE with international regulations such as the Biosafety Protocol.

April 2010: Farmers, environmentalists and consumers from all over Spain demonstrate in Madrid under the slogan "GMO-free agriculture." They demand the Government to follow the example of countries like France, Germany or Austria, and ban the cultivation of GM maize in Spain.

GMOs should not be released into the environment since there is not an adequate scientific understanding of their impact on the environment and human health.

We advocate immediate interim measures such as labelling of GE ingredients, and the segregation of genetically engineered crops and seeds from conventional ones.

We also oppose all patents on plants, animals and humans, as well as patents on their genes. Life is not an industrial commodity. When we force life forms and our world's food supply to conform to human economic models rather than their natural ones, we do so at our own peril.

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Transhuman – Transformers Wiki – TFWiki.net

Posted: at 4:48 am

After Shockwave is critically injured protecting Sephie, she looks for a way to defend herself against the Autobots.

The Decepticons put the finishing touches on a stellar spanner built within the volcano their ship crashed in, and use it to contact Cybertron. They're overjoyed to get in touch with Heatwave, whom they thought may have perished in the Autobots' spanner experiments. They transmit the spanner schematics across.

A week later, a spanner has been constructed on Cybertron. The Decepticons, Cliffjumper, Professor Arkeville, Will, and Rick are there to greet the new ambassador Shockwave to Earth, along with his aide, Fistfight. Some of the Decepticons are picked for a troop exchange with Cybertron, however when they try to use the spanner again, an overload shuts it down, and they will need to recalibrate it to try again.

Rick goes to talk to Sephie, who missed Shockwave's arrival, but she has news for him of her ownshe's been offered a job by oil tycoon R.J. Blackrock. Sephie is not at her new job for long when she's called to Blackrock's office. Blackrock is looking forward to unveiling the company's new Mega-Rig next week, and wants Sephie to invite her Decepticon friends along in case the Autobots put in an appearance.

The Decepticons agree, and attend the unveiling, but Blackrock's speech is cut off by the arrival of a group of Autobots. The humans seek shelter, only to run into Seaspray. Seaspray recognizes Sephie and fires at her, but Shockwave protects the girl, taking the missile's impact. Shockwave is badly damaged, but not critically, and the Decepticons take him back to base for repairs. Sephie is particularly upset at his sacrifice. She makes contact with her mysterious Internet contact, Stormbringer99, who turns out to be Jetstorm. She meets him near Tucson, and he gives her a gift with which to "save her species".

Tailgate and his pet human Butch Witwicky are terrorizing the students of Franklin Burns High School when Sephie, now outfitted with golden electrical wiring covering her body, confronts them. Though Tailgate scoffs at the upstart human, Sephie is more than a match for him, blasting him until he retreats with Butch. The effort fries Sephie's wiring, and her clothing, but the grateful students lend her clothing. Grabbing Arkeville's cybernetic research, Sephie returns to Jetstorm and asks him for more help. Some time later, Sephie reveals her new upgrades to Rick and Will, who are duly horrified at her having replaced half her body with Cybertronian tech.

Elsewhere, Blackrock meets with Rodimus Prime and some other Autobots, and presents them with a gift of refined oil. Blackrock seeks weapons, which Goldbug regards as a bad idea, but Rodimus's interest is raised.

Sephie shows her enhancements to Starscream and Soundwave, who are just as worried as the others at what she's done. Starscream confines her to quarters until he and Arkeville can find some way to reverse the enhancements. She doesn't take it well. In her quarters, she's contacted on her oPhone by Blackrock, who claims the Autobots are forcing him to supply them with oil, and wants the Decepticons to arrange a trap. Sephie decides to go stop the Autobots herself. The Decepticons find out and prepare to go after her. Rick wants to go to, and Cliffjumper's talk of Nebulans gives them the idea to modify Fistfight into an exosuit for Rick.

Currently Sephie isn't in need of any help. Having downed Groove, she duplicates the abilities of his power chip rectifier and, introducing herself to Elita-One as "Emulator", uses Groove's fuel draining power and Tailgate's magnetic power to disable her too. Rick finds Sephie at Arizona Bay, looking to attack the Autobot base, and tries to talk her out of it. They're confronted by Rodimus, who distracts them while Wheeljack uses a microwave emitter to disable Sephie. Rick is blasted, and wakes up to see Wheeljack and Ratchet preparing to vivisect Sephie. Beachcomber detects a jamming field, which means the Decepticons are on their way.

Starscream's team arrives, but the Autobots have two hostages, not to mention a bunch of hidden Autobots who surprise and overwhelm the Decepticons. The prisoners are dutifully bound and a firing squad prepared, however Soundwave reveals he's not the one doing the jamming. As everyone looks up, a transport plane overhead drops off a massive robotic formBlackrock in his huge Centurion mech. While Blackrock fights the Autobots, Soundwave uses high-frequency vibrations to free himself, however he's immediately incapacitated by Blackrock, who views both Autobot and Decepticon as potential technology goldmines. Rick, meanwhile, leaves Fistfight so he can help Sephie. Though Rick intends them to flee, Sephie wants to help stop the Autobots and Blackrock. She first frees Cliffjumper, knowing he's pragmatic enough to free the other Decepticons before going after her, and then attacks the Centurion. With casualties mounting in the Autobot ranks, Rodimus sounds a retreat and they flee. Sephie uses Groove's fuel evaporating powers on the Centurion, and Blackrock ejects as it begins to go critical. Sephie is forced to take the giant high into the air before it explodes. Sephie survives the explosion using Elita-One's time-bubble power, returning to earth where she's hugged by a relieved Rick. Starscream wants to turn Blackrock over to the authorities, but he points out that they've trespassed on his land and destroyed his machinery, so they're the ones in the wrong. Also, he fires Sephie.

The Decepticons return to base, where Shockwave is repaired enough to be up and about, thanks to some hints from scans Starscream took of Sephie's cybernetic systems. Starscream offers Sephie a place in the Decepticon team, but she turns him down, deciding to strike out on her own.

In the Autobot base, Rodimus gives a speech to his discouraged troops, announcing double energon rations, and promising pay bonuses for anyone who snuffs a Decepticon or obtains technology or oil. Morale rises.

Jetstorm helps Sephie replace her blood with mech fluid. Sephie is unsure what she'll do next, but Jetstorm says she'll be a great hero and end the Great War. Also, he asks if she got Soundwave's talent, as there's something he needs her to find...

In a darkened room, Jetstorm and Demolishor communicate by hologram with a third party. They discuss the success of the Transhuman project, and their manipulation of the two Cybertronian factions. The meeting is interrupted by Star Saber, who is shocked to find Side Burn talking to a Decepticon and whatever Jetstorm is. He blasts Side Burn... but only reveals the fact that Side Burn isn't Cybertronian. Side Burn grabs Star Saber by the neck and knocks the Autobot offline with a surge of electricity, before proclaiming that the Underbase will be whole once again.

(Numbers indicate order of appearance.)

"Inverted vortex capacitor?" "Inverted vortex capacitor operational!" "Barium shield capacitors?" "Barium shield capacitors operational!" "Pipefor?" "Pipefor... wait. What's a pipefor?" "For blowin' exhaust, dude!"

"Huh. You talk exactly like you type." "I do not type."

"Butch, your temperature's going up. You want a new friend? I don't see its car... it's a stray! We can take it home and you can play together."

"Humanity plus, humanity squared, transhuman... Theyre all accurate. I'm what humanity needs to be the next step; a self-determined, self-defined, self-designed being. You can call me Emulator."

"Whoah, whoah... Somebody's got man
ufacturer issues. Scrappin' me ain't gonna make daddy Starscream love you, little girl, or whatever it is you're after. Oh for the luvva... now the fat kid's got power armor? This is gettin' outta hand."

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Drug-Gene Testing – Mayo Clinic Research

Posted: June 16, 2016 at 5:40 pm

Drug-gene testing is also called pharmacogenomics, or pharmacogenetics. All terms characterize the study of how your genes affect your bodys response to medications. The word pharmacogenomics is combined from the words pharmacology (the study of the uses and effects of medications) and genomics (the study of genes and their functions).

Your body has thousands of genes that you inherited from your parents. Genes determine which characteristics you have, such as eye color and blood type. Some genes are responsible for how your body processes medications. Pharmacogenomic tests look for changes or variants in these genes that may determine whether a medication could be an effective treatment for you or whether you could have side effects to a specific medication.

Patient Information: Pharmacogenomics Finding the Right Medication for You

Pharmacogenomic testing is one tool that can help your health care provider determine the best medication for you. Your health care provider also considers other factors such as your age, lifestyle, other medications you are taking and your overall health when choosing the right treatment for you.

The purpose of pharmacogenomic testing is to find out if a medication is right for you. A small blood or saliva sample can help determine:

The laboratory looks for changes or variants in one or more genes that can affect your response to certain medications.

Each person would need to have the same specific pharmacogenomic test only once because your genetic makeup does not change over time. However, you may need other pharmacogenomics tests if you take another medication. Each medication is associated with a different pharmacogenomics test. Keep track of all your test results and share them with your health care providers.

The need for pharmacogenomics testing is determined on an individual basis. If your pharmacogenomic test results suggest you may not have a good response to a medication, your family members may have a similar response. Mayo Clinic recommends you share this information with your family members. Your health care provider can also provide recommendations for family members who may benefit from having testing.

Current limitations of pharmacogenomics testing include:

The cost of pharmacogenomics testing varies depending on which test is ordered and your health insurance coverage. To help you determine test costs and coverage:

A federal law called the Genetic Information Nondiscrimination Act (GINA) generally makes it illegal for health insurance companies to discriminate against you based on your genetic information. This federal law does not protect you against genetic discrimination by life insurance, disability insurance or long-term care insurance companies. Some states have laws in this area.

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Internet Censorship in China – The New York Times

Posted: at 5:40 pm

Latest Articles

While trying to emphasize Chinas connectivity, a report by a state newspaper acknowledged the creeping pace of connections in the country.

By EDWARD WONG

American officials cite blocked websites and other limits on information as bad for foreign companies doing business in the vast market.

By PAUL MOZUR

During a presentation on digital security, the architect, Fang Binxing, was forced to use location-masking software to reach websites in South Korea.

By AUSTIN RAMZY

A draft law posted by a technology regulator said sites in the country would have to register domain names with local service providers.

By PAUL MOZUR

The unexpected defense of an outspoken real estate tycoon has exposed uneasiness about President Xi Jinpings calls for unquestioning public obedience.

By CHRIS BUCKLEY

A list of forbidden news topics reportedly issued by Chinas propaganda authorities offers a picture of their anxieties.

By DIDI KIRSTEN TATLOW

Officials from the top broadcast regulator have said that programs will soon be subject to the same censorship as regular TV shows, according to a report in The Beijing Times.

New regulations will forbid any foreign company from publishing online content in China without the governments consent.

By DAVID BARBOZA and PAUL MOZUR

The comparison, posted on YouTube, prompted warnings that the writer could be penalized under Chinese law, even though the site is blocked in China.

By DIDI KIRSTEN TATLOW

Many Western governments oppose use of the word multilateral, which is considered code for nations making the rules on how people get online and who has access to data.

By DAN LEVIN

The specific legal implications surrounding the question of free speech are vexing many Chinese who are following Pu Zhiqiangs plight.

By EDWARD WONG

The study by the American group Freedom House pointed to Chinas strengthening its Great Firewall system of censorship and its criminalizing some kinds of online speech.

By EDWARD WONG

The remarks, given at Tsinghua University in Beijing, underlined Facebooks eagerness to expand in China, where it remains blocked.

By OWEN GUO

The app displays an error message instead of news articles, possibly in an effort to avoid running afoul of Chinese censorship policies.

By PAUL MOZUR and KATIE BENNER

The Chinese Ministry of Public Security did not give details in announcing the move, but the accused have presumably been detained.

The government hopes to foster an Internet society that doesn't concern itself with politics or current affairs.

By MURONG XUECUN

In its growing Internet crackdown, Beijing has turned to an old ban on picking quarrels and provoking trouble, once limited to physical acts like handing out fliers.

By EDWARD WONG

China, which has some of the worlds tightest Internet restrictions, has released a draft of a bill that authorizes broad powers to control the flow of online information.

By AUSTIN RAMZY

The lead developer of Lantern, a censorship-evading tool, discusses how it works and how it has reacted tothe new measuresby Chinese Internet regulators.

By PATRICK BOEHLER

Businesses are growing increasingly frustrated by obstacles to Internet access, according to a survey by the European Union Chamber of Commerce in China.

By EDWARD WONG

While trying to emphasize Chinas connectivity, a report by a state newspaper acknowledged the creeping pace of connections in the country.

By EDWARD WONG

American officials cite blocked websites and other limits on information as bad for foreign companies doing business in the vast market.

By PAUL MOZUR

During a presentation on digital security, the architect, Fang Binxing, was forced to use location-masking software to reach websites in South Korea.

By AUSTIN RAMZY

A draft law posted by a technology regulator said sites in the country would have to register domain names with local service providers.

By PAUL MOZUR

The unexpected defense of an outspoken real estate tycoon has exposed uneasiness about President Xi Jinpings calls for unquestioning public obedience.

By CHRIS BUCKLEY

A list of forbidden news topics reportedly issued by Chinas propaganda authorities offers a picture of their anxieties.

By DIDI KIRSTEN TATLOW

Officials from the top broadcast regulator have said that programs will soon be subject to the same censorship as regular TV shows, according to a report in The Beijing Times.

New regulations will forbid any foreign company from publishing online content in China without the governments consent.

By DAVID BARBOZA and PAUL MOZUR

The comparison, posted on YouTube, prompted warnings that the writer could be penalized under Chinese law, even though the site is blocked in China.

By DIDI KIRSTEN TATLOW

Many Western governments oppose use of the word multilateral, which is considered code for nations making the rules on how people get online and who has access to data.

By DAN LEVIN

The specific legal implications surrounding the question of free speech are vexing many Chinese who are following Pu Zhiqiangs plight.

By EDWARD WONG

The study by the American group Freedom House pointed to Chinas strengthening its Great Firewall system of censorship and its criminalizing some kinds of online speech.

By EDWARD WONG

The remarks, given at Tsinghua University in Beijing, underlined Facebooks eagerness to expand in China, where it remains blocked.

By OWEN GUO

The app displays an error message instead of news articles, possibly in an effort to avoid running afoul of Chinese censorship policies.

By PAUL MOZUR and KATIE BENNER

The Chinese Ministry of Public Security did not give details in announcing the move, but the accused have presumably been detained.

The government hopes to foster an Internet society that doesn't concern itself with politics or current affairs.

By MURONG XUECUN

In its growing Internet crackdown, Beijing has turned to an old ban on picking quarrels and provoking trouble, once limited to physical acts like handing out fliers.

By EDWARD WONG

China, which has some of the worlds tightest Internet restrictions, has released a draft of a bill that authorizes broad powers to control the flow of online information.

By AUSTIN RAMZY

The lead developer of Lantern, a censorship-evading tool, discusses how it works and how it has reacted tothe new measuresby Chinese Internet regulators.

By PATRICK BOEHLER

Businesses are growing increasingly frustrated by obstacles to Internet access, according to a survey by the European Union Chamber of Commerce in China.

By EDWARD WONG

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Stormwatch (comics) – Wikipedia, the free encyclopedia

Posted: at 5:39 pm

Stormwatch is a fictional superhero team appearing in American comic books published by WildStorm, which later became an imprint of DC Comics. Created by Jim Lee, the team first appeared in Stormwatch #1 (March 1993). After WildStorm became an imprint of DC Comics, the stories were set in the DC Universe, and the group was depicted as a secretive team of superheroes who tackle dangerous missions while remaining unknown to the larger superhero community.

Stormwatch (run by a fictional United Nations) is overseen from a satellite by its director, the Weatherman. The Weatherman was Henry Bendix, who had cybernetic implants connected to his brain to better monitor the world situation and his Stormwatch teams in action. His field commander was Jackson King (also known as Battalion), an African-American telekinetic. Other founding members include Hellstrike (an Irish police officer who is an energy being), Winter (an ex-Russian Spetznaz officer and an energy absorber), Fuji (a young Japanese man, an energy being trapped in a containment suit) and Diva (a young Italian woman with sonic powers).

Stormwatch began in the comic book Stormwatch, published by Image Comics and owned by Jim Lee. Early writers of Stormwatch included Jim Lee, Brandon Choi, H. K. Proger and Ron Marz; early artists included Scott Clark, Brett Booth, Matt Broome and Renato Arlem.

Marz, who had worked on Marvel Comics' Silver Surfer and developed Hal Jordan's Green Lantern replacement Kyle Rayner at DC Comics, took over the writing while James Robinson was writing WildC.A.T.s. Robinson and Marz, directed by Jim Lee, intertwined the books' storylines over several months.

Around this time, two two-issue miniseries were published: Stormwatch Team One (written by James Robinson) and WildC.A.T.s Team One (written by Steven Seagle). In the intertwined miniseries, the groundwork for both teams was laid in the mid-1960s by a core group consisting of Saul Baxter (Lord Emp), Zealot, Majestic, John Colt (the template for Spartan), Backlash, a young Henry Bendix and Jackson King's father Isaiah, all of whom would be members of (or figure prominently in) the later Stormwatch and WildC.A.T.s teams. In this series "WildStorm", the publishing imprint name, was a code word used by the United States Government: "Wild" was extraterrestrial life-forms, and "Storm" was invading forces.

Robinson's WildC.A.T.s and Marz's Stormwatch culminated in the Wildstorm Rising crossover, during which both teams were disrupted; Stormwatch incurred casualties, and the WildC.A.T.s were believed dead. After WildStorm Rising, Alan Moore took over the writing of WildC.A.T.s. After a second imprint-wide crossover, Warren Ellis took over writing Stormwatch with #37 (July 1996).

Ellis' version of Stormwatch injected sexual and horror elements, thinly-disguised political commentary and criticism of the United States government into the stories. The art was toned down from the more-exaggerated 1990s style which dominated the early Image Comics, allowing readers to take the book more seriously. During this period Ellis used Stormwatch to introduce the concept of the Bleed, a space between parallel universes which later featured in Planetary and other comics set in the Wildstorm Universe. By the end of volume one Ellis made Henry Bendix a manipulative villain, as Grant Morrison did with The Chief in DC's Doom Patrol.

Ellis continued to write the book into Stormwatch volume 2, until the August 1998 WildC.A.T.s/Aliens crossover (written by Ellis) saw the Stormwatch team decimated by xenomorphs (the creatures from the Alien film series). Most of the Stormwatch characters Ellis had not created were killed off in this story. A group of Stormwatch survivors became the main cast of Ellis' new series, The Authority, including his characters Jenny Sparks, Jack Hawksmoor, Apollo, the Midnighter, Swift (who debuted in Stormwatch vol. 1 #28, written by Jeff Mariotte) and two new characters who were successors of the Engineer and the Doctor from Ellis' Change or Die storyline. Stormwatch volume 2 ended with a story, set after WildC.A.T.s/Aliens, in which the United Nations disbanded Stormwatch. The last scene, a conversation between former members of Stormwatch Black, introduced The Authority and promoted its first issue. Other survivors from the original team (including Battalion, Christine Trelane, and Flint) appeared in The Authority, and King and Trelane became central characters of The Monarchy.

In the 11th issue of Planetary, another Ellis series in the same fictional universe, a secret agent (John Stone, modelled after the James Bond films and Jim Steranko's Nick Fury, Agent of S.H.I.E.L.D. comics) works for a 1960s precursor of Stormwatch: S.T.O.R.M., its command center known as S.T.O.R.M. Watch.

In September 2002, Stormwatch was revived as Stormwatch: Team Achilles, written by Micah Ian Wright. The series followed a human UN troubleshooting team dealing with superhuman-related problems. The planned final issue (#24) was never published, although its script is available for download online.[1]

Stormwatch was one of several comic books restarted after Wildstorm Comics' WorldStorm event. This version was launched in November 2006 with writer Christos Gage and penciller Doug Mahnke.[2][not in citation given] The series ended after issue #12,[3] but resumed in August 2008 as part of the Worlds End event with issue #13.[4] In the new series several dead characters (Hellstrike, Fuji, Winter and Fahrenheit) were resurrected and reformed as the new version of Stormwatch Prime (now sponsored by the United States), and a separate branch office Stormwatch: P.H.D. (Post-Human Division) was opened in New York.[5]

DC Comics announced in June 2011 that the team would be incorporated into the DC Universe in a new series, written by Paul Cornell and drawn by Miguel Sepulveda, as part of the September 2011 relaunch of its comics.[6]Peter Milligan took over the book in issue nine after leaving Justice League Dark with issue eight.[7]

This Stormwatch, an organization which has protected Earth from alien threats since the Dark Ages, is commanded by a group known as the Shadow Cabinet:[8] a four-member group of Shadow Lords[9] referred to as "the dead", and represented by an entity which can negate the group's powers and is aware of their secrets (except Harry's).[10] Rejecting the title "superheroes", Stormwatch Jack Hawksmoor, Apollo, Midnighter, Jenny Quantum, the Engineer, the Martian Manhunter (who left the team after wiping everyone's memory of him),[9] and three new characters: Adam One (an immortal born during the Big Bang,[11] who was later revealed to be Merlin),[12] Emma Rice,[13] the Projectionist (who controls the mass media) and Harry Tanner, the Eminence of Blades (the power to lie to anyone and be believed)[14] exist in secret and consider themselves professional soldiers. Their base is a hijacked Daemonite spaceship in Hyperspace,[15] later upgraded into the Carrier.[16]

Jim Starlin wrote Stormwatch with #19, erasing the team's history as a 1,000-year-old organization and restarting its history again. Apollo and Midnighter were returned to their original costumes as the core of a new Stormwatch team with the Engineer, Hellstrike, the Weird and new characters Jenny Soul, the Forecaster, and Force.[17] After Starlin's run ended with #29, Sterling Gates wrote the series' 30th and final issue which restored the previous version of the team.[18] The team then appeared in The New 52: Futures End weekly limited series.[19]

Ellis' run on Stormwatch was collected into five trade paperbacks:

Stormwatch: Team Achilles was collected into two trade paperbacks:

Stormwatch: Post-Human Divison was collected into four trade paperbacks:

The New 52 version of Stormwatch was collected into four trade paperbacks:

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Genetics & Medicine – Site Guide – NCBI

Posted: June 14, 2016 at 4:40 pm

Bookshelf

A collection of biomedical books that can be searched directly or from linked data in other NCBI databases. The collection includes biomedical textbooks, other scientific titles, genetic resources such as GeneReviews, and NCBI help manuals.

A resource to provide a public, tracked record of reported relationships between human variation and observed health status with supporting evidence. Related information intheNIH Genetic Testing Registry (GTR),MedGen,Gene,OMIM,PubMedand other sources is accessible through hyperlinks on the records.

A registry and results database of publicly- and privately-supported clinical studies of human participants conducted around the world.

An archive and distribution center for the description and results of studies which investigate the interaction of genotype and phenotype. These studies include genome-wide association (GWAS), medical resequencing, molecular diagnostic assays, as well as association between genotype and non-clinical traits.

An open, publicly accessible platform where the HLA community can submit, edit, view, and exchange data related to the human major histocompatibility complex. It consists of an interactive Alignment Viewer for HLA and related genes, an MHC microsatellite database, a sequence interpretation site for Sequencing Based Typing (SBT), and a Primer/Probe database.

A searchable database of genes, focusing on genomes that have been completely sequenced and that have an active research community to contribute gene-specific data. Information includes nomenclature, chromosomal localization, gene products and their attributes (e.g., protein interactions), associated markers, phenotypes, interactions, and links to citations, sequences, variation details, maps, expression reports, homologs, protein domain content, and external databases.

A collection of expert-authored, peer-reviewed disease descriptions on the NCBI Bookshelf that apply genetic testing to the diagnosis, management, and genetic counseling of patients and families with specific inherited conditions.

Summaries of information for selected genetic disorders with discussions of the underlying mutation(s) and clinical features, as well as links to related databases and organizations.

A voluntary registry of genetic tests and laboratories, with detailed information about the tests such as what is measured and analytic and clinical validity. GTR also is a nexus for information about genetic conditions and provides context-specific links to a variety of resources, including practice guidelines, published literature, and genetic data/information. The initial scope of GTR includes single gene tests for Mendelian disorders, as well as arrays, panels and pharmacogenetic tests.

A database of known interactions of HIV-1 proteins with proteins from human hosts. It provides annotated bibliographies of published reports of protein interactions, with links to the corresponding PubMed records and sequence data.

A compilation of data from the NIAID Influenza Genome Sequencing Project and GenBank. It provides tools for flu sequence analysis, annotation and submission to GenBank. This resource also has links to other flu sequence resources, and publications and general information about flu viruses.

A portal to information about medical genetics. MedGen includes term lists from multiple sources and organizes them into concept groupings and hierarchies. Links are also provided to information related to those concepts in the NIH Genetic Testing Registry (GTR), ClinVar,Gene, OMIM, PubMed, and other sources.

A project involving the collection and analysis of bacterial pathogen genomic sequences originating from food, environmental and patient isolates. Currently, an automated pipeline clusters and identifies sequences supplied primarily by public health laboratories to assist in the investigation of foodborne disease outbreaks and discover potential sources of food contamination.

A database of human genes and genetic disorders. NCBI maintains current content and continues to support its searching and integration with other NCBI databases. However, OMIM now has a new home at omim.org, and users are directed to this site for full record displays.

A database of citations and abstracts for biomedical literature from MEDLINE and additional life science journals. Links are provided when full text versions of the articles are available via PubMed Central (described below) or other websites.

A digital archive of full-text biomedical and life sciences journal literature, including clinical medicine and public health.

A collection of clinical effectiveness reviews and other resources to help consumers and clinicians use and understand clinical research results. These are drawn from the NCBI Bookshelf and PubMed, including published systematic reviews from organizations such as the Agency for Health Care Research and Quality, The Cochrane Collaboration, and others (see complete listing). Links to full text articles are provided when available.

A collection of resources specifically designed to support the research of retroviruses, including a genotyping tool that uses the BLAST algorithm to identify the genotype of a query sequence; an alignment tool for global alignment of multiple sequences; an HIV-1 automatic sequence annotation tool; and annotated maps of numerous retroviruses viewable in GenBank, FASTA, and graphic formats, with links to associated sequence records.

A summary of data for the SARS coronavirus (CoV), including links to the most recent sequence data and publications, links to other SARS related resources, and a pre-computed alignment of genome sequences from various isolates.

An extension of the Influenza Virus Resource to other organisms, providing an interface to download sequence sets of selected viruses, analysis tools, including virus-specific BLAST pages, and genome annotation pipelines.

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Genetic Engineering: What is Genetic Engineering?

Posted: June 12, 2016 at 12:39 am

Written by Patrick Dixon

Futurist Keynote Speaker: Posts, Slides, Videos - Biotechnology, Genetics, Gene Therapy, Stem Cells

Genetic engineering is the alteration of genetic code by artificial means, and is therefore different from traditional selective breeding.

Genetic engineering examples include taking the gene that programs poison in the tail of a scorpion, and combining it with a cabbage. These genetically modified cabbages kill caterpillers because they have learned to grow scorpion poison (insecticide) in their sap.

Genetic engineering also includes insertion of human genes into sheep so that they secrete alpha-1 antitrypsin in their milk - a useful substance in treating some cases of lung disease.

Genetic engineering has created a chicken with four legs and no wings.

Genetic engineering has created a goat with spider genes that creates "silk" in its milk.

Genetic engineering works because there is one language of life: human genes work in bacteria, monkey genes work in mice and earthworms. Tree genes work in bananas and frog genes work in rice. There is no limit in theory to the potential of genetic engineering.

Genetic engineering has given us the power to alter the very basis of life on earth.

Genetic engineering has been said to be no different than ancient breeding methods but this is untrue. For a start, breeding or cross-breeding, or in-breeding (for example to make pedigree dogs) all work by using the same species. In contrast genetic engineering allows us to combine fish, mouse, human and insect genes in the same person or animal.

Genetic engineering therefore has few limits - except our imagination, and our moral or ethical code.

Genetic engineering makes the whole digital revolution look nothing. Digital technology changes what we do. Genetic engineering has the power to change who we are.

Human cloning is a type of genetic engineering, but is not the same as true genetic manipulation. In human cloning, the aim is to duplicate the genes of an existing person so that an identical set is inside a human egg. The result is intended to be a cloned twin, perhaps of a dead child. Genetic engineering in its fullest form would result in the child produced having unique genes - as a result of laboratory interference, and therefore the child will not be an identikit twin.

Genetic engineering could create crops that grow in desert heat, or without fertiliser. Genetic engineering could make bananas or other fruit which contain vaccines or other medical products.

Genetic engineering will alter the basis of life on earth - permanently - unless controlled. This could happen if - say - mutant viruses, or bacteria, or fish or reptiles are released into the general environment.

READ FREE BOOK on Genetic Engineering - by Patrick Dixon, author of 16 books and creator of this website - read now: Chapters 1 and 2 explain basics in way which is easy to understand.

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Genetic Engineering: What is Genetic Engineering?

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