Page 1,873«..1020..1,8721,8731,8741,875..1,8801,890..»

Category Archives: Transhuman News

Reputable Geneticist and Research Scientist, Luigi Boccuto, MD, will be Presented in The Leading Physicians of the … – PR NewsChannel (press…

Posted: April 25, 2017 at 4:37 am

The International Association of HealthCare Professionals is pleased to welcome Dr. Luigi Boccuto, MD, to their prestigious organization with his upcoming publication in The Leading Physicians of the World. Dr. Luigi Boccuto is a highly trained geneticist and research scientist with an extensive expertise in all facets of his work, especially human genetics and molecular studies. He holds over 15 years of experience in his field and is currently an Assistant Research Scientist at the JC Self Research Institute of the Greenwood Genetic Center in Greenwood, South Carolina.

Dr. Boccuto graduated with his Medical Degree in 2002 from the Universit Cattolica del Sacro Cuore in Rome, Italy. Following his graduation, he completed specialized training in medical genetics within the same institution, and trained for years under Prof. Neri with a focus on hereditary cancer, overgrowth syndromes, and intellectual disability syndromes.

Dr. Boccuto is renowned for his important research in the field of medical genetics, focused on the study of the genetic causes of autism, ID, and conditions with segmental or generalized overgrowth. He remains a member of the American Society of Human Genetics, is the recipient of numerous awards and recognitions, and has been published extensively for his important research. He attributes his success to his life long quest for learning, problem solving, and desire to better the health of human beings. In his free time, Dr. Boccuto enjoys playing soccer, photography, traveling, and reading.

View Dr. Luigi Boccutos Profile Here: https://www.findatopdoc.com/doctor/8138568-Luigi-Boccuto-Geneticist-Greenwood-South-Carolina-29646

Learn more about Dr. Boccuto here:

http://www.ggc.org/ and be sure to read his upcoming publication in The Leading Physicians of the World.

About FindaTopDoc.com

FindaTopDoc.com is a hub for all things medicine, featuring detailed descriptions of medical professionals across all areas of expertise, and information on thousands of healthcare topics. Each month, millions of patients use FindaTopDoc to find a doctor nearby and instantly book an appointment online or create a review. FindaTopDoc.com features each doctors full professional biography highlighting their achievements, experience, patient reviews and areas of expertise. A leading provider of valuable health information that helps empower patient and doctor alike, FindaTopDoc enables readers to live a happier and healthier life. For more information about FindaTopDoc, visit http://www.findatopdoc.com

View original post here:
Reputable Geneticist and Research Scientist, Luigi Boccuto, MD, will be Presented in The Leading Physicians of the ... - PR NewsChannel (press...

Posted in Human Genetics | Comments Off on Reputable Geneticist and Research Scientist, Luigi Boccuto, MD, will be Presented in The Leading Physicians of the … – PR NewsChannel (press…

DNA Day teaches about science and scientists – News & Observer

Posted: at 4:37 am


News & Observer
DNA Day teaches about science and scientists
News & Observer
Visibility is a key goal of North Carolina DNA Day, a science outreach event that commemorates the discovery of the double helical structure of DNA and the completion of the human genome sequence. Research shows that when you ask a child to draw a ...

Original post:
DNA Day teaches about science and scientists - News & Observer

Posted in DNA | Comments Off on DNA Day teaches about science and scientists – News & Observer

Pa. lawmakers consider stronger laws for post-conviction DNA … – FOX43.com

Posted: at 4:37 am

Please enable Javascript to watch this video

HARRISBURG, Pa. -- Anthony Wright describes the day he was released from prison after 25 years "the greatest day of my life." He wants to ensure no one has to experience what he had to endure ever again.

Wright, of Philadelphia, plead guilty in 1991 of a crime he did not commit. Released from prison lat August, Wright, along with advocates from the Pennsylvania Innocence Project, are pushing for new legislation which would strengthen the state's post-conviction relief laws, expanding access to DNA testing for those already convicted of crimes.

"What happened to me 25 years ago changed my life," Wright said. "I'm not the first person this has happened to and I guarantee it won't be the last. We want to stop it so it doesn't happen to nobody else."

On Monday, Wright was the main witness testifying for the legislation, which is expected to have competing bills in the State House and Senate. State Representative Tedd Nesbit (R-Mercer) will sponsor the yet-to-be-assigned house bill, while Republican Judiciary Committee Chairman Stewart Greenleaf will author the Senate's bill.

In 1991, Wright pleaded guilty to rape and first-degree murder of Louise Talley. In 2005, he petitioned to test for DNA samples in the rape kit to help exonerate him. However, Wright was ineligible for DNA testing due to a state law which bars the retesting of DNA samples for those found guilty of crimes. Wright's push for a new trial reached the Supreme Court, which eventually led to a new hearing. In the new trial, DNA evidence excluded Wright as Talley's rapist, instead identifying Ronnie Byrd, a man who passed away in 2013.

In August 2016, Wright was found not guilty of raping and murdering Louise Talley, and was set free.

"It's the best evidence in the world," Wright says of DNA testing. "It says two things: Guiilty or not guilty. There's no in between."

At Monday's Senate Judiciary hearing, hosted by legislation sponsor Sen. Greenleaf, the proposed bill seemed to get bipartisan support.

"If there is DNA available, for God sakes, for the victim too, because no one benefits from an innocent person being in prison," said Senator Daylin Leach (D-Montgomery).

However, prosecutors from the District Attorneys Association wonder if allowing DNA testing for everyone would "open the flood gates" for everyone to challenge their conviction.

Dauphin County District Attorney Ed Marsico admits the current law needs tweaking, mostly because of the changes and advancements in science and technology as it relates to DNA.

"There just needs to be a legitimate avenue for it," Marsico said. "We are in favor of DNA testing in proper cases."

One area which he feels could be the first to see tweaks is a state law which states a Post-Conviction Relief Hearing can only be filed within 60 days after new evidence was discovered.

Senator Art Haywood, a Democrat from Philadelphia County, asked Marsico during the hearing, "What's the number of cases that is too many to make sure we have one innocent freed?"

Marsico responded, "I don't think its any particular number but we have to be cognizant. I don't want a guy who had a retail theft 30 years ago taxing (District Attorneys) when there's nothing out there that would lead to innocence."

40.273191 -76.886701

See the rest here:
Pa. lawmakers consider stronger laws for post-conviction DNA ... - FOX43.com

Posted in DNA | Comments Off on Pa. lawmakers consider stronger laws for post-conviction DNA … – FOX43.com

Indiana to take DNA sample from every person arrested for felony – nwitimes.com

Posted: at 4:37 am

INDIANAPOLIS Indiana law enforcement is entering a brave new world where police can obtain and test any Hoosier's DNA profile against crime scene evidence, so long as a prosecutor can show the person probably committed a felony.

Republican Gov. Eric Holcomb on Friday signed into law Senate Enrolled Act 322 requiring police to take a cheek swab DNA sample from every person arrested for a felony, starting in 2018.

Currently, only individuals convicted of felonies have their DNA records permanently entered into a state police database.

State Sen. Erin Houchin, R-Salem, the sponsor of the new law, said she expects police will catch more criminals once they have a bigger pool of DNA records to check against blood, fluids and other detritus gathered at crime scenes.

She also refused to rule out someday expanding the DNA collection mandate to include those arrested for misdemeanors or traffic infractions.

"DNA profiling is an accurate, widely used tool that will help law enforcement solve crimes and convict those who are responsible," Houchin said.

The new law provides that an individual's DNA sample only will be added to the state's database after a judge affirms that police had probable cause to arrest the person, which means it's more likely than not the person committed the crime he or she is accused of.

That's a significantly lower standard than the guilt beyond a reasonable doubt required for conviction.

If prosecutors are unable to convict, the law establishes a process for the person to request his or her DNA be expunged from the state database.

However, the Indiana Code also provides that if the record is not deleted as requested, that oversight does not invalidate any future arrest or conviction based on DNA evidence that shouldn't be in the database.

State Sen. Mike Young, R-Indianapolis, opposed the measure because he said it runs afoul of 4th Amendment protections against illegal police searches because an arrestee's DNA record will be used for investigatory purposes, not just identification.

"This is no different than the government coming in your house looking for evidence to see if there's anything laying around that they might be able to put together to find out you committed another crime. There is no difference," Young said.

"Why can't we just do it the right way and get a warrant?" he asked.

The law was approved 36-13 by the Senate and 84-13 in the House. Both chambers are Republican-controlled.

State Sen. Eddie Melton, D-Merrillville, and state Sen. Lonnie Randolph, D-East Chicago, were the only Northwest Indiana lawmakers to vote against the proposal.

Read the original here:
Indiana to take DNA sample from every person arrested for felony - nwitimes.com

Posted in DNA | Comments Off on Indiana to take DNA sample from every person arrested for felony – nwitimes.com

Frozen in Time: DNA May ID Sailors Looking for Northwest Passage … – Live Science

Posted: at 4:37 am

A sonar image showing the ill-fated HMS Erebus shipwreck.

Scientists have extracted DNA from the skeletal remains of several 19th-century sailors who died during the ill-fated Franklin Expedition, whose goal was to navigate the fabled Northwest Passage.

With a new genetic database of 24 expedition members, researchers hope they'll be able to identify some of the bodies scattered in the Canadian Arctic, 170 years after one of the worst disasters in the history of polar exploration.

The results were published April 20 in the Journal of Archaeological Science: Reports.

Led by Sir John Franklin, a British Royal Navy captain, the 129-member crew embarked in 1845 in search of a sea route that would link the Atlantic and Pacific oceans. The sailors were doomed after their ships became trapped in thick sea ice in the Canadian Arctic Archipelago in 1846. [In Photos: Arctic Shipwreck Solves 170-Year-Old Mystery]

The last communication, a short note from April 25, 1848, indicated that the surviving men were abandoning their ships the HMS Erebus and HMS Terror just off King William Island and embarking on a harsh journey south toward a trading post on the mainland. None of them seems to have made it even a fifth of the way there.

Over more than a century, search parties and scientists have discovered the remains of several Franklin sailors in boats and makeshift campsites scattered along this route. The bones bear scars of diseases like scurvy. Some even have the signatures of cannibalism, according to one recent study that confirmed the 19th-century reports of Inuit witnesses who had described piles of fractured human bones. Several artifacts from the HMS Erebus, including a medicine bottle and tunic buttons, as well as the ship's bronze bell, have also been uncovered.

In the latest look at the array of bones, a team led by Douglas Stenton of Nunavut's Department of Culture and Heritage, a territory in northern Canada, conducted the first genetic tests on members of the expedition who died following the desertion of the ships.

Stenton and his colleagues were able to get DNA from 37 bone and tooth samples found at eight different sites around King William Island, and they established the presence of at least 24 different members of the expedition. Twenty-one of these individuals had been found at locations around Canada's Erebus Bay, "confirming it as a location of some importance following the desertion of Erebus and Terror," Stenton told Live Science.

The researchers say their results offer a more accurate count of the number of expedition members who died at different locations. A few of the early fatalities were buried at Beechey Island and their frozen remains, which were exhumed by archaeologists in the 1980s, were eerily well-preserved. The bones of the sailors who died after abandoning the ships, however, were much more scattered, dispersed by animal scavenging and human activity.

Stenton said that, in one case, bones from the same individual were found at two different sites about a mile (1.7 kilometers) from each other. The researchers think that an 1879 search party most likely found some of the bones, and then carried them to the new site and reburied them.

Stenton and colleagues hope they will eventually be able to use the database to identify the crew members and better reconstruct what happened in the final months of the expedition.

"We have been in touch with several descendants who have expressed interest in participating in further research," Stenton said. "We hope that the publication of our initial study will encourage other descendants to also consider participating."

Four samples in the study were identified as female, which doesn't fit with the picture of an all-male expedition crew. The authors ruled out the possibility that these samples came from Inuit women because the genetic and archaeological evidence associated with these four individuals also suggests they were European. [Tales of the 9 Craziest Ocean Voyages]

"We were surprised by the results for those samples because in planning the analysis it hadn't occurred to us that there might have been women on board," Stenton told Live Science.

Stenton and his colleagues think the most likely explanation for this discrepancy is that ancient DNA studies commonly fail to amplify the Y chromosome (the male sex chromosome) due to insufficient quantity or quality of DNA, which can result in false female identifications of the dead. However, the researchers noted that it wasn't unheard of for women to serve in disguise in the Royal Navy.

"Some of these women were smuggled onboard [the] ship, and others disguised themselves as men and worked alongside the crew for months or years before being detected or intentionally revealing themselves to be female," the authors wrote.

They cited cases such as Mary Anne Talbot, who served on two Navy ships during the Napoleonic wars of the 18th century before being found out after being wounded. Unfortunately, Stenton said he doesn't think it will be possible to definitively say whether the four Franklin samples are really just false results, but his team concluded that it would have been very unlikely for so many women to be serving secretly on this voyage.

Original article on Live Science.

The rest is here:
Frozen in Time: DNA May ID Sailors Looking for Northwest Passage ... - Live Science

Posted in DNA | Comments Off on Frozen in Time: DNA May ID Sailors Looking for Northwest Passage … – Live Science

Swabbing a car door handle in a public lot to collect DNA is a Fourth Amendment trespass search – Washington Post

Posted: at 4:37 am

In United States v. Jones, 132 S.Ct. 945 (2012), the Supreme Court added a second test for what government action counts as a Fourth Amendment search. Since the 1970s, the Supreme Court had held that the government commits a search when it violates a persons reasonable expectation of privacy. Jones added that the government also commits a search when it trespasses on to a persons persons, houses, papers, and effects. As I explained in an article responding to Jones, it is hardly clear what kind of trespass test Jones adopts. Although Jones purports to restore a preexisting trespass test, no trespass test existed that the court could restore. As a result, the significance of Jones hinges on just what kind of trespass test courts interpret Jones to have adopted.

In light of that uncertainty, I was fascinated by a new decision, Schmidt v. Stassi, from the Eastern District of Louisiana last week. Michael Schmidt is a suspect in the 1997 murder of Eugenie Boisfontaine. You may have heard of the case, as the investigation is the subject of the Discovery Channel TV show Killing Fields. Investigators wanted to get a DNA sample from Schmidt, so they followed his car. When Schmidt drove to a local strip mall, parked and went inside a store, an agent used a cotton swab to wipe the exterior door handle on Schmidts Hummer to collect a DNA sample. Schmidt sued the officers, claiming that swabbing his car door handle was an unlawful Fourth Amendment search.

In the new decision, Judge Lance M. Africk holds that collecting the DNA from the door handle using the cotton swab was a Fourth Amendment search because it trespassed on to the car. From the opinion:

Here, the search involved the physical touching of Schmidts Hummer in a public parking lot. The search, however, did not damage the Hummer in any way. Accordingly, this Court has to make two determinations when evaluating whether a Fourth Amendment search occurred:

Does the trespass-trigger for Fourth Amendment coverage extend to a trespass to chattels? If so, was the physical touching a trespass to chattels even though the touching did not harm or otherwise affect the Hummer?

Joneswhich addressed a trespass against a carsettles that a trespass to chattles can constitute a Fourth Amendment search regardless of whether there is a reasonable expectation of privacy. See 565 U.S. at 410 (observing that officers trespassorily inserted the GPS tracker on the Jeep); see also id. at 419 & n.2 (Alito, J., concurring) (implying Court was concluding that search was a trespass to chattles). Thus, just as a trespass to land can constitute a Fourth Amendment search, a trespass to chattles may as well. See, e.g., United States v. Ackerman, 831 F.3d 1292, 1307-08 (10th Cir. 2016). And there is no question that an automobileunlike an open fieldis protected by the Fourth Amendment: an automobile is an effect as that term is used in the Fourth Amendment. Jones, 565 U.S. at 404.4

But was this a trespass to chattles? That is a trickier issue. As Justice Alitos Jones concurrence explained, the elements of the tort have changed since the founding. At common law, a suit for trespass to chattels could be maintained if there was a violation of the dignitary interest in the inviolability of chattels. 565 U.S. at 419 & n.2 (Alito, J., concurring) (internal quotation marks omitted). Meanwhile, today there must be some actual damage to the chattel before the action can be maintained. Id. (internal quotation marks omitted). So the choice of a particular understanding of trespass can be outcome determinative when applying Jones if a search does not damage or otherwise affect a particular chattel.

The Court concludes that it should follow the view that an officer need not cause damage before committing a trespass to chattels. Not only is that the view of the Second Restatement of Torts, see Restatement (Second) of Torts 217,5 but it also has the added advantage of not making the scope of the Fourth Amendment turn on whether someone scratches the paint.

The officers argued that Schmidt had abandoned his DNA by leaving it out in public for anyone to collect, analogizing the DNA to the trash left by the side of the road in California v. Greenwood. The court reasoned that Greenwood is inapplicable because the facts here involved a trespass:

[W]hatever the constitutionality of searching Schmidts curbside garbage for his abandoned DNA (a question on which the Court expresses no opinion), the officers argument that they may trespass to acquire abandoned property is not viable post-Jardines. See 133 S. Ct. at 1417 (That the officers learned what they learned only by physically intruding on [defendants] property to gather evidence is enough to establish that a search occurred.).

The Court concludes that the undisputed facts of this case establish that the officers committed a trespass to chattels when they swabbed Schmidts Hummer. Under Jones that trespass also constituted a Fourth Amendment search. Thus, Schmidt is entitled to partial summary judgment in that the swabbing constituted a search under the Fourth Amendment.

The opinion then stresses that given the present procedural posturethe parties addressed only the threshold issue of whether the swabbing was a Fourth Amendment searchthe Court expresses no opinion on whether the search was reasonable. Instead, Africk concludes that qualified immunity applies either way because the law is unsettled:

[T]he law is simply too unsettled after Jones for the Court to conclude that it is beyond debate, Ashcroft v. al-Kidd, 563 U.S. 731, 741 (2011), that the officers performed a Fourth Amendment search. Neither Jones nor Jardines is precise as to the body of property law this Court is supposed to follow when applying Joness trespass test. That unanswered question at the time of the swabbing would permit a reasonable officer to conclude that the swabbing did not constitute a Fourth Amendment search.

For example, a reasonable officer could have concludedjust as the Supreme Court has in the Fifth Amendment contextthat Jones-triggering trespasses are determined by reference to existing rules or understandings that stem from an independent source such as state law. Georgia v. Randolph, 547 U.S. 103, 144 (2006) (Scalia, J., dissenting). A reasonable officer could then pivot from that understanding of the Fourth Amendment, and conclude that because the brief, harmless, nearly imperceptible touching would not constitute an actionable trespass under certain understandings of modern tort law, see 565 U.S. at 419 & n.2 (Alito, J., concurring), it did not constitute a Jones-triggering trespass. Therefore qualified immunity is proper: an officer should not be denied qualified immunity simply because he or she looked to what an actionable trespass was as opposed to the more technical definition of a trespass.

Notably, the idea here is that collecting the DNA was a search because it interfered with Schmidts rights in the car, not in the DNA itself. Thats different from the reasonable-expectation-of-privacy cases on collecting DNA, which generally focus on the potential privacy invasion in the testing of the DNA sample to reveal sensitive information.

For related issues, see the petition for certiorari I filed in Arzola v. Massachusetts in 2015, together with the states brief in opposition and our reply brief. The Supreme Court denied the petition in Arzola, but I think its a useful starting point to see how the trespass framework may change Fourth Amendment rights in the context of DNA collection and analysis.

Read more:
Swabbing a car door handle in a public lot to collect DNA is a Fourth Amendment trespass search - Washington Post

Posted in DNA | Comments Off on Swabbing a car door handle in a public lot to collect DNA is a Fourth Amendment trespass search – Washington Post

DNA Tests, and Sometimes Surprising Results – New York Times

Posted: at 4:36 am


New York Times
DNA Tests, and Sometimes Surprising Results
New York Times
Students at West Chester University in Pennsylvania have volunteered to take part in ancestry DNA testing. Anita Foeman, a communications professor, says she has found that conversations around race are complicated and jagged. CreditWest Chester ...

Read more:
DNA Tests, and Sometimes Surprising Results - New York Times

Posted in DNA | Comments Off on DNA Tests, and Sometimes Surprising Results – New York Times

DNA Ancestry Site Now Tests For Certain Illnesses – CBS Philly

Posted: at 4:36 am

April 23, 2017 9:36 PM By Kristen Johanson

(Photo credit: DANIEL LEAL-OLIVAS/AFP/Getty Images)

PHILADELPHIA (CBS) For about $200 bucks, and a little bit of spit, you can find out your risk of four certain diseases or conditions, but is it something you would want to know?

23andMe, which typically offers ancestry DNA testing, can also look for genetic markers of certain conditions or illnesses, including Parkinsons and late-on-set Alzheimers.

You can do that test, and look through a handful of genes and say to somebody in most cases either we found something that suggests youre at elevated risks, or, we didnt find that thing, said Dr. Steven Joffe, an Associate Professor of Medical Ethics and Health Policy at UPenn.

He says studies show that people who find out they have genes for certain medical conditions dont change behaviors.

I think we have yet to see evidence of lots of benefits from getting this kind of information back, Joffe said.

He says those who get results that they have a genetic variant for a certain condition should see a doctor.

You might be at elevated risk of Alzheimers or Parkinsons. You certainly should be talking to your doctor, or genetic counselor, or somebody who can help you interpret that information put it in context, said Joffe.

The company was approved to test for 10 diseases or illnesses, but right now is only looking at four.

Kristen Johanson is a reporter for KYW Newsradio. She joined the KYW Newsradio news team after spending four years on the assignment desk at our sister television station, CBS-3, as planning editor. She also worked as a field producer for several...

Best US Destinations For Wine Lovers

Videos

About Us

Advertise

Business Development

Contact

Mobile

Connect

CBS Television Public File

CBS Radio Public File

The rest is here:
DNA Ancestry Site Now Tests For Certain Illnesses - CBS Philly

Posted in DNA | Comments Off on DNA Ancestry Site Now Tests For Certain Illnesses – CBS Philly

DNA traits, 300 gauges and other fiction/The Mt. Hope Scrolls – Kingstree News

Posted: at 4:36 am

Hey! Have you seen the commercials about getting your DNA checked? You swab the inside of your mouth and send in a sample and you can find out all kinds of things. You can find out where your ancestors came from and what kind of traits you have. By traits, the report should tell you what kind of food you would eat and whether or not you can run fast. I dont know about you but I cry, Bull to all of that.

One of the ads, claims that a person is from everywhere from India to Western Europe to Oklahoma. That person should run fast, drink coffee and speak Italian. Are you kidding me?

I dont believe any of that is true. Your ancestors may have come from Russia but that doesnt have anything to do with whether or not you would drink vodka. Take a look at any family you know. The environment you live in determines many things. You think? Thats not even true either. Both my parents drank coffee but I never liked the taste of it. That might have been different had my parents made me drink coffee everyday. That would probably not be in my DNA in any way.

Wonder how long these habits or characteristics would take to develop had your ancestors come from a country of fast runners? Just because you have traces of characteristics from several countries in Europe, why dont you speak four languages?

Im not knocking someone wanting to learn about his or her family tree. I just dont think we should over exaggerate what this report will tell us.

Exaggeration is a just part of the advertising process. If exaggeration helps, Go for it.

I recently heard a speaker talking about flying a jet. She proudly proclaimed that her jet had 300 gauges in it. Just imagine going 400 mph and having 300 gauges to look at. Now imagine that it is night.

That sounds really neat but that doesnt mean she can run fast. What it actually means is we all like to exaggerate. In an airplane, there might be a lot of gauges but you dont look at them all at once. The gauges are still there whether you are going 200 or 300. It doesnt matter whether it is day or night. It is just a little exaggeration to help with the talk. Think of all the books in your house. You dont have to read them all at once. Lets just exaggerate about things just a little.

The purpose of all this is to sell more products. Think of all the things you could learn from getting your DNA tested. The jet pilot would be an interesting speaker for your meeting. All of this exaggeration might not be a lie. I certainly dont want to say that. All Im saying is that some ads stretch the validity of a statement. Some exaggerations are just a little too much. Three hundred gauges are probably exaggerated a lot. How can we be sure that the DNA report is correct? You see the point. My favorite exaggeration is doing something a million times. That should show that we have a lot of experience.

Instead it just shows that we exaggerate. A million times does show lots of experience. That is doing something once a day for 2,700 years. Thats a lot no matter where your ancestors are from.

Follow this link:
DNA traits, 300 gauges and other fiction/The Mt. Hope Scrolls - Kingstree News

Posted in DNA | Comments Off on DNA traits, 300 gauges and other fiction/The Mt. Hope Scrolls – Kingstree News

CRISPR genome editing and immunotherapy the early adopter – Medical Xpress

Posted: at 4:36 am

April 24, 2017 Credit: Cancer Research UK

It's been a couple of years since the genome editing tool CRISPR first hit the headlines. And talk of its potential to cure all manner of diseases, create superhumans and bring dinosaurs back from the dead has followed.

But among that speculation, one area of medicine has been quick to pick up the technology and is now leading the way in early clinical trials.

In this second post in our series taking a closer look at CRISPR, we explore its potential for new developments in cancer immunotherapy.

Immunotherapy can take a range of forms. Some experimental approaches use viruses that kill cancer cells and alert the immune system to attack. Others involve giving patients drugs that release the 'brakes' on immune cells to target cancer. And some use specially engineered immune cells that when injected into a patient have the potential to hunt out and kill cancer cells.The aim of immunotherapy treatments is to alert the body's immune system to cancer, so that it's better equipped to recognise and fight the disease.

In each of these cases, scientists need to be able to understand and fine-tune the body's complex immune system. And some are turning to CRISPR for help.

Dr Martin Pule, a clinical senior lecturer in haematology at UCL, says that genome editing techniques such as CRISPR have quickly become part of the tool-kit for researchers like him.

"In the past, many of the technical problems around introducing new genes into cells were worked out, but we didn't have an easy way of efficiently and precisely disrupting existing genes," he says. "New genome editing technologies changed all that."

By using CRISPR, scientists are able to tweak specific genes in viruses or the body's own immune cells, and so make them behave differently.

Researchers have been able to do this before using similar techniques, but the excitement around CRISPR is that this can be done much quicker, cheaper and more precisely than ever before.

Out of the lab, into the patient

Genome editing techniques have been used in people to treat cancer and other diseases before.

There was lots of excitement when news broke in 2015 of a 1 year old girl with acute lymphoblastic leukaemia (ALL) being treated with a similar editing technique known as TALENs, after all other treatments had failed.

She received a transplant of cancer-fighting immune T cells from a donor, which had been tweaked in the lab to give them 2 new characteristics.

Normally, the donated cells would see their new environment as foreign and attack the patient's healthy cells, but genes that control this process were turned off. The T cells would also be susceptible to attack from the anti-cancer drugs that the baby was receiving, and so modifications were made to protect them.

She responded well to the treatment, and another infant received a similar therapy.

Following in the footsteps of its cousin TALENs, CRISPR itself has moved on from the lab to clinical trials. Late last year, a Chinese group became the first to use CRISPR-edited cells in humans.

The team took immune cells from a patient with an aggressive lung cancer and edited them in the lab. This editing deactivates a gene that allows tumours to put the 'brakes' on these immune cells, preventing them from attacking cancer cells.

By switching off the gene, which produces a molecule on the cells' surface called PD-1, the full force of the body's immune system is released, helping it clear the tumour. Drugs that target PD-1 are among the much-lauded immunotherapy treatments already showing promise in advanced melanoma and lung cancers. So there's a lot of hope that CRISPR may provide another step forward here too.

10 patients will be involved in the early-stage Chinese trial, and it will look at whether the treatment is safe, rather than testing effectiveness.

The scientists are also hoping to start clinical trials using CRISPR to treat bladder,prostateandkidney cancers. It's also positive news that both blood cancers and solid tumours appear to be responding to various immunotherapy approaches, as different challenges are faced in treating these diseases.

Kickstart the CAR

One clever immunotherapy trick fuses together 2 components of the immune system with different jobs.

Chimeric antigen receptor (CAR) T cells are a mix of an antibody molecule, which can home in on a specific target on tumour cells, fused to a T cell that provides the knock-out blow to the cancer cell.

We've blogged before about how these engineered cells work, and small trials in 2011 caused lots of excitement. But one of the latest updates is that using CRISPR instead of older genome editing techniques might supercharge these CAR T-cells even further.

The older technology is less precise and can result in the genes mistakenly being inserted at random locations in the cell's DNA. The knock-on effect is that the engineered cells' might be less effective or unintended side-effects could be introduced.

But a US-based group found that CRISPR improved the precision with which the modified gene was inserted into T cells. Their research suggests that the cells were then more potent in their fight against leukaemia in mice because they had more stamina. The researchers are now hoping to test these findings in people.

"Cancer cells are relentless in their attempt to evade treatment, so we need CAR T cells that can match and outlast them," Dr Michel Sadelain, the researcher leading the study at Memorial Sloan Kettering Cancer Center, said at the time.

It's findings like these that will hopefully make engineered immune cell treatments better and kinder in the future, though they aren't yet the Holy Grail.

"In one kind of leukaemia called B-ALL, almost 100% of children who received engineered T cells responded, despite having a disease which had become resistant to all standard treatments," says Pule.

This suggests that, in some circumstances, there may not be an upper limit on who may respond to these treatments. But achieving this in other cancers will take further fine-tuning. In other diseases, such as another kind of blood cancer called DLBCL, the response rates are more like 60%.`

"This reflects the fact that a good CAR T cell product is hard to make, or that there are factors inside the tumour making the T cells less effective," Pule adds.

Lots of the progress using CAR T cells has so far been in blood cancers rather than solid tumours, which have even tougher conditions.

Because CRISPR allows scientists to do lots of small-scale tinkering, this is a rapidly developing field and researchers are trying to find solutions.

"Right now a lot of people are asking why there's this response gap between DLBCL and B-ALL. Can we edit something in the CAR T cell, or put something extra in which will increase the response rates?"

One reason might be that the tumour lives in a hostile environment that stops the engineered T-cells' ability to attack the cancer cells. One way around this is to delete the molecules on T cells that coordinate the stop messages from the microenvironment.

"This strategy looks like it might be quite effective and could increase the number of patients who respond," says Pule.

The other side of immunotherapy

Some of the research that's taken place since CRISPR burst onto the scene has also raised more questions than answers. The immune system is a powerful and complicated machine, and we don't yet understand how to control it.

Not all of these treatments have been as successful as hoped. As well as varying response rates, they can also cause serious side effects, including, in rare cases, death.

There have been recent reports of patients with bladder cancer whose tumours increased in size after immunotherapy treatment, although this has caused some debate among researchers. Side effects including extreme fever or organ damage have also been well documented in clinical trials.

In the US, a total of 5 patients died following treatment with an experimental CAR T cell therapy for ALL. The clinical trial was paused after 3 people died, and then stopped after 2 more deaths.

While this is very rare, it's clear that as well as working to make treatments more effective in more people, researchers also need to look at how they can reduce side effects.

Similar problems were seen in the past in the early days of treatments such as combination chemotherapy, before they were refined.

Pule points to how scientists are already using genome editing to increase safety. For example, in many cases, it isn't possible to engineer a patient's own T cells and so cells from a donor are needed.

But this raises some challenges.

"The donor T cells might attack the recipient causing graft-versus-host disease," says Pule. Graft-versus-host disease is a condition where the donor cells see their new environment as foreign and attack it. "If we remove a specific molecule in the donor T cells using gene-editing technology, we can reduce the chance of this happening."

This is how the two infants with ALL were treated.

Where next?

Like many new technologies, CRISPR was greeted with excited fanfare in some parts, and a more cautious realism is now settling in.

It's clear that CRISPR opens up so many doors for immunotherapy and lets researchers go further, more easily than ever before. But as the technology is understood better, its limitations and challenges also come into focus.

The third part of our CRISPR series will take a look at what the future might hold for CRISPR and cancer research.

Explore further: CAR T cells more powerful when built with CRISPR, researchers find

Cellular therapy hasn't had much success in fighting solid tumors, partly because it's been difficult to deliver anti-cancer T cells to the tumors.

In lung cancer patients who were taking immunotherapy drugs targeting the PD-1 pathway, testing for CD8 T cell activation in their blood partially predicted whether their tumors would shrink. The results are scheduled for ...

Researchers at Houston Methodist demonstrated that a surface protein called OX40, responsible for keeping one type of immune system cell alive, can trigger the death of liver immune cells, in turn starting a chain reaction ...

Artificial intelligencecommonly known as A.I.is already exceeding human abilities. Self-driving cars use A.I. to perform some tasks more safely than people. E-commerce companies use A.I. to tailor product ads to customers' ...

Although the health risks associated with indoor tanning are clear, tanning bed use among college-aged women is still popular. A new study by UNC Lineberger Comprehensive Cancer Center researchers suggests that adding images ...

The activity of cancer drugs changes depending on the types of microbes living in the gut, according to a UCL-led study into how nematode worms and their microbes process drugs and nutrients.

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Here is the original post:
CRISPR genome editing and immunotherapy the early adopter - Medical Xpress

Posted in Genome | Comments Off on CRISPR genome editing and immunotherapy the early adopter – Medical Xpress

Page 1,873«..1020..1,8721,8731,8741,875..1,8801,890..»