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Category Archives: Genetic Engineering

Genetic Engineering Simplified – Video

Posted: May 10, 2014 at 12:48 pm


Genetic Engineering Simplified
Our team made a brief explanation about Genetic Engineering. Don #39;t forget to visit http://www.chemistriology.webs.com for more videos.

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Living organism made with artificial genetic code – Video

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Living organism made with artificial genetic code
Researchers at the Scripps Research Institute say they created the first microbes containing artificial DNA. CBS News medical contributor Dr. David Agus discusses the scientific breakthrough....

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Vermont to require labeling of genetically modified foods

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Nature News Blog

09 May 2014 | 19:32 BST | Posted by Heidi Ledford | Category: Biology & Biotechnology, Politics

Vermont Gov. Peter Shumlin has signed a law mandating the labeling of genetically engineered foods. Picture credit: Community College of Vermont via Flickr

Vermont is the first US state to mandate labels on foods produced using genetic engineering.

Under a law signed by Vermont governor Peter Shumlin on 8 May, labels must be in place on food sold in Vermont by July 2016.

We have a right to know whats in the food we buy, said Shumlin during the signing, as attendees noshed on free Ben & Jerrys ice cream. I am proud that were leading the way in the United States to require labeling of genetically engineered food.

A host of other states are contemplating similar legislation. But even as consumer activists celebrated Vermonts label law, the Grocery Manufacturers Association, a food-industry group based in Washington DC, pledged to file a lawsuit in federal court with the intention of overturning the law. And last month, Congressman Mike Pompeo (Republican, Kansas) introduced the Safe and Accurate Food Labeling Act of 2014in the US House of Representatives, a bill that allows requirements for labeling of genetically engineered food only when that food differs substantially in make-up from non-engineered counterparts. The use of bioengineering does not, in itself, constitute a material difference, the bill states.

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SAY NO TO GENETIC ENGINEERING ! SWINE FLU 2009 – Video

Posted: May 9, 2014 at 12:46 pm


SAY NO TO GENETIC ENGINEERING ! SWINE FLU 2009

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Genetic Engineering Intro – Video

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Genetic Engineering Intro
Video Scribe Project.

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New DNA cleavage technique could lead to more versatile genetic engineering

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3 hours ago Figure 1: Quantitative base-induced DNA cleavage (QBIC) is a technique that allows DNA to be cleaved at any thymine site. Credit: lvcandy/iStock/Thinkstock

Genetic engineering of plants, animals and microorganisms such as bacteria typically involves the use of restriction enzymes to 'cut and paste' DNA fragments into certain genetic sequence locations. This process allows scientists to introduce new genes into an organism, but is constrained to specific recognition sequences, limiting the design of recombinant DNA molecules.

A research team led by Hiroki Ueda and colleagues from the Laboratory for Synthetic Biology at the RIKEN Quantitative Biology Center has now developed a chemical-based, non-enzymatic recombination technique that instead uses a DNA base analogue called 5-ethynyluracil to cleave DNA at any site containing the nucleotide thymine.

The technique developed by Ueda and his co-workers, which is called quantitative base-induced DNA cleavage (QBIC), starts with the generation of DNA fragments containing 5-ethynyluracil in place of thyminetwo molecules with similar structures. These products are then immersed in an aqueous solution containing methylamine, a derivative of ammonia. In this chemical bath, all the nucleotides containing 5-ethynyluracil become cleaved, introducing gaps near the cleaved ends. The gaps in the resulting DNA fragments create protruding ends that can be inserted into circular DNA molecules known as plasmids. The plasmids can then be inserted into the target organism, such as a bacterial cell, to complete the genetic engineering process.

"Compared with restriction enzymes, the QBIC reaction has the advantage that we can freely design the sequences at the protruding termini generated by the DNA cleavage," says Katsuhiko Matsumoto from the research team. "The experimental procedure for DNA concatenation using the QBIC reaction is also simple," he adds. "DNA can be concatenated by the addition and removal of methylamine, hybridized by heating and cooling, and incorporated into an organismin this case the bacterium Escherichia coli."

Another potential boon of the QBIC method is that it is less sensitive to laboratory conditions than enzyme-based techniques and can be run at room temperature. Being a chemical method, it is also generally cheaper to perform than enzyme-based methods. One limitation of the QBIC method in its present form is that long stretches of DNA can lose their structure after treatment with the methylamine solution, which prevents the two-stranded, helical shape from being restored. Ueda's team is now refining the protocol to extend its ability to handle longer DNA fragments. "If we find a solution to this problem," Matsumoto notes, "the QBIC method would become very attractive for the concatenation of long DNA fragments."

Explore further: New method for mass-producing high-quality DNA molecules

More information: Ikeda, S., Tainaka, K., Matsumoto, K., Shinohara, Y., Ode, K. L., Susaki, E. A. & Ueda, H. R. "Non-enzymatic DNA cleavage reaction induced by 5-ethynyluracil in methylamine aqueous solution and application to DNA concatenation." PLoS ONE 9, e92369 (2014). DOI: 10.1371/journal.pone.0092369

Journal reference: PLoS ONE

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Promising role for interleukin-10 in scarless wound healing

Posted: May 8, 2014 at 12:48 pm

PUBLIC RELEASE DATE:

8-May-2014

Contact: Vicki Cohn vcohn@liebertpub.com 914-740-2156 Mary Ann Liebert, Inc./Genetic Engineering News

New Rochelle, NY, May 8, 2014The powerful anti-inflammatory compound interleukin-10 (IL-10) plays a crucial role in regenerative, scarless healing of fetal skin. Studies of IL-10 in postnatal skin wounds have demonstrated its promise as an anti-scarring therapeutic agent, as described in a Critical Review article published in Advances in Wound Care, a monthly peer-reviewed journal from Mary Ann Liebert, Inc., publishers and an Official Journal of the Wound Healing Society. The article is available free on the Advances in Wound Care website.

In "Regenerative Wound Healing: The Role of Interleukin-10," Sundeep Keswani and co-authors, Cincinnati Children's Hospital Medical Center (OH), and Children's Hospital Colorado and the University of Colorado School of Medicine, Aurora, review the complex processes, cell types, growth factors, and other agents needed for successful wound healing. The authors explore the ability of fetal skin to heal without scars and describe the results of ongoing studies to develop IL-10 as an anti-scarring agent.

"Regenerative healing in adults is approachable through lessons learnt from fetal wounds," says Editor-in-Chief Chandan K. Sen, PhD, Professor of Surgery and Director of the Comprehensive Wound Center and the Center for Regenerative Medicine and Cell-Based Therapies at The Ohio State University Wexner Medical Center, Columbus, OH.

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About the Journal

Advances in Wound Care is a monthly peer-reviewed journal published online and in print that reports the latest scientific discoveries, translational research, and clinical developments in acute and chronic wound care. Each issue provides a digest of the latest research findings, innovative wound care strategies, industry product pipeline, and developments in biomaterials and skin and tissue regeneration to optimize patient outcomes. The broad scope of applications covered includes limb salvage, chronic ulcers, burns, trauma, blast injuries, surgical repair, skin bioengineering, dressings, anti-scar strategies, diabetic ulcers, ostomy, bedsores, biofilms, and military wound care. Complete tables of content and a sample issue may be viewed on the Advances in Wound Care website.

About the Publisher

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Vermont governor to sign GMO labeling bill

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Vermont Governor Pat Shumlin intends to sign legislation requiring the labeling of foods that contain ingredients that are genetically modified (GMOs) or produced with genetic engineering. The governors office announced via Twitter that the signing will take place Thursday, May 8. Once the bill is signed, Vermont will become the first state to require mandatory GMO labeling.

The Vermont House approved the bill as amended by the Senate by a vote of 114-30 on April 24. The proposed effective date is July 1, 2016.

It is estimated that 80% of all food sold in the United States is at least partially produced from genetic engineering. The bill would require labeling on all such food sold at retail in Vermont, regardless of whether the food was manufactured in the state. Vermont lawmakers included a $1.5 million legal defense fund in the bill because they expect the law to face legal challenges after the signing.

While the bill exempts processing aids and milk from cows that have been fed GMO feed, many dairy products and other foods that incorporate milk would be affected unless they were made with organic ingredients.

New York GMO bill advances

The New York General Assemblys Committee on Consumer Affairs and Protection advanced a bill on Tuesday that would require the labeling of GMOs. The committee voted 9-6 to approve AB 3525 and now will send the bill to the Committee on Codes. The bill is similar to Vermonts legislation as it would take effect without needing the surrounding states to pass labeling bills.

The New York legislation contains the same exemptions for processing aids and milk from cows that have been fed GMO feed or treated with GMO material. The bill must be approved by both the Assembly and the state Senate before the June 19 recess date and signed by the governor before it can become law.

The Food and Drug Administration, American Medical Association, World Health Organization, USDA and the National Academy of Sciences all have said that GMO ingredients are safe and there are no negative health effects associated with their use.

The International Dairy Foods Association (IDFA) and many other trade organizations oppose individual state legislation on GMO labeling and fully supportThe Safe and Accurate Food Labeling Act of 2014introduced last month by Reps. Mike Pompeo (R-KS) and G.K. Butterfield (D-NC). This bill would preempt states from requiring mandatory labeling and establish a federal standard for voluntary labeling of food and beverage products made with GMOs.

IDFA is working with the Safe and Affordable Food Coalition, headed by the Grocery Manufacturers Association, on issues related to GMO labeling.

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Health screening for low-income women under health care reform: Better or worse?

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PUBLIC RELEASE DATE:

8-May-2014

Contact: Vicki Cohn vcohn@liebertpub.com 914-740-2100 Mary Ann Liebert, Inc./Genetic Engineering News

New Rochelle, NY, May 8, 2014When Massachusetts enacted its own statewide health insurance reform in 2006, low-income women transitioned from receiving free, federally subsidized screening for breast and cervical cancer and cardiovascular disease risk to an insurance-based payment system. The effects on screening rates in this vulnerable population are explored in Journal of Women's Health, a peer-reviewed publication from Mary Ann Liebert, Inc., publishers. The article is available free on the Journal of Women's Health website at http://online.liebertpub.com/doi/full/10.1089/jwh.2013.4612.

A group of authors from Harvard Medical School, Brigham and Women's Hospital, Massachusetts General Hospital, and several women's health centers and community hospitals in Boston, MA gathered data to evaluate whether the prevalence of screening mammography, Pap smear, and blood pressure measurement improved, stayed the same, or declined pre- and post-health insurance reform. In the article "Preventive Care for Low-Income Women in Massachusetts Post-Health Reform," the authors reviewed screening information for women treated at five community health centers between 2004 and 2010, spanning the period before and after the introduction of health reform.

"There are lessons learned from the Massachusetts experience of health care reform that can help inform health care changes nationally," says Susan G. Kornstein, MD, Editor-in-Chief of Journal of Women's Health, Executive Director of the Virginia Commonwealth University Institute for Women's Health, Richmond, VA, and President of the Academy of Women's Health.

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About the Journal

Journal of Women's Health, published monthly, is a core multidisciplinary journal dedicated to the diseases and conditions that hold greater risk for or are more prevalent among women, as well as diseases that present differently in women. The Journal covers the latest advances and clinical applications of new diagnostic procedures and therapeutic protocols for the prevention and management of women's healthcare issues. Complete tables of content and a sample issue may be viewed on the Journal of Women's Health website at http://www.liebertpub.com/jwh. Journal of Women's Health is the official journal of the Academy of Women's Health and the Society for Women's Health Research.

About the Academy

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Scientists Add New Letters to Lifes Genetic Alphabet

Posted: May 7, 2014 at 11:45 pm

Scientists have created the first organism with synthetic DNA that can replicate in a cell, an achievement that promises to add new letters to the genetic code underlying life on earth.

In the natural world, just two chemical base pairs, known simply as A-T and C-G, constitute the building blocks of DNA in all life forms. Research published today in the scientific journal Nature describes the creation of a cell that contains a man-made base pair, dubbed d5SICS-dNAM.

By expanding the natural boundaries of what constitutes life, scientists hope they can one day create new proteins that can handle a variety of chores in the body, potentially leading to unique ways to attack disease. The approach is safe, the researchers said, because it includes a chemical additive that the cell needs to survive.

We created an organism that lives and stably harbors genetic information in its DNA, said Floyd Romesberg, a chemist at La Jolla, California-based Scripps Research Institute, whose laboratory created the new organism. Instead of two base pairs, it has a third.

All life on earth is based on the combination of four chemicals. Adenine bonds naturally with thymine to create the A-T section of the formula, while guanine and cytosine make up the C-G part. The joining of these base pairs in different combinations creates amino acids and proteins that power life.

Romesbergs work differs from other research in the field of genetic engineering in that it involves creation of components that are purely synthetic and integrated into the machinery of life in a cell, he said in a telephone interview.

Other scientists in the field, notably J. Craig Venter, work by constructing genetic material from natural building blocks, or natural components of DNA and proteins.

Starting in 2009, Romesberg and his laboratory created about 300 nucleotides with the newly constituted DNA before landing on ones they believed might be able to replicate in a cell. They then used a special chemical transporter to get the synthetic base pair into an E.coli cell, where it replicated without affecting cell growth. That suggests it wasnt recognized as atypical by the bodys natural DNA repair machinery, according to the paper.

Synthorx Inc., a San Diego-based biotechnology company, has exclusive rights to the synthetic biology from Scripps. The company plans to focus on developing the technology for use in vaccines, medicines and diagnostics, the company said today in a statement.

The research, though promising for fields of medicine and drug development, will probably raise ethical and safety concerns, said Arthur Caplan, head of the division of bioethics at NYU Langone Medical School. He called the research promising.

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