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Category Archives: Genetic Engineering
Photosynthesis hack is needed to feed the world by 2050
Posted: March 27, 2015 at 12:45 pm
Using high-performance computing and genetic engineering to boost the photosynthetic efficiency of plants offers the best hope of increasing crop yields enough to feed a planet expected to have 9.5 billion people on it by 2050, researchers report in the journal Cell.
There has never been a better time to try this, said University of Illinois plant biology professor Stephen P. Long, who wrote the report with colleagues from Illinois and the CAS-MPG Partner Institute of Computational Biology in Shanghai.
"We now know every step in the processes that drive photosynthesis in C3 crop plants such as soybeans and C4 plants such as maize," Long said. "We have unprecedented computational resources that allow us to model every stage of photosynthesis and determine where the bottlenecks are, and advances in genetic engineering will help us augment or circumvent those steps that impede efficiency."
Substantial progress has already been made in the lab and in computer models of photosynthesis, Long said.
"Our lab and others have put a gene from cyanobacteria into crop plants and found that it boosts the photosynthetic rate by 30 percent," he said.
Photosynthetic microbes offer other clues to improving photosynthesis in plants, the researchers report. For example, some bacteria and algae contain pigments that utilize more of the solar spectrum than plant pigments do. If added to plants, those pigments could bolster the plants' access to solar energy.
Some scientists are trying to engineer C4 photosynthesis in C3 plants, but this means altering plant anatomy, changing the expression of many genes and inserting new genes from C4 plants, Long said.
"Another, possibly simpler approach is to add to the C3 chloroplast the system used by blue-green algae," he said. This would increase the activity of Rubisco, an enzyme that catalyzes a vital step of the conversion of atmospheric carbon dioxide into plant biomass. Computer models suggest adding this system would increase photosynthesis as much as 60 percent, Long said.
Computer analyses of the way plant leaves intercept sunlight have revealed other ways to improve photosynthesis. Many plants intercept too much light in their topmost leaves and too little in lower leaves; this probably allows them to outcompete their neighbors, but in a farmer's field such competition is counterproductive, Long said.
Studies headed by U. of I. plant biology professor Donald Ort aim to make plants' upper leaves lighter, allowing more sunlight to penetrate to the light-starved lower leaves. Computer modeling of photosynthesis also shows researchers where the traffic jams occur -- the steps that slow the process down and reduce efficiency.
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Photosynthesis hack is needed to feed the world by 2050
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Report: Photosynthesis hack needed to feed the world by 2050
Posted: at 12:45 pm
IMAGE:This photo shows University of Illinois field trials of various photosynthesis hacks. view more
CHAMPAIGN, Ill. -- Using high-performance computing and genetic engineering to boost the photosynthetic efficiency of plants offers the best hope of increasing crop yields enough to feed a planet expected to have 9.5 billion people on it by 2050, researchers report in the journal Cell.
There has never been a better time to try this, said University of Illinois plant biology professor Stephen P. Long, who wrote the report with colleagues from Illinois and the CAS-MPG Partner Institute of Computational Biology in Shanghai.
"We now know every step in the processes that drive photosynthesis in C3 crop plants such as soybeans and C4 plants such as maize," Long said. "We have unprecedented computational resources that allow us to model every stage of photosynthesis and determine where the bottlenecks are, and advances in genetic engineering will help us augment or circumvent those steps that impede efficiency."
Substantial progress has already been made in the lab and in computer models of photosynthesis, Long said.
"Our lab and others have put a gene from cyanobacteria into crop plants and found that it boosts the photosynthetic rate by 30 percent," he said.
Photosynthetic microbes offer other clues to improving photosynthesis in plants, the researchers report. For example, some bacteria and algae contain pigments that utilize more of the solar spectrum than plant pigments do. If added to plants, those pigments could bolster the plants' access to solar energy.
Some scientists are trying to engineer C4 photosynthesis in C3 plants, but this means altering plant anatomy, changing the expression of many genes and inserting new genes from C4 plants, Long said.
"Another, possibly simpler approach is to add to the C3 chloroplast the system used by blue-green algae," he said. This would increase the activity of Rubisco, an enzyme that catalyzes a vital step of the conversion of atmospheric carbon dioxide into plant biomass. Computer models suggest adding this system would increase photosynthesis as much as 60 percent, Long said.
Computer analyses of the way plant leaves intercept sunlight have revealed other ways to improve photosynthesis. Many plants intercept too much light in their topmost leaves and too little in lower leaves; this probably allows them to outcompete their neighbors, but in a farmer's field such competition is counterproductive, Long said.
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Report: Photosynthesis hack needed to feed the world by 2050
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Biotechnology Part I – Mr Pauller – Video
Posted: March 26, 2015 at 10:48 am
Biotechnology Part I - Mr Pauller
This video presents the topic of biotechnology. Included in the discussion are: genetic engineering, PCR, plasmids, cloning, and restriction enzymes.
By: Noel Pauller
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Biotechnology Part I - Mr Pauller - Video
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Researchers discover genetic origins of myelodysplastic syndrome using stem cells
Posted: at 10:48 am
(New York - March 25, 2015) Induced pluripotent stem cells (iPSCs) -- adult cells reprogrammed back to an embryonic stem cell-like state--may better model the genetic contributions to each patient's particular disease. In a process called cellular reprogramming, researchers at Icahn School of Medicine at Mount Sinai have taken mature blood cells from patients with myelodysplastic syndrome (MDS) and reprogrammed them back into iPSCs to study the genetic origins of this rare blood cancer. The results appear in an upcoming issue of Nature Biotechnology.
In MDS, genetic mutations in the bone marrow stem cell cause the number and quality of blood-forming cells to decline irreversibly, further impairing blood production. Patients with MDS can develop severe anemia and in some cases leukemia also known as AML. But which genetic mutations are the critical ones causing this disease?
In this study, researchers took cells from patients with blood cancer MDS and turned them into stem cells to study the deletions of human chromosome 7 often associated with this disease.
"With this approach, we were able to pinpoint a region on chromosome 7 that is critical and were able to identify candidate genes residing there that may cause this disease," said lead researcher Eirini Papapetrou, MD, PhD, Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai.
Chromosomal deletions are difficult to study with existing tools because they contain a large number of genes, making it hard to pinpoint the critical ones causing cancer. Chromosome 7 deletion is a characteristic cellular abnormality in MDS and is well-recognized for decades as a marker of unfavorable prognosis. However, the role of this deletion in the development of the disease remained unclear going into this study.
Understanding the role of specific chromosomal deletions in cancers requires determining if a deletion has observable consequences as well as identifying which specific genetic elements are critically lost. Researchers used cellular reprogramming and genome engineering to dissect the loss of chromosome 7. The methods used in this study for engineering deletions can enable studies of the consequences of alterations in genes in human cells.
"Genetic engineering of human stem cells has not been used for disease-associated genomic deletions," said Dr. Papapetrou. "This work sheds new light on how blood cancer develops and also provides a new approach that can be used to study chromosomal deletions associated with a variety of human cancers, neurological and developmental diseases."
Reprogramming MDS cells could provide a powerful tool to dissect the architecture and evolution of this disease and to link the genetic make-up of MDS cells to characteristics and traits of these cells. Further dissecting the MDS stem cells at the molecular level could provide insights into the origins and development of MDS and other blood cancers. Moreover, this work could provide a platform to test and discover new treatments for these diseases.
###
This study was supported by grants from the National Institutes of Health, the American Society of Hematology, the Sidney Kimmel Foundation for Cancer Research, the Aplastic Anemia & MDS International Foundation, the Ellison Medical Foundation, the Damon Runyon Cancer Research Foundation, the University of Washington Royalty Research Fund, and a John H. Tietze Stem Cell Scientist Award.
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Snapchat or Facebook — which one is more likely to elicit romantic jealousy?
Posted: at 10:48 am
IMAGE:Cyberpsychology, Behavior, and Social Networking is an authoritative peer-reviewed journal published monthly online with Open Access options and in print that explores the psychological and social issues surrounding... view more
Credit: Mary Ann Liebert, Inc., publishers
New Rochelle, NY, March 25, 2015--The photo-sharing app Snapchat is not yet as popular as Facebook for social networking, but the greater privacy Snapchat may offer could motivate users to share more intimate types of content for different purposes. A new study comparing Snapchat and Facebook use and their effect on romantic relationships is published in Cyberpsychology, Behavior, and Social Networking, a peer-reviewed journal from Mary Ann Liebert, Inc., publishers. The article is available free on the Cyberpsychology, Behavior, and Social Networking website until April 25, 2015.
The article "Snapchat Elicits More Jealousy Than Facebook: A Comparison of Snapchat and Facebook Use" describes a study comparing how individuals use the two social networking apps, and whether Snapchat, with which messages disappear after only a few seconds and are typically sent to a smaller number of people, affords more private communication and intimate, personal content that could evoke greater jealousy. Authors Sonja Utz and Nicole Muscanell, Knowledge Media Research Center (Tbingen, Germany), and Cameran Khalid (Glasgow University, Scotland), found that behaviors of romantic partners on Snapchat evoked higher levels of jealousy than did the same behaviors on Facebook.
"Although a small preliminary study, this is an important foray into a new communication platform," says Editor-in-Chief Brenda K. Wiederhold, PhD, MBA, BCB, BCN, Interactive Media Institute, San Diego, California and Virtual Reality Medical Institute, Brussels, Belgium. "And with the January 2015 Snapchat update, which made Best Friends Lists private, one wonders if we will now see the fire of jealousy further inflamed."
###
About the Journal
Cyberpsychology, Behavior, and Social Networking is an authoritative peer-reviewed journal published monthly online with Open Access options and in print that explores the psychological and social issues surrounding the Internet and interactive technologies, plus cybertherapy and rehabilitation. Complete tables of content and a sample issue may be viewed on the Cyberpsychology, Behavior, and Social Networking website.
About the Publisher
Mary Ann Liebert, Inc., publishers is a privately held, fully integrated media company known for establishing authoritative peer-reviewed journals in many promising areas of science and biomedical research, including Games for Health Journal, Telemedicine and e-Health, and Journal of Child and Adolescent Psychopharmacology. Its biotechnology trade magazine, Genetic Engineering & Biotechnology News (GEN), was the first in its field and is today the industry's most widely read publication worldwide. A complete list of the firm's 80 journals, books, and newsmagazines is available on the Mary Ann Liebert, Inc., publishers website.
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Snapchat or Facebook -- which one is more likely to elicit romantic jealousy?
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Drugs companies unite to mine genetic data
Posted: at 10:48 am
Nonetheless, he said, drug companies would only be allowed access to the data in a tightly controlled way, describing the project as "more like a reading library than a lending library".
Martin Mackay, head of research and development at Alexion, a US biotech company taking part in the consortium, said the scheme had the potential to "accelerate the understanding of the genetic basis of rare diseases and ultimately lead to improved diagnostics and treatments".
The other members of the alliance called the Gene Consortium are UCB of Belgium, Takeda of Japan, and Dimension Therapeutics and Helomics of the US.
Genomics England will also announce partnerships with scientists and academic institutions in another move to make its database available to researchers.
The 100,000 Genomes Project is part of efforts by the government to use the NHS and its wealth of medical data to attract international medical research and life sciences investment.
Sir John is a veteran of the software and engineering industries who oversaw the privatisation of Qinetiq, the government's defence technology service business.
"I spent a large amount of my working life in the microelectronic revolution which [changed] the 20th century," he told the Financial Times. "Genetics [is] going to change the world in the 21st century."
Genomics England could play an important role, he added, in the shift towards more personalised medicines to tackle the genetic defects of individual patients.
"We will look back on how we used to pour pills down our necks as being not much different to how we look back on bleeding someone who has a fever."
Another large genomic database has been built by 23andMe, a Google-backed Californian company that sells $99 DNA testing kits.
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Mount Sinai Researchers Discover Genetic Origins of Myelodysplastic Syndrome Using Stem Cells
Posted: March 25, 2015 at 2:45 pm
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Available for logged-in reporters only
Newswise (New York March 25, 2015) Induced pluripotent stem cells (iPSCs)adult cells reprogrammed back to an embryonic stem cell-like statemay better model the genetic contributions to each patient's particular disease. In a process called cellular reprogramming, researchers at Icahn School of Medicine at Mount Sinai have taken mature blood cells from patients with myelodysplastic syndrome (MDS) and reprogrammed them back into iPSCs to study the genetic origins of this rare blood cancer. The results appear in an upcoming issue of Nature Biotechnology.
In MDS, genetic mutations in the bone marrow stem cell cause the number and quality of blood-forming cells to decline irreversibly, further impairing blood production. Patients with MDS can develop severe anemia and in some cases leukemia also known as AML. But which genetic mutations are the critical ones causing this disease?
In this study, researchers took cells from patients with blood cancer MDS and turned them into stem cells to study the deletions of human chromosome 7 often associated with this disease.
With this approach, we were able to pinpoint a region on chromosome 7 that is critical and were able to identify candidate genes residing there that may cause this disease, said lead researcher Eirini Papapetrou, MD, PhD, Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai.
Chromosomal deletions are difficult to study with existing tools because they contain a large number of genes, making it hard to pinpoint the critical ones causing cancer. Chromosome 7 deletion is a characteristic cellular abnormality in MDS and is well-recognized for decades as a marker of unfavorable prognosis. However, the role of this deletion in the development of the disease remained unclear going into this study.
Understanding the role of specific chromosomal deletions in cancers requires determining if a deletion has observable consequences as well as identifying which specific genetic elements are critically lost. Researchers used cellular reprogramming and genome engineering to dissect the loss of chromosome 7. The methods used in this study for engineering deletions can enable studies of the consequences of alterations in genes in human cells.
Genetic engineering of human stem cells has not been used for disease-associated genomic deletions, said Dr. Papapetrou. This work sheds new light on how blood cancer develops and also provides a new approach that can be used to study chromosomal deletions associated with a variety of human cancers, neurological and developmental diseases.
Reprogramming MDS cells could provide a powerful tool to dissect the architecture and evolution of this disease and to link the genetic make-up of MDS cells to characteristics and traits of these cells. Further dissecting the MDS stem cells at the molecular level could provide insights into the origins and development of MDS and other blood cancers. Moreover, this work could provide a platform to test and discover new treatments for these diseases.
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Mount Sinai Researchers Discover Genetic Origins of Myelodysplastic Syndrome Using Stem Cells
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Tom Horn End Time Sings Mixing Human DNA With Animal DNA Audio only – Video
Posted: March 24, 2015 at 5:45 am
Tom Horn End Time Sings Mixing Human DNA With Animal DNA Audio only
Audio Only 2015 August Breaking News Mixing Human DNA with Animal DNA - Last Days End Times News Prophecy Update - Genetic Engineering.
By: Icelandic Watchman
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Tom Horn End Time Sings Mixing Human DNA With Animal DNA Audio only - Video
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'Ban DNA Editing Of Sperm And Eggs'
Posted: at 5:45 am
Designer babies are on their way, said experts in genetic engineering as they called for a global ban on the practice.
It is thought that studies involving the use of genome-editing tools to modify the DNA of human embryos will be published shortly, said the authors of a paper in Nature.
The articles lead author, Professor Jennifer Doudna of the University of California at Berkeley, led the team that developed the gene-editing technique that she now wants restricted.
She and her colleagues have now warned of the ethical and safety implications of research that could lead to the birth of what laymen might term super humans.
In our view, genome editing in human embryos using current technologies could have unpredictable effects on future generations, they said. This makes it dangerous and ethically unacceptable. Such research could be exploited for non-therapeutic modifications.
DNA can be edited far more precisely than ever before using Crispr-Cas9 (Credit: Mehmet Pinarci/Sendercorp)
It is possible, for example, for the technology to make unintended changes to DNA, The New York Times reported.
But they are also worried that a public backlash could halt work on disease fighting techniques in somatic (non-reproductive) cells.
Genome-editing technologies may offer a powerful approach to treat many human diseases, including HIV/Aids, haemophilia, sickle-cell anaemia and several forms of cancer, they said.
Scientists at the Hubrecht Institute in the Netherlands reported in Cell Stem Cell two years ago that the technique could repair the cystic fibrosis mutation.
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'Ban DNA Editing Of Sperm And Eggs'
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Scientists call for caution in using DNA-editing technology
Posted: at 5:45 am
19 hours ago by Robert Sanders The bacterial enzyme Cas9 is the engine of RNA-programmed genome engineering in human cells. Credit: Jennifer Doudna/UC Berkeley
A group of 18 scientists and ethicists today warned that a revolutionary new tool to cut and splice DNA should be used cautiously when attempting to fix human genetic disease, and strongly discouraged any attempts at making changes to the human genome that could be passed on to offspring.
Among the authors of this warning is Jennifer Doudna, the co-inventor of the technology, called CRISPR-Cas9, which is driving a new interest in gene therapy, or "genome engineering." She and colleagues co-authored a perspective piece that appears in the March 20 issue of Science, based on discussions at a meeting that took place in Napa on Jan. 24. The same issue of Science features a collection of recent research papers, commentary and news articles on CRISPR and its implications.
"Given the speed with which the genome engineering field is evolving, our group concluded that there is an urgent need for open discussion of the merits and risks of human genome modification by a broad cohort of scientists, clinicians, social scientists, the general public and relevant public entities and interest groups," the authors wrote.
Doudna, director of UC Berkeley's Innovative Genomics Initiative, was joined by five current and two former UC Berkeley scientists, plus David Baltimore, a Nobel laureate and president emeritus of the California Institute of Technology, Stanford Nobelist Paul Berg and eminent scientists from UC San Francisco, Stanford, Harvard and the universities of Wisconsin and Utah. Several of these scientists are currently involved in gene therapy to cure inherited diseases.
Latest of many warnings
Such warnings have been issued numerous times since the dawn of genetic engineering in 1975, but until now the technology to actually fix genetic defects was hard to use.
"However, this limitation has been upended recently by the rapid development and widespread adoption of a simple, inexpensive and remarkably effective genome engineering method known as CRISPR-Cas9," the scientists wrote. "The simplicity of the CRISPR-Cas9 system enables any researcher with knowledge of molecular biology to modify genomes, making feasible many experiments that were previously difficult or impossible to conduct."
Correcting genetic defects
Scientists today are changing DNA sequences to correct genetic defects in animals as well as cultured tissues generated from stem cells, strategies that could eventually be used to treat human disease. The technology can also be used to engineer animals with genetic diseases mimicking human disease, which could lead to new insights into previously enigmatic disorders.
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Scientists call for caution in using DNA-editing technology
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