Category Archives: DNA

WASHINGTON, Jan. 2, 2013 /PRNewswire/ --New research appearing online today in Clinical Chemistry, the journal of AACC, shows that the analysis of DNA mutations in patients with cancer can be done without surgery. This paper by Chan et al. shows that the plasma of cancer patients also carries tumor-derived DNA. Because the DNA sequencing of plasma is non-invasive, this could lead to cheap and highly informative clinical tools for early cancer diagnosis and personalized therapy selection, granting better health outcomes to all cancer patients.

(Logo: http://photos.prnewswire.com/prnh/20121206/PH25333LOGO )

Because all cancers occur due to abnormalities in DNA, scientists are able to use DNA sequencing to analyze the mutations in each patient's DNA. Once this sequencing is complete, doctors can match each patient to the best available drug for his or her particular cancer, thus personalizing the treatment for each patient and improving health outcomes. In the past, DNA sequencing has required tumor tissue, which could only be obtained by biopsying the tissue an invasive procedure not ideal for the patient. In this study, Chan et al. explored the use of shotgun massively parallel sequencing of plasma DNA from cancer patients to scan a cancer genome without surgery.

The researchers extracted DNA from the tumor tissues of 4 liver patients and 1 breast and ovarian cancer patient, and then analyzed the preoperative and postoperative plasma samples of these patients. Through the use of multiregional sequencing of tumor tissues and shotgun sequencing of plasma DNA, the researchers have shown that plasma DNA sequencing is a powerful tool for cancer detection, monitoring, and research.

"This ground-breaking study uses brand new technology--the multiregional sequencing research tool will lead to routine practice leading to lower cost," said Eleftherios P. Diamandis, MD, PhD, FRCP(C), FRSC, Head of Clinical Biochemistry at Mount Sinai Hospital and Editor of this special issue of Clinical Chemistry. "This is the first time analysis has been done non-invasively instead of performing a biopsy on human tissue. The proof of principle is demonstrated and will be more readily available and cost effective in the future."

About AACC

The American Association for Clinical Chemistry, AACC, is a leading international medical society dedicated to improving healthcare through laboratory medicine. With more than 9,000 clinical laboratory professionals, physicians, research scientists, and other members involved in developing tests and directing laboratory operations, AACC brings the laboratory community together with programs that advance knowledge, expertise, and innovation.

Clinical Chemistry is the leading international journal of clinical laboratory science, providing 2,000 pages per year of peer-reviewed papers that advance the science of the field. With an impact factor of 7.9, Clinical Chemistry covers everything from molecular diagnostics to laboratory management.

Molly Polen AACC Director of Communications & PR (p) 202.420.7612 (c) 703.598.0472 mpolen@aacc.org

Follow this link:
Breakthrough Research in DNA Mutation Analysis Could Lead to Earlier Diagnosis and More Tools for Personalized ...

Improved technology and an expanding DNA database have helped solve thousands of crimes in Texas since the national system was created more than 15 years ago, experts say.

At least 643 homicides are among the Texas cases solved with assistance from the Combined DNA Index System, or CODIS, the San Antonio Express-Newsreported Wednesday.

Many of these crimes might never have been solved if not for this database, said Steve McCraw, director of the Texas Department of Public Safety.

Registered sex offenders, convicted felons and youths in the Texas juvenile justice system must provide samples. The number of samples of offenders DNA stored in the Texas database has mushroomed to more than 660,000.

The more samples in the pool, the greater opportunity for a match, said Skylor Hearn, who oversees the crime lab that manages the states database. There is a degree of recidivism in the criminal world, and were catching up to them.

Texas averaged about 200 matches annually during the first five years after the national database was created in 1996. That number rose to an average 1,000 hits annually for the next 10 years, records show.

The Harris County Institute of Forensic Sciences in Houston uses computer automation to help process large batches of samples.

We used to process just a dozen samples a month, but now were averaging 400. Few other labs in the country can beat that, said the labs director, Dr. Roger Kahn, explaining how automation has replaced the repetitive tasks once done manually.

Harris County also has a special team that it can dispatch to collect potential DNA from sensitive homicide scenes. As a result of these advancements, Kahn said his lab was responsible for nearly 25 percent of the estimated more than 10,000 crimes solved.

Excerpt from:
DNA database helps solve thousands of Texas crimes

Public release date: 3-Jan-2013 [ | E-mail | Share ]

Contact: Beth Parada bparada@botany.org American Journal of Botany

The polymerase chain reaction (PCR) is a common technique used to amplify, or copy, pieces of DNA. Amplified DNA is then used in genetic analyses for everything from medicine to forensics. In plant research, PCR is a vital step in detecting and sequencing genes, and its applications are endless. However, compounds found in plants often inhibit PCR. Researchers at the University of Southern Mississippi discovered that the use of an additive allows PCR to successfully amplify DNA from once problematic plants.

PCR is widely used in plant sciences but is not 100 percent reliable. Many plant researchers encounter roadblocks when implementing PCR. For example, many plant species contain phenolic compounds that deter herbivores. These compounds are often extracted along with plant DNA and can stop PCR from working.

Graduate student Tharangamala Samarakoon and colleagues have found a technique to overcome many of these inhibitory plant compounds. They added a reagent to the PCR mixture that contains three ingredients: trehalose, bovine serum albumin, and polysorbate-20 (all three abbreviated TBT-PAR). "Unlike several other studies, TBT-PAR works at the PCR stage instead of at the DNA extraction stage, so it has promise for pigeon-holed and half-forgotten extractions that previously failed to be amplified using PCR," says Samarakoon. The authors published their research in the January issue of Applications in Plant Sciences.

Samarakoon tested the TBT-PAR reagent on DNA extracted from tropical and temperate species across four plant families, including Achariaceae, Asteraceae, Lacistemataceae, and Samydaceae. PCR with TBT-PAR successfully amplified DNA for all species, whereas standard DNA extraction and PCR techniques consistently failed.

TBT-PAR enhanced PCR for DNA extracted from fresh, silica-dried, and herbarium plant material. "Since we study tropical plants, many of which are geographically restricted or rare," explains Samarakoon, "herbarium material is sometimes all that we have available for DNA extraction, and curators are gracious to allow even a small destructive sampling for a single extraction attempt. We want that one attempt, of course, to be successful." Samarakoon predicts that inhibitory plant compounds could be the underlying cause of many PCR failures in herbarium specimens and hopes TBT-PAR will have widespread benefits in herbarium specimen DNA amplification.

TBT-PAR was first used in the PCR detection of a shrimp virus by co-author Shiao Wang and his colleagues. "The additive has also been helpful in a colleague's lab where they had trouble amplifying DNA from gopher tortoise ticks, so its utility extends beyond plants," comments Samarakoon. TBT-PAR has the potential for broad use in PCR techniques across DNA samples, species, and taxa.

The article will be published in the first issue of Applications in Plant Sciences (APPS), a new journal released by the Botanical Society of America. Theresa Culley, Editor-in-Chief of APPS, describes the new journal as a venue to "expedite the dissemination of innovative information encompassing all areas of the plant sciences, including but not limited to genetics, structure, development, evolution, systematics, and ecology." APPS publishes new methods in plant sciencesan important niche to fill in an age of rapid technological advances.

###

See the original post here:
Improving DNA amplification from problematic plants

HTC Droid DNA -vs- Apple iPhone 5 - HANDS-ON Review For New Year 2013
HTC Droid DNA -vs- Apple iPhone 5 - HANDS-ON Review For New Year 2013

By: Fahad Ahmed

Read more from the original source:
HTC Droid DNA -vs- Apple iPhone 5 - HANDS-ON Review For New Year 2013 - Video

ARPA DNA PROJECT

By: Hagop Panossian

Visit link:
ARPA DNA PROJECT - Video

Ramesses III Y-DNA E1b1a Armana mummies 18th dynasty original version {shorter version}
Video is technically not the original I just made it shorter than the Director cut (original) which explains me having a Director #39;s cut. I cut out a lot of Dr. Keita clips and some quotes to make it less redundant. However, I recommend those who have no problem with the time limit to check out the Director #39;s cut version which flows more with information. video is based off the recent publications of Zahi Hawass 2012.According to a genetic study in December 2012, Ramesses III belonged to Y-DNA haplogroup E1b1a, mainly found in sub-saharan Africa.[26] Hawass at al. 2012, Revisiting the harem conspiracy and death of Ramesses III: anthropological, forensic, radiological, and genetic study Zahi Hawass, egyptologist1, Somaia Ismail, professor of molecular biology23, Ashraf Selim, professor of radiology4, Sahar N Saleem, professor of radiology4, Dina Fathalla, molecular biologist3, Sally Wasef, molecular biologist5, Ahmed Z Gad, molecular biologist3, Rama Saad, molecular biologist3, Suzan Fares, molecular biologist3, Hany Amer, assistant professor of pharmacology6, Paul Gostner, radiologist7, Yehia Z Gad, professor of molecular genetics2, Carsten M Pusch, molecular biologist8, Albert R Zink, paleopathologist9 E1b1a commonly found in 58-60% of African American males and over 90% males in West Africa and peaks as high as 80% in selective regions of East, South, Central Africa. E1b1a is associated with the Bantu migration but is much older, as explained by Dr. Keita. Work is ...

By: davidcici11Evolution

Excerpt from:
Ramesses III Y-DNA E1b1a Armana mummies 18th dynasty original version {shorter version} - Video

Happy New Year From DNA Studios
Happy New Year Hope you like the collage

By: kingoftheworldCM

Go here to see the original:
Happy New Year From DNA Studios - Video

{DNA} [Eddie Redmayne]
Happy new years?? I suppose?? Sparkle credit to ifinishit This isn #39;t my best video, for some reasons I dunno why this video just wasn #39;t flowing. I suppose I want to do more effects but I honestly don #39;t like or know how to do a few. Its just my style of editing I suppose.

By: Jayasolo3

Read more from the original source:
{DNA} [Eddie Redmayne] - Video

Phone Comparison - Samsung Straosphere Vs. HTC Droid DNA
This video covers a comparison between a basic android smartphone vs a higher end android smartphone. As well as my personal opinions / thoughts on these phones.

By: CGComputerInc

See more here:
Phone Comparison - Samsung Straosphere Vs. HTC Droid DNA - Video

Posted: Dec. 31, 2012 | 11:41 a.m. Updated: Dec. 31, 2012 | 12:26 p.m.

The blood of 10-year-old Jade Morris is a positive DNA match to blood on a jumpsuit worn by Brenda Jean Stokes, the 50-year-old expected to be officially charged this week in the child's homicide, according to police.

A Las Vegas police report released Monday paints a clearer picture of the events that lead to Jade's death on Dec. 21. She died from multiple stab wounds. Her body was found Thursday in an unfinished housing development in North Las Vegas.

Police say Stokes used a red 2007 Saab, borrowed from her friend Eddie Turner, to kidnap and transport the girl.

Turner told investigators that on Dec. 21, the day Jade disappeared, Stokes returned the car, but borrowed it again to go to her apartment on Windmill Lane to change her clothes.

Later on that evening, Turner told investigators she dropped off Stokes at The Cosmopolitan on Las Vegas Boulevard, where Stokes said she planned to meet a friend, according to the report.

Instead, Stokes went to the Bellagio, and used a pair of razor blades to slice the face of Joyce Rhone, 44, a blackjack dealer.

Jade's family believes Rhone and Stokes' fiance, Jade's father Philip Morris, were having a fling that lead to the attack. Police have neither confirmed or denied that allegation.

After Stokes' arrest, she told responding officers she regretted not having killed Rhone, who suffered serious cuts to her face, enough to cause "disfigurement," according to the report.

While held on assault and battery charges in the Clark County Detention Center, investigators questioned Stokes on the girl's disappearance. She denied knowing Jade or her family.

Continue reading here:
Dna Match Links Homicide Suspect To 10-Year-Old Girl