Category Archives: DNA

DNA Fingerprint Sequence
Download these videos here: http://goo.gl/hxlgIS This is two different 1080p motion graphic videos from results of a DNA fingerprint sequence that displays the DNA genetic chromosome makeup...

By: Matthew Butler

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DNA Fingerprint Sequence - Video

38 Kill Streak *DNA* *Bomb* On Horizon
Twitch.Tv/JGluff16 Twtter.com/JGluff16.

By: Jared Gluff

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38 Kill Streak *DNA* *Bomb* On Horizon - Video

COD: AW - 25 "Double DNA Bombs" of Christmas! Day 8 - CLUTCH!
Hope you all enjoy the video, let me know any suggestions you have for me! Can we hit 60 likes for another commentary? Follow me - http://twitter.com/clueshh.

By: Clues

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COD: AW - 25 "Double DNA Bombs" of Christmas! Day 8 - CLUTCH! - Video

14 Casiopea Sync DNA - Cercular Dream

By: Randy Wihandika

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14 Casiopea & Sync DNA - Cercular Dream - Video

DNA passes through a pore, the basis of nanopore sequencing.

Nearly three years ago, a company called Oxford Nanopore made waves when it announced thedevelopment of DNA sequencing technology that was simple and compact enough to fit ona USB stick. The intervening years have been filled with product delays and apparent problems with read accuracy. This morning, however, a paper is being released that relies on the technology, and while the technology still has problems, it can help provide some medically relevant information.

Most methods of studying DNA perform what's called "sequencing by synthesis." They make a new copy of the DNA strand being sequenced, keeping track of which base is added to each location. This is highly effective, but it requires the use of consumables: each base added has to be supplied to the reaction, as does the enzyme that does the adding. All of this adds to the cost of the sequencing reaction, and it places a limit on how cheap we can make the process of sequencing a genome.

Oxford Nanopore's method is fundamentally different. Instead of adding new bases, the DNA strand to be sequenced is stuffed through a protein with a hole in the middle. As each base passes through this pore, its electrical properties are read, allowing the hardware to determine which base is going by. Although the pore will have a finite lifetime, the process doesn't need any specialized chemicals or enzymes and therefore has the potential to be very cheap.

It also has the potential to read the sequence of any length of DNA molecule. The most popular DNA sequencing technology, made by Illumina, is not commonlyused toread molecules that are much longer than 100 bases long, creating what's called "short read" sequences. It makes up for the short length by reading incredibly high volumes of DNA molecules.

But high volume doesn't entirely make up for things. In many areas of genomes, there are repeated sequences that are longer than 100 bases longthings like old, disabled viruses and transposable elements that hop around the genome. If a bit of sequence ends up in one of these repetitive elements, then there's no possible way to figure out where it resides in the genome. As a result, these repetitive sequences break up any genomes constructed solely from short reads, limiting our picture of the genome.

IfOxford Nanopore could get its system up and working, there would definitely be a use for it. But three years into things, and the new paper is still describing pre-release hardware. Gone is the claim of it being an actual USB device; instead, it's now referred to as "similar in size to a USB memory stick." But the biggest problem is the error rate; the system gets nearly a third of the bases it sequences wrong.

That may sound pretty useless, but it's not as bad as it seems. If both strands of the DNA molecule happen to be read, then accuracy gets bumped up to over 80 percent. And when combined with short-read Illumina data, it's possible to use it to help build more complete genomes.

The basic idea is that the long reads of the nanopore system can provide a scaffold for the entire genomeone that's error-filled but puts everything in the right order. The high-accuracy short reads from traditional sequencing methods can then fill in the scaffold with accurate data. The authors test this by sequencing a multidrug-resistant Salmonella strain. The drug resistance genes, in this case, were surrounded by repetitive DNA, leaving it unclear whether they were in the bacteria's genome at all, much less where they might be located.

In general, the approach of using both methods worked well. The number of gaps and unordered sequences went down, and a cluster of antibiotic resistance genes was identified in the regular chromosome of this strain. It wasn't entirely without problems, though. It turns out Illumina machines have problems when there are lots of G's and C's in a sequence, so there were a few areas that continued to be low quality. But the end result was a clearer picture of a medically relevant bacterial strain.

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USB-sized DNA sequencer is error-prone, but still useful

A new technique to analyse DNA found in millions of ancient parchments (material made from animal skin) stored in archives can help scientists trace agricultural development across the centuries, according to researchers.

Thanks to increasingly progressive genetic sequencing techniques, the all-important historical tales these documents tell are no longer confined to their texts.

The vital information also comes from the DNA of the parchment on which they are written, say researchers from Trinity College Dublin and the University of York.

"Parchments are an amazing resource for genetic studies that consider agricultural development over the centuries. There must be millions stored away in libraries, archives, solicitors' offices and even in our own attics," said Daniel Bradley, a professor of population genetics at Trinity College Dublin.

Parchment was the writing material of choice for thousands of years, going back to the Dead Sea scrolls.

For the study, the team used state-of-the-art scientific techniques to extract ancient DNA and protein from tiny samples of parchment from documents from the late 17th and late 18th centuries.

The resulting information enabled them to establish the type of animals from which the parchment was made.

Geneticists at Trinity extracted DNA from two tiny samples of parchment.

Meanwhile, researchers at University of York extracted collagen (protein) from the same parchment samples.

The first sample showed a strong affinity with northern Britain, specifically the region in which black-faced breeds such as Swaledale, Rough Fell and Scottish Blackface are common.

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Ancient DNA on parchments can reveal agricultural development

bigiu dna sdb
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By: Stiri de Buzau

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bigiu dna sdb - Video

Call of Duty Advanced Warfare 68-8 DNA Gameplay fail
Call of Duty Advanced Warfare DNA Gameplay fail.

By: BLx Tiiagooh

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Call of Duty Advanced Warfare 68-8 DNA Gameplay fail - Video

Issue Of DNA Test Happened 8 Years Ago -- Femi Kuti
Afrobeat Maestro, Femi Kuti, has spoken out on the much publicised paternity test that proved that his two children were not actually his. the publication had gone viral last week, leaving...

By: Channels Television

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Issue Of DNA Test Happened 8 Years Ago -- Femi Kuti - Video

Advanced Warfare DNA Fail + fous rire : Conseil de grand frre #8 Insomnie
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Advanced Warfare DNA Fail + fous rire : Conseil de grand frre #8 Insomnie - Video