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Category Archives: DNA
DNA 'cage' could improve nanopore technology
Posted: February 11, 2015 at 3:45 pm
Despite having a diameter tens of thousands of times smaller than a human hair, nanopores could be the next big thing in DNA sequencing. By zipping DNA molecules through these tiny holes, scientists hope to one day read off genetic sequences in the blink of an eye.
Now, researchers from Brown University have taken the potential of nanopore technology one step further. They have combined a nanopore with a tiny cage capable of trapping and holding a single DNA strand after it has been pulled through the pore. While caged, biochemical experiments can be performed on the strand, which can then be zipped back through the nanopore to look at how the strand has changed.
"We see this as a very interesting enabling technique," said Derek Stein, associate professor of physics and engineering at Brown, who helped develop the technology with his graduate students. "It allows you for the first time to look at the same molecule before and after any kind of chemical reaction that may have taken place."
A paper describing the device is published in Nature Communications.
The device looks a bit like a miniscule hollowed-out hockey puck. On one flat side is a nanopore, and on the other side is a somewhat larger hole. When immersed in a solution containing DNA, an electric current across the nanopore grabs a single strand and pulls it into the hollow chamber. Once there, the strand has a natural tendency to curl into a tangled ball. That ball is too large to fit out of the hole on the other side, but that hole can be used to introduce additional molecules that might react with the trapped DNA. Once a reaction has occurred, the electric current is reversed and the strand is sent back out through the pore, which can look for changes in the strand.
"What we've made is basically a very small test tube," said Xu Liu, who led the work while he was a graduate student at Brown. "We can do biochemistry on the single strand in that very confined space."
The key to the technology, Liu said, was making that test tube small, but not too small. If it were too small, the DNA wouldn't have enough room to curl up, which would cause it to squirt out the hole at the top of the device. Using some theoretical calculations and a bit of trial and error, the researchers settled on a cage that's about 1.5 micrometers square.
Liu then tested the technology using what's called a restriction enzyme, which cuts DNA molecules at particular sequences. After an intact DNA molecule was pulled through the pore into the cage, the researchers applied the enzyme through the hole in the top of the device. If all went as planned, the enzyme should have cut the strand into four pieces. When they pulled the molecule back through pore, they detected four distinct signals, indicating that the experiment had worked as expected.
The researchers say the device could be used for all kinds of experiments with DNA. For example, scientists use molecules called hybridization probes to look for specific sequences in a DNA molecule. The probes bind to target sequences, creating a bulge in the DNA strand that a nanopore could easily detect.
"There was always a problem of knowing what the DNA looked like before the probe was applied," Stein said. "This is a way of making sure you can measure the same molecule before anything is done to it, and then after. That wasn't possible before with nanopores because the molecule would drift away."
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DNA 'cage' could improve nanopore technology
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'Male Y' DNA in Mohd Saiful's rectum Anwar's, rules Federal Court
Posted: at 3:45 pm
PUTRAJAYA, Feb 10 (Bernama) -- The DNA profile of a "Male Y" found in complainant Mohd Saiful Bukhari Azlan's rectum belonged to Opposition Leader Datuk Seri Anwar Ibrahim, the Federal Court ruled today.
Chief Justice Tun Arifin Zakaria said the evidence of two chemists, Dr Seah Lay Hong and Nor Aidora Saedon, that the DNA profile of Male Y developed from the seminal extract from Mohd Saiful's rectum matched the profile developed from a tooth brush, a Good Morning towel and a mineral water bottle collected from the lock-up occupied by Anwar.
The Federal Court had dismissed Anwar's appeal against a Court of Appeal decision that overturned a High Court judgement and convicted him and sentenced him to five years jail for having sodomised his former aide Mohd Saiful on June 26, 2008.
Justice Arifin said that considering the other evidence, there was no dispute that the samples which Nor Aidora had examined had come from the items that were used solely by Anwar.
"It is thus indisputable that the profile of Male Y developed and analysed by both the chemists belongs to none other than the appellant," he said.
Justice Arifin pointed out that it was indeed a very high probability based on the DNA of the Malaysian population database that the Male Y DNA belongs to the same person.
Hence, the court held that the DNA evidence of Male Y was the corroborative evidence to the element of penile penetration by Anwar.
The court also held that the evidence by the two chemists fell under expert opinion and the panel had no doubt that they were experts and that Anwar had failed to discredit the duo.
"There was nothing inherently incredible about the evidence of PW5 (prosecution witness five Dr Seah) and PW6 (Nor Aidora)," he said.
The panel also found that the recovery of the exhibits used by Anwar while he was in the lock-up was not transgression of any rule, nor was it an infringement of Anwar's constitutional right to a fair trial.
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'Male Y' DNA in Mohd Saiful's rectum Anwar's, rules Federal Court
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*Solo* Double DNA w/ 105nl | Mein erstes Commentary – Video
Posted: February 10, 2015 at 11:44 am
*Solo* Double DNA w/ 105nl | Mein erstes Commentary
Bewerten und Kommentieren nicht vergessen! Schaut bei ihm vorbei: https://www.youtube.com/channel/UCNUBf_k5k_UKUG8qHHCwFfw.
By: Danex YT
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*Solo* Double DNA w/ 105nl | Mein erstes Commentary - Video
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*Live* The Revenge DNA sniper Mors & ASM1 | ArNova – Video
Posted: at 11:44 am
*Live* The Revenge DNA sniper Mors ASM1 | ArNova
Yo salut les gars ! 😀 Aujourd #39;hui je vous propose un dna en live au sniper mors + Asm1 sur la map bio lab Dna Live mors Dna Live mors Dna Live Sniper Advanced warfare Dna Live Sniper AW.
By: ArnovaHD || COD GTA || Subscribe (l)
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*Live* The Revenge DNA sniper Mors & ASM1 | ArNova - Video
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Tips & Tricks: AIM Verbessern in CoD Advanced Warfare | VIDEO der WOCHE | Dna Gameplay – Video
Posted: at 11:44 am
Tips Tricks: AIM Verbessern in CoD Advanced Warfare | VIDEO der WOCHE | Dna Gameplay
(How to) AIM Verbessern in CoD Advanced Warfare | VIDEO der WOCHE | Dna Gameplay | Tips und tricks ind Call of Duty Advanced Warfare Dna Gameplay Solar mit der HBRa3 im Spielmodus ...
By: PandaPlays
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Tips & Tricks: AIM Verbessern in CoD Advanced Warfare | VIDEO der WOCHE | Dna Gameplay - Video
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AW: "LIVE" *SOLO* DNA Bomb on 6v6 Riot Domination – XBOX 360 – Video
Posted: at 11:44 am
AW: "LIVE" *SOLO* DNA Bomb on 6v6 Riot Domination - XBOX 360
Leggete e sentite bene la intro, perch anche mettere il titolo non facile, senza commettere imprecisioni...spero che il gameplay vi piaccia, se cos, ne arriveranno altri anche migliori...
By: JTF Furin
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AW: "LIVE" *SOLO* DNA Bomb on 6v6 Riot Domination - XBOX 360 - Video
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INTENSE! CLUTCH "URBAN" DNA BOMB! COD: AW – LiVE! – (Advanced Warfare Havoc Multiplayer Gameplay) – Video
Posted: at 11:44 am
INTENSE! CLUTCH "URBAN" DNA BOMB! COD: AW - LiVE! - (Advanced Warfare Havoc Multiplayer Gameplay)
Call of Duty: Advanced Warfare - LiVE! CLUTCH "Urban" DNA BOMB! (COD: AW Havoc DLC Map Pack Multiplayer Gameplay) Tac-19 "Sledgehammer" Beastin! http://youtu.be/SQxPPlPLOS4 Clutch ...
By: TheRealTurboMan
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INTENSE! CLUTCH "URBAN" DNA BOMB! COD: AW - LiVE! - (Advanced Warfare Havoc Multiplayer Gameplay) - Video
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Immersed: Kevin Williams, Chairman of DNA – Video
Posted: at 11:44 am
Immersed: Kevin Williams, Chairman of DNA
Kevin Williams, Chairman of the DNA Association, talks about professional exhibition of immersive technologies like virtual reality at Immersed 2014.
By: Neil Schneider
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Immersed: Kevin Williams, Chairman of DNA - Video
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Basement Quality DateNight2015 – Gender Bender: DNA Twister Extreme – Video
Posted: at 11:44 am
Basement Quality DateNight2015 - Gender Bender: DNA Twister Extreme
Pat, Taha, and Nathan build a giant gender-swap machine for science. This is so deviantart it hurts. Follow us on Facebook for live streams and other announcements: https://www.facebook.com/Basem.
By: Basement Quality
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Basement Quality DateNight2015 - Gender Bender: DNA Twister Extreme - Video
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