Category Archives: DNA

Aw DNA W/ASM1 speakeasy
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Aw DNA W/ASM1 speakeasy - Video

Sex Lies And DNA
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The BioXp 3200 DNA workstation from SGI-DNA, a Synthetic Genomics subsidiary.

Advancing Craig Venter's vision of digitizing life, a company founded by the genome pioneer has started early sales of a new DNA workstation that assembles high-quality DNA sequences almost from scratch.

SGI-DNA, a subsidiary of Venter's Synthetic Genomics, announced this month it is selling its BioXp 3200 DNA workstation. It's intended for researchers who do a lot of work with DNA sequences, and who want to save time and improve accuracy by automating the process. The system cost just under $50,000 per machine, plus supplies.

As DNA sequencing becomes more widespread and less expensive, more sequence information becomes readily available. The BioXp device takes advantage of this trend to more efficiently construct sequences of interest. It includes an error-correcting process to boost accuracy.

Instead of isolating known DNA sequences from biological specimens, researchers can load the sequences into the BioXp to directly make the molecules, said Julie Robinson, senior product manager of synthetic biology at Synthetic Genomics.

Early buyers will also get assistance from SGI-DNA in assembling their DNA constructs, including custom synthesis directly by SGI-DNA for building long and difficult DNA sequences, the company says.

"We develop custom reagents for our customers, and they load the device, and it builds the DNA they can use in their studies and workflow," Robinson said.

DNA synthesis has been around for many years, supplied by companies like SGI-DNA. The BioXp provides this ability at the researcher's convenience, Robinson said. Instead of waiting for another company to supply the DNA, researchers can make it themselves when they need it. Or in other words, the instrument helps "democratize" DNA synthesis, she said.

"It helps to bring the workflow control to the customers themselves," Robinson said. "A lot of the tedious steps they have to do now will be automated."

The BioXp is the first step in that automation process, she said, and SGI-DNA plans to expand those capabilities so that steps such as DNA transcription and translation into proteins can be done with the instrument; steps that now generally use living cells.

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Synthetic Genomics launches DNA maker

Story highlights Scientists in southern Italy have known about him since 1993 Researchers worried that rescuing the bones would shatter them

The Altamura Man became the oldest Neanderthal to have his DNA extracted by researchers. It took them more than 20 years to get around to doing it.

Scientists in southern Italy have known about him since 1993, when spelunkers spied his skull staring blankly back at them from its nook in the Lamalunga cave, deep under the town of Altamura.

The cave explorers told researchers at the University of Bari what they'd found, according to their report published in March in the Journal of Human Evolution and Phys.org.

A man looks at an exhibit comparing modern humans to Neanderthals

Altamura Man's intact skull and jumbled pile of bones made for a great specimen, but they were wedged into a panoply of stalactites and stony globules deposited by water dripping over them for tens of thousands of years.

Researchers decided not to rescue the bones for fear that trying to ease them out of the cave's calcified grip would shatter them and ruin Altamura Man. So, they left him forever a cave man.

Calcite pebbles line the Neanderthal's eye sockets, nose bone and an upper jaw like a hundred decorative piercings. Analysis of the calcite has shown the bones to be 128,000 to 187,000 years old.

Scientists believe Altamura Man wound up in the cave at least that long ago after falling into a prehistoric well, then died in the caverns at its bottom of thirst or hunger.

Statue displays what scientists believe a Neanderthal man may have looked like

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Neanderthal who fell into well gives oldest DNA sample

IMAGE:This image shows (l-r) staff scientists Dr. Gil Amitai and Prof. Rotem Sorek. view more

Credit: Weizmann Institute of Science

It may come as a bit of a surprise to learn that bacteria have an immune system - in their case to fight off invasive viruses called phages. And like any immune system - from single-celled to human - the first challenge of the bacterial immune system is to detect the difference between "foreign" and "self." This is far from simple, as viruses, bacteria and all other living things are made of DNA and proteins. A group of researchers at the Weizmann Institute of Science and Tel Aviv University has now revealed exactly how bacteria do this. Their results were published online today in Nature.

"In most environments, phages are around ten times more abundant than bacteria. And, like all viruses, phages use the host cell's replication machinery to make copies of themselves," says Prof. Rotem Sorek of the Weizmann Institute's Molecular Genetics Department. "And they are constantly evolving new ways to do this. So bacteria need a very active immune system to survive."

But until recently, scientists were not even sure that bacteria had a so-called adaptive immune system - one that "remembers" a past encounter to produce a targeted response. That changed several years ago when a bacterial adaptive system called CRISPR was discovered. The CRISPR immune mechanism is not just crucial to the bacteria, it has a major impact on our daily lives: It is used today, for example, to protect the "good" bacteria that make yogurt and cheese. And it may also affect our future: Scientists have figured out how to use the ingenious CRISPR system to "edit" the human genome - making it a handy tool for a wide range of clinical applications.

To remember an infection, the CRISPR system grabs a short sequence from the invading viral DNA and inserts it straight into the bacterial genome. The bits of phage DNA are stored in special sections of the genome; these form the immune memory. In subsequent infections, CRISPR uses these sequences to create short strands of RNA that fit the genetic sequence of the phages' kin. Protein complexes attached to the RNA then identify the phage DNA and destroy it.

Selectivity is clearly an issue for such a system: Previous research in Sorek's lab had shown that mistakenly grabbing bits of self-DNA can cause the bacterial cell to suffer a sort of autoimmune disease in which it attacks its own DNA, and the results may be fatal to the bacteria. With around 100 times more self- than foreign DNA inside the cell, says Sorek, there would seem to be room for many more mistakes than researchers have actually observed.

How does the CRISPR system know how to insert foreign, rather than self, bits of DNA into the immune memory? Sorek and his research student Asaf Levy teamed up with Prof. Udi Qimron and Moran Goren of Tel Aviv University to answer the question in detail, revealing a complex, multi-step mechanism for this part of the CRISPR process.

They devised an experimental setup using plasmids - short, circular pieces of DNA that mimic viruses - and injected them into bacteria cells. These bacteria had two proteins known as Cas1 and Cas2 - parts of the CRISPR system that are responsible for acquiring the pieces of foreign DNA. The CRISPR system successfully incorporated the plasmid DNA into the bacterial genome, while the "self" DNA was only rarely attacked. The team recorded some 38 million separate immunization events.

Looking more closely at the results, the team found that the CRISPR system, using the proteins Cas 1 and 2, specifically identifies DNA that replicates rapidly. Thus, ironically, it is the phage's survival tactic - its programmed drive to replicate at all costs - that proves to be its downfall.

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How a bacterial cell recognizes its own DNA

Story highlights Scientists in southern Italy have known about him since 1993 Researchers worried that rescuing the bones would shatter them

The Altamura Man became the oldest Neanderthal to have his DNA extracted by researchers. It took them more than 20 years to get around to doing it.

Scientists in southern Italy have known about him since 1993, when spelunkers spied his skull staring blankly back at them from its nook in the Lamalunga cave, deep under the town of Altamura.

The cave explorers told researchers at the University of Bari what they'd found, according to their report published in March in the Journal of Human Evolution and Phys.org.

A man looks at an exhibit comparing modern humans to Neanderthals

Altamura Man's intact skull and jumbled pile of bones made for a great specimen, but they were wedged into a panoply of stalactites and stony globules deposited by water dripping over them for tens of thousands of years.

Researchers decided not to rescue the bones for fear that trying to ease them out of the cave's calcified grip would shatter them and ruin Altamura Man. So, they left him forever a cave man.

Calcite pebbles line the Neanderthal's eye sockets, nose bone and an upper jaw like a hundred decorative piercings. Analysis of the calcite has shown the bones to be 128,000 to 187,000 years old.

Scientists believe Altamura Man wound up in the cave at least that long ago after falling into a prehistoric well, then died in the caverns at its bottom of thirst or hunger.

Statue displays what scientists believe a Neanderthal man may have looked like

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Clumsy Neanderthal provides oldest DNA

Wendy Sherman deception is part of Iranian DNA Oct 2013
Wendy Ruth Sherman is Under Secretary of State for Political Affairs, the fourth-ranking official in the U.S. Oct 2013 At a meeting with US Senators on October 3, Sherman, who is the State...

By: ali javid

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Wendy Sherman deception is part of Iranian DNA Oct 2013 - Video

DNA BOMB ON TERRACE! // 43-2 (Call of Duty: Advanced Warfare)
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DNA BOMB ON TERRACE! // 43-2 (Call of Duty: Advanced Warfare) - Video

Under Progression #09 by Edgardo Di Giovanni @ DNA Radio [Abril.2015]
DOWNLOAD LINK TRACKLIST https://dl-web.dropbox.com/get/Edgardo%20Di%20Giovanni_Under%20Progression%20%2309_Abril2015.mp3?

By: Edgardo Di Giovanni

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Under Progression #09 by Edgardo Di Giovanni @ DNA Radio [Abril.2015] - Video

AK12 DNA BOMB ON SITE 244 NEW DLC! - Thanks for the support!

By: Guxsy - Pubstomper

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AK12 DNA BOMB ON SITE 244 NEW DLC! - Thanks for the support! - Video