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DUBLIN, Jan. 27, 2022 /PRNewswire/ -- The "Cystic Fibrosis (CF) Therapy Area Report" report has been added to ResearchAndMarkets.com's offering.

This Cystic Fibrosis (CF) therapy area report offers a comprehensive overview of the CF social media landscape, and provides insight into multiple stakeholder and company perspectives. Understand emergent signals and key trends amongst patients, caregivers, HCPs and digital opinion leaders, as well as the opportunities that exists for pharma to better engage with these groups.

The report, which is based on both machine and human-led analysis, uncovers the personas of online stakeholders, the key unmet needs, and opportunities for pharma to engage with the online customers. Insights from our report can help boost pharma companies' engagement strategies, identify potential opportunities and mitigate business risks.

Key Topics Covered:

CF Social Media Landscape Overview

The Patient Voice

Clinical Conversations

Competitive Intelligence

Companies Mentioned

For more information about this report visit https://www.researchandmarkets.com/r/2ftuiy

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Original post:

Cystic Fibrosis Therapy Area Report: Personas of Online Stakeholders, Key Unmet Needs and Opportunities for Pharma to Engage in 2021 - PRNewswire

A recent investigation into cystic fibrosis in children and young adults suggested that antimicrobial stewardship initiatives could be beneficial in standardizing care and fostering positive working relationships between cystic fibrosis pulmonologists, ID physicians, and pharmacists.

Cystic fibrosis has affected more than 30,000 individuals in the United States,with a major cause of morbidity and mortality being pulmonary disease and pulmonary exacerbations (PEx), the latter of which if often treated with antibiotics.

Despite the prevalence of PEx in patients with cystic fibrosis, there is no standardized definition to diagnose these events in affected patients, although most include an increase in respiratory symptoms and/or acute decrease in lung function.

Using the framework of antimicrobial stewardship, Geovanny F. Perez MD, MS, Division of Pulmonary Medicine, Oishei Children's Hospital,and fellow investigators aimed to develop an empiric antibiotic algorithm as a quality improvement initiative to initiative to develop consistency among providers in prescribing empiric intravenous (IV) antibiotic therapy for PEx events.

The quality improvement project was conducted at the Childrens National Hospital, a tertiary-care hospital with an accredited CF center.

Pediatric patients and young adults up to 26 years were admitted to the free-standing childrens hospital, and IRB approval for routine collection of demographic and clinical data around the time of PEx in persons with cystic fibrosis was obtained.

Participants 18 years and older provided written consent for the study, while written parental consent was obtained for patients under 18 years old, and assent was obtained from children between the ages of 11 and 17 years.

From there, the data acquired from each participant was used to assess baseline antibiotic use and the impact of the quality improvement initiative on IV antibiotics used for PEx treatment between January 1, 2017 and March 31, 2020.

Soon after, a multidisciplinary team of pulmonologists, infectious disease physisicans, and pharmacists with expertise in cystic fibrosis at Childrens National Hospital was assembled to develop the empiric antibiotic algorithm beginning in July 2017.

The quality improvement project assessed the antibiotic treatment courses of a total of 56 people with cystic fibrosis, with a total of 226 PEx episodes occurring during the study period.

Of those treatment courses, 25 were prescribed in the outpatient setting only (10 in the baseline period and 15 during the intervention), and the remaining courses were all initially prescribed in the inpatient setting.

Investigators observed that the mean age at the first PEx was 12 years old (SD 6.7). Regarding demographics, 55% of all participants who experienced PEx were female, 80% were white, and 71% were non-Hispanic.

After the initiation of the algorithm, the investigators noted that the proportion of PEx with antibiotic use consistent with the algorithm increased from 46.2% to 79.5%, and documentation of justification for broad-spectrum antibiotics increased from 56% to 85%.

Additionally, the use of infectious disease consults increased from 17% to 54%.

The team added that as young patients at their institution are introduced to effective CFTR modulators, the development of an algorithm for outpatient treatment could become an unmet need.

We would encourage other cystic fibrosis centers to explore their own trends in practice to determine whether a similar intervention may be both feasible and beneficial in the treatment of PEx in perosns with cystic fibrosis, the team wrote.

The study, "Measuring the Impact of an Empiric Antibiotic Algorithm for Pulmonary Exacerbation in Children and Young Adults with Cystic Fibrosis," was published online in Pediatric Pulmonology.

See original here:

Algorithm for Outpatient Treatment of Cystic Fibrosis in Young Patients Developed - MD Magazine

Significance

Submucosal glands secrete antimicrobial proteins and mucus into the airway lumen to protect the lung by killing inhaled and aspirated pathogens and clearing them from the lung. They can also contribute to several lung diseases, including the genetic disease cystic fibrosis. To better understand their structure and function, we isolated and studied submucosal glands from newborn pigs. Normal and cystic fibrosis submucosal glands were similar, suggesting that disease is due to loss of anion secretion rather than an intrinsic cell defect. By identifying submucosal gland cell types and the messenger RNA they express, the data aid understanding of submucosal gland function and provide a baseline for learning how environmental and genetic challenges contribute to lung disease.

Submucosal glands (SMGs) protect lungs but can also contribute to disease. For example, in cystic fibrosis (CF), SMGs produce abnormal mucus that disrupts mucociliary transport. CF is an ion transport disease, yet knowledge of the ion transporters expressed by SMG acini, which produce mucus, and SMG ducts that carry it to the airway lumen is limited. Therefore, we isolated SMGs from newborn pigs and used single-cell messenger RNA sequencing, immunohistochemistry, and in situ hybridization to identify cell types, gene expression, and spatial distribution. Cell types and transcript levels were the same in non-CF and CF SMGs, suggesting that loss of epithelial anion secretion rather than an intrinsic cell defect causes CF mucus abnormalities. Gene signatures of acinar mucous and acinar serous cells revealed specialized functions in producing mucins and antimicrobials, respectively. However, surprisingly, these two cell types expressed the same ion transporters and neurohumoral receptors, suggesting the importance of balancing mucin and liquid secretion to produce optimal mucus properties. SMG duct cell transcripts suggest that they secrete HCO3 and Cl, and thus have some similarity to pancreatic ducts that are also defective in CF. These and additional findings suggest the functions of the SMG acinus and duct and provide a baseline for understanding how environmental and genetic challenges impact their contribution to lung disease.

A major challenge for lungs is the continuous inhalation and frequent aspiration of microorganisms and foreign material. To meet this challenge, prevent infection, and clear particulates, the conducting airways have evolved a variety of defense mechanisms (17). In cartilaginous airways of humans, pigs, and other large mammals, submucosal glands (SMGs) are essential components of defense (1, 813). SMGs secrete liquid and most of the mucus in large airways; the secreted mucus binds microorganisms and particulate material, and ciliary beating propels the mucus out of the lung in the process of mucociliary clearance (1, 2, 5, 810, 13, 14). SMGs also produce abundant antimicrobial peptides and proteins that prevent infection by killing bacteria and inhibiting viruses (1, 11, 1518).

However, SMGs can also contribute to the pathophysiology of lung disease. In cystic fibrosis (CF), SMGs produce abnormally elastic mucus that disrupts mucociliary transport (14, 1924). In chronic obstructive pulmonary disease and asthma, SMGs hypertrophy and produce mucus that obstructs the airways (1, 2).

Despite their importance in maintaining healthy lungs and in contributing to lung disease, understanding SMG cellular architecture and molecular composition lags far behind understanding of surface epithelia that line the airways. Knowledge is limited in part because mice, the most commonly used laboratory mammal, lack SMGs except in the most proximal part of the airway (25, 26). SMGs isolated from humans are limited in availability and there is uncertainty about changes due to both apparent and unrecognized environmental and disease-related factors. SMGs are also relatively small and inaccessible, and while studies of electrolyte transport have been informative, they are limited to isolated serous cells (27, 28). Moreover, culture models have not replicated the three-dimensional structure of SMGs, which are organized into two regions, acini and ducts (29, 30). The acinus contains epithelial mucous cells that produce mucin and serous cells that secrete ions, liquid, and antimicrobials (1, 913, 16, 27, 28, 31, 32). Mucus flows from the acini to the airway surface through a series of ducts that merge to form a single terminal duct.

To better understand the cellular and molecular bases of SMG function, we studied the porcine lung. The size, anatomy, biochemistry, and physiology of the pig lung closely resemble those of the human lung (33). They have a similar amount and distribution of SMGs (26, 33, 34), and porcine SMGs have been used as a model in many physiological studies (21, 22, 35). Like humans, pigs have SMGs at birth, and by studying newborn pigs it is possible to avoid secondary manifestations of environmental exposures and disease. Pigs can also model human disease, including CF lung disease (3639). CFTRnull and CFTRF508 pigs develop airway infection, inflammation, and mucus occlusion much as seen in people with CF (19, 40, 41). Moreover, their SMGs exhibit duct obstruction and produce abnormally acidic mucus with increased elasticity that impairs mucociliary transport (18, 20, 22, 23, 42).

Here we investigated the single-cell transcriptome and cellular organization of SMGs from the newborn pig. We also tested non-CF and CF SMGs to learn if genotype-dependent differences in gene expression might explain differences in function.

We isolated SMGs from trachea of four wild-type and four CFTR/ newborn pigs. We removed the trachea, opened it along its ventral surface, scraped away most surface epithelial cells, and peeled the remaining tissue layer away from the cartilage (SI Appendix, Fig. S1). Fig. 1 A and B shows the SMGs and their structure composed of acini and ducts. The density and depth of SMGs would seem to fit well with the conclusion that the volume of glands is 50 times that of surface goblet cells (1). In addition, this layer included surrounding connective tissue and a few residual surface epithelial cells. We then manually dissected and isolated individual SMGs for single-cell dissociation.

scRNA-seq of newborn pig SMGs reveals multiple cell types. (A) Representative immunofluorescent image of SMGs after tissue was removed from the cartilage. Images were taken from the basolateral side of the tissue and stitched together. Staining is E-cadherin (green, an epithelial marker), WGA (red, a lectin interacting with MUC5B), and DAPI (blue). Structures with E-cadherin and WGA double-positive signals are SMGs. (B) Representative image of individual SMGs stained with E-cadherin and WGA in whole-mount SMG tissues. Acini are at the bottom and ducts are indicated with arrowheads. (C) UMAP of 14 cell clusters in scRNA-seq of SMG and surrounding tissues. Bas; basal cells; Cil, ciliated cells; Endo, endothelial cells; Fib, fibroblasts; Imm, immune cells; Muc, mucous cells; Myoep, myoepithelial cells; Myofib, myofibroblasts; Schw, schwann cells; Ser, serous cells; SM, smooth muscle cells. (D) Heatmap of top 10 cell-type signature genes. Representative markers from epithelial clusters are shown. Colors indicated scaled single-cell gene expression (maximum 200 cells were shown) at natural log scale.

We profiled 14,561 single-cells using droplet-based 3 single-cell RNA sequencing (scRNA-seq). We found that reads for many genes were not well aligned to reference genes, primarily because of the lack of 3 untranslated regions (UTR) of those reference genes (SI Appendix, Fig. S2 AD). To improve accuracy of the reference genome annotation, we manually checked sequencing read alignments for >6,000 genes that are expressed in human, pig, and mouse airways. We then modified the original RefSeq annotation by: 1) adding or elongating the 3 UTR, 2) reducing the overlap of the UTR region of two genes that have the same orientation, 3) updating gene names, and 4) adding new genes based on other pig genome databases. In addition, all modifications had to refer to existing annotations among University of California, Santa Cruz (UCSC), RefSeq, Ensembl, and GenBank messenger RNA (mRNA) databases. We modified annotations for 241 genes, 3.7% of the total genes that we checked (SI Appendix, Fig. S2 EG and Dataset S1). We generated a new pig reference genome and generated new matrix datasets based on it.

We subjected all filtered wild-type and CFTR/ datasets to integrative analysis with the Seurat R toolkit (SI Appendix, Fig. S3). We identified 14 major cell clusters (6 epithelial and 8 nonepithelial), which we visualized with two-dimensional uniform manifold approximation and projection (UMAP) (Fig. 1C and SI Appendix, Fig. S4A). Clusters contained as many as 5,469 and as few as 25 cells. We confirmed that cells were evenly distributed inside individual clusters regardless of genotype and batch (SI Appendix, Fig. S4B). We identified marker genes for each cluster based on unsupervised analysis (Fig. 1D, SI Appendix, Fig. S4C, and Dataset S2) and assigned each cluster to an existing cell-type in airways according to markers that are well known for a cell type (SI Appendix, Fig. S4D).

Cell-types included those classically reported for SMGs: serous, mucous, and myoepithelial cells (Fig. 1 C and D) (1, 32, 43, 44). We also identified basal and ciliated cells, which could potentially have derived from the airway surface. Three rare epithelial cell types have been reported in airway surface epithelia: tuft cells, ionocytes, and pulmonary neuroendocrine cells (PNECs) (4346). We detected PNECs and ionocytes but no tuft cells. In addition, there were eight nonepithelial cell types: endothelial cells, fibroblasts, myofibroblasts, two types of smooth muscle cells, two types of immune cells, and Schwann cells (SI Appendix, Fig. S4). These nonepithelial cells create the microenvironment for SMGs between the airway surface and the cartilage rings.

We compared data from wild-type and CFTR/ pigs. Marker-gene expression did not differ between cells from wild-type and CFTR/ SMGs (Fig. 2A). Moreover, the proportion of epithelial cell-types did not significantly differ by genotype (Fig. 2B), although there were more epithelial cells in the wild-type dataset (SI Appendix, Fig. S4B). In addition, within SMG-specific cell types, only a few genes showed differential expression between wild-type and CFTR/ (Fig. 2C and Dataset S3). qRT-PCR of multiple genes identified by scRNA-seq revealed only one differentially expressed gene (zymogen granule protein 16B, P < 0.05) between the two genotypes (Fig. 2D). These results suggest that wild-type and CFTR/ SMGs have a similar cellular and molecular composition. In subsequent analyses, we combined data from all eight pigs.

Wild-type and CFTR/ SMGs exhibit similar gene expression profiles. (A) Dot plots of selected marker genes that were enriched in each cell type separated by genotype. y axis, cell types; x axis, mean expression of indicated marker-gene sets. The size of the dot indicates the proportion of cells that were positive for the gene sets in specific cell-types. Color of dots indicates the expression of the gene sets (scaled by row) at natural log scale. (B) Bar graph of epithelial cell-type proportion in wild-type and CFTR/ SMG. (C) Volcano plots of differentially expressed genes in serous, mucous, and myoepithelial cells between wild-type and CFTR/ SMGs. LogFC indicates fold-change between wild-type and CFTR/ at natural log scale. Dashed lines indicate the cutoffs: genes that were 1 LogFC higher or lower with false-discovery rate (FDR) < 105 in wild-type are highlighted in red. (D) qRT-PCR analysis of serous and mucous cell markers and differentially expressed genes between wild-type and CFTR/ from C. CT values for each gene were normalized to the CT value of EPCAM. Primers for CFTR targeted the deleted exon in CFTR/ pigs (36). Each dot represents a pig; P values were generated using unpaired Students t test.

SMGs were organized with multiple acini connecting to a single duct (Fig. 1B and SI Appendix, Fig. S5). To reconstruct the spatial distribution of cell types and the architecture of SMGs, we used immunostaining and single-molecule fluorescence in situ hybridization (smFISH) with representative markers.

Acini were composed primarily of three cell types: serous, mucous, and myoepithelial cells. Serous cells were positive for BPIFA1 (PLUNC), DMBT1, OBP2B, and mucous cells were positive for MUC5B and MUC19 (Fig. 3 AC). In general, serous cells were mixed with mucous cells throughout most of the acinus. Occasionally serous cells formed a cluster at the very distal part of an acinus, but we did not find areas with only mucous cells (Fig. 3C, SI Appendix, Fig. S5, and Video S1). This organization differs from a report of human SMGs, in which serous cells were located in the distal portion of an acinus branch, and mucous cells clustered in the proximal portion of acinus branches (29).

SMG acini contain a mix of serous and mucous cells covered with myoepithelial cells. (A) Representative image of SMG immunostained for of PLUNC (BPIFA1) and MUC5B. (B) Representative image of smFISH of serous cell (DMBT1, OBP2B) and mucous cell (MUC19) markers in SMG acinus. (C) Analysis of cellular composition of SMG acinus. (Left) Representative image of smFISH of DMBT1 (serous cell marker) and MUC5B (mucous cell marker) in SMG acini. (Right) Summary of acinar sections containing pure serous cell population, pure mucous cell population, or a mix of these two cell types. Each data point represents an image. Statistical comparison was based on averaged values of multiple images from a pig; n = 4 pigs, unpaired Students t test. (D) Representative images of smFISH of myoepithelial cell (ACTA2, KRT5, PCP4) markers in the acinar region of SMGs. (E and F) Representative images of immunostaining of SMA (protein name for ACTA2) in SMG tissue sections and whole tissue. SMA was localized in the acini, not the duct of SMGs. BV, blood vessel; E-cad, e-cadherin, labeled the entire population of epithelial cells in SMGs; SM, smooth muscle. (G) Model of spatial distribution of epithelial cell types from scRNA-seq. Each dot represents a nucleus from a real tissue section. Colors for epithelial cell types are matched to cell types in Fig. 1C. ASE, airway surface epithelia.

Myoepithelial cells were positive for ACTA2, KRT5, and PCP4, a new marker for myoepithelial cells (Figs. 1D and 3D and SI Appendix, Fig. S6). We found that myoepithelial cells wrapped around the acinus of SMGs (Fig. 3 E and F). Previous reports suggest that myoepithelial cells of airway SMGs facilitate gland contraction and mucus secretion in response to neural signals (1, 47). Consistent with that suggestion, their shape and gene-expression signature suggested contractile features.

These findings identify and localize cell types in the porcine SMG acinus (Fig. 3G).

To further predict distinct and shared functions of SMG serous and mucous cells in host defense, we compared their levels of transcripts. We curated a set of host defense-related genes (Dataset S4), and found that serous and mucous cells were specialized for some host defense-related genes. For example, mucous cells expressed MUC5B and MUC19 mucin genes at much higher levels than serous cells (Fig. 4A). Also as expected, compared to mucous cells, serous cells were enriched for transcripts for several secreted proteins, including secretory leukocyte peptidase inhibitor (SLPI), lactoferrin (LTF), and bactericidal permeability increasing proteins (BPI variants) (Fig. 4 A and B). However, the distinction was not absolute: mucous cells also expressed some of these host defense genes. As an example, mucous cells preferentially expressed BPIFB2 (Fig. 4 A and B). Moreover, an antimicrobial usually ascribed to serous cells, lysozyme (LYZ), unexpectedly showed fourfold greater expression in mucous cells (Fig. 4 A and C).

Acinar serous and mucous cells have distinct expression patterns of host defense genes. (A) Scatter plot of mean expression of host defense genes in mucous and serous cells at natural log scale. The dots for labeled genes are enlarged for better visualization. (B) Representative images of smFISH of LTF, DMBT1, and BPIFB2 in SMGs. LTF colocalized with DMBT1. (C) Representative images of smFISH of LYZ in SMGs. LYZ colocalized with MUC5B signals.

To better understand the potential role of the two acinar cell types in electrolyte and liquid secretion, we also curated a set of genes that encode proteins involved in transepithelial electrolyte and water transport (Dataset S4). We were surprised to find that serous and mucous cells had similar transcript levels for genes involved in anion secretion, including apical anion channels TMEM16A (ANO1) and CFTR; basolateral anion transporters including the Na+/K+/2Cl cotransporter (NKCC), the Na+/H+ exchangers (NHE1 and NHE8), the Na+/HCO3 cotransporter (NBC1, NBC3), plus carbonic anhydrases (CA9 and CA12) that generate intracellular HCO3 (Fig. 5 A and D). In addition, both cell types expressed similar transcript levels for a basolateral (AQP3) and an apical (AQP5) aquaporin, the Na+-K+-ATPase, and K+ channels (Fig. 5 AC and SI Appendix, Fig. S7). Moreover, the cell types contained similar mRNA levels for gap junction genes, suggesting coupling between the two cell types (Fig. 5E).

Acinar serous and mucous cells express similar transcripts for ion transporters. (A) Scatter plot of mean expression of transepithelial ion transporters in mucous and serous cells at natural log scale. Different colors represent different subcellular localization of indicated transporters. (B and C) Representative images of aquaporin staining in SMG acini. In B, AQP5 is equally distributed in BPIFB2+ (mucous) cells and BPIFB2 (serous) cells. In C, AQP3 protein is localized on the basolateral side of all acinar cells of adult SMGs. (D) Representative images of the expression pattern of key ion transporters (CFTR, TMEM16A, NKCC) in the acinus of SMGs. Serous cells (DMBT1+) are shown in green; mucous cells (MUC5B+) are shown in red. (E) Scatter plot of the expression of gap junction genes in serous and mucous cells. The line across the plot indicates equal expression of genes between serous and mucous cells. (F) Scatter plot of mean expression of secretory-related receptors in mucous and serous cells at natural log scale. (G) Representative image of smFISH of CHRM3 (gene for muscarinic cholinergic receptor M3) in SMG acini. DMBT1 (green) and MUC5B (gray) are markers for serous cells and mucous cells, respectively.

Secretory activity will depend, in part, on plasma membrane receptors that sample neurohumoral signals. We did not identify a receptor that was unique to either serous or mucous cells; both expressed receptors for acetylcholine, substance P, VIP, histamine, and adrenergic agonists (Fig. 5 F and G).

We noticed that when we labeled with two serous cell markers, not all DMBT1+ cells were also positive for OBP2B (Fig. 3B). Therefore, to assess heterogeneity of serous cells, we reanalyzed the large serous cell cluster and identified four subclusters (Fig. 6 A and B). A subcluster of ductal serous cells had unique gene expression features (Fig. 6 C and D, SI Appendix, Fig. S8, and Datasets S5 and S6) and localized along the inner layer of SMG ducts. Ductal serous cells expressed markers that were common in other serous cells, such as DMBT1 and BPIFA1 (PLUNC), but they did not express some markers typical of acinar serous cells, such as OBP2B and BPIFB5 (Fig. 6 BE and SI Appendix, Fig. S8B). In addition, ductal serous cells expressed some genes expressed by airway surface secretory cellssuch as LCN2, SCGB3A1, SLPI, and MUC5ACbut not others, such as S100A8, S100A9, and S100A12 (Fig. 6 B, C, and EH). Thus, ductal serous cells share some features with acinar serous cells and surface secretory cells; however, they differ from both. Consistent with these findings, we detected secretory vesicles in SMG duct cells (SI Appendix, Fig. S9). We also localized the basal cells to the outer layer of SMG ducts (SI Appendix, Fig. S10), basal cells, and ductal serous cells form the SMG duct epithelia.

SMG ducts have a unique subcluster of serous cells. (A) Subclusters from the UMAP serous-cell cluster in Fig. 1C. The serous-cell cluster was further grouped into four subclusters. Subclusters include two types of acinar serous cells (Ser1, Ser2), serous cells in division (Cycling Ser), and ductal serous cells (Ductal Ser). (B) Expression pattern of representative markers for acinar serous cells and ductal serous cells in UMAP. (C) Volcano plot of genes differentially expressed (red) between ductal serous cells and acinar serous cells (Ser1). (D) Representative images of immunostaining of PLUNC (BPIFA1) in the duct of newborn (Left) and adult (Right) SMGs. (E) Representative images of smFISH of secretory genes (DMBT1, OBP2B, MUC19, LCN2, SCGB3A1, WFDC2, MUC5B) in the duct of SMGs. (F) Representative images of smFISH (Left) and immunostaining (Right) of MUC5AC in the duct of pig SMGs. (G) Representative images of immunostaining of MUC5AC in the duct of human SMGs. (H) Representative image of smFISH of S100A9 (a marker for airway surface secretory cells) in airway surface epithelia and SMGs. The SMG duct and acinus did not express S100A9.

Our scRNA-seq data also identified some ciliated cells, and previous studies reported ciliated cells in human SMG ducts (29, 48). However, smFISH and immunostaining for three ciliated cell markers, FOXJ1, TMEM212, and acetylated -tubulin, localized them exclusively to surface epithelia with none in SMG ducts (SI Appendix, Figs. S11 and S12). We also examined airways with immunohistochemical staining for acetylated -tubulin in older pigs and found that the pseudostratified surface epithelium with ciliated cells sometimes dipped down in toward the SMG duct before the duct developed a cuboidal structure (SI Appendix, Fig. S12). However, the cuboidal duct epithelium did not contain ciliated cells in newborn or adult pigs. Because this result contrasts with data from human SMG ducts (29, 48), as a control we reproduced that finding by obtaining human tissue and detecting FOXJ1+ ciliated cells in SMG ducts (SI Appendix, Fig. S13). Thus, ciliated cells in our scRNA-seq data were from the airway surface and the pseudostratified epithelium at the most proximal portion of the duct, and in contrast to human SMG ducts, the cuboidal epithelia of pig SMG ducts do not contain ciliated cells.

Although ionocytes were rare in the scRNA-seq data, immunostaining for BSND, a marker for ionocytes, revealed ionocytes in SMG ducts (SI Appendix, Fig. S14). These results are consistent with previous reports that CFTR-expressing ionocytes are located in the ducts (43, 49).

To further assess potential contributions to ion and water secretion, we examined the curated set of genes involved in transepithelial electrolyte and water transport (Fig. 7A and Dataset S4). Ductal serous cells express channels and transporters that enable transepithelial HCO3 and Cl secretion; these include NKCC, AE2, NBC3 that transport anions across the basolateral membrane, and TMEM16A and CFTR that allow anion exit from the cell across the apical membrane (Fig. 7 A and B). Also consistent with an epithelial transport function, ductal-serous cells expressed both basolateral (AQP3) and apical (AQP5) aquaporins (Fig. 7 A, C, and D). Expression of these genes encoding transporters involved in anion and liquid secretion were similar in ductal and acinar serous cells. Also consistent with a secretory rather than an absorptive function, we detected transcripts for ENaC but not the other required subunits (Fig. 7 A and E); ENaC is a key channel for Na+ absorption in airway surface epithelia (50).

SMG ductal serous cell transcripts indicate an anion secretory function. (A) Scatter plot of the mean expression of transepithelial electrolyte transporter genes in ductal and acinar serous cells at natural log scale. Different colors of dots indicate different subcellular localization of the encoded protein. (B) Representative images of the expression pattern of key ion transporters (CFTR, TMEM16A, NKCC, NBC3) in the duct of SMGs. Ductal serous cells (DMBT1+) are shown in red. (C) Representative image of smFISH of AQP3, AQP5, and KRT14 in tracheal sections. In SMGs, AQP3 and AQP5 are expressed in both ductal serous cells and acinar serous cells. KRT14 is expressed in basal cells of the duct and myoepithelial cells of the acinus. (D) Representative images of immunostaining of AQP3 in airway sections of an adult pig. Enlarged image shows that AQP3 is localized at the basolateral region of ductal serous cells. (E) Representative images of smFISH of SCNN1B (encodes the -subunit of ENaC) in tracheal sections. SMG duct (DMBT1+ region, red) was not labeled with SCNN1B signal.

We studied porcine SMGs at single-cell resolution with RNA sequencing, smFISH, and immunohistochemistry to construct SMG molecular signature and infer function. The data support and expand previous observations and interpretations. However, the results also provide new insight into the molecular architecture and potential cell-typespecific functions.

Substantial functional evidence has assigned anion and liquid secretion to serous cells (1, 27, 28, 51). Consistent with previous evidence that mucous cells can contribute to C1 secretion (1), our findings suggest that mucous cells secrete anions and liquid. We found that mucous cells express the same transcripts at approximately the same levels as serous cells for proteins that mediate anion secretion. These include the apical anion channels CFTR and TMEM16A (52, 53) and the basolateral NKCC and NBC1 and NBC3. Both cell types express cytosolic carbonic anhydrases (CA8, CA12, and CA9) that couple with basolateral NHE8 and NHE1 to generate HCO3 for secretion. For water transport, both cell types express apical and basolateral aquaporins (AQP5 and AQP3). In addition, both cell types express gap junction proteins, suggesting that the cells are coupled so that the electrical driving forces across their basolateral and apical membranes may be the same. Moreover, both cell types express a similar group of receptors that may stimulate secretion.

These results suggest that both mucous and serous cells mediate Cl and HCO3 secretion and raise the possibility that the two cell types may make relatively comparable contributions. Consistent with this conclusion, it is reported that substance P causes rapid cell shrinkage, an assay of anion secretions, and at the end of stimulation, both cell types quickly restore their volume (1, 54). We speculate that when SMGs are stimulated, this arrangement of ion transporters, aquaporins, gap junctions, and receptors may serve to ensure a balance between the amount of secreted liquid, the concentration of protein, and the pH in the acinar lumen. Those properties are critical for producing mucus with normal biophysical properties and for ensuring activity of antimicrobial peptides and proteins (18, 20, 22, 55, 56).

In addition to providing a conduit, gland ducts often modify their contents. For example, in the sweat gland, ducts absorb Na+ and Cl, and in the pancreas, ducts secrete HCO3 (5759). CF disrupts the function of both of these ducts. Whether or how SMG ducts modify mucus flowing to the airway lumen is uncertain. Previous reports about whether SMG ducts express ENaC Na+ channel subunits vary (6062). An extracellular electrophysiological study suggested that porcine SMG ducts absorbed Na+ and Cl and reduced the Na+ and C1 concentrations of secreted liquid (63). Another study indicated that SMG ducts do not alter the liquid volume of the duct lumen (64). Moreover, Widdicombe and Wine (1) concluded that ion transport by duct epithelia was very unlikely to alter duct liquid volume given the trivial area of ductal epithelia vs. the area of acinar epithelia.

We found that transcripts for , , and ENaC Na+ channel subunits were absent or of low abundance in duct serous cells, although they may have been below our level of detection. However, duct serous cells expressed multiple genes required for transepithelial anion secretion. These results suggest that porcine SMG ducts have a secretory function, and we speculate that HCO3 secretion is the main transport function of the ducts. This hypothesis is also based on the observation that the pH of liquid emerging from SMG ducts is 7.5 (22, 55). That pH value puts the HCO3 concentration above equilibrium for a basolateral pH of 7.4. In addition, an electrically negative lumen places the HCO3 concentration substantially above electrochemical equilibrium. In the pancreatic duct, HCO3 secretion is supported by Cl exit through CFTR channels followed by Cl reentry in exchange for HCO3 exit on the SLC26A6 Cl/HCO3 exchanger (65). A similar mechanism may exist in serous duct cells, which also express SLC26A6.

SMGs produce mucus with a MUC5B core coated with MUC5AC (14, 23). Acinar mucous cells produce the MUC5B core, and we speculate that as it flows through the duct, serous cells secrete MUC5AC that coats the MUC5B core. Of course, MUC5AC derived from surface goblet cells could also coat the strands. The reason for this arrangement of mucins is unknown and selective properties of different gel-forming mucins remain uncertain. We wonder if MUC5B might be more important for the elasticity and toughness of mucus strands, and MUC5AC might be more important for adhesiveness and binding microbes and particulate material.

Previous examinations have revealed mucin production as a major function of acinar mucous cells and antimicrobial protein/peptide production as a major function of acinar serous cells (1, 12, 32). Our results are consistent with those contributions to respiratory host defense. For example, mucous cells expressed higher mRNA levels for the mucins MUC5B and MUC19, and acinar serous cells expressed higher mRNA levels for antimicrobial proteins lactoferrin, secretory leukocyte peptidase inhibitor, and bactericidal permeability increasing proteins. However, these distinctions were not absolute; for example, mucous cells had more transcripts for the antimicrobial lysozyme than did serous cells, which differs from human SMGs in which serous cells secrete lysozyme (66). Secretion of both mucins and antimicrobial proteins into the same small acinar lumen raises the question of whether the two might interact with antimicrobials decorating mucins, perhaps based on the charge of the negative mucins and the positive antimicrobials. Such an interaction might facilitate delivery or localization of the antimicrobials (67).

Mucous produced by human SMG acini is reported to empty into an enlarged duct, called a collecting duct, before exiting through a long thin duct to the airway surface (29). Porcine SMGs did not have a well-defined collecting duct. However, a review of SMG structure suggests that the presence of collecting ducts is variable in humans and other species (48). It is also possible that a region resembling a collecting duct may develop in older pigs (68).

In porcine SMG ducts, the pseudostratified surface epithelium with its ciliated cells dips down into the duct for a very short distance and then immediately transitions to duct serous and basal cells lacking cilia. This differs from human SMG ducts, which are ciliated. The presence or absence of ciliated cells may relate to the length of ducts. In pigs, SMGs are localized between the cartilage layer and airway lumen (8). However, in humans, SMGs sometimes invaginate deep beneath the airway lumen, extending between cartilage rings (29). We speculate that beating cilia may be required to pull mucus up the long ducts in humans. In contrast, pressure from mucus and liquid secretion and contractile activity of myoepithelial cells may be sufficient to push mucus up porcine SMG ducts.

Despite the structural differences between human and pig SMGs noted above, in both humans and pigs, CF SMG produce mucus strands having abnormally increased elasticity that prevents normal mucociliary clearance and the ducts are plugged with mucus (19, 20, 22, 23, 42, 56).

This study has advantages. First, studying freshly dissected SMGs rather than performing scRNA-seq on cells from full-thickness airway tissue minimizes the necessity of using transcripts to infer a SMG location. Second, studying SMG cells obtained directly from animals avoids the uncertain effects that culturing can have on cell types and transcript profiles. Third, our conclusions are based on a combination of data from scRNA-seq, in situ hybridization, immunohistochemistry, and histology. Fourth, studying SMGs from newborn pigs avoids potential confounding influences of environmental factors, inflammation, infection, and disease.

This study also has limitations. First, we show that porcine and human SMGs have some differences. However, the differences are limited, and lungs and SMGs of pig and human share many features (33). Moreover, the biochemistry, physiology, and anatomy of the porcine lung are similar to those of humans. Second, as is common in scRNA-seq studies, the proportion of various cell types can be biased by differential isolation, and varying dissociation, viability, and lysis that can misrepresent the true proportion of the various cell types. Fortunately, our main conclusions are not dependent on the relative proportions of the various cell types.

By studying SMGs in newborn pigs, we were able to test for CF vs. non-CF differences prior to the onset of infection and inflammation (40). At that time point, the cell-type composition and transcripts did not differ by genotype. This result is consistent with previous mRNA microarray (69, 70). Lack of substantial differences between CF and non-CF SMG transcripts are consistent with the observation that early CF host-defense defects in antimicrobial activity and mucociliary clearance are reproduced simply by preventing Cl and HCO3 secretion; these interventions that acidify the pH, decrease liquid secretion and increase protein concentration, functions mediated directly by CFTR anion channels (18, 20). In contrast, a Ca2+-activated Cl channel, TMEM16A, may have cell-intrinsic function, as TMEM16A gene disruption alters airway development and barrier function (71).

SMGs are essential for normal respiratory host defense in humans and large mammals (1, 8). These data enlarge our knowledge of these structures, identify their cellular and molecular composition, and provide a baseline for understanding how disease and environmental challenges alter the lungs.

A more detailed version of methods is provided in SI Appendix.

Human lung tissues were obtained from the Iowa Donor Network through the InVitro Models and Cell Culture Core of the University of Iowa. All studies were reviewed and approved by the Animal Care and Use Committee of the University of Iowa.

Wild-type and CFTR/ pigs (36) were generated and delivered by Exemplar Genetics. Newborn pigs for tissue harvest were 8 to 15 h after birth. They were anesthetized and killed. Tracheal segments were harvested and kept in cold 1 DPBS before further procedure.

SMG and surrounding tissues were dissected from the trachea of newborn pigs (Dataset S7). Before dissection, airway surface epithelial cells were scraped off and the remaining epithelial layer was separated from the cartilage layer of the trachea to reveal SMGs. Dissected SMGs were successively dissociated with collagenase/hyaluronidase and pronase/DNase medium at 37C. After that, dissociation was terminated using cold 1 DPBS with fetal bovine serum. Cells were further washed with cold 1 DPBS and filtered with 40-m tissue strainers. Single cells were spun down and resuspended in 1 DPBS with 0.4 mg/mL bovine serum albumin at around 1,000 cells/Ll. Single-cell droplets were generated using 10X Genomics Chromium Controller in the core facility of the Iowa Institute of Human Genetics of the University of Iowa. Next, 5,000 cells were targeted for single-cell droplets. cDNA libraries were generated using the Chromium Single Cell 3 Reagent Kits v2 system. cDNA libraries passed all the quality controls that were recommended by 10X Genomics before sequencing. Sequencing was conducted on a HiSEq. 4000 system.

Raw sequencing FASTQ data were converted to matrix data (counts for genes) using the Cellranger 3 toolkit. An updated RefSeq Sscrofa11.1 genome annotation was used as the genome reference. Matrix data were subjected to ambient RNA correction using the SoupX R package (72), doublet filtering using the Scrublet Python package (73), and dead and low-quality cell filtering in Seurat (74).

Single-cell datasets were integrated and clustered using the Seurat 3.1.4 R package (74) following the typical integration tutorial (https://satijalab.org/seurat/v3.1/integration.html). In brief, matrix data were normalized at natural log scale using scale factor as 10,000. Two-thousand variable features were automatically selected for dataset integration. After integration, datasets were scaled regressing out percent.mt and cell-cycle identities, following principal component analysis with the first 30 principal components. Cell clusters were visualized in two-dimensional UMAP (75).

Marker genes for each cluster were calculated using the function FindAllMarkers in Seurat. The Wilcoxon rank sum test was used. Testing was limited to genes that were expressed in more than 25% of a cell population and were differentially expressed on average at least 0.25-fold at natural log scale. Clusters were named based on specific marker genes that were previously reported for a certain cell-type in previous studies.

Differential expression analysis between wild-type and CFTR/ was carried out using the function FindMarkers in Seurat. The Wilcoxon rank sum test was used, logfc.threshhold was set to 0.25.

Formalin-fixed paraffin-embedded sections, cryosections, and whole tracheal epithelial tissues were processed following protocols described in SI Appendix, Supplemental Methods. Sections and tissues were preblocked with 10% serum before primary antibody incubation. The information of primary antibodies is listed in Dataset S8. After primary antibody incubation, sections and tissues were washed with 1 DPBS and incubated with Alexa Fluor secondary antibodies. Sections and tissues were mounted in antifade mounting medium (# H-1500, Vector laboratories) and imaged with confocal microscopy.

An smFISH method named proximity ligation in situ hybridization was performed based on a published protocol (76) with a few modifications. In brief, tracheal segments were fixed in 4% formaldehyde, and 10-m-thick cryosections were collected and preserved at 80C before in situ hybridization. On the day of in situ hybridization, tissue sections were postfixed in cold 4% PFA for 20 min. Sections were then incubated in prewarmed citrate solution with 0.05% lithium dodecyl sulfate and 0.05% Triton X-100 at 65C for 30 min. After that, sections were incubated with 0.05 mg/mL pepsin in 0.1N HCl for 10 min at 37C following 1 DPBS washing. At this stage, typical proximity ligation in situ hybridization was performed on hybridization-ready sections. The short mRNA sequences that were targeted by the paired hybridization probes are listed in Dataset S8.

Tracheal segments (<1 mm3) from newborn pigs were cut, briefly rinsed in cold 1 PBS, and fixed in glutaraldehyde fixation buffer (2.5% glutaraldehyde, 0.1 M sodium cacodylate). Tissues were next postfixed in osmium tetroxide (2%), and en-bloc stained with uranyl acetate (2.5%). Then, tissues were dehydrated through a graded ethanol series, infiltrated with Eponate 12, and polymerized at 60C for 24 h. Next, 70-nm-thick sections were cut and counterstained with 5% uranyl acetate and Reynolds lead citrate. Images were taken using a transmission electron microscope (JEOL, JEM1230) with a Gatan UltraScan 2k 2k CCD camera.

Fluorescent images were taken using the Olympus FV3000 system. Individual field or multiple fields of z-stack images were scanned and stitched together. Images were processed to either single z-stack images or z-stack videos.

Data points in graphs represent biological samples unless specifically indicated in the text. Results in graphs are presented as mean SD. Statistical significance was tested using Students t test, P values less than 0.05 were considered statistically significant.

We thank Monica Nagendran and Tushar J. Desai from Stanford University for sharing the in situ hybridization protocol with us; people from the cystic fibrosis animal core, the InVitro Models and Cell Culture Core, the sequencing core of the Iowa Institute of Human Genetics, and the Central Microscopy Research Facility of the University of Iowa for experimental assistance. This work was funded by the NIH (HL091842, HL051670), a Cystic Fibrosis Foundation Research Development Program, and the Roy J. Carver Charitable Trust. M.J.W. is an Investigator of the Howard Hughes Medical Institute.

Author contributions: W.Y., D.A.S., and M.J.W. designed research; W.Y., T.O.M., Y.X., K.Z., and L.S.P. performed research; W.Y., A.L.T., A.J., A.A.P., and M.J.W. analyzed data; and W.Y. and M.J.W. wrote the paper.

Reviewers: L.J., HHMI, University of California, San Francisco; and J.W., University of California, Davis.

Competing interest statement: The University of Iowa Research Foundation has licensed intellectual property related to gene modified pigs to Exemplar Genetics. Royalties from that license are shared with M.J.W. and D.A.S. Neither M.J.W. nor D.A.S. have other financial ties to Exemplar Genetics.

This article contains supporting information online at https://www.pnas.org/lookup/suppl/doi:10.1073/pnas.2119759119/-/DCSupplemental.

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Cellular and molecular architecture of submucosal glands in wild-type and cystic fibrosis pigs - pnas.org

People with cystic fibrosis (CF) can have a complicated relationship with food and eating that changes over their lifetime, a series of patient interviews regarding diet reveals.

For many patients, bad childhood experiences needing feeding tubes and having to pack on more weight impacted their perceptions of diet and eating.

The interviewees also shared strategies for getting enough calories, including choosing high-calorie foods and getting help from friends and family.

The results were published in the study, Perception, experience and relationship with food and eating in adults with cystic fibrosis, in the Journal of Human Nutrition and Dietetics.

CF can have substantial impacts on the digestive system, and many patients will eventually develop CF-related diabetes and/or exocrine pancreatic insufficiency (EPI), a condition in which the pancreas cant release enough digestive enzymes into the gut.

Dietary and eating habits can therefore be crucial for managing CF; for example, patients are often encouraged to eat a high-calorie diet to ensure that they get enough nutrients to power their bodies. However, there is little published research on attitudes toward food and eating among people living with the disease.

Now, researchers in the U.K. conducted interviews with nine adults with CF, ranging in age from the early 20s to the late 40s. All patients had EPI, most had diabetes, and there was a roughly even mix of sexes. Each of the interviews lasted about 20 to 30 minutes.

Despite patients undergoing regular surveillance of their nutritional status and dietary management being a prominent aspect of their CF treatment, this is one of the first studies to specifically explore the perceptions of food and eating experienced by adults with CF, the researchers wrote. The team noted previous generalized patient accounts that eating often felt like just another necessary treatment.

One theme that emerged from these new discussions was that many adults recalled bad experiences with food as a child for example, being picky eaters or feeling that they had no appetite but that these problems tended to get better as they entered adulthood and had more freedom and control over what they ate. One participant said that meeting his wife had helped him expand his dietary horizons, because we used to go out [for] meals and then I wanted to try new things.

Several participants recalled needing to get nutrition from a feeding tube as children, and said that avoiding the need to do so again helped motivate them to keep up good dietary habits.

I didnt eat very well when I was little and I had to have nasogastric tube feeding [a narrowfeeding tube placed through the nose down into the stomach] for 2 years as I got older it got scary. If I didnt put weight on then I would have to be tube fed I always looked on tube feeding as something to try to avoid, one patient said.

The researchers suggested that, in counseling adults with CF about tube feeding or other measures, such past experiences should be explored, as they may have a powerful influence on patient perceptions.

Generally, eating enough to get sufficient nutrition and maintain a healthy body weight was viewed as a priority by the patients. In fact, for some, eating a high-calorie CF diet could help them feel fortunate relative to their non-CF peers.

When my weight is healthy I dont tend to get as many infections and stuff, yeah, its all part and parcel of staying healthy, one interviewee said.

I feel like where my mates put restrictions on what they can or cant eat I dont I just eat what I want, said another.

However, for some patients, the necessity of consuming calories could sap the pleasure out of eating. For instance, one patient said, its not that I dont enjoy eating its that Im not hungry, because its time to eat and because I want to keep on top of my weight and health.

Additionally, while patients generally understood the importance of keeping up a healthy body weight, they also voiced concerns about gaining too much weight and feeling uncomfortable with the image of their bodies.

None of the patients reported dieting, although some did [describe] manipulating their diet to control their weight, balancing this between achieving a beneficial weight for their health and being satisfied and comfortable with their body image, the researchers wrote.

The interviewees noted several strategies for keeping up a high-calorie diet, such as bigger portions, including a host of side dishes for variety, and sticking to routines.

If I go for a meal I will have pasta and then say can I get sides with that or can I have chips or maybe another side like garlic bread adding to it. Thats how I usually think when I am choosing something can I add a little bit more to it, said one patient.

Another recommended choosing higher-calorie food alternatives where possible: Even with smaller things like butter and that I always get proper butter rather than the low fat spreads and stuff I just think its just another way of getting calories in without having to eat more.

Support from friends, family, and healthcare providers also was crucial for keeping up a good diet.

My partner knows, after a long day at work I get home and just have a bowl of cereal she will say thats not enough you need to eat more food, one patient said. Another reported, When my mum goes out she always comes back with some chocolate, she is always plying me with high calorie snacks.

I can go a few days without even thinking about food, another patient said. Friend and my mom then say have you actually eaten anything today and they get me something, otherwise I wouldnt bother.

The researchers said the interviews show the need for clinicians to take experiences from patients childhood into account when helping to create effective dietary plans.

It is important that past experiences with food and eating including childhood experiences and body image perceptions are explored during dietetic consultations, as these can have a sustained effect on eating behaviour in adulthood, the team wrote. Identifying motivations and barriers to dietary change, and aspects of food and eating that are important to patients can aid the success of dietetic therapy and ensure it is patientcentred.

Link:

Patient Interviews Reveal Complicated Relationship With Food and Diet - Cystic Fibrosis News Today

Matt Dickinson was struggling to breathe when his mother rushed him to an Adelaide hospital earlier this month.

The 20-year-old has cystic fibrosis, which causes mucus to form in the lungs, and an infection had made him fall dangerously ill.

"His body was producing so much mucus that he couldn't keep up with clearing it," his mother, Cassandra Day, said.

"He told me, 'I feel like I'm drowning' and I've never heard him say that before.

"He said, 'I feel like I have water in my lungs. I can't breathe. It's heavy. I'm struggling.' It was terrifying."

Adding to the family's distress, COVID-19 protocols meant Ms Day could not be with her son, who also has autism, while he was in hospital.

"It was petrifying because he was so sick," Ms Day said.

"And then the phone calls that I was getting from the doctors were of significant concernthey were sharing how concerned they were and I felt absolutely powerless.

"I had no idea how my boy was, I didn't know if he was going to come back from it."

Matt ended up spending weeks in hospital hooked up to IV antibiotics - including his 20th birthday - without his family.

Lengthy hospital stays are familiar ground for many of the 3500 Australians living with cystic fibrosis, the country's most common genetic disease.

The condition has no cure and recurrent infections cause irreversible damage, with lung failure the most common cause of death.

Around half of those with cystic fibrosis currently pass away by the age of 30.

Ms Day said watching her son's condition decline was even more devastating knowing there was a medication that could give him his life back.

While the drug Trikafta is available to patients in Australia, it is incredibly expensive, costing more than $300,000 per year.

Ms Day is one of many campaigning for Trikafta to be added to the Pharmaceutical Benefits Scheme (PBS) for everyone with cystic fibrosis.

A petition with 35,000 signatures calling for Trikafta to be subsidised under the PBS was presented to Federal Parliament in November by Labor MP and paediatrician Mike Freelander.

"Trikafta is a life-saving and life-changing treatment for people for people with cystic fibrosis," Mr Freelander told parliament at the time.

"We have seen people taken off the heart/lung transplant list. We're seeing people's lives saved."

In July last year, the Pharmaceutical Benefits Advisory Committee (PBAC) recommended Trikafta be listed on the PBS, but only for those aged over 12 and with a specific mutation of cystic fibrosis.

Genevieve Handley, who started the parliamentary petition, said the decision would only cover about 20 percent of the 2200 people waiting to access the medication.

Vertex Pharmaceuticals, which manufactures the drug, is also yet to proceed with the PBAC recommendation.

Listing Trikafta on the PBS was also discussed at a PBAC meeting in December, with the independent advisory panel's decision expected to be made public tomorrow.

Ms Handley said people's lives were depending on Friday's PBAC outcome and Vertex Pharmaceuticals agreeing to list Trikafta.

"More than 25 countries have provided access and reached agreements before Australia," Ms Handley said.

"There are 2200 Australians who need Trikafta now.

"The CF community desperately needs a 'yes' from the PBAC, followed by access soon afterwards."

Ms Handley, who has cystic fibrosis, said Trikafta had been a gamechanger for her after she was approved for Vertex's limited compassionate access scheme, three days before she was due to meet with a lung transplant team.

"It's improved my lung function from 34 percent to 55 percent," she said.

"My lung bleeds that had continued for months have stopped and I no longer need to see the transplant team.

"I can breathe without hearing crackles or wheezing, rarely cough and can walk along the beach and talk at the same time."

Ms Day said hearing positive stories about other people who were able to access Trikafta gave her hope that Matt would soon be able to benefit.

"He has been so sick but having access to this medication would basically give him his life back and help him be able to realise that that he can do more than just be the sick kid," she said.

"I think our kids deserve a future."

9news.com.au has contacted the Department of Health for comment.

A spokesperson for Vertex Pharmaceuticals said: "Vertex has always maintained its focus to ensure equitable access to Trikafta for all eligible Cystic Fibrosis patients in Australia."

"Trikafta has been available internationally for up to two years. There are now more than 25 countries, including Canada, the UK, France, Germany, and the Netherlands, that have recognised the value Trikafta brings to the CF Community and funded the therapy for all eligible patients. Australian patients deserve the same."

A spokesperson for the Federal Health Department said in September that Vertex had still not provided a formal proposal for the drug to be reconsidered and listed for the broader cystic fibrosis population.

The spokesperson for Vertex said the proposal was submitted at the December PBAC meeting.

Contact reporter Emily McPherson at emcpherson@nine.com.au

Original post:

'I feel like I'm drowning': Thousands with cystic fibrosis wait to hear if 'life-saving' drug will be listed on PBS - 9News

Malaga CF has appointed a new coach today, Thursday, January 27. The announcement was made that 55-year-old Natxo Gonzalez from Vitoria-Gasteiz, in the Basque Country, will replace Jose Alberto Lopez, who was fired last Monday 24.

After several days of meetings and discussions, Gonzalez was deemed to be the most suitable, seemingly prepared to work within the tight financial restraints that the Costa del Sol club is under.

He has stepped into the role at a time when blue and whites are going through a poor spell of form. This was all too evident when they lost 5-0 to UD Ibiza last Saturday, which clearly led to the sacking of Lopez.

Gonzalez had coached top-flight teams in Bolivia and Portugal. He led Deportivo Alaves into Segunda B in 2013, and managed at Reus, Zaragoza and Deportivo in Sugunda B. In June 2018, he took control of Deportivo La Corua, which ended some 10 months later.

The problem facing Malaga in their search for a high-profile coach, such as Diego Martinez, or Pablo Machin, is that sadly they are basically unattainable at a sporting and economic level.

Pep Marti seemed to be a good fit, but allegedly his financial expectations were too high, and Malagas offer was quite humble. It has to be remembered that the Malacitano club fights at a financial disadvantage, and has a very tight league salary with which to entice a top coach.

This applies to the signing of players as well, with only a small budget available to try and attract the quality necessary to make the jump back up into LaLiga. Espanyol winger Alvaro Vadillo arrived on loan earlier this month, adding some much-needed variety out wide, as reported by malagahoy.es.

For more info on football in Spain: https://www.euroweeklynews.com/news/spain-football/

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Thank you for reading, and dont forget to check The Euro Weekly News for all your up-to-date local and international news stories, and remember, you can also follow us onFacebookandInstagram.

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Malaga CF announce the appointment of a new head coach - Euro Weekly News

The latest market intelligence studies oncarbon fiber (CF) and carbon fiber reinforced plastics (CFRP)are both primary and secondary studies to provide insights into predictive models, opportunities, and competitive environments forcarbon fiber (CF). It depends on the statistics obtained from.Carbon Fiber Reinforced Plastics (CFRP) Market Expected Period 2021-2028.

Importantly, this study leverages key data on niche segments, market share, size, and growth rates to reach out to business owners, field marketing executives, and stakeholders to other companies doing business in the same industry. Its about providing competitiveness.Well dig deeper into customer-focused aspects such as consumer power, changing customer preferences, and consumption patterns to learn more about trendy business processes and product utilization during forecast periods.

Request a sample copy of the carbon fiber (CF) and carbon fiber reinforced plastics (CFRP) market at the following URL:

Major manufacturers covered by this report:Solvay, Hexcel Corporation, Gurit, TORAY INDUSTRIES, INC, TEIJIN LIMITED., SGL Group, Mitsubishi Chemical Holding Corporation, Formosa Plastics Corporation, HYOSUNG., Koninklijke Ten Cate bv, Aeron Composite Pvt.Ltd., Sigmatex., Nippon Graphite Fiber Corporation, Crossby Composites, DowAksa, UMATEX, ROSATOM State Corporation, Kemrock Industries and Exports Limited, KUREHA CORPORATION, Plasma Carbon Composites, ELG Carbon Fiber Ltd.

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The research on theCarbon Fibers (CF) and Carbon Fiber Reinforced Plastics (CFRP) marketconcentrates on extracting valuable data on swelling investment pockets, significant growth opportunities, and major market vendors to help understand business owners what their competitors are doing best to stay ahead in the competition. The research also segments the Carbon Fibers (CF) and Carbon Fiber Reinforced Plastics (CFRP) market on the basis of end user, product type, application, and demography for the forecast period 20212028.Detailed analysis of critical aspects such as impacting factors and competitive landscape are showcased with the help of vital resources, which include charts, tables, and infographics.

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For more clarity on the real potential of the Carbon Fibers (CF) and Carbon Fiber Reinforced Plastics (CFRP) market for the forecast period 20212027, the study provides vital intelligence on major opportunities, threats, and challenges posed by the industry. Additionally, a strong emphasis is laid on the weaknesses and strengths of a few prominent players operating in the same market. Quantitative assessment of the recent momentum brought about by events such as collaborations, acquisition and mergers, product launches and technology innovation empower product owners, as well as marketing professionals and business analysts make a profitable decision to reduce cost and increase their customer base.

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The Carbon Fiber (CF) and Carbon Fiber Reinforced Plastics (CFRP) markets will generate significant profits in the future | Solvay, Hexcel...

What happened

Shares of the special purpose acquisition company (SPAC) CF Acquisition Corp VI (NASDAQ:CFVI) rose as much as 10.5% today before ending the day up nearly 5%. While it may not be the exact reason, the company that CF Acquisition Corp intends to merge with and take public, Rumble, announced today that it plans to invest in the payments company Parallel Economy.

Rumble is a video publishing platform that claims to have fewer restrictions than other more-traditional publishing platforms like YouTube. Rumble has gotten popular among publishers worried about having their content banned from traditional publishing platforms. The company said last year that it had reached 36 million average active monthly users.

Image source: Getty Images.

Launched last year, Parallel Economy is a payment processor that has flat pricing, no monthly surcharges, and next-day funding. The company claims to be "censor resistant."

Rumble CEO Chris Pavlovski said in a statement:

Our mission is to eliminate every chokepoint and backdoor to cancel companies like Rumble and our creators. This investment in a digital payment processing system is another example of Rumble's commitment to put creators first. With Parallel Economy, creators won't have to worry about arbitrary cancellation. Giving creators financial security is a critical part of Rumble's mission to protect a free and open Internet.

Rumble does seem to have built a sizable audience. Additionally, platforms like Rumble and Truth Social, whose parent company is being taken public by the SPAC Digital World Acquisition Corp, are clearly seen as two disruptors in the world of traditional social media and tech.

It's a bit early to know if platforms like this are here to stay, and ultimately I think more information and performance history are needed to prove these concepts out.

This article represents the opinion of the writer, who may disagree with the official recommendation position of a Motley Fool premium advisory service. Were motley! Questioning an investing thesis -- even one of our own -- helps us all think critically about investing and make decisions that help us become smarter, happier, and richer.

Link:

Why Shares of CF Acquisition Corp VI Rose Today - Motley Fool

QUEBEC, Jan. 18, 2022 /PRNewswire/ - Feldan Therapeutics, a biopharmaceutical company that develops therapeutics based on its proprietary intracellular delivery platform, announced today it was awarded funding by the Cystic Fibrosis Foundation to pursue the development of the Feldan Shuttle technology as a pulmonary delivery agent for cystic fibrosis (CF).

The award is part of the Cystic Fibrosis Foundation's US$500 million Path to a Cure initiative to accelerate treatments for the underlying cause of CF and to cure every person with the disease.

Relentless efforts in seeking therapies to minimize symptoms or to cure CF are often hindered by the barriers imposed by the specialized cells, secreted host defense factors and mucociliary transport system lining the airway of CF patients. Therefore, developing an efficient pulmonary delivery agent is instrumental in the advancement of treatments for CF. The Feldan Shuttle technology offers great promise as it has been demonstrated to enable delivery of various types of functional therapeutic cargos into cultured human airway epithelial cells, a breakthrough in the pulmonary field.

"We are very enthusiastic about this support from the Cystic Fibrosis Foundation," said Franois-Thomas Michaud, co-founder and Chief Executive Officer of Feldan. "We are looking forward to advancing safe delivery of chemical and biological cargos to CF airway epithelia through this grant and taking an active role in offering new therapeutic avenues to CF patients."

About Feldan Therapeutics:

Feldan is a biopharmaceutical company that specializes in the development of treatments based on the intracellular delivery of therapeutics. Feldan designed a proprietary, peptide-based technology that enables safe and efficient delivery of various compounds inside cells. The Shuttle technology unlocks the development of a new generation of therapiesby giving access to intracellular components beyond the reach of existing drugs. Feldan's pipeline focuses on diseases affecting lungs and skin.

For more information: http://www.feldan.com

SOURCE Feldan Therapeutics

Continued here:

Feldan Therapeutics Awarded Funding by Cystic Fibrosis Foundation - PRNewswire

TDT | Manama

The Daily Tribunewww.newsofbahrain.com

His Highness Shaikh Nasser bin Hamad Al Khalifa, Representative of His Majesty the King for Humanitarian Works and Youth Affairs, asserted the importance of the training camp carried out by the Spanish team Cordoba CF in Bahrain, adding that it was influential in promoting the Kingdoms remarkable achievements in various sectors, including the sports field.

In addition, His Highness indicated that the gains of this visit will raise the technical and experience levels of the players of the national clubs, which will significantly benefit the Bahraini national team.

On the occasion of the end of the Spanish teams training camp, HH Shaikh Nasser bin Hamad Al Khalifa stated The visit came to promote Bahrain and its brilliant successes during the prosperous era of His Majesty King Hamad bin Isa Al Khalifa, the King of Bahrain.

Cordobas training camp highlights the renaissance of Bahraini sports and Bahrains high capabilities that have placed it at the forefront of the host countries for various sports tournaments and teams.

The visit of the Spanish team comes in line with Bahrain Economic Vision 2030, which is keen on promoting Bahrainthrough sports as an ideal way to convey the messages of Bahrain in various fields.

And it confirms Bahrains position as the capital of youth and sports that make it shine in the global spotlight, HH Shaikh Nasser bin Hamad continued.

His Highness noted that the presence of the Spanish team in Bahrainwill have positive repercussions on Bahraini football.

The friendly games that brought Cordoba CF together with Al Khaldiya Sports Club and Riffa Sports Club provided the teams with a broad space to gain expertise and technical skills, which was achieved thanks to the interactions with the skilled players of Cordoba CF.

The benefits of these games will be reflected in the performance of the two teams and the national team, which includes an elite group of players from Al Khaldiya and Riffa.

HH Shaikh Nasser said, Cordobas training camp has opened wide areas of cooperation with the Bahraini clubs and the Bahrain Football Association, which we hope will culminate in the development of Bahraini football and serve us in discovering more sports talents.

This is because the Spanish team possesses high levels of technical experience and skills in management and the discovery of talents in football.

Cordoba CF played two friendly matches in Bahrain, winning the first match against Al Khaldiya by 3-1 goals and drawing 1-1 in the second one with Riffa.

The two matches were held at the Bahrain National Stadium.

The Spanish team left Bahrain after the conclusion of its training camp, which witnessed various activities and numerous visits to tourist and heritage sites inBahrain.

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Cordoba CF training camp to benefit Bahraini national team: HH Shaikh Nasser | THE DAILY TRIBUNE | KINGDOM OF BAHRAIN - News of Bahrain- DT News