Interspecies Hybrids Play a Vital Role in Evolution – Quanta Magazine

Controversies like this one underscore the possibility that the bad reputation of naturally occurring hybrids is not entirely justified. Historically, hybrids have often been associated with the sterile or unfit offspring of maladaptive crossings (such as the mule, born of a female horse and a male donkey). Naturalists have traditionally viewed hybridization in the wild as a kind of irrelevant, mostly rare, dead-end fluke. If hybrids arent viable or fertile or common, how could they have much influence on evolution? But as genomic studies provide new insights into how species evolve, biologists are now seeing that, surprisingly often, hybrids play a vital role in fortifying species and helping them take on useful genes from close relatives.

In short, maladaptive pairings dont tell the full story of interbreeding. The genetic transfer that takes place between organisms while their lineages are diverging has a hand in the emergence of adaptive traits and in the creation of new species altogether. According to Arnold, not only is it common for newly emerging species to reacquire genes through hybrid populations, but its probably the most common way evolution proceeds, whether youre talking about viruses, plants, bacteria or animals.

Most recently, signatures of hybridization have turned up in studies on the evolution of the jaguar. In a paper published last month in Science Advances, a team of researchers from institutions spanning seven countries examined the genomes of the five members of the Panthera genus, often called the big cats: lions, leopards, tigers, jaguars and snow leopards. The scientists sequenced the genomes of the jaguar and leopard for the first time and compared them with the already existing genomes for the other three species, finding more than 13,000 genes that were shared across all five. This information helped them construct a phylogenetic tree (in essence, a family tree for species) to describe how the different animals diverged from a common ancestor approximately 4.6 million years ago.

Some of these adaptations, however, may not have originated in the jaguar lineage at all. Eiziriks team found evidence of many crossings between the different Panthera species. In one case, two genes found in the jaguar pointed to a past hybridization with the lion, which would have occurred after their phylogenetic paths had forked. Both genes turned out to be involved in optic nerve formation; Eizirik speculated that the genes encoded an improvement in vision the jaguars needed or could exploit. For whatever reasons, natural selection favored the lions genes, which took the place of those the jaguar originally had for that trait.

Such hybridization illustrates why the Eizirik groups delineation of the Panthera evolutionary tree is so noteworthy. The bottom line is that this has all become more complex, Eizirik said. Species eventually do become separated, but its not as immediate as people would frequently say. He added, The genomes we studied reflected this mosaic of histories.

Although supporting data as detailed and as thoroughly analyzed as Eiziriks is rare, the underlying idea that hybridization contributes to species development is by no means new. Biologists have known since the 1930s that hybridization occurs frequently in plants (its documented in about 25 percent of flowering plant species in the U.K. alone) and plays an important role in their evolution. In fact, it was a pair of botanists who, in 1938, coined the phrase introgressive hybridization, or introgression, to describe the pattern of hybridization and gene flow they saw in their studies. Imagine members of two species lets call them A and B that cross to produce 50-50 hybrid offspring with equal shares of genes from each parent. Then picture those hybrids crossing back to breed with members of species A, and assume that their offspring do the same. Many generations later, nature is left with organisms from species A whose genomes have retained a few genes from species B. Studies have demonstrated that this process could yield entirely new plant species as well.

But animal species seemed more discrete, at least for a while. Most zoologists supported the biological species concept proposed in 1942 by the legendary biologist Ernst Mayr, who was one of the architects of the modern synthesis, the version of evolution theory that combined Darwins natural selection with the science of genetics. Mayrs biological species concept was based on reproductive isolation: A species was defined as a population that could not or did not breed with other populations. Even when exceptions to that rule started to emerge in the 1970s, many biologists considered hybridization to be too rare to be important in animals. We had a blinkered attitude, said James Mallet, an evolutionary biologist at Harvard University. Today, he added, saying that such hybridizations dont affect reconstructions of evolutionary history or that this wasnt useful in adaptive evolution thats no longer tenable.

This is especially true now that computational and genomic tools prove just how prolific introgression is even in our own species. Since 2009, studies have revealed that approximately 50,000 to 60,000 years ago, some modern humans spreading out of Africa interbred with Neanderthals; they later did so with another ancestral human group, the Denisovans, as well. The children in both cases went on to mate with other modern humans, passing the genes they acquired down to us. At present, researchers estimate that some populations have inherited 1 to 2 percent of their DNA from Neanderthals, and up to 6 percent of it from Denisovans fractions that amount to hundreds of genes.

In 2012, Mallet and his colleagues showed a large amount of gene flow between two hybridizing species of Heliconius butterfly. The following year, they determined that approximately 40 percent of the genes in one species had come from the other. Mallets team is now working with another pair of butterfly species that exchange even more of their genes: something like 98 percent, he said. Only the remaining 2 percent of the genome carries the information that separates the species and reflects their true evolutionary trajectory. A similar blurring of species lines has already been found in malaria-carrying mosquitoes of the Anopheles genus.

Other types of organisms, from fish and birds to wolves and sheep, experience their share of introgression, too. The boundaries between species are now known to be less rigid than previously thought, said Peter Grant, an evolutionary biologist at Princeton University who, along with his fellow Princeton biologist (and wife) Rosemary Grant, has been studying the evolution of Galpagos finches for decades. Phylogenetic reconstructions depict treelike patterns as if there is a clear barrier between species that arises instantaneously and is never breached. This may be misleading.

Arnold concurred. Its a web of life, he said, rather than a simple bifurcating tree of life. That also means its more necessary than ever before to examine the entire genome, and not just selected genes, to understand a species evolutionary relationships and generate the correct phylogeny. And even that might not be enough. It may well be, Mallet said, that some actual evolutionary patterns are still completely irrecoverable.

Genomic studies cant create a complete picture of the introgressive movements of genes. Whenever one species inherits genes from another, the outcome can be either deleterious, neutral or adaptive. Natural selection tends to weed out the first, although some of the genes we have inherited from Neanderthals, for example, may be involved in disorders such as diabetes, obesity or depression. Neutral introgressed regions drift, so its possible for them to remain in the genome for very long periods of time without having an observable effect.

But its the beneficial introgressions that particularly fascinate researchers. Take the Neanderthal and Denisovan DNA again: Those genes have allowed people to adapt to the harsh environs of places like the Tibetan plateau, protecting them against the harmful effects of high altitudes and low oxygen saturation, which in nonlocals can cause stroke, miscarriage and other health risks. Variants from interbreeding with archaic humans have also conferred immunity to certain infections and made skin and hair pigmentation more suitable for Eurasian climes.

Mallets butterflies, too, reflect evidence of adaptive hybridization, particularly with traits involved in mimicry and predator avoidance. Researchers had observed that although most Heliconius species had highly divergent wing coloration and patterning, some bore a striking resemblance to one another. The researchers believed that these species had independently converged on these traits, but it turns out thats only partially correct. Mallet and others have found that introgression was also responsible. The same goes for Galpagos finches: Pieces of their genomes that control for features including beak size and shape were shared through hybridization. Once again, parallel evolution cant explain everything.

For these effects to occur, the rate of hybridization can be and most likely is very small. For Mallets almost entirely hybridized butterflies, the occasional trickle of one hybrid mating every 1,000 normal matings is sufficient to completely homogenize genes between the species, he said. Thats pretty exciting.

As these patterns of introgression have become more and more predominant in the scientific literature, researchers have set out to uncover their evolutionary consequences. These go beyond the fact that speciation tends to be a much more gradual process than its often made out to be. Diversification, adaptation and adaptive evolution really do seem to be driven quite often by genes moving around, Arnold said.

The research done by Eizirik and his team makes a compelling case for this. Around the time when the gene introgressions they analyzed occurred, the populations of all five Panthera species are estimated to have declined, likely due to climate changes. The smaller a population is, the greater the probability that a harmful mutation will get affixed to its genome. Perhaps the gene flow found between the different species, then, rescued them from extinction, providing adaptive mutations and patching deleterious ones. This kind of infusion of genetic mutations is so large that it can cause really rapid evolution, Arnold said.

And the process doesnt end with speeding up evolution in a single species. Adaptive introgression can in turn contribute significantly to adaptive radiation, a process by which one species rapidly diversifies into a large variety of types, which then form new lineages that continue to adapt independently. The textbook case can be found in the great lakes of East Africa, which are home to hundreds upon hundreds of cichlid species, a type of fish that diversified in explosive bursts (on the evolutionary timescale) from common ancestors, largely in response to climatic and tectonic shifts in their environment. Today, cichlids vary widely in form, behavior and ecology thanks in large part to introgressive hybridization.

Biologists will need many more years to understand the full importance of hybridization to evolution. For example, Arnold wants to see further investigations like the ones that have been done on the finches in the Galpagos and the wolves of Yellowstone National Park: behavioral, metabolic and other analyses that will reveal how much of introgression is adaptive and how much is deleterious or neutral as well as whether adaptive introgression affects only particular kinds of genes, or if it acts in a more widespread manner.

Unfortunately, for conservationists and others challenged with managing the diversity of imperiled species, the absence of satisfactory answers poses more immediate problems. They must often weigh the value of protecting wild hybrid populations against the harm hybrids can do to established species, including the ones from which they emerged.

A case in point: In the 1950s, a pair of California bait dealers from the Salinas Valley, seeking to expand their business, hopped into a pickup truck and took off to central Texas and New Mexico. They brought back barred tiger salamanders, which could grow to more than double the size of Californias native tiger salamander. The new species quickly proved to be good for the local fishermen but bad for the local ecosystem: The introduced salamanders mated with the natives, creating a hybrid breed that could outcompete its parent species. Soon the California tiger salamander found itself in danger of being wiped out entirely, and it remains a threatened species today.

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Interspecies Hybrids Play a Vital Role in Evolution - Quanta Magazine