Euthanasia (Guideline) | Vertebrate Animal Research

Guidelines: The IACUC has provided a set of guidance documents (Policies, Guidelines, and Informational Sheets) for use when planning animal procedures at the University of Iowa. An exception to a Guideline must be described and justified in the Animal Protocol and approved during the normal review process.

Purpose: The purpose of these guidelines is to describe acceptable methods for the euthanasia of animals used in teaching, research and testing at the University of Iowa. All animal euthanasia must be performed by appropriately trained personnel approved on the Animal Protocol.

Performance of Euthanasia

All animal euthanasia must be performed by appropriately trained personnel approved on the Animal Protocol. All euthanasia procedures must be continuously monitored by the person(s) performing the procedure, until confirmation of euthanasia is complete.

Confirmation of EuthanasiaAny animal euthanized on a University of Iowa Animal Protocol requires a method of confirmation of euthanasia. Some acceptable methods of confirmation are described below.

Common Acceptable Methods of EuthanasiaListed below are some commonly used and accepted methods of euthanasia for different species. This list is not inclusive. Please see the AVMA Guidelines on Euthanasia for further information.

Rodents weighing > 500grams

Rodents weighing <500 grams

Rodent CO2 Euthanasia

Isoflurane Euthanasia

Confirm Euthanasia as described previously in this document

Xenopus

Zebrafish

Birds

Non-Rodent Mammals

Euthanasia by Perfusion

Physical Methods of Euthanasia On Un-Anthesthetized Animals > 10 days old

References

"AVMA Guidelines for the Euthanasia of Animals: 2013 Edition" AVMA.org., 2013.

Last Reviewed by the IACUC 1/08/2020

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Euthanasia (Guideline) | Vertebrate Animal Research