Cloning Vector and its Characteristics | Chemistry Learning

A cloning vector is a DNA molecule in which foreign DNA can be inserted or integrated and which is further capable of replicating within host cell to produce multiple clones of recombinant DNA. Such a vector is called Cloning Vector. Plasmids and phages are the vectors used for cloning purposes, particularly in prokaryotes (bacteria).

A cloning vector is a DNA molecule in which foreign DNA can be inserted or integrated and which is further capable of replicating within host cell to produce multiple clones of recombinant DNA. Such a vector is called Cloning Vector. Plasmids and phages are the vectors used for cloning purposes, particularly in prokaryotes (bacteria).

This process marks autonomous replication in vector. ORI is a specific sequence of nucleotide in DNA from where replication starts. When foreign DNA is linked to this sequence then along with vector replication, foreign (desirable) DNA also starts replicating within host cell.

Besides ORI, a cloning vector should have selectable marker gene. This gene permits the selection of host cells which bear recombinant DNA (called transformants) from those which do not bear rDNA (non-transformants).

It should have restriction sites, to allow cleavage of specific sequence by specific Restriction Endonuclease. Restriction sites in E.coli cloning vector pBR322 include HindIII , EcoRI , BamHI , SalI, PvuI, PstI, ClaI etc.

Structure of E.Coli plasmid cloning vector pBR322

Pic Credit : Link

Credit : Link

Mar 11, 2009admin

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Cloning Vector and its Characteristics | Chemistry Learning