The eyes are a gateway to evolution of daddy longlegs at least. – University of Wisconsin-Madison

A male emerald harvestman (Iporangaia pustulosa) guarding its eggs in the Atlantic rain forest of Brazil. Studying the embryos this species revealed that vestigial eyes may be widespread in harvestmen. Photo: John Uribe

While some people may first associate daddy longlegs with, well, their long legs, researchers Guilherme Gainett and Prashant Sharma have been especially focused on the arachnids eyes. In their paper published last week in the journal Current Biology, the researchers found that a living species of daddy longlegs has two additional sets of underdeveloped eyes as embryos, implying that the species diversified earlier in the evolutionary tree than scientists believed.

The clue to this unexpected discovery lay hidden under the microscope, beside the developing legs of a daddy longlegs.

I couldnt believe my eyes. It was one of those little moments that happens in science when you see something that no one has ever seen and get really excited, recalls Gainett, who is now a post-doctoral researcher at Boston Childrens Hospital and Harvard Medical School.

What Gainett was seeing were opsins, visual proteins that are important for the formation of visual organs and the mechanics of sight. Finding these opsins clustered on the embryos indicated to the researchers that they were likely vestigial eyes, structural remnants that, at another point in evolution, would have developed into two additional sets of lens-bearing, functional eyes.

Under a microscope, the head of a daddy longlegs glows magenta where its modern-day eyes form. Opsins shown in green indicate that two additional sets of vestigial eyes existed in an earlier stage of the animals evolution. Photo: Guilherme Gainett

One set was lateral, located on the side of the head beside the frontmost pair of legs, while the other was a median set, located in front of eyes that appear today on the front of the head. Gainett and Sharma also found evidence that these organs have remained connected to the visual processing part of the brain.

Until now, present-day daddy longlegs were believed to have only one set of median eyes, positioned at the top of the head. While a fossilized specimen of a more ancient version of the arachnid found in 2014 had an additional set of lateral eyes on the side of the head, the new study is the first to show evidence of more than one eye set in a living daddy longlegs species.

Vestigial organs have been of interest to scientists since Charles Darwin began discussing his ideas of evolution.

They leave these footprints behind in the crime scene of what happened in the transition from one morphology to the other in different groups, Gainett says.

He explains that vestigial organs are important because they can link the ancient and modern traits of species, allowing us to track the evolutionary process as a species gains or as with this case loses features over time. Interestingly, the position of the vestigial eyes found in living daddy longlegs are similar to what was reported in the fossilized specimen.

Different eyes have different functions, Gainett explained. Median eyes usually have better visual acuity, so theyre better at forming crisp images in some arachnids. Lateral eyes are helpful for detecting movement and light in general, not so much for producing clear images, making them helpful in low-light conditions.

But since the lateral eyes Gainett found on the daddy longlegs embryos dont have lenses, they arent processing visual information into images in the same way that fully developed eyes do. Instead, Gainett and Sharma believe these eyes are picking up on more general information to help the arachnid tell the difference between light and dark.

Once they discovered the vestigial opsins, Gainett and Sharma began to wonder why and how the daddy longlegs ancestor lost two pairs of eyes. Its difficult to know what environmental conditions could have played a roll, so instead they looked to the genetic and developmental conditions that give rise to different kinds of eyes.

Gainett and Sharma studied a group of genes that code for the development of arachnid eyes.

Guilherme Gainett

Some of these genes are expressed in both types of eyes, so theyre necessary for all eyes, for median eyes and lateral eyes. But some of these genes are more restricted to one type or another, says Gainett.

By experimenting with knocking down genes expressions, they were able to provide evidence that the vestigial organs were likely eyes. Gainett explains knocking down genes as a temporary way to dim down their expression, rather than permanently switching them off entirely.

To dim the expression of genes responsible for eye development, the researchers used a process called RNA interference. It essentially tricks a cell into thinking its own gene is a threat and, like a virus, should be chopped up so it cant be duplicated. When the expression of these genes was lowered in daddy longlegs embryos, the individuals did not develop eyes.

Each row in the image above illustrates the head of a single embryo at increasing developmental stages of eye formation. Magenta, yellow and green mark the expression of three different genes that code for vision. The first column is a composite to show all three genes at the same time. Photo: Guilherme Gainett

Drawing these genetic and developmental links between the vestigial eyes of a present-day daddy longlegs and the fossilized specimen gives an important look inside how evolution happens.

One of the goals of studying the genetics behind what we see in morphology is really understanding how evolution happens. What do you need to change in the underlying recipe of genes for the diversity of eyes to evolve? Gainett explains.

At Boston Childrens Hospital, Gainett hopes to continue his work on arthropod eye evolution by studying the genetics behind the transition from compound eyes, like those of flies, to camera-like eyes that we see in many spiders.

Funding for this research comes from the National Science Foundation, grant no. IOS-2016141.

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The eyes are a gateway to evolution of daddy longlegs at least. - University of Wisconsin-Madison