In the Shadow of Rome: Ancient DNA Recasts Balkan History – SciTechDaily

A comprehensive DNA analysis of the Balkan population from 1 to 1000 CE challenges the assumed Roman influence, revealing no Italian genetic traits. Instead, the study uncovers significant ancestry from Western Anatolia, Europe, and the Pontic-Kazakh Steppe, with a notable Slavic migration shaping modern Balkan genetics. Credit: SciTechDaily.com

Despite the Roman Empires extensive military and cultural influence on the nearby Balkan peninsula, a DNA analysis of individuals who lived in the region between 1 and 1000 CE found no genetic evidence of Iron Age Italian ancestry. Instead, a study published December 7 in the journal Cell revealed successive waves of migrations from Western Anatolia, central and northern Europe, and the Pontic-Kazakh Steppe during the Empires reign.

From the 7th century CE onwards (coincident with the fall of the Western Roman Empire), large numbers of people emigrated from Eastern Europe, likely related to the arrival of Slavic-speaking populations, which resulted in present-day Balkan residents having 30%60% Slavic ancestry seen in present-day Balkan peoples.

Skull of an individual of East African ancestral origin found in Viminacium, with the oil lamp featuring an eagle found in his tomb. Credit: Miodrag (Mike) Grbic

We found this genetic signal of Slavic migration all across the Balkans, says senior author and paleogenomicist Carles Lalueza-Fox of the Institute of Evolutionary Biology (IBE:CSIC-Universitat Pompeu Fabra) and Museu de Cincies Naturals de Barcelona. This could have important social and political implications given that the Balkans has had a long history of conflict associated with their perceived identities.

Most ancient DNA studies focus on pre-historybefore the written recordbut ancient DNA methods can also provide insight into more recent historical periods, especially when used in combination with historical and archeological information.

Ancient DNA can give a lot of insight into historical periods, especially for regions where historical sources are scarce or when we dont know whether sources are biased or not, says first author and population geneticist Iigo Olalde of the University of the Basque Country (UPV/EHU). For example, most historical sources from the Balkans are written from the side of the Romans because the Slavic people didnt write at that time.

This photograph shows the Mausoleum of Viminacium. Credit: Carles Lalueza-Foz

Previous studies have investigated the ancestry of people who lived in Italy and England during and after the fall of the Roman Empire, but little is known about demography and ancestry of the Balkans during this time. This region was one of the distant frontiers of the Roman Empire, which makes it interesting to study because this is clearly a place where you would expect people to come in contact with people from outside the Empire, so you can test things such as globalization, says Olalde.

To explore the population history of the Balkans and examine the influence of the rise and fall of the Roman empire, the researchers extracted DNA from 136 ancient individuals excavated from 20 different sites across the Balkansdefined as the region bounded by the Adriatic, Central Mediterranean, and Aegean Seas and the Middle and Lower Danube and Sava Rivers. These sites included large Roman cities, military fortresses, and small rural towns. The team focused on three periods: during the expansion and height of the Roman Empire (1250 CE), during the late Imperial period (circa 250550 CE), and following the Western Empires collapse (5501000 CE).

To provide cultural and historical context for the genetic data, the team collaborated with local archeologists and historians. For each grave, they documented burial type, as well as any objects buried alongside the individuals, such as coins, jewelry, pottery, tools, and weapons. The researchers also used radiocarbon dating to verify the age of 38 of the ancient individuals, which generated isotopic data that provide a window into those individuals diets.

This photograph shows a Roman aqueduct that supplied water to Viminacium, a large Roman city. Credit: Carles Lalueza-Foz

The researchers were surprised to find no evidence of Italian Iron Age ancestry in the Balkan populations during the height of the Roman Empire. Instead, they showed that there was an influx of people from Western Anatolia, another part of the Roman Empire, during that period. They also found evidence of individual migrations into the Balkans from both within and outside the Roman Empire. Notably, a 16-year-old male who was excavated from a necropolis in a large Roman city was of 100% East African ancestry. The individual was buried with an oil lamp depicting Jupiter-related eagle iconography, but isotopic analysis of his teeth indicated that he had consumed marine protein sources during his childhood and therefore had likely grown up in a distant location.

This was the only full Eastern African individual that we analyzed, and he was also a clear outlier with respect to the diet compared to the rest of the individuals buried in the same necropolis, which tells us that this individual clearly grew up outside the borders of the Roman Empire, says Lalueza-Fox.

During the late Imperial period, between 250 and 550 CE, the researchers detected migrants with mixed ancestry from Northern Europe and the Pontic-Kazakh steppe. We found that those two ancestriescentral/northern European and Sarmatian-Scythian tended to come together, which suggests that these are likely to have been multi-ethnic confederations of moving people, says senior author and population geneticist David Reich of Harvard University.

However, these sources of ancestry disappeared after 700 CE. From 600 CE, shortly after the fall of the Western Roman Empire, there was a major influx of individuals from Eastern Europe. After 700 CE, individuals in the Balkans had very similar ancestral composition to present-day groups in the region, suggesting that these migrations resulted in the last large demographic shift in the area. These migrations coincide with recorded Slavic migrations, but the DNA analysis provides insight into the scale of these migrations that is impossible to glean from historical resources.

There have been debates about how impactful these migrations were and to what extent the spread of Slavic language was largely through cultural influences or movements of people, but our study shows that these migrations had a profound demographic effect, says Reich. More than half of the ancestry of most peoples in the Balkans today comes from the Slavic migrations, with around a third Slavic ancestry even in countries like Greece where no Slavic languages are spoken today.

The team are already planning what they call version two of the study, which will take advantage of improvements in ancient DNA technologies. We are now able to sequence hundreds of individuals from the same site, so we can go to another level of resolution and start to understand more about the social interactions and kinship between the different individuals, says Olalde.

Reference: A genetic history of the Balkans from Roman frontier to Slavic migrations by Iigo Olalde, Pablo Carrin, Ilija Miki, Nadin Rohland, Swapan Mallick, Iosif Lazaridis, Matthew Mah, Miomir Kora, Sneana Golubovi, Sofija Petkovi, Nataa Miladinovi-Radmilovi, Dragana Vulovi, Timka Alihodi, Abigail Ash, Miriam Baeta, Juraj Bartk, eljka Bedi, Maja Bili, Clive Bonsall, Maja Buni, Domagoj Buani, Mario Cari, Lea ataj, Mirna Cvetko, Ivan Drni, Anita Dugonji, Ana uki, Ksenija uki, Zdenk Farka, Pavol Jelnek, Marija Jovanovic, Iva Kai, Hrvoje Kalafati, Marijana Krmpoti, Sinia Krznar, Tino Lelekovi, Marian M. de Pancorbo, Vinka Matijevi, Branka Miloevi Zaki, Anna J. Osterholtz, Julianne M. Paige, Dinko Tresi Pavii, Zrinka Premui, Petra Raji ikanji, Anita Rapan Papea, Lujana Paraman, Mirjana Sanader, Ivana Radovanovi, Mirjana Roksandic, Alena efkov, Sofia Stefanovi, Maria Teschler-Nicola, Domagoj Tonini, Brina Zagorc, Kim Callan, Francesca Candilio, Olivia Cheronet, Daniel Fernandes, Aisling Kearns, Ann Marie Lawson, Kirsten Mandl, Anna Wagner, Fatma Zalzala, Anna Zettl, eljko Tomanovi, Duan Keckarevi, Mario Novak, Kyle Harper, Michael McCormick, Ron Pinhasi, Miodrag Grbi, Carles Lalueza-Fox and David Reich, 7 December 2023, Cell. DOI: 10.1016/j.cell.2023.10.018

This research was supported by the Spanish Ministry of Science of Innovation, la Caixa Foundation, the Natural Sciences and Engineering Research Council of Canada, the Ministry of Science and Education of the Republic of Croatia, the National Institutes of Health, the John Templeton Foundation, the Allen Discovery Center, the Paul G. Allen Family Foundation, and the Howard Hughes Medical Institute.

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In the Shadow of Rome: Ancient DNA Recasts Balkan History - SciTechDaily