Daily Archives: December 26, 2022

An offshore wind project being built with union labor could be exactly what energy workers need – Fortune

Posted: December 26, 2022 at 10:10 pm

An offshore wind project being built with union labor could be exactly what energy workers need  Fortune

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The nonprofit that works to boost offshore wind power in the US – Jefferson Public Radio

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The nonprofit that works to boost offshore wind power in the US  Jefferson Public Radio

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Genetic Engineering Principles of Biology

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Genetic engineering is the alteration of an organisms genotype using recombinant DNA technology to modify an organisms DNA to achieve desirable traits. The addition of foreign DNA in the form of recombinant DNA vectors generated by molecular cloning is the most common method of genetic engineering. The organism that receives the recombinant DNA is called a genetically modified organism (GMO). If the foreign DNA that is introduced comes from a different species, the host organism is called transgenic. Bacteria, plants, and animals have been genetically modified since the early 1970s for academic, medical, agricultural, and industrial purposes. In the US, GMOs such as Roundup-ready soybeans and borer-resistant corn are part of many common processed foods.

Although classical methods of studying the function of genes began with a given phenotype and determined the genetic basis of that phenotype, modern techniques allow researchers to start at the DNA sequence level and ask: What does this gene or DNA element do? This technique, called reverse genetics, has resulted in reversing the classic genetic methodology. This method would be similar to damaging a body part to determine its function. An insect that loses a wing cannot fly, which means that the function of the wing is flight. The classical genetic method would compare insects that cannot fly with insects that can fly, and observe that the non-flying insects have lost wings. Similarly, mutating or deleting genes provides researchers with clues about gene function. The methods used to disable gene function are collectively called gene targeting. Gene targeting is the use of recombinant DNA vectors to alter the expression of a particular gene, either by introducing mutations in a gene, or by eliminating the expression of a certain gene by deleting a part or all of the gene sequence from the genome of an organism.

The process of testing for suspected genetic defects before administering treatment is called genetic diagnosis by genetic testing. Depending on the inheritance patterns of a disease-causing gene, family members are advised to undergo genetic testing. For example, women diagnosed with breast cancer are usually advised to have a biopsy so that the medical team can determine the genetic basis of cancer development. Treatment plans are based on the findings of genetic tests that determine the type of cancer. If the cancer is caused by inherited gene mutations, other female relatives are also advised to undergo genetic testing and periodic screening for breast cancer. Genetic testing is also offered for fetuses (or embryos with in vitro fertilization) to determine the presence or absence of disease-causing genes in families with specific debilitating diseases.

Gene therapy is a genetic engineering technique used to cure disease. In its simplest form, it involves the introduction of a good gene at a random location in the genome to aid the cure of a disease that is caused by a mutated gene. The good gene is usually introduced into diseased cells as part of a vector transmitted by a virus that can infect the host cell and deliver the foreign DNA (Figure 1). More advanced forms of gene therapy try to correct the mutation at the original site in the genome, such as is the case with treatment of severe combined immunodeficiency (SCID).

Traditional vaccination strategies use weakened or inactive forms of microorganisms to mount the initial immune response. Modern techniques use the genes of microorganisms cloned into vectors to mass produce the desired antigen. The antigen is then introduced into the body to stimulate the primary immune response and trigger immune memory. Genes cloned from the influenza virus have been used to combat the constantly changing strains of this virus.

Antibiotics are a biotechnological product. They are naturally produced by microorganisms, such as fungi, to attain an advantage over bacterial populations. Antibiotics are produced on a large scale by cultivating and manipulating fungal cells.

Recombinant DNA technology was used to produce large-scale quantities of human insulin in E. coli as early as 1978. Previously, it was only possible to treat diabetes with pig insulin, which caused allergic reactions in humans because of differences in the gene product. Currently, the vast majority of diabetes suffers who inject insulin do so with insulin produced by bacteria.

Human growth hormone (HGH) is used to treat growth disorders in children. The HGH gene was cloned from a cDNA library and inserted into E. coli cells by cloning it into a bacterial vector. Bacterial HGH can be used in humans to reduce symptoms of various growth disorders.

Although several recombinant proteins used in medicine are successfully produced in bacteria, some proteins require a eukaryotic animal host for proper processing. For this reason, the desired genes are cloned and expressed in animals, such as sheep, goats, chickens, and mice. Animals that have been modified to express recombinant DNA are called transgenic animals. Several human proteins are expressed in the milk of transgenic sheep and goats, and some are expressed in the eggs of chickens. Mice have been used extensively for expressing and studying the effects of recombinant genes and mutations.

Manipulating the DNA of plants (i.e., creating GMOs) has helped to create desirable traits, such as disease resistance, herbicide and pesticide resistance, better nutritional value, and better shelf-life (Figure 3). Plants are the most important source of food for the human population. Farmers developed ways to select for plant varieties with desirable traits long before modern-day biotechnology practices were established.

Plants that have received recombinant DNA from other species are called transgenic plants. Because they are not natural, transgenic plants and other GMOs are closely monitored by government agencies to ensure that they are fit for human consumption and do not endanger other plant and animal life. Because foreign genes can spread to other species in the environment, extensive testing is required to ensure ecological stability. Staples like corn, potatoes, and tomatoes were the first crop plants to be genetically engineered.

Gene transfer occurs naturally between species in microbial populations. Many viruses that cause human diseases, such as cancer, act by incorporating their DNA into the human genome. In plants, tumors caused by the bacterium Agrobacterium tumefaciens occur by transfer of DNA from the bacterium to the plant. Although the tumors do not kill the plants, they make the plants stunted and more susceptible to harsh environmental conditions. Many plants, such as walnuts, grapes, nut trees, and beets, are affected by A. tumefaciens. The artificial introduction of DNA into plant cells is more challenging than in animal cells because of the thick plant cell wall.

Researchers used the natural transfer of DNA from Agrobacterium to a plant host to introduce DNA fragments of their choice into plant hosts. In nature, the disease-causing A. tumefaciens have a set of plasmids, called the Ti plasmids (tumor-inducing plasmids), that contain genes for the production of tumors in plants. DNA from the Ti plasmid integrates into the infected plant cells genome. Researchers manipulate the Ti plasmids to remove the tumor-causing genes and insert the desired DNA fragment for transfer into the plant genome. The Ti plasmids carry antibiotic resistance genes to aid selection and can be propagated in E. coli cells as well.

Bacillus thuringiensis (Bt) is a bacterium that produces protein crystals during sporulation that are toxic to many insect species that affect plants. Bt toxin has to be ingested by insects for the toxin to be activated. Insects that have eaten Bt toxin stop feeding on the plants within a few hours. After the toxin is activated in the intestines of the insects, death occurs within a couple of days. Modern biotechnology has allowed plants to encode their own crystal Bt toxin that acts against insects. The crystal toxin genes have been cloned from Bt and introduced into plants. Bt toxin has been found to be safe for the environment, non-toxic to humans and other mammals, and is approved for use by organic farmers as a natural insecticide.

The first GM crop to be introduced into the market was the Flavr Savr Tomato produced in 1994. Antisense RNA technology was used to slow down the process of softening and rotting caused by fungal infections, which led to increased shelf life of the GM tomatoes. Additional genetic modification improved the flavor of this tomato. The Flavr Savr tomato did not successfully stay in the market because of problems maintaining and shipping the crop.

Unless otherwise noted, images on this page are licensed under CC-BY 4.0 by OpenStax.

OpenStax, Biology. OpenStax CNX. May 27, 2016 http://cnx.org/contents/s8Hh0oOc@9.10:8CA_YwJq@3/Cloning-and-Genetic-Engineerin

Moen I, Jevne C, Kalland K-H, Chekenya M, Akslen LA, Sleire L, Enger P, Reed RK, Oyan AM, Stuhr LEB. 2012.Gene expression in tumor cells and stroma in dsRed 4T1 tumors in eGFP-expressing mice with and without enhanced oxygenation.BMC Cancer. 12:21. doi:10.1186/1471-2407-12-21 PDF

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Genetic Engineering Principles of Biology

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Engineering the Perfect Baby | MIT Technology Review

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Indeed, some people are adamant that germ-line engineering is being pushed ahead with false arguments. That is the view of Edward Lanphier, CEO of Sangamo Biosciences, a California biotechnology company that is using another gene-editing technique, called zinc fingers nucleases, to try to treat HIV in adults by altering their blood cells. Weve looked at [germ-line engineering] for a disease rationale, and there is none, he says. You can do it. But there really isnt a medical reason. People say, well, we dont want children born with this, or born with thatbut its a completely false argument and a slippery slope toward much more unacceptable uses.

Critics cite a host of fears. Children would be the subject of experiments. Parents would be influenced by genetic advertising from IVF clinics. Germ-line engineering would encourage the spread of allegedly superior traits. And it would affect people not yet born, without their being able to agree to it. The American Medical Association, for instance, holds that germ-line engineering shouldnt be done at this time because it affects the welfare of future generations and could cause unpredictable and irreversible results. But like a lot of official statements that forbid changing the genome, the AMAs, which was last updated in 1996, predates todays technology. A lot of people just agreed to these statements, says Greely. It wasnt hard to renounce something that you couldnt do.

The fear? A dystopia of superpeople and designer babies for those who can afford it.

Others predict that hard-to-oppose medical uses will be identified. A couple with several genetic diseases at once might not be able to find a suitable embryo. Treating infertility is another possibility. Some men dont produce any sperm, a condition called azoospermia. One cause is a genetic defect in which a region of about one million to six million DNA letters is missing from the Y chromosome. It might be possible to take a skin cell from such a man, turn it into a stem cell, repair the DNA, and then make sperm, says Werner Neuhausser, a young Austrian doctor who splits his time between the Boston IVF fertility-clinic network and Harvards Stem Cell Institute. That will change medicine forever, right? You could cure infertility, that is for sure, he says.

I spoke with Church several times by telephone over the last few months, and he told me whats driving everything is the incredible specificity of CRISPR. Although not all the details have been worked out, he thinks the technology could replace DNA letters essentially without side effects. He says this is what makes it tempting to use. Church says his laboratory is focused mostly on experiments in engineering animals. He added that his lab would not make or edit human embryos, calling such a step not our style.

What is Churchs style is human enhancement. And hes been making a broad case that CRISPR can do more than eliminate disease genes. It can lead to augmentation. At meetings, some involving groups of transhumanists interested in next steps for human evolution, Church likes to show a slide on which he lists naturally occurring variants of around 10 genes that, when people are born with them, confer extraordinary qualities or resistance to disease. One makes your bones so hard theyll break a surgical drill. Another drastically cuts the risk of heart attacks. And a variant of the gene for the amyloid precursor protein, or APP, was found by Icelandic researchers to protect against Alzheimers. People with it never get dementia and remain sharp into old age.

Church thinks CRISPR could be used to provide people with favorable versions of genes, making DNA edits that would act as vaccines against some of the most common diseases we face today. Although he told me anything edgy should be done only to adults who can consent, its obvious to him that the earlier such interventions occur, the better.

Church tends to dodge questions about genetically modified babies. The idea of improving the human species has always had enormously bad press, he wrote in the introduction to Regenesis, his 2012 book on synthetic biology, whose cover was a painting by Eustache Le Sueur of a bearded God creating the world. But thats ultimately what hes suggesting: enhancements in the form of protective genes. An argument will be made that the ultimate prevention is that the earlier you go, the better the prevention, he told an audience at MITs Media Lab last spring. I do think its the ultimate preventive, if we get to the point where its very inexpensive, extremely safe, and very predictable. Church, who has a less cautious side, proceeded to tell the audience that he thought changing genes is going to get to the point where its like you are doing the equivalent of cosmetic surgery.

Some thinkers have concluded that we should not pass up the chance to make improvements to our species. The human genome is not perfect, says John Harris, a bioethicist at Manchester University, in the U.K. Its ethically imperative to positively support this technology. By some measures, U.S. public opinion is not particularly negative toward the idea. A Pew Research survey carried out last August found that 46 percent of adults approved of genetic modification of babies to reduce the risk of serious diseases.

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The William Davidson Talmud The William Davidson Talmud

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The William Davidson Talmud is a free digital edition of the Babylonian Talmud with parallel translations, interlinked to major commentaries, biblical citations, Midrash, Halakhah, and an ever-growing library of Jewish texts. As with all of Sefaria, The William Davidson Talmud will continually evolve as we add additional translations, commentaries, and connections. , , , . , , .

The William Davidson Talmud includes Rabbi Adin Even-Israel Steinsaltzs complete Modern Hebrew and English translations of the Talmud. Through the generous support of The William Davidson Foundation, these translations are now available with a Creative Commons non-commercial license, making them free for use and re-use even beyond Sefaria. . , Creative Commons, .

December 5, 1922 - March 13, 2009 5 , 1922 - 13 , 2009

William Davidson was an internationally recognized businessman and lifelong philanthropist who cherished above all else his family and his Jewish heritage. Throughout his life, he supported projects and organizations that preserved and enhanced Jewish life and continuity. From his parents he learned that success in business provides the means for helping others. This family philosophy shaped his life. " , . , . . .

An expert in making troubled companies profitable, he transformed a struggling, family-owned automotive-glass fabricating firm into one of the worlds largest manufacturers of architectural and automotive glass. He also was the owner of several professional sports teams, including the Detroit Pistons basketball team. A native of Detroit, Michigan, he earned his bachelor of business degree from the University of Michigan and his law degree from Wayne State University. , -, , - . , , . , , .

In 2005, he established the William Davidson Foundation, a private, family foundation to advance for future generations the economic, cultural and civic vitality of Southeast Michigan, the State of Israel and the Jewish community. One of the secrets to a fulfilling life, he once said, is to be able to do things for other people. To commemorate his life and extend his spirit of generosity, the Talmud, as translated in English and Modern Hebrew by Rabbi Adin Even-Israel Steinsaltz, is now accessible to all. " 2005, , , - , , . , , . , , (), .

. . . Moses charged us with the TeachingAs the heritage of the congregation of Jacob.Deuteronomy 33:4 :

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How Automation Can Bridge The Gap Between Internal Operations And Customer Service Excellence – Forbes

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How Automation Can Bridge The Gap Between Internal Operations And Customer Service Excellence  Forbes

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The Global Industrial Automation And Control Systems Market size is expected to reach $301.8 billion by 2028, rising at a market growth of 10.0% CAGR…

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The Global Industrial Automation And Control Systems Market size is expected to reach $301.8 billion by 2028, rising at a market growth of 10.0% CAGR during the forecast period  Yahoo Finance

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A startup says its begun releasing particles into the atmosphere, in an effort to tweak the climate – MIT Technology Review

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A startup says its begun releasing particles into the atmosphere, in an effort to tweak the climate  MIT Technology Review

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Emma Thompson On Longevity Of Holiday Fave Love Actually But It Is SEX Actually That Is Earning Her Awards Buzz In Leo Grande – Deadline

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Emma Thompson On Longevity Of Holiday Fave Love Actually But It Is SEX Actually That Is Earning Her Awards Buzz In Leo Grande  Deadline

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14 Romantic Resorts with Private Plunge Pools – Islands

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Theres a reason Do Not Disturb signs were created. When a refreshing dip in a plunge pool is just steps from your bed, theres no need to fight the crowd for a lounge chair or even don a swimsuit. At these resorts with private pools, thats exactly the lifestyle you can enjoy with your significant other.

From the Caribbean and Mexico to Spain, South Africa and even Utah, theres a host of luxurious hotels with private pools perfect for the most relaxing, most romantic getaways. Isolate yourselves at Amangiri in the seemingly remote landscape of Utah; there are sunsets and gorgeous horizons all around. Prefer something more tropical? Visit St. Lucia and Jade Mountain for vistas overlooking the island.

If youre looking for hotel rooms with a private pool, read on for our list of the best 14 locations.

The 24 suites, aptly called sanctuaries at Jade Mountain, are stacked atop each other on a hillside, all with heart-stopping views. As you open the door, get ready to be wowed: Organic architecture blends curved stone walls with exotic hardwood floors, and the open plan means youre never without a view, from the king-sized bed draped with mosquito netting to the sans-walls bathroom with its sexy whirlpool tub.

But the stars are the pools. Sized from 400 to 900 square feet, each is lined with iridescent glass tiles in shades like sapphire, jade, magenta and bronze. The effect is so mesmerizing you wont mind that theres no TV (or air conditioning, as the lofty locale provides cooling breezes). Just pour yourselves some bubbly, and slip into your infinity-edge paradise.

Each romantic vale is nestled into hillside foliage with plunge pools facing out toward the lagoon for ultimate privacy at Royal Davui Island Resort. Trading up from the island or deluxe categories to premium earns you the largest and most private oasis with its own refreshing rock waterfall.

Inside, a 20-foot netted canopy hangs from the ceiling to cocoon the king bed, which floats mid-room on a sea of polished Fijian-wood flooring adjacent to a large bathroom with a whirlpool tub and outdoor shower. A separate living room has sliding glass walls and a sunning patio with twin chaise lounges.

This 539-room all-inclusive is definitely abuzz, but the quiet Grand Class section perfectly combines luxe amenities with honeymoon privacy. All 90 suites in this section have ocean views, spa tubs and oceanfront balconies with private plunge pools. Theres even room for a massage table, should you prefer your spa treatment in suite. Top-floor rooms boast vaulted ceilings and the best views.

Yes, Hermitage Bay has cottages set right on the beach. But if its privacy and plunge pools you seek, request one of 17 hillside-cottage suites. You have to trek up a steep stone path to get there, but your effort is rewarded with the treehouse-like enclave, boasting sexy extras like a king bed, outdoor shower, wraparound sun deck and infinity-edge plunge pool with views that stretch all the way to neighboring Nevis and Montserrat.

Each of the 66 rooms and suites features a private terrace with a plunge pool, but, for the most indulgent ambience, book one of the eight Capella rooms at the Resort at Pedregal. These oversized havens have carved, wood-beam ceilings, a separate sitting area and a driftwood-back king bed positioned opposite a fireplace. The terrace door opens to an infinity-edge plunge pool with stellar sea views. More watery revelry is on tap inside in the rainforest shower for two.

Cavo Tagoo stays true to the stark-white Cycladic architecture Mykonos is known for, but the whitewashed interiors are refreshingly modern. Classic rooms on the lower level have private terraces and refreshing plunge pools while the upper-level deluxe rooms come with infinity-edge pools overlooking the Aegean Sea. A free shuttle runs between the hotel and Old Town five minutes away.

All of the resorts plush, overwater bungalows offer dive- or step-in access to the lagoon, but couples who book an overwater bungalow with a plunge pool can also take a freshwater dip on the lanai right outside their room. Its perfect for a relaxing rinse after a snorkel session or a sunset soak complete with champagne.

Inside, contemporary Polynesian dcor is equally seductive, especially the oversized tub with lagoon views. The glass panel in the living room floor lets you spy on multicolored reef fish below.

For a romantic respite, book a bilevel spa-pool villa at Banyan Tree Mayakoba. Here, your rectangular H2O oasis is the perfect relaxation spot after a massage in your private treatment area. The dcor is Asian spare meets Mexican spice. Think minimalist architecture that blends into the landscape and a neutral color palette accented by touches of orange and red.

Your outdoor terrace has twin chaise lounges and a dining area. Round-the-clock room service ups your incentive to stay put, too.

St. Lucia oozes romance, and the 32 treehouse-style rooms at Ladera match that vibe perfectly. Situated on a ridge 1,100 feet above sea level, the three-walled suites boast panoramic views of the Caribbean, the Pitons (two mountainous volcanic plugs) and the town of Soufriere. Each terrace has a private plunge pool surrounded by lush landscaping. Many also have waterfalls and teak swing sets hanging from the ceiling.

This all-inclusive, 32-room boutique resort on Grenadas southwest coast is on prime real estate, steps away from expansive Grand Anse Beach. The resorts 1,200-square-foot Anthurium pool suites are located on the lower level, and each has a private courtyard terrace where gardens and chaise lounges surround a supersized 12-by-16-foot plunge pool.

There are just three Uma pool suites at COCO Uma Ubud. Theyre constructed in an open, airy Balinese style with separate lounging, dining and sleeping areas. Each bedroom features a four-poster king bed and gleaming terrazzo floor that leads to a light-filled bathroom with a sunken tub for two. Outside, a pair of chaises flanks a sapphire-blue infinity plunge pool embraced by the solitude of the Balinese jungle.

The isle of Majorca is classic Mediterranean: olive groves, quaint towns and seaside cliffs. Within the sleepy artists village of Dei is Belmond La Residencia, a pair of stunning stone houses that have been converted into a 67-room Orient Express retreat. Here, guests lounge around the grounds, hike to the nearby swimming cove or hole up in a Tramuntana suite with a tiled garden terrace and private pool.

The stunning Utah landscape is best experienced at Amangiri, a 34-room oasis surrounded by stark plateaus near the Arizona border and Grand Staircase-Escalante National Monument. Six of the hotels suites have spacious plunge pools and private, candlelit sky terraces accessed by staircase and furnished with a daybed. Its perfect for evening stargazing.

Spend your mornings spotting lions, giraffes and elephants from a Land Rover while on safari around Kruger National Park. Then, return to Earth Lodge for afternoons staring out over the bush from the pool on your private terrace. The lodges 13 suites were made from stone and built low to the ground. While they appear minimal on the outside, inside, theyre an interior designers dream.

Each suite is filled with rich metallic art and funky, locally sourced furnishings, such as handmade twig chandeliers.

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