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Monthly Archives: June 2021
Breakthrough allows scientists to determine the age of endangered native fish using DNA – The Conversation AU
Posted: June 28, 2021 at 9:50 pm
Identifying the age of animals is fundamental to wildlife management. It helps scientists know if a species is at risk of extinction and the rate at which it reproduces, as well as determining what level of fishing is sustainable.
Determining the age of fish has been difficult in the past primarily involving extracting the inner ear bone, also known as the otolith. Layers of growth in the otolith are counted like rings on a tree to reveal an individuals age. Unless a dead specimen is available, this method requires killing a fish, making it unsuitable for use on endangered populations.
However a non-lethal DNA test developed by the CSIRO enables researchers to determine fish age for three iconic and threatened Australian freshwater species: the Australian lungfish, the Murray cod and the Mary River cod. We outline the technological breakthrough in our research just published.
Our fast, accurate and cost-effective test can be adapted for other fish species. We now hope to share this method to improve the protection of wild fish populations and help promote sustainable fisheries around the world.
Human activity has led to the population declines of the three Australian fish species at the centre of our research.
The threatened Australian lungfish is found in rivers and lakes in southeast Queensland. Its often referred to as a living fossil because its extraordinary evolutionary history stretches back more than 100 million years, before all land animals including dinosaurs.
Man-made barriers in rivers reduce the movement of water, which lowers lungfish breeding rates.
Older lungfish do not have hard otolith structures, which makes determining their age difficult. Bomb radiocarbon, which analyses carbon levels in organic matter, has been used to age Australian lungfish, but this method is too expensive to be widely used.
The threatened Murray cod is Australias largest freshwater fish. The Mary River cod is one of Australias most endangered fish, found in less than 30% of its former range in Queenslands Mary River.
Habitat destruction and overfishing are major threats to Murray cod and Mary River cod populations.
Otoliths can be used to determine age for both these cod species, however this has only been done on a population-wide scale for the more prevalent Murray cod.
Read more: Australia's smallest fish among 22 at risk of extinction within two decades
When cells divide to make new cells, DNA is replicated. This can lead to DNA methylation, which involves the addition or the loss of a methyl group molecule at places along the DNA strand.
Research has found the level of DNA methylation is a reliable predictor of age, particularly in mammals, including humans.
To develop our test, we first worked with zebrafish. This species is useful when studying fish biology because it has a short lifespan and high reproductive rates. We took zebrafish whose ages were known, then removed a tiny clip of their fin. We then examined DNA methylation levels in the fin sample to identify the fishs age.
Following this successful step, we transferred the method to Australian lungfish, Murray cod and Mary River cod. Again, we used fish of known ages, as well as bomb radiocarbon dating of scales and ages determined from otoliths.
We found despite the zebrafish and the study fish species being separated by millions of years of evolution, our method worked in all four species. This suggests the test can be used to predict age in many other fish species.
Read more: Good news from the River Murray: these 2 fish species have bounced back from the Millennium Drought in record numbers
In the same way human population demographers use census data to understand and model human populations, we now have the tools to do this with animals.
We are looking to expand this DNA-based method to determine the age of the endangered eastern freshwater cod and trout cod. We will also continue to test the method across other species including reptiles and crustaceans.
This work is part of CSIROs ongoing efforts to use DNA to measure and monitor the environment. This includes estimating the lifespan of vertebrate species such as long-lived fish and surveying biodiversity in seawater using DNA extracted from the environment.
We envisage that in the not too distant future, these methods may be used by other researchers to better understand and manage wild animal populations.
Read more: A new study shows an animal's lifespan is written in the DNA. For humans, it's 38 years
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Breakthrough allows scientists to determine the age of endangered native fish using DNA - The Conversation AU
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Classic Trail-Themed Colorways Appear On The adidas UltraBOOST 5.0 DNA – Sneaker News
Posted: at 9:50 pm
The term DNA at least in the Ultra Boosts case encompasses much more than the design of the shoe itself. And as it joins the 5.0 iteration, the moniker brings along an array of classic, trail-inspired colorways.
We can expect at least three iterations to surface in the next few days, each vivid as it mimics some of the best outdoors gear. The first, which is arguably the most bold of the trio, renders its knits in a dual-tone of light and dark blue; brighter red fixtures dress the branding adjacent, while the sole features a familiar speckling. Opposite, the Wonder White pair is far more muted as it joins alongside cream threads and a warm and cool color combo. Finally, the last rounds us out with a mix replete with Grey Three, Sonic Ink, and Solar Gold pops.
For a closer look at the range, see the official images below. A release, according to adidas AU, is due for June 30th; expect an adidas.com drop in the US to follow.
In other news, the adidas Trae Young 1 is coming soon.
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Classic Trail-Themed Colorways Appear On The adidas UltraBOOST 5.0 DNA
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How researchers in Utah are leading the way in collecting DNA samples to solve sexual assault cases – The Denver Channel
Posted: at 9:50 pm
SALT LAKE CITY, Utah Researchers in Utah are leading the way when it comes to collecting DNA in sexual assault cases. At this moment, Utah is the only state using touch DNA to find answers, and the forensic nurse at the forefront is trying to change that.
Dr. Julie Valentine, a forensic nurse and nursing professor at Brigham Young University in Utah, explains the groundbreaking case that changed the way some DNA evidence is collected.
In 2011, there was a case on a campus where a young woman was violently assaulted and groped," Valentine said When the nurse walked into the room to greet the patient, she was finishing a sandwich and a soda, and anytime you activate the salivary enzymes you destroy the DNA. So, the nurse thought, 'Well, I know that our crime lab has these improved DNA analysis methods."
Valentine is referring to touch DNA, which is DNA from skin cells. Traditionally, it hasnt been used in sexual assault and groping cases.
Well, it turned out we got a full STR DNA profile of the suspect," Valentine said.
That means they were able to identify the assailant. Dr. Valentine says this was a turning point.
"If you are a victim of a groping or a sexual assault and youre thinking oh the person didnt ejaculate or there was no bodily fluid, that does not mean we cant collect evidence," Valentine said.
Monica Garder is a survivor of sexual assault, abuse, and adulthood rape. While she knows who her assailants are, they were never prosecuted for their actions.
"Its dulled me down from the bright light that I used to be to someone thats just barely surviving," Gardner said. And Ive had months and months of being suicidal every day. Ive had times where even in my room with the door locked, Im pacing my room because I feel unsafe.
Gardner knows the pain of sexual assault too well, but technology developed in Utah is giving her hope. She is putting her bravery on display in an effort to help other survivors.
I think there is something that is validating in that to say here is another step, another piece that says Im telling the truth," Gardner said.
Every perpetrator in a sexual assault case leaves behind answers; answers researchers in Utah are helping to find.
I had a case where a victim had been taken by two men and one man held her down on her upper body while she was raped by the other man," Valentine said.
She says both men were identified using Touch DNA.
Dr. Valentine is now working with the National Institute for Justice to create best practice guidelines for touch DNA in sexual assault and groping cases. Throughout the last four years, she has created a series of webinars and presentations to teach professionals in other states and countries about how they too can use touch DNA in these cases.
When DNA tests first came out, you needed about a quarter size of bodily fluid," Valentine said. But thats just not the case; the science has advanced.
They also developed a touch DNA form to help nurse examiners collect evidence, and its the first of its kind. So far, the researchers have found a match in about 21% of cases that utilized Touch DNA.
"It really is this new tool in the toolbox for investigations, but we also have to be cautious and always look at everything in context and look at this as a piece of the investigation and the prosecution," Valentine said.
Unfortunately, touch DNA is only part of the puzzle. Another piece is testing a significantly higher number of rape kits and understanding their scientific value.
The NIJ explains that untested rape kits are a national problem. There is no national system for collecting rape kit data, making it nearly impossible to know how bad the problem really is. There are numerous states where limited reform has been enacted, but there is still much more to do.
Thats why Valentine and the rest of her team are continuing to work behind the scenes to change the future for survivors like Garder. This technology was not around to help in Garder's case, but she's hopeful it will help others get justice.
"This is going to empower the survivor to say, 'No, I can prove you were there. I can prove you touched my body because there is DNA evidence,'" Gardner said.
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Gould’s mouse was declared extinct, but DNA shows it still lives on an island in Shark Bay, Western Australia – ABC News
Posted: at 9:50 pm
Before European settlers set foot on Australian shores, Gould's mouse scurried about thecontinent.
Butland clearing and the introduction of predatorsdecimated native rodent populations, and the little mouse was declared extinct more than a century ago until now.
A team of researchers, led by Emily Roycroft of the Australian National University, looked at genomes of Australia's extinct and living rodents.
Their analysis showsa small population ofGould's mouse (Pseudomys gouldii) lives on an islandoff the coast ofWestern Australia.
But thislittle animal with shaggy fur and large black eyes is knownby another name.
Gould's mouse isactually the samespecies as the Shark Bay mouse (Pseudomys fieldi), they reporttoday in the journalProceedings of the National Academy of Sciences.
"I was quite surprised," Dr Roycroft said.
"Extinction doesn't usually offer second chances."
Australia is home to some of the world's most unique wildlife, with 86per cent of its mammals notfound anywhere else.
But it hasalso lost more mammals over the past 200 years than any other country, with 34 land species being declared extinct since Europeans set foot on the continent in 1788.
Native rodents make up nearly half of these extinctions.
Wikimedia Commons: John Gould
Their rapid decline was likely due to a mix of human impacts.
Along with land clearing and introducing predators such as cats and foxes, early settlers were also keen bounty hunters.
"Basically, the government paid people to bring in dead animals," she said.
And while Gould's mouse was once widespread, it was one of the hardest hit speciesduring European colonisation and hasn't been seen on the mainland since 1895.
But what hasn't been clear is whether Australia's rodents were already struggling before the first settlers arrived.
Dr Roycroft and her colleagues wanted to look at this group's genetic diversitybefore its numbers took a nosedive nearly 200 years ago.
Conservationists often use a population's genetic diversity as a measure of resilience against stressors such as disease and environmental change.
Having a large mix genes gives a species a better chance of adapting to its changing environment.
To assess the genetic diversity of Australia's rodents prior to European settlement, the researchers extracted DNA fromeight extinct rodent species and 42 of their living relatives from museum specimens.
The specimens had been collected between 120 and 184 years ago.
Supplied: C.Ching, Courtesy of the Trustees of the Natural History Museum, London
In the Museums VictoriaAncient DNA Laboratory, the researchers sequenced the specimens' genomes to look at how they're related and how their genetic makeup had changed over time.
They found that the rodents' genomes were diverse before European settlement, indicating that their once-thriving populations were more likely wiped out by human impacts than genetic weakness, such as inbreeding.
The rodents' healthy genomes suggested they declined rapidly, unlike other extinct mammals such as the Tasmanian tiger.
The team also found that larger species were more likely to become extinct.
This is because larger rodents tend to be more attractive to predators and produce fewer young than their smaller relatives.
But there was one extinct species that stood out.
When the researchers analysed the genome of the Gould's mouse specimen, they found that it matched up with a species currently living on the islandsoff Shark Bay in Western Australia.
This means the mousenever completely died out to begin with.
This came as a surprise, as Gould's mouse specimens were originally collected in New South Wales and Victoria over 4,000 kilometres away from Shark Bay.
"That's a really big distance,so I was surprised that those things were essentially the same species," Dr Roycroft said.
"But this has revealed that it was in fact a once very widespread single species that had collapsed down into this single off-shore population."
But how did these mice get there in the first place?
Dr Roycroft said they likely made their way over when the islands were still connected to the mainland thousands of years ago.
"Slowly as the sea level rose, a little pocket of the population may have got trapped there," she said.
Euan Ritchie, a wildlife ecologist at Deakin University, said that it's "fantastic news" that Gould's mouse is aliveon an island in Shark Bay.
But becausethe species is listed as endangered, it's important to make it a conservation priority, said Dr Ritchie, who was not involved in the study.
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"Given this species occurs in such an isolated and single location, establishing captive breeding colonies and additional wild populations should be a priority," he said.
Dr Ritchie also said the study highlightedthe need for more investment in surveying, cataloguing and describing Australia's wildlife.
"We risk being unaware of the true diversity of Australia's species, and hence unable to care for and conserve it properly," he said.
"We essentially risk losing what we don't even know exists."
The next step for Dr Roycroft and her teamis to analyse the genomes of other native animals to look at how they were also impacted by European settlers.
"I hope this study can be an inspiration to looking at other species and seeing what we can learn about Australia's biodiversity that we've lost," Dr Roycroft said.
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adidas Gears Up For The Olympics With The UltraBOOST 4.0 Medal Pack – Sneaker News
Posted: at 9:49 pm
As we inch closer to the Tokyo Olympics, sportswear brands are celebrating with a slew of footwear releases that reference the international event. adidas, for instance, will be following up the success 2016s pack of medal-inspired adidas UltraBOOSTs by recruiting the relatively new adidas UltraBOOST 4.0 DNA.
This new Medal pack consists of three colorways that take after the gold, silver, and bronze medals presented at the Olympics. Each pair consists of a Primeknit upper comprised of a single wash of colorCloud White, Core Black, and Ice Purplewith the TPU lace cages and inside in a matching hue. By adding a touch of award-reminiscent metallics to just the heel counter, the style maintains a versatile, lifestyle-friendly look that still commemorates the worlds largest sporting event. As usual, a BOOST foam midsole and black Continental rubber underfoot rounds out the medalworthy pack.
Take a closer look the UltraBOOST 4.0 DNA Medal pack below, in which the gold and silver pairs are confirmed to drop on adidas.com on July 1st at a price of $180. While details regarding a stateside release for the bronze style have yet to arise, the pair is likely to alongside the two aforementioned pairs with the same price.
For more by the Three Stripes, check out BAITs One-Punch Man-themed adidas Montral 76.
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adidas Gears Up For The Olympics With The UltraBOOST 4.0 Medal Pack - Sneaker News
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McConnell Warns Spending Initiatives ‘Playing Russian Roulette’ With The Economy – WUKY
Posted: at 9:49 pm
Sen. Mitch McConnell is sounding cautious about throwing his support behind a bipartisan infrastructure packagewarning that the ambitious federal spending programs sought by the Biden administration present their own kind of threat to the country.
"We are playing Russian Roulette with the American economy," the GOP leader counseled, taking a question on proposed free community college tuition on Monday.
The answer got to the heart of the sentor's stated unease with everything from the evolving infrastructure agreement to the $300-a-month enhanced federal jobless benefits. The Republican minority leader said the U.S. is running up a dangerously high debt, the size of the economy itself, and its time to get back to work.
"Last year we had a 100-year pandemic... understandable, an emergency. The pandemic is essentially in the rear-view mirror and to continue to borrow and spend at this level is completely unacceptable for the future of this country," McConnell stressed.
But the nations ballooning debt has spanned several administrations, rising an estimated $7.8 trillion under former President Donald Trump.
Right now, the focus is on a fragile bipartisan infrastructure deal running nearly $1 trillion. McConnell said hes encouraged by President Bidens walking back of a threat to veto a standalone bill not attached to his larger social spending agenda. But the senator is still wary, telling reporters he wants assurances from Democratic congressional leaders that the two major spending projects wont be linked. McConnell said he hopes a deal can be hashed out.
McConnell did acknowledge that cutting off the extra pandemic-driven federal unemployment payments would not solve "all the problems" related to hiring shortages, but suggested that "of the things that are available that you could do immediately, it would surely be a huge help."
Democratic Gov. Andy Beshear has opted to try a $1,500 back-to-work incentive program to bolster recruitment efforts, but has not ruled out ending the enhanced benefits early.
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McConnell Warns Spending Initiatives 'Playing Russian Roulette' With The Economy - WUKY
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The ‘bonkers’ world of John McAfee: Russian roulette, poisoned dogs and life on the run from a ‘hit squad’ – The Telegraph
Posted: at 9:49 pm
The voice on the end of the line was somewhere in the Belize jungle and sounded more than a little paranoid.
"I cannot talk on this phone for long," John McAfee told the Telegraph, while on the run after his next-door neighbour was found dead with a bullet in his head.
McAfee said he was being tracked by a paramilitary hit squad and he was in disguise, staying on the move. He said it would be "foolish" to say more.
"They nationalised the phone company so they can trace calls and triangulate," he said. "My plan is a day-to-day plan, simply to avoid detection."
While maintaining his innocence he vowed to never turn himself in, adding: "I think I will be summarily executed. They can make up some excuse for removing me. This is the way it is in this country.
"I would describe myself as quite sane and lucid, which is why I'm still alive. The world chooses to think what the world thinks."
In passing, he denied suggestions he had been running a meth lab.
"There was no meth lab, what on Earth would I be doing running a meth lab?" he said.
It was November 2012 and McAfee had been declared a "person of interest" in the killing of his neighbour Gregory Viant Faull, a retired American builder from Florida.
The two men lived 300 yards from each other on Ambergris Caye, an island off Belize.
Faull was discovered by his housekeeper lying face up in a pool of blood inside his house. A single Luger 9mm shell was found nearby. There was no sign of a break in but a laptop computer and phone were missing.
Faull had previously made a complaint to local authorities about McAfee's dogs, claiming they were "vicious" and attacked people.
Four of the dogs, named Mellow, Lucky, Dipsy, and Guerrero, had been found poisoned the day before Faull was shot dead.
However, McAfee told the Telegraph he did not blame Faull for the demise of his dogs. Instead, he accused the Belize authorities of killing them.
McAfee said: "He (Faull) did not like my dogs. All neighbours can be minor annoyances from time to time but without them the world would be empty. I know he didn't kill my dogs."
On the night of Faull's death he had been "at home", McAfee said, adding: "I heard nothing. I knew nothing until the following day."
About a month later McAfee was arrested in Guatemala for crossing the border illegally. He was deported to the US and was never charged or questioned over the incident in Belize.
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These are the staple games at any online casino – Illinoisnewstoday.com
Posted: at 9:49 pm
Online casinos have never been so popular, with new sites and providers popping up almost every day. Players can see roulette sites with welcome bonuses at the click of a button, and with the popularity of this pastime, it is no surprise that several online casinos have seen a huge increase in revenue and user base over the last 18 months. It is quite interesting to note the similarities between these sites, and one thing that is clear is that no online casino will operate without the big three games in place roulette, poker and slots. One of the biggest advantages of online casinos is that their costs are far lower than that of brick and mortar casinos, and thus they can offer far better odds than physical casinos, making them more attractive.
Poker
One big advantage regarding online poker is that online poker rooms allow players to play for low stakes and often have poker freeroll tournaments where there is no entry fee, which attracts beginners and low-baller clients. Poker games in online casinos are also much faster, since shuffling and dealing are instant, while chip counting is also automatic. On average, online poker tables can have around 90-100 hands per hour, while an offline casino will probably only get through 30 hands in an hour. Online poker is cheaper to play than conventional poker. While the rake structures of online poker sites might not differ from those in brick and mortar operations, most of the other incidental expenses entailed by playing in a live room do not exist in online poker. An online poker player can play at home and incur no transportation costs to and from the poker room. Provided the player already has a computer and an Internet connection, there are no up-front equipment costs to get started. There are also considerable incidental expenses at a live poker table. Besides the rake, tipping the dealers, chip runners, servers, and other casino employees is expected, putting a further drain on a players profits. Also, while an online player can enter and leave tables almost as they please, once seated at a live table a player must remain there until they wish to stop playing or else go back to the bottom of the waiting list. Food and beverages at casinos are expensive even compared to other hospitality establishments in the same city, let alone at home, and casino managers have little incentive to provide complimentary food or drink for poker players.
Roulette
Many of the same advantages apply here as well, with speed, low costs and convenience being the biggest reasons for the popularity of online roulette.
Slots
One of the biggest advantages of online slots is that the outcome of these games is determined using a pseudorandom number generator (PRNG) software. This software ensures that every deal of the card, the outcome of a dice throw, or the results produced by the spinning of a slot machine or roulette wheel is totally random and unpredictable. This is true for roulette games as well. When implemented correctly, a PRNG algorithm will ensure that the games are both fair and unpredictable.
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Judicial deference is alive and well in the Commonwealth of Massachusetts – JD Supra
Posted: at 9:49 pm
For most of my legal career people have been predicting an end to the doctrine of judicial deference that has, since the early 1980s, been the law in Federal Courts, and in Massachusetts. That brings me to the Supreme Judicial Court's decision Friday upholding the Massachusetts Energy Facilities Siting Board's approval of a new electricity transmission line under a railroad right of way passing through the Town of Sudbury.
The Town and other opponents appealed the approval, preferring no additional transmission at all, or at least an alternative route. The Commonwealth's highest court said its job wasn't to substitute its judgments for those of the Legislature or the Executive Branch, only to prevent "patently wrong, unreasonable, arbitrary, whimsical, or capricious" government actions.
The Sudbury project's opponents lawyers had to know all of this going in. If so, they had to be hoping against hope that the Court would deviate from its precedential course, kind of like that last turn at the roulette wheel. Perhaps this renewed certainty about what courts will and won't do will refocus attention on the branches of our government that make the laws and implement them.
The board's interpretation of its statutory mandate will be disturbed only if the interpretation is patently wrong, unreasonable, arbitrary, whimsical, or capricious. Brockton Power Co., supra at 219 , quoting Box Pond Ass'n, 435 Mass. at 416 . As for [*8] the application of facts, [o]ur review does not turn on whether, faced with the same set of facts, we would have drawn the same conclusion as [the siting board], but only whether a contrary conclusion is not merely a possible but a necessary inference. Alliance to Protect Nantucket Sound, Inc. v. Energy Facilities Siting Bd., 457 Mass. 663 , 690 , 932 N.E.2d 787 (2010) (Alliance II),
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Judicial deference is alive and well in the Commonwealth of Massachusetts - JD Supra
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Quik Gaming debuts in the UK follow UKGC approval – Casino Beats
Posted: at 9:49 pm
The UK Gambling Commission has granted its licence approval to Quik Gaming, which will see UK online casino operators integrate Quiks titles.
Debuting in the market, Quik Gaming, which boasts its one-of-a-kind unique Live Games that are available in both single and live player formats, has lauded the approval as a huge step for the company.
Timothy Batolo Parnis, COO at Quik Gaming, noted: The UKGC is known to have strict regulation requirements to comply with. We cant wait to see the joy of players faces as they interact with our games for the first time.
The approval, which comes after a long, thorough preparation exercise to meet the stringent criteria set by the UK gambling regulations, will expand Quiks market reach to one of the largest markets in Europe.
The company highlighted that the feedback from the UK market has already been very encouraging, with operators and partners said to instantly acknowledge the freshness of Quiks product offerings and its appeal to the British market.
We have a long line of parties interested in our innovative Unique Live Games, added Parnis as he announced that online casino operators in the UK will shortly be able to integrate Quiks games to make them available for their wide variety of players for the first time.
At the start of 2021, Quik Gaming launched SuperBloc, which is believed would transform traditional Live Roulette into a sophisticated and entertaining casino adventure.
The first release in a new suite of titles for the Malta Gaming Authority licensed casino games provider, SuperBlocs carries the foundation of Live Roulette, but is built on with an innovative in-game feature and full-blown live bonus game.
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Quik Gaming debuts in the UK follow UKGC approval - Casino Beats
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