Daily Archives: February 26, 2017

Would You Want a Dog That Was Genetically Engineered to Be Healthier? – Singularity Hub

Posted: February 26, 2017 at 10:50 pm

Dogs have more genetic diseases than any other species on the planet. David Ishee told me this early in our conversation. His claim makes sense: theres no other animal that humans have purposefully bred with an emphasis on form over functionaesthetics over healthfor so long.

Centuries of inbreeding have left many dog breeds with a severely limited gene pool, and this lack of genetic diversity is to blame for disorders like brachycephaly in bulldogs, hyperuricemia in dalmations, and cardiomyopathy in boxers.

Ishee is a breeder from rural Mississippi whos on a mission to change all this.

Up until now, hes been using selective breeding to do so. His first big project involved mastiffs, which were once considered a warrior breed, but devolved into the docile, lazy pets we see today. They lost their health, their drive and their athleticism, Ishee said. My project as a breeder is to bring all that back to them.

He envisioned an ideal mastiff: 150-170 pounds, 30+ inches at the shoulders, tight-skinned, dry-mouthed, and free of inherited health problems. Eight years later, this super-mastiff (which is really just a return to what nature intended a mastiff to be) has become a reality.

Ishee has plans to expand his health-focused breeding to other dogs, but he wants a better tool to do so.

Enter gene editing.

Youd think that to tweak the genome of an animal, some serious training and education would be necessarymaybe a post-graduate biology degree or several years working in the lab of a large genetics company.

But in a prime example of both the democratization and demonetization of technology, Ishee taught himself to do genetic engineering right in his own backyard shed, using a kit and some DNA he ordered online.

I think every dog breeder wants better tools than just breeding. But everybody assumes its impossible, or crazy expensive, so I never considered actually trying, Ishee said. That changed after he saw a TED talk about genetic engineering, namely because of the ease and low cost of ordering custom synthesized DNA.

He continued, The biggest thing here is the collapsing price of DNA sequencing and synthesis. You can order synthetic DNA for about nine cents a base pair. When I ordered my construct a year and a half ago, I paid 23 cents a base pair. Six years before that it wouldve been $1.30 a base pair. When it gets down to pennies, people will be able to do much more complex things.

Its this very idea, thoughof anyone being able to do complex gene editing at home with supplies ordered onlinethats caused a tightening of regulations, most recently from the FDA.

As of mid-January, the FDA updated its guidance for animals produced using genome editing to classify the edited portion of the animals genome as a veterinary drug. This means the animals themselves are subject to the same regulations as new animal drugs.

While acknowledging that genome editing technology could have potentially profound beneficial effects on human and animal health, the FDA statement also mentions possible unwanted impacts on the environment and ecosystem, as well as on individual genomes.

The new guidelines threw a wrench into Ishees next project: to use gene editing to rid dalmations of hyperuricemia. A mutation on the dogs' SLC2A9 gene leads to excess uric acid in the blood, which causes painful bladder stones to form and can even cause the bladder to burst.

To breed out this mutation, a breeder would have to wait for a positive mutation to randomly appearand since its a closed and inbred population, that could take decades. CRISPR gene editing could do it in months.

Besides a series of complex approvals and permissions, though, the FDA regulations also involve hefty fees, reaching into six figures per animal. Its a sum thats feasible for a large corporation, but not so much for a breeder. No large corporation is likely to take on projects similar to Ishees, though, because theres not much money to be made. If pet owners are happyor unawareas is, why invest in creating healthier dogs?

The FDA guidance points out that gene editing has raised fundamental ethical questions about human and animal life. Its these questions that are at the root of the knee-jerk negative reaction many have when it comes to these topics. If we allow gene editing to cure diseases in dogs, will a slippery slope ensue? Will I be able to custom-design my pet in the future, and if yes, is that a good thing or a bad thing?

Perhaps most significantly, if breeders like Ishee can prove that CRISPR is an easy way to rid dogs of health issues, will that pave the way for the technology to be more widely used on humans? Scientists in China produced gene-edited beagles in 2015, with one scientist saying their research had biomedical ends, as Dogs are very close to humans in terms of metabolic, physiological, and anatomical characteristics.

In Ishees opinion, genetic engineering and selective breeding arent all that different. CRISPR doesnt allow us to do anything we couldnt do before. Its just a bit easier, cheaper and faster, he said. Breeding gives you a lot less control and fewer degrees of freedom. But as far as the ethics is concerned, youre doing the exact same thing.

Speaking of ethicsif humans caused dogs to have all these health problems in the first place, and now we have the technology to fix those problems, dont we have a moral obligation to do so?

English bulldogs are riddled with serious health problems, from breathing difficulties to hip dysplasia to skin allergies. A study on the breed published in Canine Genetics and Epidemiology stated, The loss of genetic diversity and extreme changes in various regions of the genome will make it very difficult to improve breed health from within the existing gene pool.

The studys co-author told BBC News: "If you want to rebuild the breed, these are the building blocks you have, but they're very few. So if you're using the same old bricks, you're not going to be able to build a new house."

Ishee could be the first breeder to make brand-new bricksif the FDA lets himand other breeders would likely follow close behind.

As CRISPRs costs continue to go down, interest in the technology will go up, hatching hundreds of new backyard biohackers with ideas and projects of their own. Regulatory bodies will have to walk a fine line between protecting against misuse of the technology and allowing experimentation that could benefit both animals and humans.

That experimentation could just as easily be done by our next-door neighbor as by a government agency. Its an idea that will take some getting used to. As Ishee put it:

When you think about genetic engineering, you think of PhDs in white coats working in multi-million-dollar labs. The idea of a dog breeder in rural Mississippi doing genetic engineering in his shed is insane. But thats how you know youre in the future, right?

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Genetics Are the New Eugenics: How GMO’s Reduce the Human Population – Center for Research on Globalization

Posted: at 10:50 pm

The following is from an interview transcript

Last year, we had a series of mergers in the agribusiness GMO-corporations worldwide. This has created an alarming concentration of corporate power in the hands of basically three corporate groups.

The first one is Bayer AG of Germany, which made a friendly takeover of Monsanto. The reason for this was that Monsanto became identified in the public mind as pure evil and everything bad about GMOs, which was accurate. This became a burden on the whole GMO project. So, Bayer stepped in, which has a friendly image of an aspirin, harmless, nice company, but in fact is the company that invented heroin in the 1880s and made gas for the ovens of Auschwitz during WWII. Its one of the dirtiest agribusiness companies in the world with a series of homicides and pesticides that killed off bee colonies and many other things that are essential to life and to nature.

Flickr.com/Miran Rijavec (public domain)

ChemChina China State Chemical giant for some reason took over Swiss Syngenta, which makes weed-killers.

Then, Dow Chemicals and DuPont merged their GMO businesses together.

So, we have three gigantic corporate groups worldwide controlling the genetically-modified part of the human food chain. As dangerous as the GMO crops are and the more they sell, it is becoming more and more obvious that they are the chemicals that by contract must be applied to those GMO seeds by the corporations. They demand that if you buy roundup ready soybeans or corn, you must use Monsanto (now Bayer) roundup.

Therefore, this is giving more corporate power to the GMO industry than ever before and thats an alarming trend. They are putting pressure on the bureaucracy in Brussels. One example: there was a massive public campaign against the renewal of the license of the European Commission for Glyphosate. Glyphosate is the most widely used weed-killer in the world. Glyphosate is the main ingredient in Monsantos roundup. The other ingredients are Monsantos corporate secret, but the combination of them is one of the most deadly weed-killers.

The World Health Organizations body responsible for assessing genetic dangers made a ruling the last year that Glyphosate was a probable cancer-causing agent.

The license came up for automatic renewal last year a 15-year license. The EU commission for health was prepared to automatically renew it for 15 years. The European Food Safety Authority (EFSA), which is responsible allegedly for the health and safety of European citizens, recommended approval based on a German study by the German Food Safety Agency that was simply lifted 100% from studies given by the private corporation Monsanto! So, the whole chain was corrupt from the beginning and all the information was rigged. In reality tests have shown that in minuscule concentrations, lower than in recommended levels in Europe and in the US, Glyphosate causes kidney disease, liver disease, and other illnesses that are potentially fatal.

Now, Glyphosate has shown up in urine tests, in urban drinking water, in gardens, in ground water and so forth. And that gets into the system of childbearing women, for example, with embryo. Its all in this!

The EU commission, despite a million petitions this is a record setting and despite recommendations from leading scientists around the world to not renew the license, made a compromise under huge industry pressure and renewed it for 18 months. Why did they renew it for that time? Because at the end of 18 months, they were told by Bayer and Monsanto that the takeover of those two giant corporations will be completed and Bayer is going to replace Glyphosate with another, likely more deadly toxin, but not so well-known as Glyphosate. So, they simply bought time. And that is just one example.

This agenda of GMO is not about the health and safety; its not about increasing crop yields thats a lie that has been proven in repeated tests in North America and all around the world. Crop yields for farmers, using GMO plants, may increase slightly for the first 1-2 harvest years, but ultimately decline after 3-4 years. And not only that! Weve been promised by Monsanto and other GMO giants that the use of chemicals will be less, because of these wonderful traits that GMO plants resist. In fact, the weeds become resistant and you have super weeds, which are 5-6 feet in a height and choke out everything. Its a catastrophe. So, farmers end up using added weed killers to kill the super weeds. This whole mad playing around with the genetic makeup of nature is a disaster from the beginning.

The real agenda of GMO, which I have documented in great detail in my book Seeds of Destruction, comes from the Rockefeller Foundation. It comes out of the 1920s-1930s Eugenics movement. The Rockefeller Foundation during the 1930s, right up to the outbreak of World War II when it became politically embracing too, financed the Nazi Eugenics experiments of Kaiser Wilhelm Institute in Berlin and in Munich. Why did they do this? Their goal was the elimination of what they called undesirable eaters. That is called population reduction.

After the war, the head of the American Eugenic Society, who was a good friend of John D. Rockefeller, at the annual conference of the American Eugenic Society said: From today, the new name of eugenics is genetics. Moreover, if you keep that in mind genetic engineering, the Human Genome Project and so forth they all are scientific frauds. Russian scientists have proven that the entire Genome Project utterly disregarded 98% of the scientifically valuable data in favor of 2% that was completely nonsense and a waste of billions of dollars.

Therefore, they have been obsessed with the idea of how to reduce human population in a way that would not be so obvious as simply going out and carrying out mass-sterilization.

Actually, they have done that in Central America together with the World Health Organization by giving certain vaccines that they cooked-up to have abortive effects. Therefore, the women of child-bearing age in Central America were given these vaccines against tetanus. The organization of the Catholic Church became suspicious because the shots were given only to women, not to men. And they found that there was buried in the vaccine an abortive effect that made it impossible for women to conceive and bear children. This is all covert population reduction.

These are the Western patriarchs who believe they are the gods, sitting on the throne with great dignity, controlling mankind. I think they are a bunch of fools, but they have this agenda of genetic manipulation. Its against nature, its chemically unstable. And I have to congratulate the Russian Federation that they had the courage and the moral concern for their own population to ban GMO cultivation across Russia. That was a step forward for mankind. I would hope that Russia will use its influence to get China to do the similar thing, because their agriculture is in dire need of some healthy Russian input. But this step by Russia to make a GMO-free agriculture is a great step for mankind.

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Genetics Are the New Eugenics: How GMO's Reduce the Human Population - Center for Research on Globalization

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Clues to relationship between schizophrenia and rheumatoid arthritis – Science Daily

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Clues to relationship between schizophrenia and rheumatoid arthritis
Science Daily
... if individual genetic variants may exist that could have opposing effects on the risk of schizophrenia and rheumatoid arthritis," said co-senior author Vishwajit Nimgaonkar M.D., Ph.D., professor of psychiatry at Pitt's School of Medicine and human ...

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DNA tests completed in 2000 Valley Center murder case; wife had hoped they would exonerate her – The San Diego Union-Tribune

Posted: at 10:49 pm

A little more than a year ago, a San Diego judge granted a womans request to have evidence from her high-profile murder case tested for DNA, a move the defense hoped would point to someone else as the killer.

The woman was Jane Dorotik, who was convicted in 2001of first-degree murder in the slaying of her husband, Robert. He was strangled at the couples Valley Center home.

Jane Dorotik, now 70, is serving a sentence of 25 years to life in prison.

According to the District Attorneys Office, the items in question a rope used to strangle the husband and fingernail clippings or scrapings from his body were tested by the San Diego County Sheriffs Department Crime Lablate last year.

The results of those tests revealed that DNA found on those items belonged to the victim. Its unclear whether any other contributors were identified.

Whats also unclear is whether this means Dorotiks quest for exoneration has come to an end. She is now represented by attorneys from Loyola Law Schools Project for the Innocent, which is based in Los Angeles.

Reached by phone last week, the attorneys declined to confirm the results of the DNA tests or to discuss the status of Dorotiks case.

No further court hearings have been scheduled in San Diego Superior Court.

Nothing has been filedsince the (DNA) results have come back, said Deputy District Attorney Jill Lindberg, the prosecutor most recently assigned to the case. She said she did not know whether any hearings would be scheduled in the near future.

At the time of the killing, Dorotik wasa high-level executive for a mental health services company. She also raised and trained horses. She and her husband had three adult children.

Dorotik reported her husband missing the evening of Feb. 13, 2000. The last time she saw him, she told authorities, was earlier that daywhen he was getting ready to go jogging.

Robert Dorotiks body was found early the next day, Valentines Day, in a wooded area about two miles from the ranch where the family lived. The body, dressed in running clothes,had been strangled and beaten, authorities said.He was 55.

Jane Dorotikwas arrested a few days later. Detectives found a bloodstained mattress and specks of his blood on the floor, walls and ceiling of the master bedroom, which they said indicated her husband was killed in the house.

After examining the clothing on the body, investigators determined he was likely dressed in the running clothes after he was killed, according to court documents. There were bloodtransfer stains but no spatter stains on his T-shirt. No blood was found on his sweatpants or on his shoes.

Prosecutors relied heavily on circumstantial evidence to provetheir case.Dorotik and her husband were home alone when he was most likely killed. There had also been some discord between the husband and wife, which pointed to a possible motive.

Deputy District Attorney Bonnie Howard-Regan argued to the jury in North Countythat Dorotik killed her husband because she was afraid of losing part of her $118,000 annual salary in a divorce. Robert Dorotik had quit his job as an aerospace engineer to start a business making horse jumps, but it wasnt going well.

Dorotik maintained throughout the trial and afterward that she was innocent, but she didnt know who the real killer was.

San Diego Superior Court Judge Joan Weber, who presided over the trial, has said repeatedly that the most incriminating piece of evidence was Jane Dorotiks bloody thumbprint on a syringe filled with animal tranquilizer that was found in a bathroom next to the master bedroom.

Itwas also Weberwho in November 2015 granted a request filed on Dorotiks behalf to allow post-convictionDNA testing of the rope and fingernail scrapings. Neither of those items had been tested previously.

The judge directed lawyers on both sides of the case to try to agree on which lab wouldperform the tests. But that might have been a task easier said than done.

Last April, Weber ordered the Sheriffs Crime Lab to do the testing, but a month later Dorotik, who by then was representing herself, asked the judge to order the lab to test only half the evidence so the rest could be saved for future testing.

Thatrequest was denied.

Later, after Loyolas Project for the Innocent took over the case, the attorneys clarified her request, saying Dorotik did not want the Sheriffs Crime Lab the same one that conducted the testson all of the evidence used to convict her to be the only lab to test the rope and fingernail evidence.

They said Dorotik was concerned about confirmation bias.

What the Defendant would actually like to request is not that half the sample be shelved for later testing but that an independent lab (unrelated to the criminal investigation of her case and one that is not directly affiliated with law enforcement) conduct independent testing on these important pieces of evidence right away, the attorneys wrote in court documents.

Weber denied the defense motion and confirmed in August that the Sheriffs Crime Lab would do the DNA analysis.

dana.littlefield@sduniontribune.com

Twitter: @danalittlefield

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Scientists Zero In On DNA In Hair Samples With Extraordinary … – CBS Local

Posted: at 10:49 pm

February 26, 2017 12:39 AM By Andria Borba

LIVERMORE (KPIX 5) On the crime show NCIS, one hair leads to a killer. That was once the stuff of fantasy, but no more.

Hair is your crowning glory and soon, thanks to scientists from Lawrence Livermore National Lab, it will provide the evidence necessary to tie you definitively to a crime.

Weve leveraged the fact that protein in your body is essentially an echo of your DNA, says Brad Hart, director of the labs Forensic Science Center. Your DNA is a blueprint for the proteins produced by your body.

In the past, hair evidence was collected at crime scenes but could only be used to identify a suspect with a microscope and an expert opinion comparing samples.

Instead of relying on someones opinion about whether something matches, you can make a measurement, says chemist Deon Anex.

Using only an inch of hair, Hart and Anex can identify the amino acids and peptides that make up your hair. So far, the degree of accuracy is one in a million after the hair is broken down into its parts.

We hope in the future of course, to make that even better, says Anex. If we had a hundred of these identification markers in say an identification panel you might say for hair identification, this would be sufficient to get a unique pattern for a person out of the entire worlds population.

The data that comes out when we do the analysis of a particular hair sample you get peaks and valleys that are displayed here that focuses on each individual peptide that comes out of the mass spectrometer, he says.

The advantage of hair over DNA is how robust it is. Think of the hair balls that live under your bed.

DNA in comparison is very fragile and easily mixed up at a messy crime scene.

You come across a crime scene, you may have a pool of blood, but it may not just be one persons blood the more contributors to that mixture of DNA, the more difficult to figure out whos DNA it was. The nice thing about hair, is that if you find a hair, it only came from one person, says Hart.

Scientists at the lab have been able to test a piece of hair for its markers that is over 250 years old.

Breaking the hair down and getting a profile is almost as quick as Abby on NCIS just 24 to 48 hours with about the same cost as DNA.

It comes into play with connecting suspects to a crime scene, but it would also be very, very important in exonerating people, says Anex.

Up next at the lab will be finding out what treating your hair to the Clairol color wheel means for its genetic breakdown.

Is blonde hair different from brunette hair? If someone had dyed their hair or permed their hair, how do those things affect our abilities to recover these profiles? says Hart.

Andria Borba joined KPIX 5 in October 2013. Born in Modesto, raised in the Central Valley city of Gustine, Andria has a large extended family that stretches from Tomales Bay to the Monterey Bay and all over the San Francisco Bay Area.Andrias last...

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Novel DNA vaccine design improves chances of inducing anti-tumor … – Science Daily

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Tech Times
Novel DNA vaccine design improves chances of inducing anti-tumor ...
Science Daily
Scientists have devised a novel DNA vaccine approach through molecular design to improve the immune responses elicited against one of the most important ...
New DNA Vaccine Designed To Boost Immunity And Fight Off ...Tech Times

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Demi Lovato Reveals Details of Her Ancestry DNA Test: ‘I’m 1 Percent African!’ – PEOPLE.com

Posted: at 10:49 pm

Demi Lovatohas always been one of thosepop stars who is not afraidto share her most personal details with her fans whether it be herhistory of addiction,hermental health, or even her struggles with her weight.

So when the 24-year-oldConfident singer recently received the resultsof an ancestry DNA test, it only made sense that she would share her genetic makeup on social media.

I did a DNA test and found out Im mainly Spanish, with Native American, SCANDINAVIAN (which I had NO idea), Irish, BRITISH. Lovato wrote on Twitter Friday. And Im 1% African!!!!

Lovato then shared a photo of the full test results, explaining in a tweet that she hadnt done a DNA test before and only found out about her ancestry from certain places after speaking with her uncle.

Of course, this being social media, some of her followers were quick to criticize the singer. But not one to let Internet trolls win, Lovato saidthat she didnt mean to offend anyone.

Just thought it was cool and totally random, she wrote. Some of yall are mean af. Twitter sucks.

RELATED VIDEO:Demi Lovato and Boyfriend Guilherme Vasconcelos Share Their Love Fest On Insta!

In addition to her ancestry results, Lovato has a lot to celebrate lately includingnew boyfriendGuilherme Bomba Vasconcelos.

Sheand the Brazilian welterweight, 30,were first linked back in Julywhen aninsider told PEOPLE they had a quick fling that ended shortly after. But the couplerecently rekindled their romance, evenringing in the New Year together.

Vasconcelos posted a touching black and white photo with his girlfriend on Wednesday, holding her tight and smiling at the camera.

The caption read, Baby, along with a red heart emoji.

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DNA of rape victim’s child lands suspect – The Blade – Toledo Blade

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Members of the Toledo Police Cold Case Sexual Assault Task Force say they could find no new evidence to solve a 2000 stranger rape until the victim offered one possibility: her son born from the rape.

On Friday, a Lucas County grand jury indicted Harland Hersey, 33, of Toledo on one count of rape for an April 9, 2000, attack on a teenage girl.

Hersey

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Charles McDonald, an assistant county prosecutor assigned to the task force, said the indictment came after the Ohio Bureau of Criminal Investigation conducted a reverse paternity test on the victims son a test that identified his biological father through DNA.

Its a first for our unit, he said of the reverse paternity test.

Due to the creative and exhaustive techniques used by the investigating officers, a DNA profile was developed sufficient to identify a suspect, Mr. McDonald said.

The 16-year-old victim told police at the time that she and her younger brother had walked to the store and were returning home on Waverly Avenue near Avondale Avenue about 2 p.m. when three young men on a porch called them up and coerced them inside.

Mr. McDonald said that while one of the men allegedly kept the victims brother downstairs, the other two men forced the victim upstairs where she was raped.

After she and her brother were able to flee, they ran home where she called police. The young woman was taken to the hospital where a rape kit was done.

Still, no suspects were ever identified or arrests made.

Nothing ever came of the original investigation, Mr. McDonald said.

An arrest warrant for Mr. Hersey would be issued, police said Friday.

Toledo police Detective Vince Mauro, another member of the task force, said an agent from the Ohio Bureau of Criminal Investigation worked the case and ultimately got a hit from the suspects DNA on the FBIs nationwide database known as the Combined DNA Index System, or CODIS.

For the past few years, the task force has been re-examining some 1,700 unsolved rape cases that date back two decades.

Contact Jennifer Feehan at:jfeehan@theblade.comor 419-213-2134.

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Patterns of protein synthesis associated with increased longevity discovered – Science Daily

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Patterns of protein synthesis associated with increased longevity discovered
Science Daily
The study, which appears in the journal Cell Reports, could help identify new therapeutic targets for potential interventions for human diseases associated with old age, such as type 2 diabetes, cancer, neurodegenerative disorders and cardiovascular ...

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Average life expectancy set to increase by 2030 – Science Daily

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Average life expectancy set to increase by 2030
Science Daily
Scientists once thought an average life expectancy of over 90 was impossible, explained Professor Majid Ezzati, lead researcher from the School of Public Health at Imperial: "We repeatedly hear that improvements in human longevity are about to come to ...
Average Life Expectancy Is Expected to Pass 90 for the First Time EverScienceAlert
Average life expectancy on the rise but the US lags behindNew Atlas
Life expectancy will jump over the next two decades in much of the world but not the USNational Post

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