PUBLIC RELEASE DATE:

25-Aug-2014

Contact: Kathryn Ryan kryan@liebertpub.com 914-740-2100 Mary Ann Liebert, Inc./Genetic Engineering News

New Rochelle, NY, August 25, 2014 Nearly 1 in 6 adults worldwide may suffer from chronic constipation and, over time, the disorder can cause serious complications. Auriculotherapy, a form of acupuncture that involves stimulating targeted points on the outer ear, may help in managing constipation. Evidence from numerous clinical studies published between 2007-2013 that evaluated the effectiveness of auriculotherapy in treating patients with constipation is presented and discussed in a Review article in The Journal of Alternative and Complementary Medicine, a peer-reviewed publication from Mary Ann Liebert, Inc., publishers. The article is available free on The Journal of Alternative and Complementary Medicine website until September 25, 2014.

Li-Hua Yang and coauthors from the Hospital of Nanjing University of Traditional Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, and Southeast University School of Public Health, Nanjing, China, analyzed the results of 17 published studies, comparing the effectiveness of auriculotherapy in managing and relieving constipation and in alleviating symptoms associated with constipation between affected patients and a control group. The authors present their data and conclusions in the article "Efficacy of Auriculotherapy for Constipation in Adults: A Systematic Review and Meta-Analysis of Randomized Controlled Trials".

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About the Journal

The Journal of Alternative and Complementary Medicine is a monthly peer-reviewed journal publishing observational, clinical, and scientific reports and commentary intended to help healthcare professionals and scientists evaluate and integrate therapies into patient care protocols and research strategies. Complete tables of content and a sample issue may be viewed on The Journal of Alternative and Complementary Medicine website.

About the Publisher

Mary Ann Liebert, Inc., publishers is a privately held, fully integrated media company known for establishing authoritative peer-reviewed journals in many promising areas of science and biomedical research, including Alternative and Complementary Therapies, Medical Acupuncture, Brain and Gut, and Journal of Medicinal Food. Its biotechnology trade magazine, Genetic Engineering & Biotechnology News (GEN), was the first in its field and is today the industry's most widely read publication worldwide. A complete list of the firm's 80 journals, books, and newsmagazines is available on the Mary Ann Liebert, Inc., publishers website.

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Can auriculotherapy help relieve chronic constipation?

DID ALIENS CREATE HUMANS? (EXTRATERRESTRIAL GENETIC ENGINEERING DOCUMENTARY)
DID ALIENS CREATE HUMANS? (EXTRATERRESTRIAL GENETIC ENGINEERING DOCUMENTARY) Evidence clearly indicates that modern man is not the result of a linear evoluti...

By: ParaNormality

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DID ALIENS CREATE HUMANS? (EXTRATERRESTRIAL GENETIC ENGINEERING DOCUMENTARY) - Video

By Tom Abate

Stanford bioengineer Christina Smolke has been on a decade-long quest to genetically alter yeast to "brew" opioid medicines in stainless steel vats, eliminating the need to raise poppies.

For centuries poppy plants have been grown to provide opium, the compound from which morphine and other important medicines such as oxycodone are derived.

Now bioengineers at Stanford have hacked the DNA of yeast and reprogrammed these simple cells to make opioid-based medicines via a sophisticated extension of the basic brewing process that makes beer.

Led by bioengineering Associate Professor Christina Smolke, the Stanford team has already spent a decade genetically engineering yeast cells to reproduce the biochemistry of poppies, with the ultimate goal of producing opium-based medicines, from start to finish, in fermentation vats.

"We are now very close to replicating the entire opioid production process in a way that eliminates the need to grow poppies, allowing us to reliably manufacture essential medicines while mitigating the potential for diversion to illegal use," said Smolke, who outlines her work in the Aug. 24 edition of Nature Chemical Biology.

In the new report, Smolke and her collaborators, Kate Thodey, a postdoctoral scholar in bioengineering, and Stephanie Galanie, a doctoral student in chemistry, detail how they added five genes from two different organisms to yeast cells. Three of these genes came from the poppy itself, and the others from a bacterium that lives on poppy plant stalks.

This multi-species gene mashup was required to turn yeast into cellular factories that replicate two, now separate processes: how nature produces opium in poppies, and then how pharmacologists use chemical processes to further refine opium derivatives into modern opioid drugs such as hydrocodone.

Morphine is one of three principal painkillers derived from opium. As a class they are called opiates. The other two important opiates are codeine, which has been used as a cough remedy, and thebaine, which is further refined by chemical processes to create higher-value therapeutics such as oxycodone and hydrocodone, better known by brand names such as OxyContin and Vicodin, respectively.

Today, legal poppy farming is restricted to a few countries including Australia, France, Hungary, India, Spain and Turkey supervised by the International Narcotics Control Board, which seeks to prevent opiates like morphine, for instance, from being refined into illegal heroin.

Originally posted here:
Stanford bioengineers close to brewing painkillers without opium from poppies

ICGEB Neurobiology Lab takes up the Ice Bucket Challenge for a world without ALS
http://www.alsa.org/fight-als/ice-bucket-challenge.html.

By: International Centre for Genetic Engineering and Biotechnology

Link:
ICGEB Neurobiology Lab takes up the Ice Bucket Challenge for a world without ALS - Video

During the latter stage stages of the 20th century, man harnessed the power of the atom, and not long after, soon realised the power of genes. Genetic engineering is going to become a very mainstream part of our lives sooner or later, because there are so many possibilities advantages (and disadvantages) involved. Here are just some of the advantages :

Of course there are two sides to the coin, here are some possible eventualities and disadvantages.

Genetic engineering may be one of the greatest breakthroughs in recent history alongside the discovery of the atom and space flight, however, with the above eventualities and facts above in hand, governments have produced legislation to control what sort of experiments are done involving genetic engineering. In the UK there are strict laws prohibiting any experiments involving the cloning of humans. However, over the years here are some of the experimental 'breakthroughs' made possible by genetic engineering.

Genetic engineering has been impossible until recent times due to the complex and microscopic nature of DNA and its component nucleotides. Through progressive studies, more and more in this area is being made possible, with the above examples only showing some of the potential that genetic engineering shows.

For us to understand chromosomes and DNA more clearly, they can be mapped for future reference. More simplistic organisms such as fruit fly (Drosophila) have been chromosome mapped due to their simplistic nature meaning they will require less genes to operate. At present, a task named the Human Genome Project is mapping the human genome, and should be completed in the next ten years.

The process of genetic engineering involves splicing an area of a chromosome, a gene, that controls a certain characteristic of the body. The enzyme endonuclease is used to split a DNA sequence and split the gene from the rest of the chromosome. For example, this gene may be programmed to produce an antiviral protein. This gene is removed and can be placed into another organism. For example, it can be placed into a bacteria, where it is sealed into the DNA chain using ligase. When the chromosome is once again sealed, the bacteria is now effectively re-programmed to replicate this new antiviral protein. The bacteria can continue to live a healthy life, though genetic engineering and human intervention has actively manipulated what the bacteria actually is. No doubt there are advantages and disadvantages, and this whole subject area will become more prominent over time.

The next page returns the more natural circumstances of genetic diversity.

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Genetic Engineering Advantages & Disadvantages - Biology ...

20 hours ago

It is possible to apply genetic modification to the crop crambe so it meets market demands and takes into account what modern society wants. These are the results of research for which Weicong Qi will receive his PhD on 27 August at Wageningen University, part of Wageningen UR.

Qi has developed a technique to genetically modify crambe without marker genes appearing in the crop. Using this technique he was able to steer the manufacture of metabolic substances in a direction that would make the crop more attractive to the processing industry.

Crambe (Crambe abyssinica), a bio-based economy crop, is a source of renewable raw materials that will allow industry to become less dependent on petroleum, thus leading to a more sustainable economy.

Biobased Economy

At the moment, however, the quantities of oil this crop produces are too small to meet the needs of industry, which has a particular interest in erucic acid. That is why crambe has been bred for a number of decades. It seems that when Crambe is crossbred, it can produce, at most, 65% erucic acid: too little to make the crop economically viable. That is why the Chinese PhD student Weicong Qi investigated the possibility of enhancing erucic acid production in crambe by applying genetic modification.

Genetically modifying crambe

Qi has developed a workable system for genetically modifying crambe, and one that does not lead to marker genes in the crop. He used the technique to transfer a gene to crambe which meant the plant could build oil molecules with not two, but three erucic acid molecules on the three available positions on each oil molecule (glycerol). In this way Qi managed to increase the erucic acid levels to more than 75%. According to his co-supervisor, Frans Krens, this is an important step forward. "It's not the whole story, but Qi has shown that this approach can work. A start has been made"

Explore further: Research uses camelina to build better biofuel

A Kansas State University biochemist is improving biofuels with a promising crop: Camelina sativa. The research may help boost rural economies and provide farmers with a value-added product.

The rest is here:
Crambe could be an even better oil crop thanks to gene technology

Aug 20, 2014 The green anole lizard (Anolis carolinensis), when caught by a predator, can lose its tail and then grow it back. Researchers have discovered the genetic 'recipe' as to how this happens. Credit: Joel Robertson

By understanding the secret of how lizards regenerate their tails, researchers may be able to develop ways to stimulate the regeneration of limbs in humans. Now, a team of researchers from Arizona State University is one step closer to solving that mystery. The scientists have discovered the genetic "recipe" for lizard tail regeneration, which may come down to using genetic ingredients in just the right mixture and amounts.

An interdisciplinary team of scientists used next-generation molecular and computer analysis tools to examine the genes turned on in tail regeneration. The team studied the regenerating tail of the green anole lizard (Anolis carolinensis), which when caught by a predator, can lose its tail and then grow it back.

The findings are published today in the journal PLOS ONE.

"Lizards basically share the same toolbox of genes as humans," said lead author Kenro Kusumi, professor in ASU's School of Life Sciences and associate dean in the College of Liberal Arts and Sciences. "Lizards are the most closely-related animals to humans that can regenerate entire appendages. We discovered that they turn on at least 326 genes in specific regions of the regenerating tail, including genes involved in embryonic development, response to hormonal signals and wound healing."

Other animals, such as salamanders, frog tadpoles and fish, can also regenerate their tails, with growth mostly at the tip. During tail regeneration, they all turn on genes in what is called the 'Wnt pathway'a process that is required to control stem cells in many organs such as the brain, hair follicles and blood vessels. However, lizards have a unique pattern of tissue growth that is distributed throughout the tail.

"Regeneration is not an instant process," said Elizabeth Hutchins, a graduate student in ASU's molecular and cellular biology program and co-author of the paper. "In fact, it takes lizards more than 60 days to regenerate a functional tail. Lizards form a complex regenerating structure with cells growing into tissues at a number of sites along the tail."

"We have identified one type of cell that is important for tissue regeneration," said Jeanne Wilson-Rawls, co-author and associate professor with ASU's School of Life Sciences. "Just like in mice and humans, lizards have satellite cells that can grow and develop into skeletal muscle and other tissues."

"Using next-generation technologies to sequence all the genes expressed during regeneration, we have unlocked the mystery of what genes are needed to regrow the lizard tail," said Kusumi. "By following the genetic recipe for regeneration that is found in lizards, and then harnessing those same genes in human cells, it may be possible to regrow new cartilage, muscle or even spinal cord in the future."

The researchers hope their findings will help lead to discoveries of new therapeutic approaches to spinal cord injuries, repairing birth defects, and treating diseases such as arthritis.

Continue reading here:
How lizards regenerate their tails: Researchers discover genetic 'recipe'

7 hours ago

(Phys.org) There's an old saying in the biofuels industry: "You can make anything from lignin except money." But now, a new study may pave the way to challenging that adage. The study from the Energy Department's National Renewable Energy Laboratory (NREL) demonstrates a concept that provides opportunities for the successful conversion of lignin into a variety of renewable fuels, chemicals, and materials for a sustainable energy economy.

"Lignin Valorization Through Integrated Biological Funneling and Chemical Catalysis" was recently published in the Proceedings of the National Academy of Sciences. The NREL-led research project explores an innovative method for upgrading lignin.

The process for converting glucose from biomass into fuels such as ethanol has been well established. However, plants also contain a significant amount of lignin up to 30 percent of their cell walls. Lignin is a heterogeneous aromatic polymer that plants use to strengthen cell walls, but it is typically considered a hindrance to cost-effectively obtaining carbohydrates, and residual lignin is often burned for process heat because it is difficult to depolymerize and upgrade into useful fuels or chemicals.

"Biorefineries that convert cellulosic biomass into liquid transportation fuels typically generate more lignin than necessary to power the operation," NREL Senior Engineer and a co-author of the study Gregg Beckham said. "Strategies that incorporate new approaches to transform the leftover lignin to more diverse and valuable products are desperately needed."

Although lignin depolymerization has been studied for nearly a century, the development of cost-effective upgrading processes for lignin valorization has been limited.

In nature, some microorganisms have figured out how to overcome the heterogeneity of lignin. "Rot" fungi and some bacteria are able to secrete powerful enzymes or chemical oxidants to break down lignin in plant cell walls, which produces a heterogeneous mixture of aromatic molecules. Given this large pool of aromatics present in nature, some bacteria have developed "funneling" pathways to uptake the resulting aromatic molecules and use them as a carbon and energy source.

This new study shows that developing biological conversion processes for one such lignin-utilizing organism may enable new routes to overcome the heterogeneity of lignin. And, that may enable a broader slate of molecules derived from lignocellulosic biomass.

"The conceptual approach we demonstrate can be applied to many different types of biomass feedstocks and combined with many different strategies for breaking down lignin, engineering the biological pathways to produce different intermediates, and catalytically upgrading the biologically-derived product to develop a larger range of valuable molecules derived from lignin," Beckham said. "It holds promise for a wide variety of industrial applications. While this is very exciting, certainly there remains a significant amount of technology development to make this process economically viable."

A patent application has been filed on this research and NREL's Technology Transfer Office will be working with researchers to identify potential licensees of the technology.

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New process helps overcome obstacles to produce renewable fuels and chemicals

3 hours ago by David Stacey

(Phys.org) A problem that has puzzled canola breeders for years has been solved by researchers from The University of Western Australia - and the results could provide a vital breakthrough in understanding the impact of increasing global temperatures on crop flowering.

The key to understanding what makes Australian canola flower earlier than its Canadian and European counterparts lies in the genes.

Associate Professor Matthew Nelson from UWA's Institute of Agriculture and School of Plant Biology has identified that heat-responsive genes are responsible for flowering time in Australian spring-type and European summer-type canola. This is the first time such genes have been reported to influence flowering time in canola.

Australian canola is quite distinct from its Canadian and European counterparts - it flowers much earlier. Plant breeders cannot simply transfer varieties from Canada or Europe into Australia as they flower much too late for the Australian environment.

"We took a European summer-type canola, crossed it with Monty, a typical early flowering Australian variety, and analysed the progeny for variation in flowering time," Associate Professor Nelson said.

"There was a huge variation from about 30 days to 160 days in our typical Australian environment. This was totally unexpected and we showed there are several forms of these heat-responsive genes controlling flowering time."

The research indicated that the European plants required much more accumulated heat (thermal time) to flower than the Australian plants.

"Until now, most researchers assumed that long summer days in Europe and Canada triggered flowering, not heat," Associate Professor Nelson said. "Now we know that long days are only a minor part of the story."

"Understanding this complex process is important as breeders alter the adaptation of crops to a new and changing environment," research team leader Winthrop Professor Wallace Cowling said. "International canola breeders will use this information to re-establish the correct flowering time in canola when they cross between Australian types and summer annual types in the northern hemisphere.

Link:
Canola flowers faster with heat genes

PUBLIC RELEASE DATE:

21-Aug-2014

Contact: Kathryn Ryan kryan@liebertpub.com 914-740-2100 Mary Ann Liebert, Inc./Genetic Engineering News

New Rochelle, NY, August 21, 2014Popular fiction that normalizes and glamorizes violence against women, such as the blockbuster Fifty Shades series, may be associated with a greater risk of potentially harmful health behaviors and risks. The results of a provocative new study are presented in the article "Fiction or Not? Fifty Shades Is Associated with Health Risks in Adolescent and Young Adult Females," published in Journal of Women's Health, a peer-reviewed publication from Mary Ann Liebert, Inc., publishers. The article is available free on the Journal of Women's Health website.

Amy Bonomi and coauthors from Michigan State University (East Lansing, MI), Group Health Research Institute (Seattle, WA), and Ohio State University (Columbus, OH) compared young women ages 18-24, readers versus non-readers of at least the first novel in the Fifty Shades series based on self-reports of intimate partner violence victimization (including shouting, swearing, delivering unwanted calls or text messages, and other forms of verbal/emotional abuse, stalking, as well as physical and sexual abuse), binge drinking, disordered eating (use of diet aids and fasting for more than 24 hours), and sexual practices such as number of intercourse partners during their lifetime. The findings point to a substantially greater risk for certain adverse health behaviors among the group that read Fifty Shades, which hyper-sexualizes women and may reaffirm and create the context for those behaviors.

"Clearly, we need a better understanding of the association between reading popular fiction that depicts violence towards women and engaging in risky health behaviors, particularly among adolescent and young adult women," says Susan G. Kornstein, MD, Editor-in-Chief of Journal of Women's Health, Executive Director of the Virginia Commonwealth University Institute for Women's Health, Richmond, VA, and President of the Academy of Women's Health.

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About the Journal

Journal of Women's Health, published monthly, is a core multidisciplinary journal dedicated to the diseases and conditions that hold greater risk for or are more prevalent among women, as well as diseases that present differently in women. The Journal covers the latest advances and clinical applications of new diagnostic procedures and therapeutic protocols for the prevention and management of women's healthcare issues. Complete tables of content and a sample issue may be viewed on the Journal of Women's Health website. Journal of Women's Health is the official journal of the Academy of Women's Health and the Society for Women's Health Research.

About the Society

Continue reading here:
Women's health and Fifty Shades: Increased risks for young adult readers?

PUBLIC RELEASE DATE:

21-Aug-2014

Contact: Kathryn Ryan kryan@liebertpub.com 914-740-2100 Mary Ann Liebert, Inc./Genetic Engineering News

New Rochelle, NY, August 21, 2014--Mesenchymal stem cells (MSCs) are present in virtually every type of human tissue and may help in organ regeneration after injury. But the theory that MSCs are released from the bone marrow into the blood stream following organ damage, and migrate to the site of injury, has long been debated. M.J. Hoogduijn and colleagues provide conclusive evidence to resolve the controversy over the mobilization and migration of MSCs in humans in a new study published in Stem Cells and Development, a peer-reviewed journal from Mary Ann Liebert, Inc., publishers. The article is available on the Stem Cells and Development website.

In "No Evidence for Circulating Mesenchymal Stem Cells in Patients with Organ Injury," Hoogduijn and coauthors from Erasmus University Medical Center (Rotterdam, The Netherlands), describe the results of studies to detect MSCs in the blood of healthy individuals, of patients with end-stage renal disease, of patients with end-stage liver disease, and of heart transplant patients with organ rejection. Whereas they did not find MSCs in the circulation of these individuals, they did report the presence of MSCs in the blood of a patient suffering from severe trauma with multiple fractures. In the trauma patient, the circulating MSCs likely derived from disruption of the bone marrow caused by the fractures.

"We can add the simple but elegant work of Martin Hoogduijn to the pantheon of studies in stem cell research that skewer a long treasured tenet of faith and consign it to mythology," says Editor-in-Chief Graham C. Parker, PhD, The Carman and Ann Adams Department of Pediatrics, Wayne State University School of Medicine, Detroit, MI.

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About the Journal

Stem Cells and Development is an authoritative peer-reviewed journal published 24 times per year in print and online. The Journal is dedicated to communication and objective analysis of developments in the biology, characteristics, and therapeutic utility of stem cells, especially those of the hematopoietic system. A complete table of contents and free sample issue may be viewed on the Stem Cells and Development website.

About the Publisher

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Conclusive evidence on role of circulating mesenchymal stem cells in organ injury

PUBLIC RELEASE DATE:

19-Aug-2014

Contact: Kathryn Ryan kryan@liebertpub.com 914-740-2100 Mary Ann Liebert, Inc./Genetic Engineering News

New Rochelle, August 19, 2014A new ovoid structure discovered in the Nakhla Martian meteorite is made of nanocrystalline iron-rich clay, contains a variety of minerals, and shows evidence of undergoing a past shock event from impact, with resulting melting of the permafrost and mixing of surface and subsurface fluids. Based on the results of a broad range of analytical studies to determine the origin of this new structure, scientists present the competing hypotheses for how this ovoid formed, point to the most likely conclusion, and discuss how these findings impact the field of astrobiology in a fascinating article published in Astrobiology, a peer-reviewed journal from Mary Ann Liebert, Inc., publishers. The article is available Open Access on the Astrobiology website.

In the article, "A Conspicuous Clay Ovoid in Nakhla: Evidence for Subsurface Hydrothermal Alteration on Mars with Implications for Astrobiology," Elias Chatzitheodoridis, National Technical University of Athens, Greece, and Sarah Haigh and Ian Lyon, the University of Manchester, UK, describe the use of tools including electron microscopy, x-ray, and spectroscopy to analyze the ovoid structure. While the authors do not believe the formation of this structure involved biological materials, that is a possible hypothesis, and they note that evidence exists supporting the presence of niche environments in the Martian subsurface that could support life.

"This study illustrates the importance of correlating different types of datasets when attempting to discern whether something in rock is a biosignature indicative of life," says Sherry L. Cady, PhD, Editor-in-Chief of Astrobiology and Chief Scientist at the Pacific Northwest National Laboratory. "Though the authors couldn't prove definitively that the object of focus was evidence of life, their research strategy revealed a significant amount of information about the potential for life to inhabit the subsurface of Mars."

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About the Journal

Astrobiology, led by Editor-in-Chief Sherry L. Cady, Chief Scientist at the Pacific Northwest National Laboratory, and a prominent international editorial board comprised of esteemed scientists in the field, is the authoritative peer-reviewed journal for the most up-to-date information and perspectives on exciting new research findings and discoveries emanating from interplanetary exploration and terrestrial field and laboratory research programs. The Journal is published monthly online with Open Access options and in print. Complete tables of content and a sample issue may be viewed on the Astrobiology website.

About the Publisher

See more here:
Life on Mars? Implications of a newly discovered mineral-rich structure

4 hours ago by Keith Hautala

University of Kentucky biologist Randal Voss is sequencing the genome of salamanders. Though we share many of the same genes, the salamander genome is massive compared to our own, about 10 times as large.

Voss's research focuses on axolotls, salamanders with amazing regenerative ability.

"It's hard to find a body part they can't regenerate: the limbs, the tail, the spinal cord, the eye, and in some species, the lens, half of their brain has been shown to regenerate," Voss said."I'm very fortunate to have a colleague in the department, Jeramiah Smith, who's an expert at the ability to put small pieces of DNA together to kind of recreate the puzzle, which is the genome. We have funding from the National Institute of Health and the Department of Defense to sequence the axolotl genome and provide this blueprint for the first time."

With a partner at the University of Dayton, Voss is looking at the loss of regenerative ability in the eye as a salamander ages.

"Early on in life, axolotls can regenerate their lens. But at some point in time, around 28 days after they hatch, that plasticity goes away and they can't regenerate the lens," Voss said. "So, I've been working with that group trying to identify the genes that might explain that."

Voss is also starting a new collaboration with an orthopedic surgeon at UK to study knee joint regeneration.

This video is not supported by your browser at this time.

"Over the course of say 10 to 15 days, the salamander will successfully regenerate a complete joint. That blows the orthopedic surgeon's mind because that would be the Holy Grail in their field to understand how to orchestrate joint regeneration in a human."

Explore further: Researchers cohere research cluster focusing on genetic mechanisms underscoring regeneration

See the rest here:
Sequencing the genome of salamanders

PUBLIC RELEASE DATE:

18-Aug-2014

Contact: Nicole Baritot press@aats.org 978-299-4520 American Association for Thoracic Surgery

Beverly, MA, August 18, 2014 Investigators from the National Heart, Lung, and Blood Institute (NHLBI) of the National Institutes of Health (NIH) have successfully transplanted hearts from genetically engineered piglets into baboons' abdomens and had the hearts survive for more than one year, twice as long as previously reported. This was achieved by using genetically engineered porcine donors and a more focused immunosuppression regimen in the baboon recipients, according to a study published in The Journal of Thoracic and Cardiovascular Surgery, an official publication of the American Association for Thoracic Surgery.

Cardiac transplantation is the treatment of choice for end stage heart failure. According to the NHLBI, approximately 3,000 people in the US are on the waiting list for a heart transplant, while only 2,000 donor hearts become available each year. For cardiac patients currently waiting for organs, mechanical assist devices are the only options available. These devices, however, are imperfect and experience issues with power supplies, infection, and problems with blood clots and bleeding.

Transplantation using an animal organ, or xenotransplantation, has been proposed as a valid option to save human lives. "Until we learn to grow organs via tissue engineering, which is unlikely in the near future, xenotransplantation seems to be a valid approach to supplement human organ availability. Despite many setbacks over the years, recent genetic and immunologic advancements have helped revitalized progress in the xenotransplantation field," comments lead investigator Muhammad M. Mohiuddin, MD, of the Cardiothoracic Surgery Research Program at the NHLBI.

Dr. Mohiuddin's group and other investigators have developed techniques on two fronts to overcome some of the roadblocks that previously hindered successful xenotransplantation. The first advance was the ability to produce genetically engineered pigs as a source of donor organs by NHLBI's collaborator, Revivicor, Inc. The pigs had the genes that cause adverse immunologic reactions in humans "knocked out" and human genes that make the organ more compatible with human physiology were inserted. The second advance was the use of target-specific immunosuppression, which limits rejection of the transplanted organ rather than the usual generalized immunosuppression, which is more toxic.

Pigs were chosen because their anatomy is compatible with that of humans and they have a rapid breeding cycle, among other reasons. They are also widely available as a source of organs.

In this study, researchers compared the survival of hearts from genetically engineered piglets that were organized into different experimental groups based on the genetic modifications introduced. The gene that synthesizes the enzyme alpha 1-3 galactosidase transferase was "knocked out" in all piglets, thus eliminating one immunologic rejection target. The pig hearts also expressed one or two human transgenes to prevent blood from clotting. The transplanted hearts were attached to the circulatory systems of the host baboons, but placed in the baboons' abdomens. The baboons' own hearts, which were left in place, maintained circulatory function, and allowed the baboons to live despite the risk of organ rejection.

The researchers found that in one group (with a human gene), the average transplant survival was more than 200 days, dramatically surpassing the survival times of the other three groups (average survival 70 days, 21 days, and 80 days, respectively). Two of the five grafts in the long-surviving group stopped contracting on postoperative days 146 and 150, but the other three grafts were still contracting at more than 200 to 500 days at the time of the study's submission for publication.

More here:
Pigs' hearts transplanted into baboon hosts remain viable more than a year

19 hours ago A Batwa young man in Bwindi Impenetrable Forest National Park, Uganda. Credit: George Perry, Penn State

The small body size associated with the pygmy phenotype is probably a selective adaptation for rainforest hunter-gatherers, according to an international team of researchers, but all African pygmy phenotypes do not have the same genetic underpinning, suggesting a more recent adaptation than previously thought.

"I'm interested in how rainforest hunter-gatherers have adapted to their very challenging environments," said George H. Perry, assistant professor of anthropology and biology, Penn State. "Tropical rainforests are difficult for humans to live in. It is extremely hot and humid with limited food, especially when fruit is not in season."

A phenotype is the outward expression of genetic makeup and while two individuals with the same phenotype may look alike, their genes may differ substantially. The pygmy phenotype exists in many parts of Africa, Southeast Asia, the Philippines and potentially in South America. The phenotype is usually associated with rainforest hunter-gathers, groups of people who do not farm, but obtain resources by hunting large and small animals and gathering fruit, nuts, insects and other available resources.

Perry and colleagues looked at the genetics of the Batwa rainforest hunter-gatherers of Uganda and compared them to their farming neighbors, the Bakiga, with whom they traditionally traded forest products for grain, and sometimes intermarry. The researchers also looked at the Baka rainforest hunter-gatherers and their farming neighbors the Nzebi/Nzime in central Africa. They report their results online today (Aug. 18) in the Proceedings of the National Academies of Science.

The average height for Batwa men is five foot and for women it is four foot eight inches. Their short stature is not caused by a single genetic mutation as occurs in many forms of dwarfism, but is the result of a variety of genetic changes throughout the genome that influence height.

The researchers investigated 16 different genetic locations that were associated with short stature when they looked at individuals who were an admixture of Batwa and Bakiga. Several of these regions contained genes known to be involved with growth in humans. They then studied these regions to look for indications that the changes were ones that persisted because they were adaptive.

Genetic mutations occur in populations all the time. If they have a negative impact on the individual, they tend to disappear from the population quickly. If they have no noticeable impact for the good or bad, they might disappear as well, although more slowly. Mutations which have positive influence on individuals, making them more fit for their environment, tend to spread through the population.

Short stature may be adaptive for rainforest individuals for a variety of reasons, according to Perry. Small bodies require less food, which is adaptive for a food-limited location like the rainforest. Small bodies also generate less heat, which, in the heat and humidity of the rainforest, is adaptive. It is also easier for small, agile individuals to move through dense undergrowth and to climb trees.

The results of the genetic comparison indicated that there was a statistical difference between the two groups indicative of multi gene adaptation.

Continued here:
Pygmy phenotype developed many times, adaptive to rainforest

3 hours ago San Diego State University graduate student Yan Wei Lim is exploring coral reefs in the southern Line Islands. Credit: Rob Edwards, SDSU computer scientist

Daylight was breaking over the central Pacific and coffee brewing aboard the MY Hanse Explorer. Between sips, about a dozen scientists strategized for the day ahead. Some would don wetsuits and slip below the surface to collect water samples around the southern Line Islands' numerous coral reefs. Others would tinker with the whirring gizmos and delicate machinery strewn throughout the 158-foot research vessel. All shared a single goal: Be the first research group to bring a DNA sequencer out into the field to do remote sequencing in real time. Against an ocean of odds, they succeeded.

This three-week, five-island expedition took place last year with a research crew including San Diego State University computer scientist Rob Edwards, biologist Forest Rohwer, postdoctoral scholar Andreas Haas and graduate student Yan Wei Lim. They were accompanied by several other researchers from the San Diego region and around the world. The researchers published an account of their trip and methods today in the journal PeerJ.

Line Island investigations

Biologists and computer scientists at SDSU have been traveling to the Line Islands for the last decade, collecting and analyzing the coral habitat to better understand what organisms live there, how they compete for resources, and what effects their presence has on the reef's ecosystem. It always bothered Edwards that they had to wait until they were back home, on the other side of the world, before they could look at their data and develop new hypotheses.

"If only we had had that data out in the field, we could have asked those questions there and then," Edwards said.

That inkling grew into an ambitious plan to somehow, some way bring out to sea a cumbersome and expensive piece of equipment designed to analyze a sample's DNA makeup and spit out detailed information about its genome.

The project initially had its doubters.

"People are a little bit hesitant to take a half-million-dollar piece of equipment into the middle of the Pacific if you're not sure it's going to be coming back," Edwards said.

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Sequencing at sea: Real-time DNA sequencing in a remote field location

PUBLIC RELEASE DATE:

18-Aug-2014

Contact: Kathryn Ryan kryan@liebertpub.com 914-740-2100 Mary Ann Liebert, Inc./Genetic Engineering News

New Rochelle, NY, August 18, 2014While much attention has focused on the link between violent video game playing and aggression among youths, a new study finds significantly increased signs of depression among preteens with high daily exposure to violent video games. The details and implications of this important new study are described in Cyberpsychology, Behavior, and Social Networking, a peer-reviewed journal from Mary Ann Liebert, Inc., publishers. The article is available free on the Cyberpsychology, Behavior, and Social Networking website.

Susan R. Tortolero, PhD and coauthors from The University of Texas Health Science Center at Houston School of Public Health, RAND Corporation (Santa Monica, CA), The University of Alabama at Birmingham, Centers for Disease Control and Prevention (Atlanta, GA), and Boston Children's Hospital and Harvard Medical School (Boston, MA) recorded significantly more depressive symptoms over the course of a year among fifth-graders from three U.S. cities who reported playing high-violence video games for 2 or more hours a day, compared to those who reported playing low-violence video games for less than 2 hours a day. This association was consistent across all racial/ethnic subgroups and among boys, according to the study results presented in the article "Daily Violent Video Game Playing and Depression in Preadolescent Youth."

"One of the strengths of this study is its large and ethnically diverse sample," says Editor-in-Chief Brenda K. Wiederhold, PhD, MBA, BCB, BCN, Interactive Media Institute, San Diego, California and Virtual Reality Medical Institute, Brussels, Belgium.

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About the Journal

Cyberpsychology, Behavior, and Social Networking is an authoritative peer-reviewed journal published monthly online with Open Access options and in print that explores the psychological and social issues surrounding the Internet and interactive technologies plus cybertherapy and rehabilitation, plus cybertherapy and rehabilitation. Complete tables of contents and a sample issue may be viewed on the Cyberpsychology, Behavior, and Social Networking website.

About the Publisher

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Are children who play violent video games at greater risk for depression?

20 hours ago Cold Spring Harbor Laboratory researchers have found that miRNAs, short RNA molecules, are responsible for sexual differences in fruit flies. Shown here are testes from a male fruit fly where a hormone that controls a key miRNA has been inactivated. The abnormal testes fail to make sperm. They now produce sex determinants (shown in red) that are found in the ovaries of female flies. Credit: D. Fagegaltier/ Cold Spring Harbor Laboratory

Men and women differ in plenty of obvious ways, and scientists have long known that genetic differences buried deep within our DNA underlie these distinctions. In the past, most research has focused on understanding how the genes that encode proteins act as sex determinants. But Cold Spring Harbor Laboratory (CSHL) scientists have found that a subset of very small genes encoding short RNA molecules, called microRNAs (miRNAs), also play a key role in differentiating male and female tissues in the fruit fly.

A miRNA is a short segment of RNA that fine-tunes the activation of one or several protein-coding genes. miRNAs are able to silence the genes they target and, in doing so, orchestrate complex genetic programs that are the basis of development.

In work published in Genetics, a team of CSHL researchers and colleagues describe how miRNAs contribute to sexual differences in fruit flies. You've probably never noticed, but male and female flies differ visibly, just like other animals. For example, females are 25% larger than males with lighter pigmentation and more abdominal segments.

The team of researchers, including Delphine Fagegaltier, PhD, lead author on the study, and CSHL Professor and Howard Hughes Medical Institute Investigator Greg Hannon, identified distinct miRNA populations in male and female flies. "We found that the differences in miRNAs are important in shaping the structures that distinguish the two sexes," says Fagegaltier. "In fact, miRNAs regulate the very proteins that act as sex determinants during development."

The team found that miRNAs are essential for sex determination even after an animal has grown to adulthood. "They send signals that allow germ cells, i.e., eggs and sperm, to develop, ensuring fertility," Fagegaltier explains. "Removing one miRNA from mature, adult flies causes infertility." More than that, these flies begin to produce both male and female sex-determinants. "In a sense, once they have lost this miRNA, the flies become male and female at the same time," according to Fagegaltier. "It is amazing that the very smallest genes can have such a big effect on sexual identity."

Some miRNAs examined in the study, such as let-7, have been preserved by evolution because of their utility; humans and many other animals carry versions of them. "This is probably just the tip of the iceberg," says Fagegaltier. "There are likely many more miRNAs regulating sexual identity at the cellular and tissue level, but we still have a lot to learn about these differences in humans, and how they could contribute to developmental defects and disease."

Explore further: Scientists identify a gene that controls the timing of precisely ordered events during maturation

More information: "A Genome-Wide Survey of Sexually Dimorphic Expression of Drosophila miRNAs Identifies the Steroid Hormone-Induced miRNA let-7 as a Regulator of Sexual Identity" appeared online in Genetics on July 31, 2014.

Closely related organisms share most of their genes, but these similarities belie major differences in behavior, intelligence, and physical appearance. For example, we share nearly 99% of our genes with chimps, ...

Originally posted here:
More than just X and Y: A new genetic basis for sex determination

1 hour ago Amy Brunner

Two Virginia Tech researchers have received a $1.4 million grant to investigate the genetic regulatory networks that will allow an important bioenergy crop to be bred so it will grow in less than ideal soils and climate.

Populus, a genus of fast-growing trees commonly known as cottonwoods and aspens, is being grown for bioenergy because it produces a significant amount of biomass in two years and will re-grow robustly when cut at just above ground level. Woody biomass can be converted to liquid fuels, such as ethanol.

"The goal is to develop the species so it will not become dormant in conditions that would stress other crops, such as high temperature, drought, or marginal soil nutrients," said Amy Brunner, associate professor of molecular genetics in the College of Natural Resources and Environment and an affiliate of the Fralin Life Science Institute. "It is important that bioenergy crops not require prime agricultural land."

"We don't want biomass production to compete with food production," she continued. "The aim is to minimize inputs, develop varieties that grow in different environments, and maximize biomass production."

Brunner and Jason Holliday, assistant professor of forest genetics and biotechnology in the college and a fellow Fralin Life Science Institute affiliate, received the grant from the U.S. Department of Agriculture National Institute of Food and Agriculture and the U.S. Department of Energy Office of Biological and Environmental Research. Their project is one of 10 grants awarded as part of the national strategy of sustainable biofuels production.

"The college made the decision to enter into the specialized and highly competitive research arena of molecular genetics, and Drs. Brunner and Holliday are making important contributions to the body of molecular genetics science of tree species," said Paul Winistorfer, dean of the college. "Developing alternative approaches to biofuel crops and their adaptation and success to a changing climate is a strategic and important contribution to our future energy needs."

Brunner and Holliday are experimenting with the FT2 gene, which regulates vegetative growth. "In addition to seasonal dormancy, which happens when days get shorter, a common response to stress by woody plants is to stop growing and wait for things to get better, which is important to natural populations' ability to survive adverse conditions," said Brunner.

"Jason and I are melding our expertise to understand growth and dormancy transitions," she continued. "We will identify specific control points that can be manipulated to maximize growth in different environments."

The FT2 gene integrates signals regarding environmental conditions, such as day length and drought, to control shoot growth or regrowth after harvest.

Excerpt from:
Forestry geneticists develop tree biomass crop to grow on marginal lands

Aug 17, 2014 by Nancy Owano

Data, data everywhere and now as ever researchers need the best tools to make the data useful. In medicine, searching through genomic data can take some time. A startup called One Codex hopes to make difference with their genetic search platform that can process data sets quickly. A report on their work on Friday in TechCrunch noted the advantage of One Codex speed. "Currently," wrote Julian Chokkattu, "the most commonly used tool for genome searching is by using an algorithm called BLAST, Basic Local Alignment Search Tool, which compares primary biological sequence information." For Nick Greenfield, cofounder of One Codex, uploading a file to BLAST took two minutes and 30 seconds to process, compared with the One Codex system where the number was less than 1/20th of a second. The company defines One Codex as a search engine for genomic data. The TechCrunch piece describes what they offer as a service platform for genomics. Apart from using search technology," said Chokkattu, the platform also acts as an indexed, curated reference.

The company said that it can search the world's largest index of bacterial, viral, and fungal genomes. A key advantage is speed. The product can, said the company, "process next-generation datasets in minutes, not days (millions of DNA base pairs per second)."

The two founders are Nick Greenfield, former data scientist, and Nik Krumm, who has a PhD in genome sciences from the University of Washington.

Sample applications would be in clinical diagnostics, food safety and biosecurity. Right now, said TechCrunch, the company is focusing on testing their platform with hospitals and agencies. One Codex is in open beta.

Scientific interest in being able to search genomic data faster has been in evidence for some years. In 2012, MIT's news office reported on a study in Nature Biotechnology, where MIT and Harvard researchers described an algorithm "that drastically reduces the time it takes to find a particular gene sequence in a database of genomes. Moreover, the more genomes it's searching, the greater the speedup it affords, so its advantages will only compound as more data is generated."

The authors of that paper, titled "Compressive genomics," said, "In the past two decades, genomic sequencing capabilities have increased exponentially, outstripping advances in computing power. Extracting new insights from the data sets currently being generated will require not only faster computers, but also smarter algorithms." They stated that although compression schemes for BLAST and BLAT that they presented yield an increase in computational speed and in scaling, "they are only a first step."

Explore further: Team develops tool to better visualize, analyze human genomic data

More information: One Codex: onecodex.com/

2014 Phys.org

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One Codex in open beta for genomic data search