In Duke Biology Lab, NCCU Student Get A Chance to Set Goals

Story and Photos by Gabriel Aikens, NCCU Summer intern

The bubbling of reactions and the sight of stern-looking, goggle-wearing scientists with lab coats on the verge of discovering the next big cure is what goes on in Dukes biology labs, right? No, not at all.

Besides seeing lab coats and a variety of beakers, one might be surprised to also find people in hoodies or khaki shorts and sneakers listening to their favorite songs and joking with each other.

Morgan Morrison has been working with the plant Arabidopsis thaliana in the lab of Xinnian Dong in Duke Biology.

Dont be fooled by the relaxed environment however. These graduate and undergraduate students are hard at work, including intern Morgan Morrison, a North Carolina Central University senior from Charlotte interning at Dukes Institute of Genome Sciences & Policy (IGSP) Summer Fellowship Program. Morrison and her colleagues spend their days transferring samples into geno-grinders (machines that grind plant tissue), carefully extracting chemicals with pipettes, and handling subzero nitrogen which sizzles and hisses loudly as samples are lowered into it..

Morrison was accepted into other summer internships but chose Dukes because of her interest in genomics and her attraction to the university.

This internship is offered to a select few students from around the nation to take on various projects. Morrison is undergoing two projects, observing plant-microbe interactions and cloning plants to study their transcription of genes.

Im observing the microbes (microscopic organisms) to find useful plant-derived compounds for combatting infections, says Morrison. Im cloning plants to see how resistant they are to a given disease.

Morrison is doing molecular cloning, which is different from making genetically identical copies like cloning in the movies. Molecular cloning is the engineering of transgenic plants, which are plants containing genes transferred from another species. The plant she works with is Arabidopsis, a small flowering plant thats a member of the mustard family.

To perform molecular cloning, she first identifies a protein of interest (POI) that might confer resistance to the Arabidopsis and then through a series of steps inserts the DNA coding sequence of that POI into the DNA of Arabidopsis. Then she conducts experiments test whether the new protein conferred resistance.

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In Duke Biology Lab, NCCU Student Get A Chance to Set Goals

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