Any scientist can tell you that research is a time-consuming pursuit. In fact, it can take decades to show results, as the knowledge compounds and inspires additional studies. This building of information is what led to the Recombinant Attenuated Salmonella Vaccine or RASV investigation, which launched to the International Space Station on July 8, 2011.
The investigation combines decades of expertise between two Arizona State University research teams. One team, led by Cheryl Nickerson, Ph.D. specializes in the use of the spaceflight platform to provide insight into how microbial pathogens cause infection and disease in the human body. The other team, led by Roy Curtiss III, Ph.D. focuses on the design and clinical testing of next generation vaccines to protect against diseases caused by pathogenic microbes. In addition, the Arizona State University researchers partnered with Mark Ott, Ph.D., at NASA's Johnson Space Center to strengthen the team's core expertise of space microbiology.
The vaccine samples that were flown on STS-135 are a genetically altered strain of Salmonella that carries a protective antigen against Streptococcus pneumonia -- a bacteria that causes life-threatening diseases, such as pneumonia, meningitis, and bacteremia. This organism is responsible for more than 10 million deaths annually and is particularly dangerous for newborns and the elderly, as they are less responsive to current anti-pneumococcal vaccines. "We have the opportunity," commented Nickerson, "to utilize spaceflight as a unique research and development platform for novel applications with potential to help fight a globally devastating disease."
Nickerson and Curtiss designed the RASV experiment to use the unique microgravity environment of the space station National Laboratory to increase the vaccine's anti-pneumococcal effectiveness by maximizing its ability to induce a protective immune response. Already a promising oral vaccine candidate that is in human clinical trials, RASV has many advantages over vaccines delivered by a needle. This includes activation of an additional arm of the immune system that cannot be engaged by vaccines that are administered as a shot. The Salmonella vaccine strain is genetically modified not to cause disease in humans, but instead carries an antigenic protein from Streptococcus pneumonia. This addition stimulates a protective immune response without actually causing the disease.
According to Nickerson, the initial clinical trials indicated a need for additional enhancement to the vaccine's ability to induce a potent protective immune response. By sending samples back to the space station for continued microgravity research, scientists hope that they will be able to better genetically engineer the vaccine strain to enhance its immunogenicity, while reducing or eliminating any unwanted side effects.
To accomplish this goal, special growth chambers containing the vaccine strain traveled to the station aboard the shuttle Atlantis, where crew members activated the samples. Scientists simultaneously are growing a control sample on the ground for comparison under otherwise identical conditions. The spaceflight cultured RASV strain returned to Earth with STS-135 on July 21, 2011.
Researchers will now evaluate the space-flown strain against the control sample for its ability to protect against pneumococcal infection and changes in gene expression. Molecular targets identified from this work hold promise for translation to develop new and improve existing anti-pneumococcal RASVs to prevent disease for the general public. Moreover, because RASVs can be produced against a wide variety of human pathogens, the outcome of this study could influence the development of vaccines against many other diseases in addition to pneumonia.
Early work that laid the foundation for the microgravity RASV investigation began in 1998 when Nickerson initially was funded by NASA. This was the first of what would be multiple studies from this team on Salmonella bacteria grown in true microgravity or ground-based analogues of microgravity. The goal was initially to see how the bacteria would respond to a microgravity environment.
The ground study led to 2006's Effect of Spaceflight on Microbial Gene Expression and Virulence or Microbe investigation. The findings for Microbe were surprising, as scientists discovered that Salmonella cultured in the spaceflight environment became more virulent -- meaning there was an increase in its disease-causing potential. This study also showed that spaceflight globally altered Salmonella gene expression in key ways that were not observed during culture on Earth, leading to the identification of a master switch that regulates this response.
The Nickerson team followed Microbe with 2008's Microbial Drug Resistance and Virulence or MDRV investigation. This study both reproduced the increased virulence effect in spaceflight-grown Salmonella and identified a way to turn off the increased virulence. Collectively, these investigations enabled researchers to devise the RASV flight experiment in an effort to develop a better vaccine against pneumonia. "The key to this research is the novel way that bacterial cells adapt and respond to culture in the microgravity environment," said Nickerson, "as they exhibit important biological characteristics that are directly relevant to human health and disease that are not observed using traditional experimental approaches."
The current investigation is not the final chapter in this journey towards vaccine development. Thanks to the recent signing of a Space Act Agreement between NASA and the Biodesign Institute at Arizona State University, Nickerson and her team are now users of the space station as a National Laboratory. Scientists participating in this study plan to fly a continuing series of experiments to the space station. This streamlined access will help to accelerate progress for this lifesaving vaccine.
For more information visit http://www.nasa.gov/mission_pages/station/research/news/RASV.html
- Fermi Telescope Caps First Year With Glimpse of Space-Time [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- Antarctic Airborne Science Mission Nears Mid-Point [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- NASA Awards Education Research Grants to Minority Universities [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- New Celestial Map Gives Directions for GPS [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- NASA Invites Reporters to Tranquility Node Ceremony at Kennedy [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- Happy Halloween [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- NASA's Fermi Telescope Detects Gamma-Ray From "Star Factories" in Other Galaxies [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- NASA Announces Advisory Council Chairs and Committee Structure [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- NASA and X Prize Announce Winners of Lunar Lander Challenge [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- MESSENGER Spacecraft Reveals More Hidden Territory on Mercury [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- Successful Flight Through Enceladus Plume [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- Education Secretary Hosts DC Students for Talk with Space Station [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- Operation Ice Bridge Studies Antarctic Sea Ice [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- NASA West Point Welcomes Home One of Their Heroes [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- NASA Spitzer Observes a Chaotic Planetary System [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- X-38 Crew Return Vehicle Finds New Home [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- Frost-Covered Phoenix Lander Seen in Winter Images [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- Have some faith He is doing his best [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- The Cross [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- Spring Bloom in New Zealand Waters [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- NASA Hubble image showcases star birth in M83, the Southern Pinwheel [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- Take Me Out to the Ballpark - On Mars! [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- Poisk Poised for Live NASA TV Space Station Docking [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- Ceremony Reset for ESA Handover of Tranquility to NASA [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- NASA Reproduces a Building Block of Life in Laboratory [Last Updated On: November 8th, 2009] [Originally Added On: November 8th, 2009]
- Now Online: Aeronautics Goes E-Book [Last Updated On: December 12th, 2009] [Originally Added On: December 12th, 2009]
- Water on the Moon, Drought on Earth: NASA Experts Available for Radio And Podcast Interviews During Major Science Meeting [Last Updated On: December 12th, 2009] [Originally Added On: December 12th, 2009]
- Launch of NASA's Wise Spacecraft Delayed Until Dec. 14 [Last Updated On: December 12th, 2009] [Originally Added On: December 12th, 2009]
- Connecticut Students Set for Cosmic Conversation with Space Station Commander [Last Updated On: December 12th, 2009] [Originally Added On: December 12th, 2009]
- Magnetic Dance of Titan and Saturn To Be Main Attraction during Flyby [Last Updated On: December 12th, 2009] [Originally Added On: December 12th, 2009]
- Hubble's Deepest View of Universe Unveils Never-Before-Seen Galaxies [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Earth's Moon [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- How do you Make a Helicopter Safer to Fly? You Crash One. [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Physicist Earns Title as Kennedy's Best [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Hubble Unveils Never-Before-Seen Galaxies [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Saturn's Mysterious Hexagon Emerges from Winter Darkness [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Fermi Sees Brightest-Ever Blazar Flare [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Magnetic Power Revealed in Gamma-Ray Burst Jet [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Just 5 Questions: Aerosols [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Hometown Heroes 2009: Astronaut & Terrible Towel Return to Pittsburgh [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Garver Honors Four for Saving the Life of a Fifth at NASA Langley [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Administrator Bolden Speaks at AAIA-WIA Luncheon [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Best (Meteor) Shower of 2009 - No Towel Required [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- NASA Making Government More Accessible With Cutting-Edge Use Of New Media [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Scarce Water, Our Quiet Sun and Space Rocks Among NASA News Highlights at American Geophysical Union Meeting [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Press Credentials Deadlines Set for Next Space Shuttle Flight [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Reddish Dust and Ice Migration Darken Saturn's Moon Iapetus [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Galaxy Collision Switches on Black Hole [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- I’m watching the launch of NASA’s WISE spacecraft [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- SOFIA Aloft [Last Updated On: December 13th, 2009] [Originally Added On: December 13th, 2009]
- Guide to the International Space Station Laboratory Racks Interactive [Last Updated On: December 14th, 2009] [Originally Added On: December 14th, 2009]
- Freezing WISE's Hydrogen [Last Updated On: December 14th, 2009] [Originally Added On: December 14th, 2009]
- Local High School Wins Invention Challenge [Last Updated On: December 14th, 2009] [Originally Added On: December 14th, 2009]
- WISE Ready to Soar Into Space [Last Updated On: December 14th, 2009] [Originally Added On: December 14th, 2009]
- NASA Data Reveal Major Groundwater Loss in California's Heartland [Last Updated On: December 15th, 2009] [Originally Added On: December 15th, 2009]
- NASA Looks for Safer Icing Forecast For Pilots [Last Updated On: December 15th, 2009] [Originally Added On: December 15th, 2009]
- Challenges of Living and Working Aboard the Space Station: NASA Astronaut Nicole Stott Available for TV Interviews [Last Updated On: December 15th, 2009] [Originally Added On: December 15th, 2009]
- NASA Astronaut, Food Scientist Available for Interviews about Holiday Feasts in Space [Last Updated On: December 15th, 2009] [Originally Added On: December 15th, 2009]
- NASA Launches Web Site for Teenagers That Want More Class [Last Updated On: December 15th, 2009] [Originally Added On: December 15th, 2009]
- A Unique Geography -- and Soot and Dust -- Conspire Against Himalayan Glaciers [Last Updated On: December 15th, 2009] [Originally Added On: December 15th, 2009]
- New Study Turns Up the Heat on Soot's Role in Himalayan Warming [Last Updated On: December 15th, 2009] [Originally Added On: December 15th, 2009]
- NASA's WISE Eye on the Universe Begins All-Sky Survey Mission [Last Updated On: December 15th, 2009] [Originally Added On: December 15th, 2009]
- NASA Offers Sound Clips for Radio, Online Newscasters [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- NASA Gets Up-Close Look at Far Corner of the Globe [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- Orion Launch Abort System Attitude Control Motor Test-fired [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- Hubble Finds Smallest Kuiper Belt Object Ever Seen [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- The Dark Side of Carbon [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- R97UYEA6HD8W [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- NASA's AIM Satellite and Models are Unlocking the Secrets of Mysterious "Night-Shining" Clouds [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- Classroom Learning Takes Off with NASA-Funded Education Projects [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- NASA Buys Additional Space Shuttle Reusable Solid Rocket Motors [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- Searching for New Vaccines and Studying Butterflies in Space; NASA Offers TV Interviews about Latest Space Station Science Research [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- NASA Partners with Saudi Arabia on Moon and Asteroid Research [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- New Results from a Terra-ific Decade in Orbit [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- Deposits in Martian Trough Point to Complex Hydrological Past [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- NASA Outlines Recent Greenhouse Gas Research [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- Unexpected Wheel-Test Results [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- Solar Storms and Radiation Exposure on Commercial Flights [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- Global Digital Elevation Model [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]
- Hubble's Festive View of a Grand Star-Forming Region [Last Updated On: December 17th, 2009] [Originally Added On: December 17th, 2009]