N.D. Tiwari, the maverick politician, managed to keep at bay the controversial issue of paternity for decades, using political clout, influence and delaying tactics in courts.

But, a few weeks ago, his lie was finally nailed by technology. DNA fingerprinting technology conclusively proved that the 87-year-old Tiwari, a former chief minister of Uttar Pradesh and Uttarakhand and more recently Governor of Andhra Pradesh, was indeed the biological father of 32-year-old Rohit Shekhar.

Rohit Shekhar filed a case five years ago. Tiwari tried his best to get it dismissed in court, going right up to the Supreme Court. He made valiant attempts to avoid the DNA (deoxyribonucleic acid) test, claiming invasion of his privacy.

But, the dogged pursuit by Rohit and the Courts directive to get the blood sample for the test has finally brought out the truth. The acceptance of DNA fingerprinting as evidence by the courts should be seen as yet another milestone in the evolution of this technology in the country.

The first time that a court accepted DNA fingerprinting result to settle a paternity case was in 1991. It was the result of pioneering work by Lalji Singh and team at the Centre for Cellular and Molecular Biology (CCMB), Hyderabad, who had developed DNA fingerprinting technology in India.

The technique has been successfully deployed in solving some sensational crimes like the killing and roasting of the body of Naina Sahni in a tandoor in a New Delhi restaurant in 1995 as well as in the high profile assassinations of former Prime Minister Rajiv Gandhi and Punjab Chief Minister, Beant Singh. It has been used in identifying criminals, and sorting out immigration problems.

However, unlike in the US and the UK where it is available to the common man, in India its potential has not been exploited adequately, says Singh. In the last two decades, the applications of this technique have been widely demonstrated. It is possible to distinguish between the ivory of an Indian elephant and an African one, clear doubts on contamination of meat, establish the veracity of plant varieties and trace the source of snake venom.

Given the societal and business opportunities it has opened up, DNA fingerprinting should have been available as a simple, affordable test across the country. Efforts in building databanks of populations, screening for diseases, and general use of DNA technology for genetic counselling should have proliferated under a regulatory environment. But growth remains below expectations, say experts.

At present, the CCMB, the Centre for DNA Fingerprinting and Diagnostics (CDFD), and a few forensic laboratories have the DNA sequencers, diagnostic kits, laboratory facilities and trained manpower to undertake the testing. There are hardly any hospitals or diagnostic centres which have ventured into the area.

As a technology, DNA fingerprinting has emerged as a versatile scientific tool. During the early days, one required a few milligrams of the DNA sample for analysis, which took a couple of weeks. The availability of PCR (polymerase chain reaction) technology, automation and growing expertise has now made it possible to do a test in 24 hours. We require a small (nanogram) sample of DNA, even degraded material is enough. The test is less expensive and accuracy beyond doubt, Singh says. The significance lies in the accuracy of the analysis and ensuring quality and regulatory check on the use of the technique, he explains.

Continued here:
DNA fingerprinting comes of age